Recombinant protein expression in Pichia pastoris (Q33926665)

30 July 2017

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title

Recombinant protein expression in Pichia pastoris (English)

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30 July 2017

Pichia pastoris

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Cregg JM

1

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30 July 2017

Cereghino JL

series ordinal

2

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30 July 2017

Shi J

series ordinal

3

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30 July 2017

Higgins DR

series ordinal

4

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30 July 2017

publication date

1 September 2000

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30 July 2017

published in

Molecular Biotechnology

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30 July 2017

volume

16

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30 July 2017

issue

1

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30 July 2017

page(s)

23-52

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Functional expression of bovine opsin in the methylotrophic yeast Pichia pastoris

30 July 2017

cites work

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12 December 2020

Sequence, S-S bridges, and spectra of bovine transcobalamin expressed in Pichia pastoris

1 reference

12 December 2020

Large‐scale Generation of Affinity‐purified Recombinant Phytochrome Chromopeptide

1 reference

12 December 2020

High-level expression of cockroach allergen, Bla g 4, in Pichia pastoris.

1 reference

12 December 2020

Expression, purification, and characterization of interferon-tau produced in Pichia pastoris grown in a minimal medium

1 reference

12 December 2020

Glycosylation of human alpha 1-antitrypsin in Saccharomyces cerevisiae and methylotrophic yeasts

1 reference

12 December 2020

Foreign gene expression in yeast: a review

1 reference

12 December 2020

Evidence for the formation of a heterotrimeric complex of leukaemia inhibitory factor with its receptor subunits in solution

1 reference

12 December 2020

Enhancement through mutagenesis of the binding of the isolated kringle 2 domain of human plasminogen to omega-amino acid ligands and to an internal sequence of a Streptococcal surface protein

1 reference

12 December 2020

A group of alpha-1,4-glucan lyases and their genes from the red alga Gracilariopsis lemaneiformis: purification, cloning, and heterologous expression

1 reference

12 December 2020

Complete assignment of 1H, 13C and 15N chemical shifts for bovine beta-lactoglobulin: secondary structure and topology of the native state is retained in a partially unfolded form

1 reference

12 December 2020

Human cathepsin X: A cysteine protease with unique carboxypeptidase activity

1 reference

12 December 2020

Functional expression of Rhizopus oryzae lipase in Pichia pastoris: high-level production and some properties

1 reference

12 December 2020

Cloning and disruption of the antigenic catalase gene of Aspergillus fumigatus

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12 December 2020

Recombinant human mast cell tryptase beta: stable expression in Pichia pastoris and purification of fully active enzyme

1 reference

12 December 2020

Synthesis of amidrazones using an engineered papain nitrile hydratase

1 reference

12 December 2020

Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences

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12 December 2020

Development of the methylotrophic yeastPichia methanolica for the expression of the 65 kilodalton isoform of human glutamate decarboxylase

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12 December 2020

Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins

1 reference

12 December 2020

Recombinant type A and B phytochromes from potato. Transient absorption spectroscopy

1 reference

12 December 2020

In vitro production of recombinant antibody fragments in Pichia pastoris

1 reference

12 December 2020

Expression and alteration of the S2 subsite of the Leishmania major cathepsin B-like cysteine protease

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12 December 2020

NMR analysis of the N-terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments

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12 December 2020

Structural comparison of the Pichia pastoris alcohol oxidase genes

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12 December 2020

X-ray crystallography and mass spectroscopy reveal that the N-lobe of human transferrin expressed in Pichia pastoris is folded correctly but is glycosylated on serine-32

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12 December 2020

Application of yeasts in gene expression studies: a comparison of Saccharomyces cerevisiae, Hansenula polymorpha and Kluyveromyces lactis -- a review

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12 December 2020

Secretion of human intracellular aspartic proteinase cathepsin E expressed in the methylotrophic yeast, Pichia pastoris and characterization of produced recombinant cathepsin E

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12 December 2020

Drosophila melanogaster angiotensin I-converting enzyme expressed in Pichia pastoris resembles the C domain of the mammalian homologue and does not require glycosylation for secretion and enzymic activity

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12 December 2020

Functional Studies of Yeast-Expressed Human Heart Muscle Carnitine Palmitoyltransferase I

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12 December 2020

Production and isolation of the recombinant N-lobe of human serum transferrin from the methylotrophic yeast Pichia pastoris

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12 December 2020

Overexpression in Pichia pastoris and crystallization of an elicitor protein secreted by the phytopathogenic fungus, Phytophthora cryptogea

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12 December 2020

Role of tryptophan-63 of the kringle 2 domain of tissue-type plasminogen activator in its thermal stability, folding, and ligand binding properties

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12 December 2020

Peroxisomal multifunctional enzyme of beta-oxidation metabolizing D-3-hydroxyacyl-CoA esters in rat liver: molecular cloning, expression and characterization

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12 December 2020

High-level expression of Plasmodium vivax apical membrane antigen 1 (AMA-1) in Pichia pastoris: strong immunogenicity in Macaca mulatta immunized with P. vivax AMA-1 and adjuvant SBAS2.

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12 December 2020

Expression of Recombinant Human Soluble Type II Transforming Growth Factor-β Receptor inPichia pastorisandEscherichia coli:Two Powerful Systems to Express a Potent Inhibitor of Transforming Growth Factor-β

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12 December 2020

Chromophore incorporation, Pr to Pfr kinetics, and Pfr thermal reversion of recombinant N-terminal fragments of phytochrome A and B chromoproteins

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12 December 2020

Physiological effects and adjuvanticity of recombinant brushtail possum TNF-alpha

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12 December 2020

Isolation and expression of a cDNA clone encoding an Alternaria alternata Alt a 1 subunit

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12 December 2020

Purification and characterization of a soybean root nodule phosphatase expressed in Pichia pastoris

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12 December 2020

Expression of human cathepsin K in Pichia pastoris and preliminary crystallographic studies of an inhibitor complex

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12 December 2020

Expression of human amyloid precursor protein ectodomains in Pichia pastoris: analysis of culture conditions, purification, and characterization

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12 December 2020

Sequence polymorphism of the group 1 allergen of Bermuda grass pollen

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12 December 2020

Characterization of the reductase domain of rat neuronal nitric oxide synthase generated in the methylotrophic yeast Pichia pastoris. Calmodulin response is complete within the reductase domain itself

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12 December 2020

Overexpression, purification, and characterization of recombinant barley alpha-amylases 1 and 2 secreted by the methylotrophic yeast Pichia pastoris

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12 December 2020

Interaction between echovirus 7 and its receptor, decay-accelerating factor (CD55): evidence for a secondary cellular factor in A-particle formation.

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12 December 2020

High-level expression of uniformly 15N-labeled hen lysozyme in Pichia pastoris and identification of the site in hen lysozyme where phosphate ion binds using NMR measurements

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12 December 2020

Characterization of the extracellular ligand-binding domain of the type II activin receptor

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12 December 2020

Expression of the mammalian renal peptide transporter PEPT2 in the yeast Pichia pastoris and applications of the yeast system for functional analysis

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12 December 2020

Assembly of human prolyl 4-hydroxylase and type III collagen in the yeast Pichia pastoris: formation of a stable enzyme tetramer requires coexpression with collagen and assembly of a stable collagen requires coexpression with prolyl 4-hydroxylase

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12 December 2020

Processing, activity, and inhibition of recombinant cyprosin, an aspartic proteinase from cardoon (Cynara cardunculus).

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12 December 2020

Production and Characterization of Recombinant Active Mouse Gelatinase B from Eukaryotic Cells and in vivo Effects after Intravenous Administration

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12 December 2020

Labeling of peroxisomes with green fluorescent protein in living P. pastoris cells

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12 December 2020

High-level secretion of two antibody single chain Fv fragments by Pichia pastoris.

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12 December 2020

Expression of Reticulomyxa filosa tubulins in Pichia pastoris: regulation of tubulin pools

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12 December 2020

Expression, purification, and characterization of porcine leukocyte 12-lipoxygenase produced in the methylotrophic yeast, Pichia pastoris.

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12 December 2020

Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter

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12 December 2020

Inhibition of Aspergillus serine proteinase by Streptomyces subtilisin inhibitor and high-level expression of this inhibitor in Pichia pastoris

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12 December 2020

Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris

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12 December 2020

Emerging applications of the methylotrophic yeasts

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12 December 2020

Overexpression of active Syrian golden hamster prion protein PrPc as a glutathione S-transferase fusion in heterologous systems.

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12 December 2020

High-yield secretion of recombinant gelatins by Pichia pastoris

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12 December 2020

Production and purification of the heavy-chain fragment C of botulinum neurotoxin, serotype B, expressed in the methylotrophic yeast Pichia pastoris

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12 December 2020

Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies

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12 December 2020

High-level production of spinach glycolate oxidase in the methylotrophic yeast Pichia pastoris: engineering a biocatalyst

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12 December 2020

Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles

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12 December 2020

Cloning, mutagenesis, and structural analysis of human pancreatic alpha-amylase expressed in Pichia pastoris

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12 December 2020

High-level expression of bovine beta-lactoglobulin in Pichia pastoris and characterization of its physical properties

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12 December 2020

Biochemical and antigenic characterization of a new dipeptidyl-peptidase isolated from Aspergillus fumigatus

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12 December 2020

Large-scale production in Pichia pastoris of the recombinant vaccine Gavac against cattle tick

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12 December 2020

High-level secretion of a wheat lipid transfer protein in Pichia pastoris

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12 December 2020

Reconstitution of Highly Expressed Human Heart Muscle Carnitine Palmitoyltransferase I

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12 December 2020

Production and purification of recombinant hirudin expressed in the methylotrophic yeast Pichia pastoris

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12 December 2020

Expression and characterization of recombinant mast cell tryptase

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12 December 2020

Recombinant Bordetella pertussis pertactin (P69) from the yeast Pichia pastoris: high-level production and immunological properties

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12 December 2020

Cloning and expression of Candida guilliermondii xylose reductase gene (xyl1) in Pichia pastoris

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12 December 2020

Large-scale expression, purification and characterization of small fragments of thrombomodulin: the roles of the sixth domain and of methionine 388

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12 December 2020

Heterologous protein expression in the methylotrophic yeast Pichia pastoris

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12 December 2020

Altered antigenicity of 'a' determinant variants of hepatitis B virus

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12 December 2020

Engineering a soluble extracellular erythropoietin receptor (EPObp) in Pichia pastoris to eliminate microheterogeneity, and its complex with erythropoietin

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12 December 2020

Two forms of human double-stranded RNA-specific editase 1 (hRED1) generated by the insertion of an Alu cassette.

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12 December 2020

Purification, potency, and efficacy of the botulinum neurotoxin type A binding domain from Pichia pastoris as a recombinant vaccine candidate.

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12 December 2020

Purification of native and recombinant double-stranded RNA-specific adenosine deaminases.

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12 December 2020

NonO enhances the association of many DNA-binding proteins to their targets

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12 December 2020

Vl-linker-Vh orientation-dependent expression of single chain Fv-containing an engineered disulfide-stabilized bond in the framework regions

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12 December 2020

Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris

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12 December 2020

Use of site-specific recombination to regenerate selectable markers

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12 December 2020

The bovine IFN-omega 1 is biologically active and secreted at high levels in the yeast Pichia pastoris

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12 December 2020

Sequencing and functional expression of the malonyl-CoA-sensitive carnitine palmitoyltransferase from Drosophila melanogaster

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12 December 2020

Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris

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12 December 2020

Riboflavin 5'-hydroxymethyl oxidation. Molecular cloning, expression, and glycoprotein nature of the 5'-aldehyde-forming enzyme from Schizophyllum commune

1 reference

12 December 2020

Mapping the pro-region of carboxypeptidase B by protein engineering. Cloning, overexpression, and mutagenesis of the porcine proenzyme

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12 December 2020

Expression of a lipocalin in Pichia pastoris : secretion, purification and binding activity of a recombinant mouse major urinary protein

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12 December 2020

High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris

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12 December 2020

Expression and processing of vertebrate acetylcholinesterase in the yeast Pichia pastoris

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12 December 2020

Expression, Purification, and Neurotrophic Activity of Amyloid Precursor Protein-Secreted Forms Produced by Yeast

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12 December 2020

High-level secretion of biologically active aprotinin from the yeast Pichia pastoris

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12 December 2020

Functional expression of recombinant spiny dogfish shark (Squalus acanthias) cytochrome P450c17 (17 alpha-hydroxylase/C17,20-lyase) in yeast (Pichia pastoris).

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12 December 2020

Production and purification of human fibroblast collagenase (MMP-1) expressed in the methylotrophic yeast Pichia pastoris

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12 December 2020

Expression of Human Interleukin-17 inPichia pastoris:Purification and Characterization

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12 December 2020

Expression and pharmacological characterization of the human mu-opioid receptor in the methylotrophic yeast Pichia pastoris

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12 December 2020

Recent advances in the expression of foreign genes in Pichia pastoris

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12 December 2020

Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of lcc1 and expression in Pichia pastoris

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12 December 2020

Cloning of clustered Streptomyces viridosporus T7A lignocellulose catabolism genes encoding peroxidase and endoglucanase and their extracellular expression in Pichia pastoris

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12 December 2020

Expression in Pichia pastoris and purification of Aspergillus awamori glucoamylase catalytic domain

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12 December 2020

Expression of Human Monocyte Chemoattractant Protein-1 in the YeastPichia pastoris

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12 December 2020

Intracellular Expression and Purification of the c- kit Receptor Kinase Domain in Pichia pastoris

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12 December 2020

NMR studies of a viral protein that mimics the regulators of complement activation

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12 December 2020

Efficient expression and secretion of recombinant alpha amylase in Pichia pastoris using two different signal sequences

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12 December 2020

The Solution Structure of Type II Antifreeze Protein Reveals a New Member of the Lectin Family

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12 December 2020

Production of large quantities of isotopically labeled protein in Pichia pastoris by fermentation

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12 December 2020

Protection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutinin

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12 December 2020

Human IFNγ Receptor Cytoplasmic Domain: Expression and Interaction with HuIFNγ

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12 December 2020

Synthesis and cleavage- secretion of enzymatically active rabbit angiotensin-converting enzyme in Pichia pastoris

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12 December 2020

Production of Vibrio cholerae accessory cholera enterotoxin (Ace) in the yeast Pichia pastoris

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12 December 2020

Expression and secretion of a biologically active glycoprotein hormone, ovine follicle stimulating hormone, by Pichia pastoris

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12 December 2020

Expression of recombinant HLA-DR2 molecules. Replacement of the hydrophobic transmembrane region by a leucine zipper dimerization motif allows the assembly and secretion of soluble DR alpha beta heterodimers.

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12 December 2020

Recombinant caspase-3 expressed in Pichia pastoris is fully activated and kinetically indistinguishable from the native enzyme.

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12 December 2020

Costimulation of T cell proliferation by a chimeric B7-2 antibody fusion protein specifically targeted to cells expressing the erbB2 proto-oncogene

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12 December 2020

High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene

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12 December 2020

Three-finger toxin fold for the extracellular ligand-binding domain of the type II activin receptor serine kinase

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12 December 2020

Molecular cloning, chromosomal mapping and tissue-specific expression of a novel human alpha1,2-mannosidase gene involved in N-glycan maturation

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12 December 2020

Cloning and expression in yeast Pichia pastoris of a biologically active form of Cyn d 1, the major allergen of Bermuda grass pollen.

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12 December 2020

Rapid Selection Using G418 of High Copy Number Transformants of Pichia pastoris for High–level Foreign Gene Expression

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12 December 2020

A refined kinetic analysis of plasminogen activation by recombinant bovine tissue-type plasminogen activator indicates two interconvertible activator forms

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12 December 2020

Expression of a synthetic gene encoding the anticoagulant-antimetastatic protein ghilanten by the methylotropic yeast Pichia pastoris

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12 December 2020

Generation of rabbit monoclonal antibody fragments from a combinatorial phage display library and their production in the yeast Pichia pastoris

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12 December 2020

Solution structure of an EGF module pair from the Plasmodium falciparum merozoite surface protein 1

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12 December 2020

Human Placental Alkaline Phosphatase: Expression inPichia pastoris,Purification and Characterization of the Enzyme

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12 December 2020

Cloning, expression, purification, and immunocharacterization of placental protein-14

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12 December 2020

Analysis of Pichia pastoris Components in Recombinant Human Serum Albumin by Immunological Assays and by HPLC with Pulsed Amperometric Detection

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12 December 2020

Protection of Mice Against a Lethal Influenza Challenge by Immunization with Yeast‐Derived Recombinant Influenza Neuraminidase

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12 December 2020

High-level expression and purification of coffee bean alpha-galactosidase produced in the yeast Pichia pastoris

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12 December 2020

Cloning of a novel constitutively expressed pectate lyase gene pelB from Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of the gene product expressed in Pichia pastoris

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12 December 2020

Quality and authenticity of heterologous proteins synthesized in yeast

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12 December 2020

Structural Studies of α-N-Acetylgalactosaminidase: Effect of Glycosylation on the Level of Expression, Secretion Efficiency, and Enzyme Activity

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12 December 2020

Purification and characterization of human procolipase expressed in yeast cells

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12 December 2020

Yeast-secreted bovine herpesvirus type 1 glycoprotein D has authentic conformational structure and immunogenicity

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12 December 2020

Pichia pastoris as a host system for transformations

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12 December 2020

Recombinant proteinase 3 (Wegener's antigen) expressed inPichia pastorisis functionally active and is recognized by patient sera

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12 December 2020

Overexpression and characterization of Aspergillus awamori wild-type and mutant glucoamylase secreted by the methylotrophic yeast Pichia pastoris: comparison with wild-type recombinant glucoamylase produced using Saccharomyces cerevisiae and Aspergi

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12 December 2020

Expression, Purification, and Inhibitory Properties of Human Proteinase Inhibitor 8

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12 December 2020

Role of the occluding loop in cathepsin B activity

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12 December 2020

Independently melting modules and highly structured intermodular junctions within complement receptor type 1

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12 December 2020

Expression and purification of recombinant human granzyme B from Pichia pastoris

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12 December 2020

Identification and cloning of prs a 1, a 32-kDa endochitinase and major allergen of avocado, and its expression in the yeast Pichia pastoris

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12 December 2020

High-level expression of mouse Mdr3 P-glycoprotein in yeast Pichia pastoris and characterization of ATPase activity

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12 December 2020

Characterization of LeMir, a root-knot nematode-induced gene in tomato with an encoded product secreted from the root

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12 December 2020

29 Large-scale production of human CD38 in yeast by fermentation

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12 December 2020

Expression of a Human Mutant Monocyte Chemotactic Protein 3 in Pichia pastoris and Characterization as an MCP-3 Receptor Antagonist

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12 December 2020

Functional expression of a mammalian acetylcholinesterase in Pichia pastoris: comparison to acetylcholinesterase, expressed and reconstituted from Escherichia coli

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12 December 2020

Binding of urokinase-type plasminogen activator-plasminogen activator inhibitor-1 complex to the endocytosis receptors alpha2-macroglobulin receptor/low-density lipoprotein receptor-related protein and very-low-density lipoprotein receptor involves

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12 December 2020

Characterization of wild-type and mutant alpha2-antiplasmins: fibrinolysis enhancement by reactive site mutant

1 reference

12 December 2020

High-level secretion in Pichia pastoris and biochemical characterization of the recombinant kringle 2 domain of tissue-type plasminogen activator

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12 December 2020

Production and detailed characterization of biologically active olive pollen allergen Ole e 1 secreted by the yeast Pichia pastoris

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12 December 2020

Expression, purification, and characterization of recombinant alpha-N-acetylgalactosaminidase produced in the yeast Pichia pastoris

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12 December 2020

Cloning, Sequencing, and Expression of anEscherichia coliAcid Phosphatase/Phytase Gene (appA2) Isolated from Pig Colon

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12 December 2020

Role of glycosylation in the functional expression of an Aspergillus niger phytase (phyA) in Pichia pastoris.

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12 December 2020

High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts.

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12 December 2020

Crystal structure of a recombinant alphaEC domain from human fibrinogen-420

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12 December 2020

Secretory expression and characterization of insulin in Pichia pastoris

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12 December 2020

Subcellular localization of the Alzheimer's disease amyloid precursor protein and derived polypeptides expressed in a recombinant yeast system.

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12 December 2020

Grass group I allergens (beta-expansins) are novel, papain-related proteinases

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12 December 2020

High-Level Expression of RecombinantAplysiaADP-Ribosyl Cyclase inPichia pastorisby Fermentation

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12 December 2020

Expressions of recombinant venom allergen, antigen 5 of yellowjacket (Vespula vulgaris) and paper wasp (Polistes annularis), in bacteria or yeast.

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12 December 2020

Expression and Characterization of Human Tissue Kallikrein Variants

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12 December 2020

Production and characterization of recombinant human proteinase inhibitor 6 expressed in Pichia pastoris

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12 December 2020

Expression and functional characterization of the mammalian intestinal peptide transporter PepT1 in the methylotropic yeast Pichia pastoris

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12 December 2020

Expression, crystallization and preliminary X-ray analysis of the two amino-terminal Ig domains of the neural cell adhesion molecule (NCAM)

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12 December 2020

Cloning and expression of a queen pheromone-binding protein in the honeybee: an olfactory-specific, developmentally regulated protein.

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12 December 2020

Comparison of natural and recombinant isoforms of grass pollen allergens.

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12 December 2020

A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris

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12 December 2020

Heterologous expression of wild type and deglycosylated human sex steroid-binding protein (SBP or SHBG) in the yeast, Pichia pastoris. Characterization of the recombinant proteins

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12 December 2020

O-Mannosylation of Pichia pastoris cellular and recombinant proteins.

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12 December 2020

Physicochemical and Immunochemical Properties of Recombinant Human Serum Albumin fromPichia pastoris

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12 December 2020

Hyperexpression of biologically active human chorionic gonadotropin using the methylotropic yeast, Pichia pastoris

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12 December 2020

Production of synthetic spider dragline silk protein in Pichia pastoris.

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12 December 2020

Development of recombinant vaccines for botulinum neurotoxin

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12 December 2020

Functional characterization of mitochondrial carnitine palmitoyltransferases I and II expressed in the yeast Pichia pastoris

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12 December 2020

High level expression of heterologous proteins in methylotrophic yeast Pichia pastoris.

1 reference

12 December 2020

Expression and characterization of the hepatitis E virus ORF3 protein in the methylotrophic yeast, Pichia pastoris

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12 December 2020

Recombinant expression of alliin lyase from garlic (Allium sativum) in bacteria and yeasts

1 reference

12 December 2020

Molecular and crystal properties of Bos d 2, an allergenic protein of the lipocalin family.

1 reference

12 December 2020

Atypical processing of amyloid precursor fusion protein by proteolytic activity in Pichia pastoris

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12 December 2020

A transforming mutation enhances the activity of the c-Kit soluble tyrosine kinase domain

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12 December 2020

Characterization of the Nucleoside Triphosphate Phosphohydrolase and Helicase Activities of the Reovirus λ1 Protein

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12 December 2020

Glycosylation properties of the Pichia pastoris-expressed recombinant kringle 2 domain of tissue-type plasminogen activator

1 reference

12 December 2020

Antibody screening for secreted proteins expressed in Pichia pastoris

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12 December 2020

Secretion of a variant of human single-chain urokinase-type plasminogen activator without an N-glycosylation site in the methylotrophic yeast, Pichia pastoris and characterization of the secreted product

1 reference

12 December 2020

Efficient expression of the gene for spinach phosphoribulokinase in Pichia pastoris and utilization of the recombinant enzyme to explore the role of regulatory cysteinyl residues by site-directed mutagenesis

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12 December 2020

The Significance of Peroxisomes in the Metabolism of One-Carbon Compounds in Yeasts

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12 December 2020

High level expression of the B. microplus Bm86 antigen in the yeast Pichia pastoris forming highly immunogenic particles for cattle.

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12 December 2020

The first immunoglobulin-like neural cell adhesion molecule (NCAM) domain is involved in double-reciprocal interaction with the second immunoglobulin-like NCAM domain and in heparin binding

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12 December 2020

Identification in vitro of a post-translational regulatory site in the hinge 1 region of Arabidopsis nitrate reductase

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12 December 2020

High-level secretion of biologically active recombinant porcine follicle-stimulating hormone by the methylotrophic yeast Pichia pastoris

1 reference

12 December 2020

Zinc-binding of endostatin is essential for its antiangiogenic activity

1 reference

12 December 2020

Processing of the Alzheimer's disease amyloid precursor protein in Pichia pastoris: immunodetection of alpha-, beta-, and gamma-secretase products.

1 reference

12 December 2020

Structural investigations of a new familial dysalbuminemic hyperthyroxinemia genotype

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12 December 2020

An NMR-based identification of peptide fragments mimicking the interactions of the cathepsin B propeptide

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12 December 2020

The N-terminus of gp130 is critical for the formation of the high-affinity interleukin-6 receptor complex.

1 reference

12 December 2020

Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris

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12 December 2020

Recombinant human amyloid precursor-like protein 2 (APLP2) expressed in the yeast Pichia pastoris can stimulate neurite outgrowth

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12 December 2020

Isotopically labeled bovine beta-lactoglobulin for NMR studies expressed in Pichia pastoris

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12 December 2020

Crystallization of rat procathepsin B.

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12 December 2020

Variation in N-linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast Pichia pastoris

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12 December 2020

The Golgi sialoglycoprotein MG160, expressed in Pichia pastoris, does not require complex carbohydrates and sialic acid for secretion and basic fibroblast growth factor binding

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12 December 2020

Effect of particulation on the immunogenic and protective properties of the recombinant Bm86 antigen expressed in Pichia pastoris

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12 December 2020

Biosynthetic production of type II fish antifreeze protein: fermentation by Pichia pastoris

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12 December 2020

Engineering Pichia pastoris for biocatalysis: co-production of two active enzymes

1 reference

12 December 2020

Design, total synthesis, and functional overexpression of the Candida rugosa lip1 gene coding for a major industrial lipase

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12 December 2020

A recombinant human angiostatin protein inhibits experimental primary and metastatic cancer

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12 December 2020

Potency and selectivity of the cathepsin L propeptide as an inhibitor of cysteine proteases

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12 December 2020

High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris

1 reference

12 December 2020

Expression of enzymatically active, recombinant barley alpha-glucosidase in yeast and immunological detection of alpha-glucosidase from seed tissue

1 reference

12 December 2020

Effect of chromophore exchange on the resonance Raman spectra of recombinant phytochromes

1 reference

12 December 2020

Expression of Biologically Active β Subunit of Bovine Follicle-Stimulating Hormone in the Methylotrophic YeastPichia pastoris

1 reference

12 December 2020

Disruption of the KEX1 gene in Pichia pastoris allows expression of full-length murine and human endostatin

1 reference

12 December 2020

Cloning, expression, purification, and characterization of the human broad specificity lysosomal acid alpha-mannosidase

1 reference

12 December 2020

A chimeric protein comprised of bovine herpesvirus type 1 glycoprotein D and bovine interleukin-6 is secreted by yeast and possesses biological activities of both molecules.

1 reference

12 December 2020

Expression and secretion of rabbit plasma cholesteryl ester transfer protein by Pichia pastoris

1 reference

12 December 2020

Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris

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12 December 2020

Secretion, purification and activity of two recombinant pepper endo-beta-1,4-glucanases expressed in the yeast Pichia pastoris

1 reference

12 December 2020

Cloning and expression of acetylcholinesterase from Electrophorus. Splicing pattern of the 3' exons in vivo and in transfected mammalian cells

1 reference

12 December 2020

The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris

1 reference

12 December 2020

Expression of a fusion protein of scFv-biotin mimetic peptide for immunoassay

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12 December 2020

The pas8 mutant of Pichia pastoris exhibits the peroxisomal protein import deficiencies of Zellweger syndrome cells--the PAS8 protein binds to the COOH-terminal tripeptide peroxisomal targeting signal, and is a member of the TPR protein family

1 reference

12 December 2020

Mutations in Either Nucleotide-Binding Site of P-glycoprotein (Mdr3) Prevent Vanadate Trapping of Nucleotide at Both Sites

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12 December 2020

Recombinant HMG1 protein produced in Pichia pastoris: a nonviral gene delivery agent

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12 December 2020

Cloning of the patatin-like latex allergen Hev b 7, its expression in the yeast Pichia pastoris and its immunological characterization

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12 December 2020

Secretion of recombinant human insulin-like growth factor I (IGF-I).

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12 December 2020

Identification of a novel pelD gene expressed uniquely in planta by Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of its protein product as an endo-pectate lyase

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12 December 2020

Crystal structure of recombinant human tissue kallikrein at 2.0 A resolution

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12 December 2020

Lethal effect of recombinant human Fas ligand in mice pretreated with Propionibacterium acnes.

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12 December 2020

Functional phytohemagglutinin (PHA) and Galanthus nivalis agglutinin (GNA) expressed in Pichia pastoris correct N-terminal processing and secretion of heterologous proteins expressed using the PHA-E signal peptide

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12 December 2020

Hexose oxidase from the red alga Chondrus crispus. Purification, molecular cloning, and expression in Pichia pastoris.

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12 December 2020

Expression of human gelatinase B in Pichia pastoris

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12 December 2020

Cloning, sequencing, and recombinant expression of the porcine inhibitor of carbonic anhydrase: a novel member of the transferrin family

1 reference

12 December 2020

A glycosyl hydrolase activity of mammalian reovirus sigma1 protein can contribute to viral infection through a mucus layer

1 reference

12 December 2020

Heterologous overexpression of human NEFA and studies on the two EF-hand calcium-binding sites

1 reference

12 December 2020

The polymerization pocket "a" within the carboxyl-terminal region of the gamma chain of human fibrinogen is adjacent to but independent from the calcium-binding site

1 reference

12 December 2020

Expression of biologically active human vascular endothelial growth factor in yeast

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12 December 2020

Expression and analysis of heparin-binding regions of the amyloid precursor protein of Alzheimer's disease.

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12 December 2020

Human cathepsin V functional expression, tissue distribution, electrostatic surface potential, enzymatic characterization, and chromosomal localization

1 reference

12 December 2020

Expression, purification, and characterization of a two domain carcinoembryonic antigen minigene (N-A3) in pichia pastoris. The essential role of the N-domain.

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12 December 2020

Structure of oligosaccharides on Saccharomyces SUC2 invertase secreted by the methylotrophic yeast Pichia pastoris

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12 December 2020

Functional Expression of Soluble Forms of Human CD38 in Escherichia coli and Pichia pastoris

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12 December 2020

Characterization of the acidic oligosaccharides assembled on the Pichia pastoris-expressed recombinant kringle 2 domain of human tissue-type plasminogen activator

1 reference

12 December 2020

cDNA cloning of the 43-kDa latex allergen Hev b 7 with sequence similarity to patatins and its expression in the yeast Pichia pastoris

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12 December 2020

Production and purification of N-terminal half-transferrin in Pichia pastoris.

1 reference

12 December 2020

Glycosylation of asparagine-28 of recombinant staphylokinase with high-mannose-type oligosaccharides results in a protein with highly attenuated plasminogen activator activity

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12 December 2020

Overexpression of Human Procarboxypeptidase A2 inPichia pastorisand Detailed Characterization of Its Activation Pathway

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12 December 2020

Processing, disulfide pattern, and biological activity of a sugar beet defensin, AX2, expressed in Pichia pastoris

1 reference

12 December 2020

Intracellular production of a major cytomegalovirus antigenic protein in the methylotrophic yeast Pichia pastoris

1 reference

12 December 2020

Cloning, expression, purification, and characterization of the murine lysosomal acid alpha-mannosidase

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12 December 2020

Comparative biochemical and pharmacological characterization of the mouse 5HT5A 5-hydroxytryptamine receptor and the human β2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris

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12 December 2020

Complete determination of disulfide forms of purified recombinant human serum albumin, secreted by the yeast Pichia pastoris

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12 December 2020

Production of active polyomavirus large T antigen in yeast Pichia pastoris

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12 December 2020

Hemagglutination inhibition of Cromer blood group antibodies with soluble recombinant decay-accelerating factor

1 reference

12 December 2020

Autocatalytic processing of recombinant human procathepsin L. Contribution of both intermolecular and unimolecular events in the processing of procathepsin L in vitro

1 reference

12 December 2020

Human pancreatic triglyceride lipase expressed in yeast cells: purification and characterization

1 reference

12 December 2020

Secretory production of recombinant urokinase-type plasminogen activator-annexin V chimeras in Pichia pastoris.

1 reference

12 December 2020

Isolation of a gene encoding a glycosylated cytokinin oxidase from maize

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12 December 2020

Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris

1 reference

12 December 2020

Dipeptidyl-peptidase IV secreted by Aspergillus fumigatus, a fungus pathogenic to humans

1 reference

12 December 2020

Cloning, expression, and properties of a nonneuronal secreted acetylcholinesterase from the parasitic nematode Nippostrongylus brasiliensis

1 reference

12 December 2020

Mutations in the nucleotide-binding sites of P-glycoprotein that affect substrate specificity modulate substrate-induced adenosine triphosphatase activity

1 reference

12 December 2020

Analysis of wild-type and mutant plant nitrate reductase expressed in the methylotrophic yeast Pichia pastoris.

1 reference

12 December 2020

The dacA gene of Bacillus stearothermophilus coding for D-alanine carboxypeptidase: cloning, structure and expression in Escherichia coli and Pichia pastoris

1 reference

12 December 2020

Characterization of novel cathepsin K mutations in the pro and mature polypeptide regions causing pycnodysostosis.

1 reference

12 December 2020

Heterologous expression of an engineered truncated form of human Lewis fucosyltransferase (Fuc-TIII) by the methylotrophic yeast Pichia pastoris

1 reference

12 December 2020

Optimization of the production of a honeybee odorant-binding protein by Pichia pastoris

1 reference

12 December 2020

The conformation of purified Toxoplasma gondii SAG1 antigen, secreted from engineered Pichia pastoris, is adequate for serorecognition and cell proliferation

1 reference

12 December 2020

Expression of trimeric CD40 ligand in Pichia pastoris: use of a rapid method to detect high-level expressing transformants

1 reference

12 December 2020

Production of the rat complement regulator, Crry, as an active soluble protein in Pichia pastoris

1 reference

12 December 2020

Purification, crystallization and preliminary X-ray crystallographic analysis of recombinant murine Golgi mannosidase IA, a class I alpha-mannosidase involved in Asn-linked oligosaccharide maturation

1 reference

12 December 2020

High level expression, purification, and characterization of the Kunitz-type protease inhibitor domain of protease nexin-2/amyloid beta-protein precursor

1 reference

12 December 2020

Improved tumour targeting by disulphide stabilized diabodies expressed in Pichia pastoris

1 reference

12 December 2020

A new tool for studying the molecular architecture of the fungal cell wall: one-step purification of recombinant trichoderma beta-(1-6)-glucanase expressed in Pichia pastoris

1 reference

12 December 2020

Ktr1p is an alpha-1,2-mannosyltransferase of Saccharomyces cerevisiae. Comparison of the enzymic properties of soluble recombinant Ktr1p and Kre2p/Mnt1p produced in Pichia pastoris

1 reference

12 December 2020

Structural analysis of the CD5 antigen. Expression, disulphide bond analysis and physical characterisation of CD5 scavenger receptor superfamily domain 1

1 reference

12 December 2020

High-Level Production of RecombinantGeotrichum candidumLipases in YeastPichia pastoris

1 reference

12 December 2020

Production of human tissue factor using the Pichia pastoris expression system

1 reference

12 December 2020

Functional and receptor binding characterization of recombinant murine macrophage inflammatory protein 2: sequence analysis and mutagenesis identify receptor binding epitopes.

1 reference

12 December 2020

Cloning and expression of ragweed allergen Amb a 6.

1 reference

12 December 2020

Production of recombinant human bile salt stimulated lipase and its variant in Pichia pastoris

1 reference

12 December 2020

Secretion of recombinant pro- and mature fungal alpha-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: the Lys-Arg motif is required for maturation.

1 reference

12 December 2020

Macromolecular antimicrobial glycoprotein, achacin, expressed in a methylotrophic yeast Pichia pastoris

1 reference

12 December 2020

Adjuvant and immunostimulating properties of the recombinant Bm86 protein expressed in Pichia pastoris

1 reference

12 December 2020

Cloning, expression, purification, and characterization of the acid alpha-mannosidase from Trypanosoma cruzi

1 reference

12 December 2020

Identifiers

DOI

10.1385/MB:16:1:23

1 reference

Dimensions Publication ID

30 July 2017

1013013866

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