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    1989 07 Leaf CallusinductioninGliricidiasepium

    Cargado por

    Ricardo Russo, Maestría en Gestión Ambiental
    An experiment was conducted in the greenhouse to define a methodology to obtain complete Gliricidia sepium plants from leaf tissue. BAP (0.2 and 2.0 mg / l) and 2,4-D (0, 1, 3 and 5 mg / l) were used as supplements of growth hormone in culture medium Murashige and Skoog. At 10 days of incubation under constant light at 20 degrees C, the leaf disks grown on the concentration of 1, 3 and 5-D 2.4 mg / l began to curl (no treatment except 2.4 -D). At 20 days, all the leaf discs that were screwed, fo

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    1989 07 Leaf CallusinductioninGliricidiasepium

    Cargado por

    Ricardo Russo, Maestría en Gestión Ambiental
    13 vistas2 páginas
    An experiment was conducted in the greenhouse to define a methodology to obtain complete Gliricidia sepium plants from leaf tissue. BAP (0.2 and 2.0 mg / l) and 2,4-D (0, 1, 3 and 5 mg / l) were used as supplements of growth hormone in culture medium Murashige and Skoog. At 10 days of incubation under constant light at 20 degrees C, the leaf disks grown on the concentration of 1, 3 and 5-D 2.4 mg / l began to curl (no treatment except 2.4 -D). At 20 days, all the leaf discs that were screwed, fo

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    An experiment was conducted in the greenhouse to define a methodology to obtain complete Gliricidia sepium plants from leaf tissue. BAP (0.2 and 2.0 mg / l) and 2,4-D (0, 1, 3 and 5 mg / l) were used as supplements of growth hormone in culture medium Murashige and Skoog. At 10 days of incubation under constant light at 20 degrees C, the leaf disks grown on the concentration of 1, 3 and 5-D 2.4 mg / l began to curl (no treatment except 2.4 -D). At 20 days, all the leaf discs that were screwed, fo

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    13 vistas2 páginas

    1989 07 Leaf CallusinductioninGliricidiasepium

    Cargado por

    Ricardo Russo, Maestría en Gestión Ambiental
    An experiment was conducted in the greenhouse to define a methodology to obtain complete Gliricidia sepium plants from leaf tissue. BAP (0.2 and 2.0 mg / l) and 2,4-D (0, 1, 3 and 5 mg / l) were used as supplements of growth hormone in culture medium Murashige and Skoog. At 10 days of incubation under constant light at 20 degrees C, the leaf disks grown on the concentration of 1, 3 and 5-D 2.4 mg / l began to curl (no treatment except 2.4 -D). At 20 days, all the leaf discs that were screwed, fo

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    Leaf-callus induction in Gliricidia sepium.

    Article · June 1990

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    2 authors:

    Ricardo Russo Graeme P Berlyn


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    Signatura: 37731.
    Autor: RUSSO, R.O.; Berlyn, G.P.
    Título: Leaf-callus induction in Gliricidia sepium.
    Fuente: Nitrogen Fixing Tree Research Reports, 7:103-105.
    Idioma: eng.
    P.imprenta: 1989.
    Descriptores: Gliricidia sepium; Cultivo de tejidos;PROPAGACION
    VEGETATIVA;Biotecnología;Cultivo;Gliricidia;Leguminosas;PROPAGACION (DE
    PLANTAS) Forage; Journal articles; Artículos en revistas; Forrajes
    Resumen: Se realizó un experimento. de invernadero para definir una metodología para obtener
    plantas completas de Gliricidia sepium a partir de tejido foliar. Se utilizaron BAP (0.2 y
    2.0 mg/l) y 2,4-D (0, 1, 3 y 5 mg/l) como suplementos de la hormona de crecimiento en
    medio de cultivo Murashige y Skoog. A los 10 días de incubación en condiciones de luz
    constante a 20 grados C, los discos foliares cultivados en las concentración de 1, 3 y 5 mg
    de 2,4-D/l comenzaron a enroscarse (con excepción del tratamiento sin 2,4-D). A los 20
    dias, todos los discos foliares que se habían enroscado, formaron callos. A los 40 dias,
    todos los callos se pesaron individualmente y los discos se trasfirieron a un medio sin 2,4-
    D y con diferentes concentración de BAP para inducir organogénesis. La frecuencia de
    regeneración de callos fue de 60-80 por ciento. En ausencia de 2,4- D, no se formaron
    callos. Se concluye que el primer paso del cultivo de tejidos de G. sepium va en la
    dirección correcta. Se obtuvo un cultivo libre de gérmenes, con alta frecuencia y rápido
    crecimiento de callos. La razón optima auxina:citoquinina fue 2.5:1. Se recomienda probar
    diferentes concentraciones de 2,4-D comprendidas entre 0 y 1 mg/l, para encontrar el nivel
    límite de respuesta a la auxina. (CIAT).

    Abstract: An experiment was conducted in the greenhouse to define a methodology to obtain


    complete Gliricidia sepium plants from leaf tissue. BAP (0.2 and 2.0 mg / l) and 2,4-D (0,
    1, 3 and 5 mg / l) were used as supplements of growth hormone in culture medium
    Murashige and Skoog. At 10 days of incubation under constant light at 20 degrees C, the
    leaf disks grown on the concentration of 1, 3 and 5-D 2.4 mg / l began to curl (no
    treatment except 2.4 -D). At 20 days, all the leaf discs that were screwed, formed calluses.
    At 40 days, all the calli were individually weighed and the discs were transferred to a
    medium without 2,4-D and with different concentration of BAP to induce organogenesis.
    The regeneration frequency of callus was 60-80 percent. In the absence of 2,4-D, no
    calluses were formed. It is concluded that the first step of tissue culture of G. sepium is
    going in the right direction. Germ-free culture with high frequency and rapid growth of
    callus was obtained. The optimum ratio auxin: cytokinin was 2.5: 1. It is recommended to
    try different 2,4-D concentrations between 0 and 1 mg / l, to find response limit level to
    auxin. (CIAT).

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