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    Sterilization of Fermenter-1

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    Shalini M
    This document discusses the sterilization of fermenters. It defines sterilization as the complete removal of microorganisms from a surface. Fermenters are devices used for fermentation processes utilizing microorganisms. They can be sterilized with steam either when empty or with the production medium inside. Steam sterilization involves filling the fermenter jacket with pressurized steam for at least 20 minutes to achieve sterilization. Connections to ancillary equipment like agitators and sampling ports must also be properly sterilized to prevent contamination.

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    Sterilization of Fermenter-1

    Uploaded by

    Shalini M
    2K views11 pages
    This document discusses the sterilization of fermenters. It defines sterilization as the complete removal of microorganisms from a surface. Fermenters are devices used for fermentation processes utilizing microorganisms. They can be sterilized with steam either when empty or with the production medium inside. Steam sterilization involves filling the fermenter jacket with pressurized steam for at least 20 minutes to achieve sterilization. Connections to ancillary equipment like agitators and sampling ports must also be properly sterilized to prevent contamination.

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    sterilization of fermenter-1

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    This document discusses the sterilization of fermenters. It defines sterilization as the complete removal of microorganisms from a surface. Fermenters are devices used for fermentation processes utilizing microorganisms. They can be sterilized with steam either when empty or with the production medium inside. Steam sterilization involves filling the fermenter jacket with pressurized steam for at least 20 minutes to achieve sterilization. Connections to ancillary equipment like agitators and sampling ports must also be properly sterilized to prevent contamination.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    2K views11 pages

    Sterilization of Fermenter-1

    Uploaded by

    Shalini M
    This document discusses the sterilization of fermenters. It defines sterilization as the complete removal of microorganisms from a surface. Fermenters are devices used for fermentation processes utilizing microorganisms. They can be sterilized with steam either when empty or with the production medium inside. Steam sterilization involves filling the fermenter jacket with pressurized steam for at least 20 minutes to achieve sterilization. Connections to ancillary equipment like agitators and sampling ports must also be properly sterilized to prevent contamination.

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    © All Rights Reserved
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    Download as pptx, pdf, or txt
    of 11

    THE OXFORD COLLEGE OF

    SCIENCE
    STERILIZATION OF FERMENTERS

    UNDER THE GUIDANCE OF KARTHIK S N


    PRAJEESH MSc IV SEM
    DEPT OF MICROBIOLOGY
    CONTENTS

    • STERILIZATION
    • FERMENTER
    • OBEJECTIVES OF FERMENTER
    • STERILIZATION OF FERMENTER
    • REFERENCES
    STERILIZATION OF FERMENTER
    STERILIZATION
    • Complete removal of micro-organisms both vegetative and spore forms
    from the surface or an objects.
    • Sterilization is done to avoid growth of micro-organisms.
    FERMENTER
    • Device that is used to carry out fermentation process utilizing micro-
    organisms.
    • Also known as bioreactor.
    OBJECTIVES OF FERMENTER
    STERILIZATION OF FERMENTER

    • Steam is a physical agent universally employed for sterilization of


    fermenter and production medium.
    • It exists a linear relationship between time and temperature.
    • Fermenter are sterilized either in charged condition or they may be
    sterilized empty.
    1. STERILIZATION OF FERMENTER AND MEDIUM AS A UNIT

    • Steam sterilization of fermenter and production medium together is a


    simple operation.
    • A jacket or coil is filled to the fermenter and supplied with pressure
    steam.
    • Water is required to provide temperature for a period of at least 20
    minutes and after subsequent cooling back to fermentation
    temperature.
    • Head space above the medium is filled with steam.
    • The presence of vacuum in a sterile system is always a hazard to
    sterility it is due to danger of drawing non-sterile air or liquid into the
    sterile system through seals on agitator.
    Ancillary equipment includes many extra-connections they are;
    i. Agitator shaft passing through the lid of the vessels: There should be
    adequate seal for maintaining pressure to prevent the entry of microbes.
    ii. Sparger: A pipe for expelling exhausted air from fermenter.
    iii. Connection between defoamer tank and fermenter.
    iv. Connection for introducing the inoculum.
    v. Connection for drawing samples during fermentation.
    vi. Connection for unloading the fermenter after the fermentation.
    2. STERILIZATION OF FERMENTER AND MEDIUM SEPARATELY
    • Sterilization of empty fermers is same as described above the
    fermenter and production medium together.
    • There are several other methods for sterilizing the medium separately
    from the fermenter.
    • Certain points which are important while sterilizing and maintaining
    sterile conditions of fermentation equipment they are:
    i. No connections should be made permanently between the sterile
    and non-sterile parts the system.
    ii. Welded construction should be used.
    iii. Using of high quality value and rubber.
    iv. Joints and service lines should be carefully selected
    v. All sterilized parts should be kept under positive pressure.
    vi. Each part should be capable of independently sterilization without
    interfering with the operations of rest of the plant.
    REFERENCES

    Industrial microbiology- A.H Patel


    https://byjus.com
    https://www.slideshare.net/RjatSngh/fermentor-75732789

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