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    ENTEROBACTERIACEAE

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    ENTEROBACTERIACEAE

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    6 views19 pages

    ENTEROBACTERIACEAE

    Uploaded by

    joshuafadama62

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 19

    ENTEROBACTERIACEAE

    Objectives
     List the general characteristics of organisms that
    belong to the family Enterobacteriaceae.
     Describe the antigenic structures of the family
    Enterobacteriaceae, and explain how these
    antigens are used for identification.
     Compare the virulence factors of the Escherichia
    coli strains pathogenic for the gastrointestinal
    tract and the E. coli strains involved in extra
    intestinal diseases.
     Compare the pathogenesis of the three species of
    Yersinia most often recovered from humans.
     Describe the pathogenesis of some of the
    clinically relevant members of the family
    Enterobacteriaceae.
    Enterobacteriaceae
     Enterobacteriaceae are ubiquitous
    bacteria.
     Present as part of the normal intestinal
    flora of humans and animals.
     Non sporing, catalase positive, nonacid
    fast, and Gram-negative bacilli.
     Non fastidious
     They are Gram negative bacilli
     They reduce nitrate to nitrite as part of
    their energy generating processes
    Enterobacteriaceae
     They are often referred to as enterics, and consists of numerous diverse

    organisms

     Enterobacteriaceae have several key laboratory features in common

     They are facultative anaerobes

     They all ferment glucose

     They do not produce cytochrome oxidase, except Plesiomonas

     Motile by peritrichous flagella except Klebsiella, Shigella and Yersinia

    5
    Enterobacteriaceae
     Colony morphology on non selective
    media like BA,CA is of little value in
    identification-large, moist, gray colonies,
    except:
     Klebsiella and sometimes Enterobacter
    produce large and mucoid colonies
     Many isolates of E. coli are beta haemolytic
     Proteus exhibit swarming motility
    Enterobacteriaceae
     Differential and
    selective media; EMB
    Hektoen enteric (HE),
    xylose-lysine
    desoxycholate (XLD)
    and MAC agars- give
    presumptive
    identification.
     LF-Escherichia,
    Klebsiella and some
    Enterobacter species
    and more slowly by
    Citrobacter
    Enterobacteriaceae
    Enterobacteriaceae
    Enterobacteriaceae
     NLF-lack of color change-Proteus,
    Salmonella, Shigella
     Hydrogen sulphide producers may be
    readily distinguished when placed on HE or
    XLD
    agar. HE and XLD agars contain sodium
    thiosulphate and ferric ammonium citrate,
    which produce blackening
     Definitive identification depends
    biochemical and serological reactions
    CLASSIFICATION OF
    ENTEROBACTERIACEAE
     Based on
     Biochemical reactions
     Serological reactions
     DNA homology
    BIOCHEMICAL REACTIONS
    • IMViC test, Urease etc
    IMViC characteristics for common
    Enterobacteriaceae
    SEROLOGICAL CLASSIFICATION
     O antigens, or somatic antigen-heat-stable
    located on the cell wall (cell wall antigen).
     H antigens, or flagellar antigen-heat-labile
    found on the surface of flagella
     K antigens, or capsular antigens-heat-labile.
    They surround the cell wall
     Vi antigen of Salmonella enterica subsp.
    enterica serotype Typhi
    Ewing’s classification of the family
    Enterobacteriaceae
    Other classifications
    Primary pathogens Opportunistic pathogens
     Salmonella  E coli
     Shigella  Klebsiella
     Yersinia  Proteus
     E coli (diarrhoeagenic)  Serratia etc
    Schematic diagram showing variety of diseases caused by
    the members of the family Enterobacteriaceae in humans.
    Human infections caused by common
    members of the family Enterobacteriaceae
     Rajesh Bhatia and Rattan Lal
    Ichhpujani (2008). Essentials of
    Medical Microbiology. 4th Edition.
    Jaypee Brothers

     Ryan K.J.,Ray C.G., N. Ahmed and w.l.


    Drew (2014) Sherris medical
    microbiology:6th Edition McGraw
    Hill. Medical New York.

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