A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding (Q34990932)

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21 February 2020

title

A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding (English)

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21 February 2020

6

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21 February 2020

author name string

Marie-Ève Lacombe-Harvey

1

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21 February 2020

Mélanie Fortin

2

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21 February 2020

Takayuki Ohnuma

3

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21 February 2020

Tamo Fukamizo

4

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21 February 2020

Thomas Letzel

5

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21 February 2020

publication date

16 September 2013

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21 February 2020

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21 February 2020

volume

14

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21 February 2020

page(s)

23

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Degradation of Chitosans with a Family 46 Chitosanase from Streptomyces coelicolor A3(2)

21 February 2020

cites work

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26kDa endochitinase from barley seeds: real-time monitoring of the enzymatic reaction and substrate binding experiments using electrospray ionization mass spectrometry

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Role of acidic amino acid residues in chitooligosaccharide-binding to Streptomyces sp. N174 chitosanase

8 July 2018

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Chitinolytic enzymes: catalysis, substrate binding, and their application

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Mechanism of chitosanase-oligosaccharide interaction: subsite structure of Streptomyces sp. N174 chitosanase and the role of Asp57 carboxylate

8 July 2018

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Enzyme catalysis: removing chemically 'essential' residues by site-directed mutagenesis.

8 July 2018

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Molecular cloning and characterization of a chitosanase from the chitosanolytic bacterium Burkholderia gladioli strain CHB101.

8 July 2018

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8 July 2018

Glycosidase mechanisms

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Mutagenesis of glycosidases

8 July 2018

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Crystal structure of chitosanase from Bacillus circulans MH-K1 at 1.6-A resolution and its substrate recognition mechanism

8 July 2018

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Thermal unfolding of chitosanase from Streptomyces sp. N174: role of tryptophan residues in the protein structure stabilization

8 July 2018

1 reference

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Positioning hydrogen atoms by optimizing hydrogen-bond networks in protein structures

8 July 2018

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X-ray structure of an anti-fungal chitosanase from streptomyces N174

8 July 2018

1 reference

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Chitinases, chitosanases, and lysozymes can be divided into procaryotic and eucaryotic families sharing a conserved core.

8 July 2018

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Mutational and crystallographic analyses of the active site residues of the Bacillus circulans xylanase

8 July 2018

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Reaction mechanism of chitosanase from Streptomyces sp. N174.

8 July 2018

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A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments

8 July 2018

2 references

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8 July 2018

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Tinkering with enzymes: what are we learning?

21 January 2018

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Site-directed mutagenesis by overlap extension using the polymerase chain reaction

8 July 2018

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WHAT IF: a molecular modeling and drug design program

8 July 2018

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PROSITE: a dictionary of sites and patterns in proteins

8 July 2018

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A versatile shuttle cosmid vector for use in Escherichia coli and actinomycetes.

8 July 2018

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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

8 July 2018

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Site-directed mutagenesis of evolutionary conserved carboxylic amino acids in the chitosanase from Streptomyces sp. N174 reveals two residues essential for catalysis

8 July 2018

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Accessory active site residues of Streptomyces sp. N174 chitosanase: variations on a common theme in the lysozyme superfamily

21 January 2018

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A validated 1H NMR method for the determination of the degree of deacetylation of chitosan

21 January 2018

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Biochemical and molecular characterization of a thermostable chitosanase produced by the strain Paenibacillus sp. 1794 newly isolated from compost

21 January 2018

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BIOCHEMISTRY: How Enzymes Work

21 January 2018

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tdd8: a TerD domain-encoding gene involved in Streptomyces coelicolor differentiation

21 January 2018

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Characterization and kinetics of 45 kDa chitosanase from Bacillus sp. P16.

26 October 2018

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Effect of the degree of acetylation of chitosan on its enzymatic hydrolysis with the preparation Celloviridin G20x

26 October 2018

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Substrate inhibition kinetics for cytochrome P450-catalyzed reactions

26 October 2018

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A common molecular signature unifies the chitosanases belonging to families 46 and 80 of glycoside hydrolases

26 October 2018

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Theoretical calculation of pKa reveals an important role of Arg205 in the activity and stability of Streptomyces sp. N174 chitosanase

26 October 2018

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12 December 2020

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12 December 2020

Chitosanase-catalyzed hydrolysis of 4-methylumbelliferyl beta-chitotrioside

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12 December 2020

Mass spectrometric real-time monitoring of enzymatic glycosidic hydrolysis, enzymatic inhibition and enzyme complexes

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12 December 2020

Quantitative fluorometric analysis of the protective effect of chitosan on thermal unfolding of catalytically active native and genetically-engineered chitosanases

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12 December 2020

Oligosaccharide hydrolysis by chitosanase enzymes monitored by real-time electrospray ionization-mass spectrometry

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12 December 2020

Identifiers

DOI

10.1186/1471-2091-14-23

1 reference

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21 February 2020

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21 February 2020

PubMed publication ID

24041306

1 reference

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