Biometrik2 Week3a

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Experimental Design

An Experimental Design is a plan for the assignment

of the treatments to the plots in the experiment Designs differ primarily in the way the plots are grouped before the treatments are applied
How much restriction is imposed on the random assignment of treatments to the plots
A C B B D A D B D A C C A C B B D A D B D C A C

Why do I need a design?


To provide an estimate of experimental error
To increase precision (blocking) To provide information needed to perform tests of

significance and construct interval estimates To facilitate the application of treatments particularly cultural operations

Factors to be Considered

Physical and topographic features Soil variability Number and nature of treatments The crop Duration of the experiment Machinery to be used Size of the difference to be detected Significance level to be used Experimental resources Cost (money, time, personnel)

Cardinal Rule:
Choose the simplest experimental design that will give the required precision within the limits of the available resources

Completely Randomized Design (CRD)


Simplest and least restrictive Every plot is equally likely to be assigned to any

treatment

C
B

D
A

B
D

C
C

Advantages of a CRD
Flexibility Any number of treatments and any number of replications Dont have to have the same number of replications per treatment (but more efficient if you do)
Simple statistical analysis Even if you have unequal replication

Missing plots do not complicate the analysis


Maximum error degrees of freedom

Disadvantage of CRD
Low precision if the plots are not uniform

A
C B

B
D A

D
B D

A
C C

Uses for the CRD


If the experimental site is relatively uniform If a large fraction of the plots may not

respond or may be lost If the number of plots is limited

Design Construction
No restriction on the assignment of treatments to the plots
Each treatment is equally likely to be assigned to any plot Should use some sort of mechanical procedure to prevent

personal bias Assignment of random numbers may be by:


lot (draw a number ) computer assignment using a random number table

Random Assignment by Lot


We have an experiment to test three varieties: the

top line from Oregon, Washington, and Idaho to find which grows best in our area ----- t=3, r=4

A1
A

2
A

3 7
A

4 8

1 12 6 5

9 10 11 12

Random Assignment by Table


Select a starting point somewhere

in a table of random numbers Moving up or down, left or right, write down the first n (12) three digit numbers you come to Rank the numbers from smallest to largest Assign the first treatment to the first r (4) numbers in sequence, etc.

49416 97191 39100 53988 33423 81899 61547 17520 34502

44448 53798 17217 71087 67453 07449 18707 95401 75508

04269 12693 74073 84148 43269 37985 85498 55811 06059

Tabel Bilangan Teracak


14620 09724 95430 85125 12951 48477 81953 42783 17629 70473 19082 15232 73645 84146 09182 87729 73649 65584 56823 83641

56919
97310 07585

17803
78209 27040

95781
51263 26939

85069
52396 64531

61594
82681 70570

94252
72020 48392

77489
18895 06359

62434
84948 47040

20965
53072 05695

20247
74573 79799

25950 82937 60819 59041 74208

85189 16405 27364 37475 69516

69374 81497 59081 03615 79530

37904 20863 72635 84093 47649

06759 94072 49180 49731 53046

37950 09394 34800 36435 28187

77387 59842 28055 75946 31824

35495 39573 91570 85712 52265

78192 51630 99154 06293 80494

84518 78548 39603 85621 66428

39412 48480 95318

03642 50075 28749

87497 11804 49512

29735 24956 35408

14308 72182 21814

72094
63158

16385
49753

90185
84279

72635
56496

86259
30618

BILANGAN ACAK
937 149 908 361 953 749 180

URUTAN
1 2 3 4 5 6 7

PERINGKAT
21 2 19 10 23 17 4

PERLAKUAN
A1 A2 A3 A4 B1 B2 B3

951
018 427 918 772 243 494 704 549 957 157 571 226 195 278 506 239

8
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

22
1 11 20 18 8 12 16 14 24 3 15 6 5 9 13 7

B4
C1 C2 C3 C4 D1 D2 D3 D4 E1 E2 E3 E4 F1 F2 F3 F4

1
C 5 F 9 F 13

2
A 6 E 10 A 14

3
F 7 F 11 C 15

4
B 8 D 12 D 16

A1
17 B 21 A 22 18

D1
19 C 23 B

E
20 A 24 B

The CRD Analysis


We can: Estimate the treatment means Estimate the standard error of a treatment mean Test the significance of differences among the treatment means

RANCANGAN ACAK LENGKAP FULLY RANDOMIZED DESIGN UNGROUPED EXPERIMENT


1 7
13 19

2 8
14 20

3 9
15 21

4 10
16 22

5 11
17 23

6 12
18 24

Hipotesis : Ho = t1 = t2 = t3 = t4 = t5=t6 H1 = t1 t2 t3 t4 t5t6 Fh F.05 = Berbeda nyata Fh F.05 = Berbeda Tidak nyata

Jumlah Ulangan = (R-1) (T-1) 15

PERCOBAAN RANCANGAN ACAK LENGKAP (PRAKTIKUM 1)


PERLAKUAN A B C 43,1 42,3 41,1 ULANGAN 42,9 42,4 41,2 43,4 41,8 41,4 43,0 42,2 40,9 TOTAL RATA-RATA

D
E F TOTAL

39,9
37,2 37,0

40
38,2 36,8

39,7
38,6 37,4

39,2
37,7 36,5

Contoh penelitian RAL


Pengujian beberapa insektisida parasit dan telur serta hama

penggerek (Triporyza incertulas Walker) telah dilakukan di Lab. Hama dan Penyakit tanaman Faperta Unpad Jatinangor. Ada enam insektisida yang dipakai: Dursban 20 EC, Reldan 24 EC, Lebaycid 550 EC, Trebon 10 EC, Curacron dan Diazinon 60 EC. Konsentrasi yang digunakan adalah 2cc/l. Respon yang diamati adalah persentase parasit telur penggerek yang menetas. Jelaskan kesimpulan penelitian tersebut !!!

Perlakuan

1 56,0
54,4 41,2 49,5 59,1 35,7
295,9

2 48,2
44,3 38,5 52,1 57,6 38,4
279,1

Total 208,6
198,6 159,8 197,8 229,5 155,6
954,1

Rata 51,65
49,05 39,95 49,45 57,38 38,90

A
B C D E F Total

51,8 52,6
49,5 50,4 35,4 44,7 50,4 45,8 60,2 52,6 40,2 41,3
287,5 287,4

CARA PERHITUNGAN:
GT2 FK = ---------- = (954,1)2 : 24 =37929,45 Txr

JK Total = ( Yij2 FK = + .. + .. + ... FK = . JK. Perl. = A.2+ .+ F2 FK = 4 JK Total = 562 +.+ 41,372 FK = JK Galat = JK total JK Perlakuan = KT = JK/db galat

Tabel Sidik Ragam (ANOVA)


Sumber Ragam DB JK KT Fh F.05

Perlakuan

18195

3639

271,56

2,09

Galat

19

255

13,4

Total

24

18450

768,7

RANCANGAN ACAK LENGKAP


Untuk memperoleh manisan pepaya yang baik, sebelum
direndam dalam larutan sukrosa harus dilakukan blansing terlebih dahulu dengan tujuan untuk menginaktifkan

enzim-enzim. Masalahnya adalah berapa lama harus


dilakukan blansing tersebut. Seorang peneliti telah melakukan percobaan dengan 6 waktu blansing, yaitu: 3,

6, 9, 12, 15 dan 18 menit.

Rancangan Acak Lengkap


Perlakuan 1 2 Ulangan 3 4 Total Rata - rata

A B C D E F Total

43,1 42,3 41,1 39,9 37,2 37,0 240,6

42,9 42,4 41,2 40,0 38,2 36,8 241,5

43,4 41,8 41,4 39,7 38,6 37,4 242,3

43,0 42,2 40,9 39,2 37,7 36,5 239,5

172,4 168,7 164,6 158,8 151,7 147,7 963,9

43,10 42,17 41,15 39,70 37,92 36,92

Tabel Analisis Varians (ANOVA) RAL


Sumber Variasi Perlakuan Galat Total 5 18 23 db Jumlah Kuadrat 117,42 2.36 119,78 Kuadrat Tengah 23,48 0,13 Hit 180,61** F 0,05 2,77 0,01 4,25

Keterangan : * berarti berbeda nyata pada = 0,05

Perhitungan Jumlah Kuadrat (JK)


GT2 (963,9)2 FK = ---------- = ------------ = 38712,63 txr 6x4

JK Total = ( Yij2 FK = 43,12 + + 37,72 + 36,52 FK = 119,78 43,12 + 36,922 JK. Perl. = ( Y, J2 FK = -------------------------- - FK = 117,42 4 JK Galat = JK total JK Perlakuan = 119,78-117,42 = 2,36
UJI LANJUT: UJI BEDA RATA-RATA UJI LSD: - Fhit > Ftab - Harus ditetapkan pembanding (kontrol) Sd = (2(KT galat)/r = 2(0,13)/4=0,25 LSD = t/2, db galat x Sd = 2,10 x 0,25 = 0,53 UJI DUNCAN (LSR) LSR = SSR x Sx Sx = KT galat/r = 0,18 LSR = SSR x Sx

P 2 SSR 2,97 LSR 0,53


Rata2 Perlakuan F=36,92 a E=37,92 b D=39,70 c C=41,15 d B=42,17 e A=43,10 f

3 4 5 6 3,12 3,21 3,27 3,32 0,56 0,57 0,58 0,60


Beda dua rata-rata 1 2,78 4,23 5,25 6,18 1,78 3,23 4,25 5,18 1,45 2,47 3,40 1,02 1,95 0,93 LSD 0,53 0,53 0,56 0,57 0,59 0,60 LSR

KESIMPULAN PENELITIAN
Perlakuan A yaitu lamanya Blensing selama 3

menit merupakan perlakuan yang paling baik dan berbeda nyata dengan perlakuan lainnya Perlakuan F yaitu lamnaya blensing selama 18 menit merupakan perlakuan yang paling jelek dan berbeda nyata dengan perlakuan lainnya Semakin pendek waktu blensing semakin baik kualitas pepaya.

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