Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme (Q24559878)

19 October 2023

S C Blacklow

series ordinal

2

author given names

S C

author last names

Blacklow

1 reference

19 October 2023

J R Knowles

series ordinal

3

author given names

J R

author last names

Knowles

1 reference

Proceedings of the National Academy of Sciences of the United States of America

19 October 2023

language of work or name

English

0 references

publication date

January 1990

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published in

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volume

87

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issue

2

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page(s)

696-700

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cites work

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Structure of chicken muscle triose phosphate isomerase determined crystallographically at 2.5Å resolution: using amino acid sequence data

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Free-energy profile of the reaction catalyzed by triosephosphate isomerase

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27 September 2017

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Tinkering with enzymes: what are we learning?

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A new method for random mutagenesis of complete genes: enzymatic generation of mutant libraries in vitro.

27 September 2017

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Improved peptide function from random mutagenesis over short 'windows'.

27 September 2017

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Characterization of the gene for the microbody (glycosomal) triosephosphate isomerase of Trypanosoma brucei

27 September 2017

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The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.

27 September 2017

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Strategies and applications of in vitro mutagenesis

27 September 2017

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Improved oligonucleotide site-directed mutagenesis using M13 vectors.

27 September 2017

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DNA binding specificity of the Arc and Mnt repressors is determined by a short region of N-terminal residues

27 September 2017

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The amino acid sequence of rabbit muscle triose phosphate isomerase

27 September 2017

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Site-directed mutagenesis in DNA: generation of point mutations in cloned beta globin complementary dna at the positions corresponding to amino acids 121 to 123

27 September 2017

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Molecular basis of base substitution hotspots in Escherichia coli

27 September 2017

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Nucleotide sequence of the triose phosphate isomerase gene of Escherichia coli

27 September 2017

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Triose phosphate isomerase from the coelacanth. An approach to the rapid determination of an amino acid sequence with small amounts of material

30 May 2018

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Active-site labelling of triose phosphate isomerase. The reaction of bromohydroxyacetone phosphate with a unique glutamic acid residue and the migration of the label to tyrosine

30 May 2018

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Specificity and kinetics of triose phosphate isomerase from chicken muscle

30 May 2018

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Transcription of the triose-phosphate-isomerase gene of Schizosaccharomyces pombe initiates from a start point different from that in Saccharomyces cerevisiae

30 May 2018

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The triosephosphate isomerase gene from maize: introns antedate the plant-animal divergence

30 May 2018

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Nucleotide sequence of the triosephosphate isomerase gene from Aspergillus nidulans: implications for a differential loss of introns

30 May 2018

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Role of arginine-292 in the substrate specificity of aspartate aminotransferase as examined by site-directed mutagenesis.

30 May 2018

1 reference

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A general method for saturation mutagenesis of cloned DNA fragments

30 May 2018

1 reference

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Phage lambda repressor revertants. Amino acid substitutions that restore activity to mutant proteins

30 May 2018

1 reference

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Bacterial mutator genes and the control of spontaneous mutation

30 May 2018

1 reference

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Triosephosphate isomerase catalysis is diffusion controlled. Appendix: Analysis of triose phosphate equilibria in aqueous solution by 31P NMR.

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53332

Reaction energetics of a mutant triosephosphate isomerase in which the active-site glutamate has been changed to aspartate

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53332

Changing the binding specificity of a repressor by redesigning an alpha-helix

28 November 2018

1 reference

12 December 2020

Irreversible inactivation of triose phosphate isomerase by 1-hydroxy-3-iodo-2-propanone phosphate

1 reference

12 December 2020

1 reference

12 December 2020

A specific, highly active malate dehydrogenase by redesign of a lactate dehydrogenase framework

1 reference

12 December 2020

A simple and efficient procedure for generating random point mutations and for codon replacements using mixed oligodeoxynucleotides

1 reference

12 December 2020

Redesigning trypsin: alteration of substrate specificity

1 reference

12 December 2020

Crystallography and site-directed mutagenesis of yeast triosephosphate isomerase: what can we learn about catalysis from a "simple" enzyme?

1 reference

12 December 2020

Primary structure of triosephosphate isomerase from Bacillus stearothermophilus

1 reference

12 December 2020

The mechanism of the triosephosphate isomerase reaction.

1 reference

12 December 2020

The 14th barrel rolls out

1 reference

12 December 2020

release_nmo7gsypsnhdjdmlhjmmlq34e4

Identifiers

DOI

10.1073/PNAS.87.2.696

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ADS bibcode

1990PNAS...87..696H

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Fatcat ID

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PMC publication ID

53332

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