Nikolaos Kaloumenos
NIKOLAOS S. KALOUMENOS
CURRICULUM VITAE
1. Personal Information
Date of birth: May 16th, 1975
Marital status: Single
Work Address: Syngenta
Jealott’s Hill International Research Centre,
Bracknell, Berkshire RG42 6EY, UK.
Email: [email protected]
Web pages: http://auth.academia.edu/NikolaosKaloumenos
http://www.researcherid.com/rid/F-3832-2011
2. Current appointment
• Research Project Biologist - Weed Control Biology. Syngenta, Jealott’s Hill International Research Centre. January 2012 up to date.
3. Past appointments
• Post-Doctoral Researcher. School of Agriculture, Division of Field Crops and Ecology, Aristotle University of Thessaloniki. January 2011 to December 2011
Research project funded by the Greek State Scholarship Foundation entitled ‘Study of the resistance mechanism and evaluation of the Actetolactate synthase activity of 13 resistant and one susceptible to chlorsulfuron ryegrass (Lolium rigidum) populations.
4. Education
4.1 Degrees
• Ph.D., School of Agriculture, Division of Field Crops and Ecology, Aristotle University of Thessaloniki, November 2009.
Title: ‘Resistance mechanism to herbicides, biology and chemical control of various corn poppy populations’.
Committee: Prof. Ilias Eleftherohorinos (Supervisor), Prof. Grigorios Diamantidis, and Prof. Panagiotis Efthimiadis.
• Master. School of Agriculture, Division of Field Crops and Ecology, Aristotle University of Thessaloniki, January 2003.
Title: ‘Residual activity, weed control, and selectivity of pyrithiobac in cotton’.
Supervisor: Prof. Ilias Eleftherohorinos.
• Bachelor, School of Agriculture, Division of Field Crops and Ecology, Aristotle University of Thessaloniki, July 2000.
Thesis: ‘Red rice (Oryza sativa) interference with rice (Oryza sativa) cultivars in paddy rice fields’
4.2 Grants and Fellowships
• Grand for Post-doctoral research by the Greek State Scholarships Foundation (February 2011 – February 2012).
• Excellence Grant (for concluding the M.Sc. studies in Aristotle University of Thessaloniki, school of agriculture, 2000-2002).
• Erasmus Grant (for concluding a semester in Naples University Federico II, faculty of agriculture, 1999-2000).
4.3 Other Qualifications and Skills
• Fluent in:
a. English (Proficiency, University of Cambridge).
b. Italian (C.E.L.I. 5, University of Perugia).
c. Spanish (Diploma Superior, Cervantes Institute).
d. Greek (Native speaker)
• Advanced computing skills in Windows and Macintosh interface.
• Qualified in statistics and statistic analysis of data with the use of the statistic programs MSTAT, SPSSX and Statistica 7.
5. Professional Experience
5.1 Teaching Experience
• Teaching Assistant, 2004 – 2010, Aristotle University of Thessaloniki, School of Agriculture.
Courses: Field and Lab Courses in Weed Science
• Teaching Assistant, 2004 – 2009, Technical Educational Institute of Thessaloniki, School of Agricultural Technology.
Courses: Field and Lab Courses in Weed Science
5.2 Research and Work Experience
• Research Agronomist, 2004-2005 in the research project ‘Netwet II’ funded by the «CENTRAL ADRIATIC DANUBIAN SOUTH-EASTERN EUROPEAN SPACE (CADSES)».
Supervisor: E. Anastasiadou-Partheniou (Aristotle University of
Thessaloniki, School of Agriculture, e-mail: [email protected]).
• Research Agronomist. 2001 in the research project ‘Evaluation of 25 corn hybrids in three locations’ funded by MONSANTO Hellas’.
Supervisor: I.G. Eleftherohorinos (Aristotle University of
Thessaloniki, School of Agriculture, e-mail: [email protected]).
• Occasional occupation in the family owned agricultural supplies trade company since its foundation (1988).
6. Research interests
• Characterization of new herbicide resistance cases
• Molecular characterization of herbicide resistance
• Weed biology and impact of resistance on weed fitness
• Weed – crop interference
• Evaluation of management programs for prevention or overcoming the herbicide resistance
• Field crop weed management
7. Publications
7.1 Dissertation
‘Resistance mechanism to herbicides, biology and chemical control of various corn poppy populations’. University Publications Service, Thessaloniki 2010.
Available on line from the Aristotle University of Thessaloniki library at:
http://invenio.lib.auth.gr/record/113748/files/Phd_Kaloumenos.pdf or
http://invenio.lib.auth.gr/record/113748/?ln=el
7.2 Published Research Articles
1. Kaloumenos, S.N., V.N. Adamouli, C.A. Dordas, and I.G. Eleftherohorinos. 2011. Corn Poppy (Papaver rhoeas) Cross-resistance to ALS-inhibiting Herbicides. Pest Management Science 67: 574-585.
2. Papapanagiotou P.A., N.S. Kaloumenos, and I.G. Eleftherohorinos. 2011. Sterile oat (Avena sterilis L.) cross-resistance profile to ACCase-inhibiting herbicides in Greece. Crop Protection (proof corrected returned on 18 August 2011 reference no JCRP2739).
3. Kaloumenos, N.S., C.A. Dordas, G.C. Diamantidis, and I.G. Eleftherochorinos. 2009.
Supervisors: Prof. Ilias Eleftherohorinos
CURRICULUM VITAE
1. Personal Information
Date of birth: May 16th, 1975
Marital status: Single
Work Address: Syngenta
Jealott’s Hill International Research Centre,
Bracknell, Berkshire RG42 6EY, UK.
Email: [email protected]
Web pages: http://auth.academia.edu/NikolaosKaloumenos
http://www.researcherid.com/rid/F-3832-2011
2. Current appointment
• Research Project Biologist - Weed Control Biology. Syngenta, Jealott’s Hill International Research Centre. January 2012 up to date.
3. Past appointments
• Post-Doctoral Researcher. School of Agriculture, Division of Field Crops and Ecology, Aristotle University of Thessaloniki. January 2011 to December 2011
Research project funded by the Greek State Scholarship Foundation entitled ‘Study of the resistance mechanism and evaluation of the Actetolactate synthase activity of 13 resistant and one susceptible to chlorsulfuron ryegrass (Lolium rigidum) populations.
4. Education
4.1 Degrees
• Ph.D., School of Agriculture, Division of Field Crops and Ecology, Aristotle University of Thessaloniki, November 2009.
Title: ‘Resistance mechanism to herbicides, biology and chemical control of various corn poppy populations’.
Committee: Prof. Ilias Eleftherohorinos (Supervisor), Prof. Grigorios Diamantidis, and Prof. Panagiotis Efthimiadis.
• Master. School of Agriculture, Division of Field Crops and Ecology, Aristotle University of Thessaloniki, January 2003.
Title: ‘Residual activity, weed control, and selectivity of pyrithiobac in cotton’.
Supervisor: Prof. Ilias Eleftherohorinos.
• Bachelor, School of Agriculture, Division of Field Crops and Ecology, Aristotle University of Thessaloniki, July 2000.
Thesis: ‘Red rice (Oryza sativa) interference with rice (Oryza sativa) cultivars in paddy rice fields’
4.2 Grants and Fellowships
• Grand for Post-doctoral research by the Greek State Scholarships Foundation (February 2011 – February 2012).
• Excellence Grant (for concluding the M.Sc. studies in Aristotle University of Thessaloniki, school of agriculture, 2000-2002).
• Erasmus Grant (for concluding a semester in Naples University Federico II, faculty of agriculture, 1999-2000).
4.3 Other Qualifications and Skills
• Fluent in:
a. English (Proficiency, University of Cambridge).
b. Italian (C.E.L.I. 5, University of Perugia).
c. Spanish (Diploma Superior, Cervantes Institute).
d. Greek (Native speaker)
• Advanced computing skills in Windows and Macintosh interface.
• Qualified in statistics and statistic analysis of data with the use of the statistic programs MSTAT, SPSSX and Statistica 7.
5. Professional Experience
5.1 Teaching Experience
• Teaching Assistant, 2004 – 2010, Aristotle University of Thessaloniki, School of Agriculture.
Courses: Field and Lab Courses in Weed Science
• Teaching Assistant, 2004 – 2009, Technical Educational Institute of Thessaloniki, School of Agricultural Technology.
Courses: Field and Lab Courses in Weed Science
5.2 Research and Work Experience
• Research Agronomist, 2004-2005 in the research project ‘Netwet II’ funded by the «CENTRAL ADRIATIC DANUBIAN SOUTH-EASTERN EUROPEAN SPACE (CADSES)».
Supervisor: E. Anastasiadou-Partheniou (Aristotle University of
Thessaloniki, School of Agriculture, e-mail: [email protected]).
• Research Agronomist. 2001 in the research project ‘Evaluation of 25 corn hybrids in three locations’ funded by MONSANTO Hellas’.
Supervisor: I.G. Eleftherohorinos (Aristotle University of
Thessaloniki, School of Agriculture, e-mail: [email protected]).
• Occasional occupation in the family owned agricultural supplies trade company since its foundation (1988).
6. Research interests
• Characterization of new herbicide resistance cases
• Molecular characterization of herbicide resistance
• Weed biology and impact of resistance on weed fitness
• Weed – crop interference
• Evaluation of management programs for prevention or overcoming the herbicide resistance
• Field crop weed management
7. Publications
7.1 Dissertation
‘Resistance mechanism to herbicides, biology and chemical control of various corn poppy populations’. University Publications Service, Thessaloniki 2010.
Available on line from the Aristotle University of Thessaloniki library at:
http://invenio.lib.auth.gr/record/113748/files/Phd_Kaloumenos.pdf or
http://invenio.lib.auth.gr/record/113748/?ln=el
7.2 Published Research Articles
1. Kaloumenos, S.N., V.N. Adamouli, C.A. Dordas, and I.G. Eleftherohorinos. 2011. Corn Poppy (Papaver rhoeas) Cross-resistance to ALS-inhibiting Herbicides. Pest Management Science 67: 574-585.
2. Papapanagiotou P.A., N.S. Kaloumenos, and I.G. Eleftherohorinos. 2011. Sterile oat (Avena sterilis L.) cross-resistance profile to ACCase-inhibiting herbicides in Greece. Crop Protection (proof corrected returned on 18 August 2011 reference no JCRP2739).
3. Kaloumenos, N.S., C.A. Dordas, G.C. Diamantidis, and I.G. Eleftherochorinos. 2009.
Supervisors: Prof. Ilias Eleftherohorinos
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Papers by Nikolaos Kaloumenos
10 putative resistant (R) Cyperus difformis (smallflower umbrella-sedge) populations collected from rice fields
located in Northern Greece. However, the growth rate of the susceptible (S) population was reduced by 50%
by 1/4 of the recommended rate of both herbicides. The als gene sequencing identified point mutations at Pro-
197 position, leading to amino acid substitution by Ala or Ser of the ALS enzyme. The in vitro activity of the ALS
enzyme indicated that the I50 values (herbicide concentration required for 50% reduction of the ALS activity)
ranged from10.7 to 55.5 μMazimsulfuron and from 6.7 to 50.6 μMhalosulfuron-methyl, whereas the respective
values for the S population were 0.09 and 0.11 μM. These results strongly support that cross-resistance of 10
C. difformis populationswas due to point mutations of the als gene,which resulted in a less-sensitive ALS enzyme.
This study reports the presence of a 1240 bp intron in the C. difformis als gene with the Pro-197 point mutations
near the splice junction.
RESULTS: The whole-plant trials revealed that tribenuron R populations were also cross-resistant to sulfonylureas mesosulfuron + iodosulfuron, chlorsulfuron and triasulfuron. The whole-plant resistance factors (RFs) calculated for pyrithiobac, imazamox and florasulam ranged from 12.4 to >88, from 1.5 to 28.3 and from 5.6 to 25.4, respectively, and were lower than the respective tribenuron RF values (137 to >2400). The ALS activity assay showed higher resistance of the ALS enzyme to sulfonylurea herbicides (tribenuron > chlorsulfuron) and lower resistance to non-sulfonylurea ALS-inhibiting herbicides (pyrithiobac > florasulam ≈ imazamox).
CONCLUSION: These findings indicate that Pro197 substitution by Ala, Ser, Arg or Thr in corn poppy results in a less sensitive ALS enzyme to sulfonylurea herbicides than to other ALS-inhibiting herbicides. The continued use of sulfonylurea herbicides led to cross-resistance to all ALS-inhibiting herbicides, making their use impossible in corn poppy resistance management programmes.
10 putative resistant (R) Cyperus difformis (smallflower umbrella-sedge) populations collected from rice fields
located in Northern Greece. However, the growth rate of the susceptible (S) population was reduced by 50%
by 1/4 of the recommended rate of both herbicides. The als gene sequencing identified point mutations at Pro-
197 position, leading to amino acid substitution by Ala or Ser of the ALS enzyme. The in vitro activity of the ALS
enzyme indicated that the I50 values (herbicide concentration required for 50% reduction of the ALS activity)
ranged from10.7 to 55.5 μMazimsulfuron and from 6.7 to 50.6 μMhalosulfuron-methyl, whereas the respective
values for the S population were 0.09 and 0.11 μM. These results strongly support that cross-resistance of 10
C. difformis populationswas due to point mutations of the als gene,which resulted in a less-sensitive ALS enzyme.
This study reports the presence of a 1240 bp intron in the C. difformis als gene with the Pro-197 point mutations
near the splice junction.
RESULTS: The whole-plant trials revealed that tribenuron R populations were also cross-resistant to sulfonylureas mesosulfuron + iodosulfuron, chlorsulfuron and triasulfuron. The whole-plant resistance factors (RFs) calculated for pyrithiobac, imazamox and florasulam ranged from 12.4 to >88, from 1.5 to 28.3 and from 5.6 to 25.4, respectively, and were lower than the respective tribenuron RF values (137 to >2400). The ALS activity assay showed higher resistance of the ALS enzyme to sulfonylurea herbicides (tribenuron > chlorsulfuron) and lower resistance to non-sulfonylurea ALS-inhibiting herbicides (pyrithiobac > florasulam ≈ imazamox).
CONCLUSION: These findings indicate that Pro197 substitution by Ala, Ser, Arg or Thr in corn poppy results in a less sensitive ALS enzyme to sulfonylurea herbicides than to other ALS-inhibiting herbicides. The continued use of sulfonylurea herbicides led to cross-resistance to all ALS-inhibiting herbicides, making their use impossible in corn poppy resistance management programmes.