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    Microwave Spectros

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    18BS701003 bsc.physics.18
    Microwave spectroscopy can be used to measure bond lengths and observe translating stereochemistries through precise spectral observations. It is also used to detect stable molecules present in interstellar clouds through radio telescope observations. The Stark effect causes shifting and splitting of spectral lines when an external electric field is present, and can provide information about molecular shape. Microwave spectroscopy allows for quantitative chemical analysis by detecting whole molecules and isotopes, and distinguishing isomers.

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    Microwave Spectros

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    18BS701003 bsc.physics.18
    22 views52 pages
    Microwave spectroscopy can be used to measure bond lengths and observe translating stereochemistries through precise spectral observations. It is also used to detect stable molecules present in interstellar clouds through radio telescope observations. The Stark effect causes shifting and splitting of spectral lines when an external electric field is present, and can provide information about molecular shape. Microwave spectroscopy allows for quantitative chemical analysis by detecting whole molecules and isotopes, and distinguishing isomers.

    Original Title

    MICROWAVE SPECTROSCOPY

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    Microwave spectroscopy can be used to measure bond lengths and observe translating stereochemistries through precise spectral observations. It is also used to detect stable molecules present in interstellar clouds through radio telescope observations. The Stark effect causes shifting and splitting of spectral lines when an external electric field is present, and can provide information about molecular shape. Microwave spectroscopy allows for quantitative chemical analysis by detecting whole molecules and isotopes, and distinguishing isomers.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    22 views52 pages

    Microwave Spectros

    Uploaded by

    18BS701003 bsc.physics.18
    Microwave spectroscopy can be used to measure bond lengths and observe translating stereochemistries through precise spectral observations. It is also used to detect stable molecules present in interstellar clouds through radio telescope observations. The Stark effect causes shifting and splitting of spectral lines when an external electric field is present, and can provide information about molecular shape. Microwave spectroscopy allows for quantitative chemical analysis by detecting whole molecules and isotopes, and distinguishing isomers.

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    © All Rights Reserved
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    Download as pptx, pdf, or txt
    of 52

    MICROWAVE SPECTROSCOPY

    Syllabus
    • Unit I: Light scattering and Electron Microscopy Elastic and inelastic scattering,
    light scattering by macromolecules, dynamic light scattering, radius of gyration and
    molecular mass. Transmission and scanning microscopy, negative staining, cryo-
    electron microscopy.
    • Unit II: Chromatography and Mass spectrometry Electrospray MS, MALDI,
    applications. Paper Chromatography, TLC, column, gas-liquid, ion-exchange, size-
    exclusion and affinity chromatographies, HPLC and FPLC, applications to
    macromolecules.
    • Unit: III: IR and Raman spectroscopy Rotational and vibrational spectra, oscillator,
    molecular symmetry, optical density, investigations of molecular structure,
    hydrogen bonding. Examples and comparison of IR and Raman spectra, resonance
    Raman spectroscopy
    • Unit IV: Absorption, Fluorescence and NMR Spectroscopy UV and Visible spectra,
    chromophores, Circular Dichroism and Optical Rotatory Dispersion, cotton effect,
    applications to proteins and nucleic acids, Frank-condon principle, classical
    picture, resonance condition, Bloch condition, relaxation phenomenon, Fourier
    transform technique. NMR, chemical shifts, coupling constraints, Karplus
    equation, analysis of simple NMR spectra, Nuclear Overhauser Effect, proton
    magnetic resonance, 13C and 31P spectra.
    Sample Preparation
    • Gas samples
    –As is
    –As a diluted analyte in a non-MW reactive gas
    such as Neon
    • Solids
    –Laser abatement
    –Vaporization
    • Liquids
    –Vaporization
    –Supersonic Expansion
    Applications of MW

    •Measurement of bond lengths


    •Observation by radio telescopes for life precursors in
    interstellar clouds
    •Precise observation of translating stereochemistries
    and confirmation verification
    Stark Effect
    Stark effect is shifting and splitting of spectral
    lines of atoms and molecules due to the
    presence of an external electric field.

    For most spectral lines, Stark effect is either


    linear or quadratic with a high accuracy.

    For Rotational Spectroscopy, one uses a


    perpendicular electric field.
    Stark Effect
    • The electric field interacts with the molecular
    dipole moment, and there is a shift in the
    position of the lines
    • For a linear molecule
    Δν = (μE)2
    A method to
    • For a Symmetric Top Molecule find the shape
    of the molecule.
    Δν = μE
    Chemical Analysis
    • Whole molecule is involved.
    • Process is quantitative
    • Isotopes can be detected
    • Can detect isomers with different moment of
    intertia
    • Used to detect stable molecules in space

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