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IVF(In vitro Fertilization)
Miss Afshan Arshad
Introduction • Couples worldwide. It is regarded as psychologically stressful by most individuals and can lead to depression, social isolation and a lower quality of life. Historically, little medical help has been available to infertile individuals, who were therefore forced to risk their health and even lives, by taking part in more or less obscure infertility- treatment practices. Female infertility is often he sperm . whereas male infertility is linked to impaired sperm quantity and quality In Vitro Fertilization (IVF)
• In vitro fertilization (IVF) is the process by which a female egg is
fertilized outside the body in a laboratory with male sperm, typically in a cell culture dish. The fertilized egg can then be implanted in the uterus and will attempt to attach and develop inside the female. In some cases, the eggs, sperm or embryos may be frozen, preserved and stored for use at a later date IVF: In vitro fertilization
• In vitro: in glass
• Fertilization: Ova + Sperm
Fertilized eggs (now referred to as embryo or blastocyst) is implanted into uterus 3 Eggs retrieved Sperm sample provided Egg from ovaries production
Egg and sperm
combined and incubated to allow fertilization Hormonal Treatments
• Drugs currently in use include:
• clomiphene citrate (Clomidâ, Seropheneâ) • human menopausal gonadotropin (hCG) • gonodotropin releasing hormone (GnRH) analog called leuprolide (Lupronâ)
• Most of these drugs may be used alone or in a combination with others.
Egg Harvest
• Ultra Sound Guided Aspiration
• Laproscopy Fig 1 Oocytes with granulosa cells
"Naked" Oocyte 8-cell embryo for transfer Blastocyst for transfer Alternates of IVF
• Gamete intrafallopian transfer (GIFT): GIFT is similar to IVF. It is used
when a woman has at least one normal fallopian tube. Eggs are placed in this tube along with a man’s sperm to fertilize there. • Zygote intrafallopian transfer (ZIFT): ZIFT is tubal embryo transfer in which a woman’s eggs are taken from her ovaries, fertilized in the laboratory, and put back in the fallopian tubes rather than the uterus. • Assisted fertilization techniques when not enough sperm are available or sperm quality is not sufficient to fertilize include the following: • Intracytoplasmic sperm injection • Embryo cryopreservation (frozen fertilized egg and sperm) ICSI Stands for intracytoplasmic sperm injection. This process is used to inject a single sperm into each egg before the fertilized eggs are put back into the woman's body. The procedure may be used if the male has a low sperm count. Cryopreservation of Ova, Sperm and Embryo