Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration (Q30527850)

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English	Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration	scientific article

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scholarly article

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration (English)

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

author name string

Mohab A Al-Hinai

1

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Alan G Fast

2

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Eleftherios T Papoutsakis

3

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

language of work or name

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publication date

14 September 2012

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Europe PubMed Central

PMC publication ID

4 October 2017

Applied and Environmental Microbiology

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Europe PubMed Central

PMC publication ID

4 October 2017

78

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

22

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

8112-8121

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker

1 reference

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Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications

1 reference

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20 June 2018

SpoIIE Is Necessary for Asymmetric Division, Sporulation, and Expression of σ F , σ E , and σ G but Does Not Control Solvent Production in Clostridium acetobutylicum ATCC 824

1 reference

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20 June 2018

Inactivation of σ F in Clostridium acetobutylicum ATCC 824 Blocks Sporulation Prior to Asymmetric Division and Abolishes σ E and σ G Protein Expression but Does Not Block Solvent Formation

1 reference

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Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum

1 reference

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20 June 2018

Inactivation of σ E and σ G in Clostridium acetobutylicum Illuminates Their Roles in Clostridial-Cell-Form Biogenesis, Granulose Synthesis, Solventogenesis, and Spore Morphogenesis

1 reference

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Development and application of a method for counterselectable in-frame deletion in Clostridium perfringens

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Construction and characterization of a lactose-inducible promoter system for controlled gene expression in Clostridium perfringens

1 reference

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1 reference

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mRNA interferases, sequence-specific endoribonucleases from the toxin-antitoxin systems

1 reference

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Engineering solventogenic clostridia

1 reference

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Antisense-RNA-mediated decreased synthesis of small, acid-soluble spore proteins leads to decreased resistance of clostridium perfringens spores to moist heat and UV radiation

1 reference

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Construction and analysis of chromosomal Clostridium difficile mutants

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Use of targetrons to disrupt essential and nonessential genes in Staphylococcus aureus reveals temperature sensitivity of Ll.LtrB group II intron splicing

1 reference

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Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection

1 reference

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mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis.

1 reference

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A conjugation-based system for genetic analysis of group II intron splicing in Lactococcus lactis

1 reference

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20 June 2018

Antisense RNA downregulation of coenzyme A transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic Clostridium acetobutylicum fermentations

1 reference

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Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker

1 reference

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Design of antisense RNA constructs for downregulation of the acetone formation pathway of Clostridium acetobutylicum

1 reference

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The genome sequence of Clostridium tetani, the causative agent of tetanus disease

1 reference

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Bacterial group II introns and their association with mobile genetic elements

1 reference

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Northern, morphological, and fermentation analysis of spo0A inactivation and overexpression in Clostridium acetobutylicum ATCC 824.

1 reference

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20 June 2018

Development and characterization of a gene expression reporter system for Clostridium acetobutylicum ATCC 824

1 reference

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20 June 2018

Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum

1 reference

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20 June 2018

Counterselectable markers: untapped tools for bacterial genetics and pathogenesis

1 reference

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Use of time-lapse microscopy to visualize rapid movement of the replication origin region of the chromosome during the cell cycle in Bacillus subtilis

1 reference

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Rolling-circle replication of bacterial plasmids

1 reference

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The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain

1 reference

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1 reference

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In vivo methylation in Escherichia coli by the Bacillus subtilis phage phi 3T I methyltransferase to protect plasmids from restriction upon transformation of Clostridium acetobutylicum ATCC 824

1 reference

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20 June 2018

Expression of plasmid-encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production

1 reference

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Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci

1 reference

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Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of alpha-toxin in Clostridium perfringens-mediated gas gangrene

1 reference

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1 reference

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1 reference

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Vector construction, transformation, and gene amplification in Clostridium acetobutylicum ATCC 824.

1 reference

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1 reference

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1 reference

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based on heuristic

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Effects of ptb knockout on butyric acid fermentation by Clostridium tyrobutyricum

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Determination of plasmid copy number and stability in Clostridium acetobutylicum ATCC 824

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Inactivation of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1128/AEM.02214-12

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

PMC publication ID

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

PubMed publication ID

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

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