Scribd Logo
Upload

Welcome to Scribd!

  • 51 views

    Quality Control in Microbiology and Serology

    Uploaded by

    W.F Kareem
    This document discusses quality control in microbiology and serology laboratories. It describes three phases of quality control: pre-analytical, analytical, and post-analytical. The pre-analytical phase involves specimen collection, transport, and criteria for rejection. The analytical phase covers processing, culture media quality control, equipment quality control, and aseptic techniques. The post-analytical phase includes report release, record keeping, and following standard operating procedures. Maintaining quality control is important for accurately detecting deficiencies and improving laboratory results.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd

    Quality Control in Microbiology and Serology

    Uploaded by

    W.F Kareem
    51 views10 pages
    This document discusses quality control in microbiology and serology laboratories. It describes three phases of quality control: pre-analytical, analytical, and post-analytical. The pre-analytical phase involves specimen collection, transport, and criteria for rejection. The analytical phase covers processing, culture media quality control, equipment quality control, and aseptic techniques. The post-analytical phase includes report release, record keeping, and following standard operating procedures. Maintaining quality control is important for accurately detecting deficiencies and improving laboratory results.

    Original Title

    Quality Control in microbiology and serology

    Copyright

    © © All Rights Reserved

    Available Formats

    PPTX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document discusses quality control in microbiology and serology laboratories. It describes three phases of quality control: pre-analytical, analytical, and post-analytical. The pre-analytical phase involves specimen collection, transport, and criteria for rejection. The analytical phase covers processing, culture media quality control, equipment quality control, and aseptic techniques. The post-analytical phase includes report release, record keeping, and following standard operating procedures. Maintaining quality control is important for accurately detecting deficiencies and improving laboratory results.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    51 views10 pages

    Quality Control in Microbiology and Serology

    Uploaded by

    W.F Kareem
    This document discusses quality control in microbiology and serology laboratories. It describes three phases of quality control: pre-analytical, analytical, and post-analytical. The pre-analytical phase involves specimen collection, transport, and criteria for rejection. The analytical phase covers processing, culture media quality control, equipment quality control, and aseptic techniques. The post-analytical phase includes report release, record keeping, and following standard operating procedures. Maintaining quality control is important for accurately detecting deficiencies and improving laboratory results.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 10

    Quality Control

    In Microbiology & Serology

    Presentation By: Submitted To:


    Hajra Mustafa
    Fatima Javed
    Mam Shazia
    Warda Faiz
    Mehboob Saghir
    MLT 3rd Year
    Quality Control

    It is designed to
     Detect
     Reduce
     Correct
    The deficiencies in laboratory. This is done prior to the release of reports to
    improve the quality of results.

    Types Of Quality Control:


    1. Internal QC
    2. External QC
    Phases of Quality Control

    There are three main phases of Quality Control in laboratory;


    1. Pre-analytical Phase
    2. Analytical Phase
    3. Post-analytical Phase
    Pre-Analytical Phase

    In pre-analytic phase of quality control, following steps should be followed to


    maintain check & balance in microbiology and serology lab.

    1. Specimen Collection
    2. Specimen Transport
    3. Criteria for Rejection of Specimen

    Specimen Collection:
    Samples should be collected appropriatly. Check for the patient’s name and
    serial numbers.
    Specimen Transport:
    Specimen should be handed over carefully to respective laboratory. Technologist
    should make sure that samples are labelled and sealed.

    Criteria for Rejection of Specimen:


    Following is the list of criteria to reject inappropriate specimens:
     Leaking Container
     Dried Samples
     Hemolysed Blood
     Wrong Tube
     Unlabelled Samples/Mismatched Samples
    Analytical Phase
    Processing Of Specimen:

    1. Select the primary culture media for specimen.


    2. Select the temperature and conditions for incubation.
    3. Transfer specimen on a culture plate in a biosafety cabinet.

    QC Of Culture Media:

    4. Water used for the media should be free of Copper ions.


    5. Media prepared should be free from excessive bubbles or pits.
    6. Should be free of cracks, freezing and crystallization.
    7. Should be checked at four points being at right angles to each other.
    QC Of Culture Plate:

    1. The wire is sterilized by holding it vertically in the flame of burner so that the
    length of wire becomes red hot.
    2. It is allowed to cool down before it touches any matetial suspected to be
    having bacteria to avoid heat killing the oraganisms.
    3. Essential to learn the skills of inoculating specimen.
    4. Instrument for seeding media selected according to the nature of medium and
    inoculum.
    5. Platinum or nichrome wires of different guages are used.
    QC of Stains and Reagents:

    1. All stains and reagents must be clearly labelled, dated and stored correctly.
    2. Should not be used beyond expiry date.
    3. Smears should not be very thick.

    Antimicrobial susceptibilty Testing:

    4. Use appropriate control cultures.


    5. Use cooled stains from time to time to maintain internal QC.
    6. Space the antibiotic discs properly to avoid overlapping of inhibition zone.
    QC Of Equipments:

    1. Check waterbath level daily. Clean monthly and check temperature.


    2. Discard chipped glassware. Ensure these are free of detergents.Do not store
    sterile glassware for more than 3 weeks.
    3. Centrifuge: Check revolution by tachometer. Wipe inner walls with antiseptic
    solution weekly. Check brushes and bearings every 6 months.
    4. Inspissator: Check temperatues daily. Clean after each batch of culture
    media.
    Aspectic Techniques:
    5. Wash hands with soap and water before and after handling infectious
    specimens.
    6. Keep all specimens in racks in order to prevent accidental spillage.
    7. Mop up the workbench clean with disinfectant at the start and end of work.
    Post-Analytical Phase

    1. Release of reports only to the authorized person.


    2. Timely release of provisional and final reports.
    3. A copy report should also be kept in lab.
    4. All patients records should be maintained for at least 2 years and ideally for
    10 years.
    Standard Operating Procedure (SOP):
    5. Essential component of QC.
    6. Provide staff with written instructions on how to perform test consistently
    according to an accteptable standard.
    7. Avoid short-cuts while performing tests.

    You might also like