Large-scale de novo DNA synthesis: technologies and applications (Q34417984)

2 August 2017

title

Large-scale de novo DNA synthesis: technologies and applications (English)

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2 August 2017

Sriram Kosuri

1

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George M. Church

object named as

George M Church

series ordinal

2

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publication date

1 May 2014

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2 August 2017

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2 August 2017

volume

11

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2 August 2017

issue

5

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2 August 2017

page(s)

499-507

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The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides

2 August 2017

cites work

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

The rise of a digital immune system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

The expanding scope of DNA sequencing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Synthesis of DNA/RNA and their analogs via phosphoramidite and H-phosphonate chemistries

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

644. Nucleotides. Part XLI. Mixed anhydrides as intermediates in the synthesis of dinucleoside phosphates

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Kinetic studies on depurination and detritylation of CPG-bound intermediates during oligonucleotide synthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Correcting errors in synthetic DNA through consensus shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Alpha1-induced DNA bending is required for transcriptional activation by the Mcm1-alpha1 complex

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Light-directed, spatially addressable parallel chemical synthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Light-generated oligonucleotide arrays for rapid DNA sequence analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

A flexible light-directed DNA chip synthesis gated by deprotection using solution photogenerated acids

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

High-density oligonucleotide arrays

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

CombiMatrix oligonucleotide arrays: genotyping and gene expression assays employing electrochemical detection

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Gene synthesis by a LCR-based approach: high-level production of leptin-L54 using synthetic gene in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Gene synthesis by circular assembly amplification

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Oligonucleotide assembly in yeast to produce synthetic DNA fragments

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Synthetic generation of influenza vaccine viruses for rapid response to pandemics

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Chemical synthesis of the mouse mitochondrial genome.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Gene synthesis demystified

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Chemical gene synthesis: strategies, softwares, error corrections, and applications

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Accurate multiplex gene synthesis from programmable DNA microchips

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Microfluidic PicoArray synthesis of oligodeoxynucleotides and simultaneous assembling of multiple DNA sequences

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Amplification and assembly of chip-eluted DNA (AACED): a method for high-throughput gene synthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

High-quality gene assembly directly from unpurified mixtures of microarray-synthesized oligonucleotides

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Parallel on-chip gene synthesis and application to optimization of protein expression

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

'Shotgun DNA synthesis' for the high-throughput construction of large DNA molecules

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

A Fluorescence Selection Method for Accurate Large‐Gene Synthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Multifactorial Determinants of Protein Expression in Prokaryotic Open Reading Frames

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Removal of polymerase-produced mutant sequences from PCR products

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Two-step total gene synthesis method

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Probing the limits of genetic recoding in essential genes.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Programming biological operating systems: genome design, assembly and activation

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Enzymatic assembly of DNA molecules up to several hundred kilobases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

SLiCE: a novel bacterial cell extract-based DNA cloning method

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Assembling large DNA segments in yeast.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Circular polymerase extension cloning of complex gene libraries and pathways

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

A Modular Cloning System for Standardized Assembly of Multigene Constructs

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Production of complex nucleic acid libraries using highly parallel in situ oligonucleotide synthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Enrichment of sequencing targets from the human genome by solution hybridization

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Multiplex amplification of large sets of human exons

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Specific capture and whole-genome sequencing of viruses from clinical samples

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Targeted genome enrichment for efficient purification of endosymbiont DNA from host DNA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Multiplex padlock targeted sequencing reveals human hypermutable CpG variations

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Genome-wide identification of human RNA editing sites by parallel DNA capturing and sequencing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Digital RNA allelotyping reveals tissue-specific and allele-specific gene expression in human

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Autoantigen discovery with a synthetic human peptidome

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

PhIP-Seq characterization of autoantibodies from patients with multiple sclerosis, type 1 diabetes and rheumatoid arthritis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Construction of a rationally designed antibody platform for sequencing-assisted selection

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Genome-scale CRISPR-Cas9 knockout screening in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Genetic screens in human cells using the CRISPR-Cas9 system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Synthetic design of strong promoters

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Causes and effects of N-terminal codon bias in bacterial genes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Composability of regulatory sequences controlling transcription and translation in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Systematic dissection and optimization of inducible enhancers in human cells using a massively parallel reporter assay

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Systematic dissection of regulatory motifs in 2000 predicted human enhancers using a massively parallel reporter assay

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Complex effects of nucleotide variants in a mammalian cis-regulatory element.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Massively parallel in vivo enhancer assay reveals that highly local features determine the cis-regulatory function of ChIP-seq peaks.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Massively parallel synthetic promoter assays reveal the in vivo effects of binding site variants

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

The DNA-encoded nucleosome organization of a eukaryotic genome

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Inferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Massively parallel decoding of mammalian regulatory sequences supports a flexible organizational model

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Synthesis of methyl halides from biomass using engineered microbes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Coding-sequence determinants of gene expression in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Deep mutational scanning: assessing protein function on a massive scale

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Deep mutational scanning of an RRM domain of the Saccharomyces cerevisiae poly(A)-binding protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

High-resolution mapping of protein sequence-function relationships

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

High-throughput analysis of in vivo protein stability

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

The spatial architecture of protein function and adaptation

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Hot spots for allosteric regulation on protein surfaces

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Refactoring bacteriophage T7

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

A fully decompressed synthetic bacteriophage øX174 genome assembled and archived in yeast

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Refactored M13 bacteriophage as a platform for tumor cell imaging and drug delivery

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Measurement and modeling of intrinsic transcription terminators

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Characterization of 582 natural and synthetic terminators and quantification of their design constraints

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Precise and reliable gene expression via standard transcription and translation initiation elements

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Quantitative estimation of activity and quality for collections of functional genetic elements

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Genomic mining of prokaryotic repressors for orthogonal logic gates

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Design of orthogonal genetic switches based on a crosstalk map of σs, anti-σs, and promoters

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Characterization of the reconstructed 1918 Spanish influenza pandemic virus

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Synthetic recombinant bat SARS-like coronavirus is infectious in cultured cells and in mice

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Generation of infectious molecular clones of simian immunodeficiency virus from fecal consensus sequences of wild chimpanzees

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Genetic inactivation of poliovirus infectivity by increasing the frequencies of CpG and UpA dinucleotides within and across synonymous capsid region codons.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Identification of an infectious progenitor for the multiple-copy HERV-K human endogenous retroelements

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

An inactivated West Nile Virus vaccine derived from a chemically synthesized cDNA system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Live attenuated influenza virus vaccines by computer-aided rational design

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Modulation of poliovirus replicative fitness in HeLa cells by deoptimization of synonymous codon usage in the capsid region.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Reconstitution of an infectious human endogenous retrovirus

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Reduction of the rate of poliovirus protein synthesis through large-scale codon deoptimization causes attenuation of viral virulence by lowering specific infectivity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Synthetic poliovirus and other designer viruses: what have we learned from them?

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Virus attenuation by genome-scale changes in codon pair bias

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Whole-genome synthesis and characterization of viable S13-like bacteriophages

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Synthetic chromosome arms function in yeast and generate phenotypic diversity by design

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Folding DNA to create nanoscale shapes and patterns

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Folding DNA into twisted and curved nanoscale shapes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Self-assembly of DNA into nanoscale three-dimensional shapes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Three-dimensional structures self-assembled from DNA bricks

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Self-assembly of carbon nanotubes into two-dimensional geometries using DNA origami templates

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

A logic-gated nanorobot for targeted transport of molecular payloads

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

DNA as a universal substrate for chemical kinetics

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Scaling up digital circuit computation with DNA strand displacement cascades

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

CCL2 recruits inflammatory monocytes to facilitate breast-tumour metastasis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Next-generation digital information storage in DNA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Towards practical, high-capacity, low-maintenance information storage in synthesized DNA

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

One-pot assembly of a hetero-dimeric DNA origami from chip-derived staples and double-stranded scaffold.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Error correction of microchip synthesized genes using Surveyor nuclease

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from Yeast

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

An autonomous polymerization motor powered by DNA hybridization

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Neural network computation with DNA strand displacement cascades

21 January 2018

1 reference

12 December 2020

Total synthesis of a tyrosine suppressor transfer RNA gene. XVI. Enzymatic joinings to form the total 207-base pair-long DNA

1 reference

12 December 2020

Rapid and reliable DNA assembly via ligase cycling reaction

1 reference

12 December 2020

In situ Synthesis of DNA Microarray on Functionalized Cyclic Olefin Copolymer Substrate

1 reference

12 December 2020

When it rains, it pores

1 reference

12 December 2020

Principles of genetic circuit design

1 reference

12 December 2020

Protein-mediated error correction for de novo DNA synthesis

1 reference

12 December 2020

Nucleotides part XXXII. Synthesis of a dithymidine dinucleotide containing a 3′: 5′-internucleotidic linkage

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

7 January 2021

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Identifiers

DOI

10.1038/NMETH.2918

1 reference

Dimensions Publication ID

2 August 2017

1022164954

0 references

1 reference