Simple, sensitive and accurate method for the quantification of prothrombin mRNA by using competitive PCR (Q28364250)

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A M Stapleton

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K Miyazawa

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R L Ryall

Nucleotide sequence of the gene for human prothrombin

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language of work or name

English

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publication date

15 May 2001

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published in

Biochemical Journal

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volume

356

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issue

Pt 1

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page(s)

111-20

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cites work

1 reference

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Inhibition of calcium oxalate crystal growth and aggregation by prothrombin and its fragments in vitro: relationship between protein structure and inhibitory activity.

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Quantitative RT-PCR: pitfalls and potential

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Developments in quantitative PCR.

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Quantification of mRNA using competitive RT-PCR with standard-curve methodology.

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Quantitative RT-PCR: limits and accuracy

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Technical aspects of quantitative competitive PCR.

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Limitations imposed by heteroduplex formation on quantitative RT-PCR.

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An evaluation of competitor type and size for use in the determination of mRNA by competitive PCR.

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Quantitative PCR. A survey of the present technology

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The nucleotide sequence of the rat cytoplasmic β–actin gene

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Calcium oxalate urolithiasis in the rat: is it a model for human stone disease? A review of recent literature

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Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family

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Quantitation of mRNA by the polymerase chain reaction

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cDNA sequence of rat prothrombin

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Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction

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Inhibition of growth and aggregation of calcium oxalate crystals in vitro--a comparison of four human proteins.

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Animal models of kidney stone formation: an analysis

2 June 2018

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A novel method for real time quantitative RT-PCR.

2 June 2018

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Quantitation of matrix Gla protein mRNA by competitive polymerase chain reaction using glyceraldehyde-3-phosphate dehydrogenase as an internal control

2 June 2018

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Quantitative measurement of mRNAs by polymerase chain reaction

2 June 2018

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Prenatal and postnatal expression of mRNA coding for rat prothrombin.

2 June 2018

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Differential regulation of transforming growth factor beta and interleukin 2 genes in human T cells: demonstration by usage of novel competitor DNA constructs in the quantitative polymerase chain reaction

2 June 2018

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PCR-based quantitation of low levels of HIV-1 DNA by using an external standard

2 June 2018

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Gene expression of prothrombin in the human kidney and its potential relevance to kidney stone disease.

2 June 2018

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Matrix Gla protein gene expression is elevated during postnatal development.

27 November 2018

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Quantitative PCR: theoretical considerations with practical implications.

27 November 2018

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Prothrombin gene expression in rat kidneys provides an opportunity to examine its role in urinary stone pathogenesis

27 November 2018

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Immunohistochemical distribution and quantification of crystal matrix protein

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Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity

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