Semiquantitative proteomic analysis of the human spliceosome via a novel two-dimensional gel electrophoresis method (Q34181884)

31 July 2017

title

Semiquantitative proteomic analysis of the human spliceosome via a novel two-dimensional gel electrophoresis method (English)

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31 July 2017

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Elmar Wolf

3

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Reinhard Georg Lührmann

8

object named as

Reinhard Lührmann

1 reference

31 July 2017

6

Cindy L Will

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31 July 2017

7

Henning Urlaub

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31 July 2017

Dmitry E Agafonov

1

1 reference

31 July 2017

Jochen Deckert

2

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31 July 2017

Peter Odenwälder

4

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31 July 2017

Sergey Bessonov

5

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31 July 2017

language of work or name

English

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publication date

2 May 2011

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Molecular and Cellular Biology

31 July 2017

published in

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31 July 2017

volume

31

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31 July 2017

issue

13

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31 July 2017

page(s)

2667-2682

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Functional organization of the Sm core in the crystal structure of human U1 snRNP

31 July 2017

cites work

1 reference

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Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis

5 July 2018

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Determination of protein stoichiometry within protein complexes using absolute quantification and multiple reaction monitoring

5 July 2018

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Molecular architecture of the human Prp19/CDC5L complex

5 July 2018

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The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome

5 July 2018

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Cell cycle-dependent phosphorylation of human CDC5 regulates RNA processing.

5 July 2018

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Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions

5 July 2018

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Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein

5 July 2018

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Protein stoichiometry of a multiprotein complex, the human spliceosomal U1 small nuclear ribonucleoprotein: absolute quantification using isotope-coded tags and mass spectrometry

5 July 2018

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Large-scale characterization of HeLa cell nuclear phosphoproteins

5 July 2018

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A subset of human 35S U5 proteins, including Prp19, function prior to catalytic step 1 of splicing

5 July 2018

1 reference

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The Prp19p-associated complex in spliceosome activation

5 July 2018

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Global quantitative phosphoprotein analysis using Multiplexed Proteomics technology

5 July 2018

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Phosphorylation-dependent control of the pre-mRNA splicing machinery

5 July 2018

1 reference

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Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein

5 July 2018

1 reference

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Comprehensive proteomic analysis of the human spliceosome

5 July 2018

1 reference

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Large-scale proteomic analysis of the human spliceosome

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

Background-free, high sensitivity staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels using a luminescent ruthenium complex

5 July 2018

1 reference

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Purification of SR protein splicing factors

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

A protein residing at the subunit interface of the bacterial ribosome

5 July 2018

1 reference

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RNA splicing: What has phosphorylation got to do with it?

5 July 2018

1 reference

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Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif

5 July 2018

1 reference

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Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro

5 July 2018

1 reference

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The [U4/U6.U5] tri-snRNP-specific 27K protein is a novel SR protein that can be phosphorylated by the snRNP-associated protein kinase

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels

5 July 2018

1 reference

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A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

5 July 2018

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Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis

5 July 2018

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Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

5 July 2018

1 reference

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Characterisation of human and murine snRNP proteins by two-dimensional gel electrophoresis and phosphopeptide analysis of U1-specific 70K protein variants

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

Protein components specifically associated with prespliceosome and spliceosome complexes

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

A new two-dimensional gel electrophoresis system for the analysis of complex protein mixtures: Application to the ribosome of E. coli

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

High resolution two-dimensional electrophoresis of proteins

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

3D cryo-EM structure of an active step I spliceosome and localization of its catalytic core

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

Human PRP4 kinase is required for stable tri-snRNP association during spliceosomal B complex formation

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

Isolation of an active step I spliceosome and composition of its RNP core

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

Photopolymerization of polyacrylamide gels with methylene blue

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

On the limiting pore size of hydrophilic gels for electrophoresis and isoelectric focusing

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3133382

Purification of U small nuclear ribonucleoprotein particles

28 October 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/21536652

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Thiophosphorylation of U1-70K protein inhibits pre-mRNA splicing

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/21536652

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

A functional association between the 5' and 3' splice site is established in the earliest prespliceosome complex (E) in mammals

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/21536652

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1128/MCB.05266-11

1 reference

31 July 2017

1 reference

31 July 2017

1 reference