Biological Oxidation

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BIOLOGICAL OXIDATION

Oxidation:
• Transfer of electron (negative charge) from one compound to other.

• All biological reactions involve electron flow. Here, one reactant is oxidized (loses electron) and
the other is reduced (gains electron).

• This couple of reaction that deal with the loss and gain of electron are called REDOX reactions.

Biological oxidation :
• It is the reaction in the living cells that is meant for the purpose of energy production. It comes
coupled with reduction reaction.

• It utilizes multiple steps to synthesize ATP.

• Also known as Biological oxidation-reduction reaction OR Redox reaction.


BIOLOGICAL OXIDATION
Enzymes :

• Enzymes in redox reactions are known as oxidoreductases.

• They are classified into 4 groups


• Oxidases
• Dehydrogenases
• Hydroperoxidases
• Oxygenases
BIOLOGICAL OXIDATION
OXIDASES
• Use oxygen as hydrogen acceptor
• Removes hydrogen from a substrate and add that hydrogen to a oxygen
• End product is usually H2O or H2O2
• Required during aerobic processes
• eg. Cytochrome oxidase, xanthine oxidase, etc

DEHYDROGENASES
• Functional during anaerobic process
• Does not utilize oxygen as hydrogen acceptor
• May utilize co-enzymes as hydrogen carriers
• eg. NAD, FMN, FAD, etc
BIOLOGICAL OXIDATION
HYDROPEROXIDASES
• Catalyses the reduction of H2O2 to H2O.
• Play a pivotal role in oxidative stress
• Contains a heme bound to the protein usually through histidine
residue (ex. Glutathione peroxidase)
• Eg. Alkyl hydroperoxidase, prostaglandin hydroperoxidase.

OXYGENASES
• Catalyses the addition of oxygen in the molecules without
energy production.
• Two types
• Monooxygenases – inserts one atom of oxygen
• Dioxygenases - inserts two atoms of oxygen
BIOLOGICAL OXIDATION
Cytochromes
• Are heme containing redox proteins
having a central iron atom as its cofactor
• Classified according to the type of heme
and its bond
• Cytochrome a
• Cytochrome b
• Cytochrome c
• Cytochrome d
BIOLOGICAL OXIDATION
Cytochromes
• Are mainly seen in mitochondria
• Are capable of redox reactions with the
help of iron atom
• Iron present in heme can stay in ferric
or ferrous state.
• These iron atoms can either take up or
lose electrons and help in electron
transfer reactions

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