Studying structure and function of membrane proteins with PELDOR/DEER spectroscopy - A crystallographers' perspective. (Q52671619)

5 May 2018

title

Studying structure and function of membrane proteins with PELDOR/DEER spectroscopy - A crystallographers' perspective. (English)

1 reference

5 May 2018

1 reference

Janin Glaenzer

1

1 reference

5 May 2018

Martin F Peter

series ordinal

2

1 reference

5 May 2018

Gregor Hagelueken

series ordinal

3

1 reference

5 May 2018

publication date

3 March 2018

1 reference

5 May 2018

published in

Methods

1 reference

5 May 2018

Prediction of the human membrane proteome.

cites work

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Overcoming the challenges of membrane protein crystallography.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3Å resolution

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

The revolution will not be crystallized: a new method sweeps through structural biology

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Crystallizing membrane proteins for structure determination: use of lipidic mesophases

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Dead-Time Free Measurement of Dipole–Dipole Interactions between Electron Spins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

DEER distance measurements on proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Long-range distance determinations in biomacromolecules by EPR spectroscopy

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Navigating Membrane Protein Structure, Dynamics, and Energy Landscapes Using Spin Labeling and EPR Spectroscopy.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Pulsed EPR spectroscopy: biological applications

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

New pulsed EPR methods and their application to characterize mitochondrial complex I.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Photosynthesis. Electronic structure of the oxygen-evolving complex in photosystem II prior to O-O bond formation

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Distance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Transmembrane protein structure: spin labeling of bacteriorhodopsin mutants

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

A novel reversible thiol-specific spin label: Papain active site labeling and inhibition

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Technological advances in site-directed spin labeling of proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Site-directed spin labeling of a genetically encoded unnatural amino acid

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Distance measurements in model bis-Gd(III) complexes with flexible "bridge". Emulation of biological molecules having flexible structure with Gd(III) labels attached.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Pulsed dipolar spectroscopy distance measurements in biomacromolecules labeled with Gd(III) markers

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Trityl radicals: spin labels for nanometer-distance measurements

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Versatile Trityl Spin Labels for Nanometer Distance Measurements on Biomolecules In Vitro and within Cells

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Rigid spin-labeled nucleoside C: a nonperturbing EPR probe of nucleic acid conformation.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Base-specific spin-labeling of RNA for structure determination

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Site-specific incorporation of nitroxide spin-labels into internal sites of the TAR RNA; structure-dependent dynamics of RNA by EPR spectroscopy

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Quantitative analysis of the isolated GAAA tetraloop/receptor interaction in solution: a site-directed spin labeling study

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

A practical guide to single-molecule FRET

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Simulation vs. reality: a comparison of in silico distance predictions with DEER and FRET measurements

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Tripartite ATP-independent periplasmic (TRAP) transporters in bacteria and archaea

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Bacterial periplasmic sialic acid-binding proteins exhibit a conserved binding site

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Conservation of structure and mechanism in primary and secondary transporters exemplified by SiaP, a sialic acid binding virulence factor from Haemophilus influenzae

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Characterization of the N-acetyl-5-neuraminic acid-binding site of the extracytoplasmic solute receptor (SiaP) of nontypeable Haemophilus influenzae strain 2019

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

PELDOR Spectroscopy Reveals Two Defined States of a Sialic Acid TRAP Transporter SBP in Solution

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Computer modeling of nitroxide spin labels on proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

MtsslWizard: In Silico Spin-Labeling and Generation of Distance Distributions in PyMOL.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

mtsslSuite: Probing Biomolecular Conformation by Spin-Labeling Studies

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

High-resolution crystal structure of spin labelled (T21R1) azurin from Pseudomonas aeruginosa: a challenging structural benchmark for in silico spin labelling algorithms

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Conformational dynamics and distribution of nitroxide spin labels

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

RosettaEPR: rotamer library for spin label structure and dynamics

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Domain identification by iterative analysis of error-scaled difference distance matrices

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Adding new chemistries to the genetic code

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

A genetically encoded spin label for electron paramagnetic resonance distance measurements

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Ketoxime coupling of p-acetylphenylalanine at neutral pH for site-directed spin labeling of human sulfite oxidase.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Orthogonal spin labeling using click chemistry for in vitro and in vivo applications

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Expression, purification and spin labelling of the ferrous iron transporter FeoB from Escherichia coli BL21 for EPR studies

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Assessing oligomerization of membrane proteins by four-pulse DEER: pH-dependent dimerization of NhaA Na+/H+ antiporter of E. coli.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Distance determination in proteins inside Xenopus laevis oocytes by double electron-electron resonance experiments.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Long-range distance measurements on nucleic acids in cells by pulsed EPR spectroscopy

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Determination of the distance between two spin labels attached to a macromolecule

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

High sensitivity and versatility of the DEER experiment on nitroxide radical pairs at Q-band frequencies.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Significantly improved sensitivity of Q-band PELDOR/DEER experiments relative to X-band is observed in measuring the intercoil distance of a leucine zipper motif peptide (GCN4-LZ)

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Size and shape of protein molecules at the nanometer level determined by sedimentation, gel filtration, and electron microscopy.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Substrate-dependent unfolding of the energy coupling motif of a membrane transport protein determined by double electron-electron resonance

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Counting the monomers in nanometer-sized oligomers by pulsed electron-electron double resonance

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Studies on the X-Ray and Solution Structure of FeoB from Escherichia coli BL21.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Direct conversion of EPR dipolar time evolution data to distance distributions

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

The determination of pair distance distributions by pulsed ESR using Tikhonov regularization.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

DeerAnalysis2006—a comprehensive software package for analyzing pulsed ELDOR data

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Optimization of transversal relaxation of nitroxides for pulsed electron-electron double resonance spectroscopy in phospholipid membranes

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Directed self-assembly of monodisperse phospholipid bilayer Nanodiscs with controlled size

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

EPR distance measurements in deuterated proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

A Bayesian approach to quantifying uncertainty from experimental noise in DEER spectroscopy

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Optimal Tikhonov regularization for DEER spectroscopy

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Bacterial mechanosensitive channels--MscS: evolution's solution to creating sensitivity in function

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Probing the structure of the mechanosensitive channel of small conductance in lipid bilayers with pulsed electron-electron double resonance.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

The role of lipids in mechanosensation

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Energy transduction and alternating access of the mammalian ABC transporter P-glycoprotein

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Refined structures of mouse P-glycoprotein

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Crystal structure of the multidrug transporter P-glycoprotein from Caenorhabditis elegans

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Wza the translocon for E. coli capsular polysaccharides defines a new class of membrane protein

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

PELDOR Spectroscopy Distance Fingerprinting of the Octameric Outer-Membrane Protein Wza from Escherichia coli

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

An allosteric mechanism for activation of the kinase domain of epidermal growth factor receptor

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Mechanism for activation of the EGF receptor catalytic domain by the juxtamembrane segment

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Studying the Conformation of a Receptor Tyrosine Kinase in Solution by Inhibitor-Based Spin Labeling

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Ion/substrate-dependent conformational dynamics of a bacterial homolog of neurotransmitter:sodium symporters.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

EPR-based approach for the localization of paramagnetic metal ions in biomolecules

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Locating a lipid at the portal to the lipoxygenase active site

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

A novel microfluidic rapid freeze-quench device for trapping reactions intermediates for high field EPR analysis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Rates and equilibrium constants of the ligand-induced conformational transition of an HCN ion channel protein domain determined by DEER spectroscopy

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Chapter 4 Electron transfer in respiratory complexes resolved by an ultra-fast freeze-quench approach.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

Refolding of the integral membrane protein light-harvesting complex II monitored by pulse EPR.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

EPR-based distance measurements at ambient temperature.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2018.03.002

10.1016/J.YMETH.2018.03.002

7 January 2021

Identifiers

DOI

1 reference

5 May 2018

1 reference

5 May 2018