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BIOREMED

IATION
SUBMITTED TO- DR.DEEPKA
SUBMITTED BY- AKANSHA THAKUR
KOMAL SHARMA
VISHALI
WHAT IS BIOREMEDIATION?
 Bioremediation is the use of organisms(primarily
microorganisms) for removal of pollutants from biosphere .
 It relies on biological processes to minimize
unwanted environmental impact on environment.
 The microorganisms have the ability to degrade,
detoxify and even accumulate harmful organisms and
inorganic compounds.
• It is the most ideal alternative, environment friendly
and ecologically sound technology for removing
pollutant from the environment.
• Bioremediation infact forms a vital component of the
so called GREEN MOVEMENT of maintaining the
nature’s overall ecological balance.
BIOREMEDIATION
EFFECTIVENESS
 Depends on:
• Microorganisms
• Environmental factors
• Contaminant type & state
MICROORGANISMS
 Aerobic bacteria-
• Examples include Pseudomonas, Alcaligenes,
Sphingomonas, Rhodococcus &Mycobacterium.
• Shown to degrade pesticides and hydrocarbons.
• May be able to use the contaminant as sole
source of carbon and energy.
 Methanotrophs-
• Aerobic bacteria that utilise methane for carbon
and energy.
• Active against a wide range of compounds
 Anaerobic bacteria-
• Not used as frequently as aerobic bacteria.
• Can often be applied to bioremediation of
ploychlorinated biphenyls(PCBs) in river
sediments.
 Fungi-
• Able to degrade a diverse range of persistent or
toxic environmental pollutants.
MICROORGANISMS CAN LIVE AT
DIFFERENT PH CONDITIONS
MICROORGANISMS CAN LIVE AT
ANY TEMPERATURE CONDITIONS
ENVIRONMENTAL FACTORS
TYPES OF BIOREMEDIATION:
A. In-situ bioremediation
B. Ex-situ bioremediation

 IN-SITU BIOREMEDIATION: It is applied to eliminate the


pollutants in contaminated soils and ground water.
• It is a superior method for cleaning of contaminated
environments because it saves transportation costs.
• Uses harmless microorganisms to eliminate the chemical
contaminants.
• Causes least disruption of the contaminated area.
• The method is time consuming and it leads to change in
seasonal variations in the microbial activity.
• There are further two types of in-situ bioremediation:
1. INTRINSIC BIOREMEDIATION- It uses microorganisms already present
in the environment to biodegrade harmful contaminant. There is no
human intervention involved in this type of bioremediation.
2. It is the cheapest means of bioremediation available, it is the most
commonly used.

3. ENGINEERED IN-SITU BIOREMEDIATION- This type of bioremediation


is performed through the introduction of certain microorganisms to
a contamination site.
4. It accelerates the degradation process by enhancing the
physiochemical conditions to encourage the growth of
microorganisms.
5. Oxygen, electron acceptors and nutrients promote microbial
growth.

EX-SITU BIOREMEDIATION: The process of bioremediation here takes


place somewhere out from contamination site and requires
transportation of contaminated soil or pumping of groundwater to
the site of bioremediation.

• SOLID PHASE SYSTEM(including land treatment and soil piles) the


system is use
• EX-SITU BIOREMEDIATION: The process of bioremediation here takes place
somewhere out from contamination site and requires transportation of
contaminated soil or pumping of groundwater to the site of
bioremediation.
 SOLID PHASE SYSTEM EX SITU BIOREMEDIATION:
• COMPOSTING ia a technique that involves combining contaminated soil
with organic compounds such as agricultural wastes.
• The presence of these organic materials supports the development of a
rich microbial population and the elevated temperature characteristic of
composting.
• LAND FARMING OPERATION:
• Land farming is a simple technique in which contaminated soil is
excavated and spread over a prepared bed and periodically tilled until
pollutants are degraded. The practice is limited to the treatment of
superficial 10-35 cm of soil.
• BIOPILE SYSTEM:
• Biopiles are a hybrid of land farming and composting. Essentially,
eginnered cells are constructed as aerated composted piles. Biopiles
provide a favourable environment for indigenous areobic and anareobic
microorganisms.
APPROACHES TO
BIOREMEDIATION
 There are three approaches to bioremediation:
1. Biostimulation
2. Bioaugmentation
3. Intrinsic bioremediation

 BIOSTIMULATION: It depends primarily on modification of


environment to stimulate existing bacteria capable of
bioremediation.
 This can be done by addition of various forms of rate limiting
nutrients and electron acceptors, such as phosphorus, nitrogen,
oxygen or carbon.
 BIOAUGMENTATION: It uses addition of microbial culture to increase
biodegradation of a contaminant.
 It is commonly used in municipal wastewater treatment to restart
activated sludge bioreactors.
 INTRINSIC BIOREMEDIATION: It manages the innate
capabilities of naturally occurring microbes to degrade
contaminants without taking any engineering step to
enhance the process.
 Also called Natural attenuation.
USE OF FUNGI IN
BIOREMEDIATION
 White rot fungi can degrade organic pollutants in soil and
effluent and decolorize Kraft black liquor, e.g.
Phanerochaete chrysosporium can produce aromatic
mixtures with its lignolytic system.
 Pentachlorophenol, even TNT can be degraded by white
rot fungi.
USE OF BACTERIA IN
BIOREMEDIATION
 These microbes are usually thermophillic anaerobic.
 They contain monooxygenases and dehydrogenases to
break down organic matter including toxic substances.
 Genetically engineered bacteria( Pseudomonas) with
plasmid producing enzymes to degrade octane and many
different organic compounds from crude oil.
CASE STUDY: OIL DEGRADATION
 Oil metabolising bacteria were known to exist, but
when introduced into an oil spill, competed with
each other, limiting the amount of crude oil that
they degraded.
 Prof. Chakrabarty discovered a method for genetic
cross-linking that fixed all four plasmid genes in
place and produced a new, stable, bacteria species
(now called Pseudomonas putida) capable of
consuming oil one or two orders of magnitude faster
than the previous four strains of oil eating microbes.
 The new microbe, which Chakrabarty called “multi-
plasmid hydrocarbon-degrading Pseudomonas,”
could digest abot two thirds of the hydrocarbons
that would be found in a typical oil spill.

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