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    Mass Balance-Exercise

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    hmoss
    1) A continuous fermentation process is proposed to produce 2 kg/h of acetic acid from ethanol using immobilized Acetobacter aceti cells. 2) Air is pumped in at 200 gmol/h to support the aerobic conversion of ethanol to acetic acid. 3) The minimum amount of ethanol required and water needed to dilute the ethanol to avoid inhibiting the cells with more than 12% acetic acid concentration must be calculated.

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    Mass Balance-Exercise

    Uploaded by

    hmoss
    29 views19 pages
    1) A continuous fermentation process is proposed to produce 2 kg/h of acetic acid from ethanol using immobilized Acetobacter aceti cells. 2) Air is pumped in at 200 gmol/h to support the aerobic conversion of ethanol to acetic acid. 3) The minimum amount of ethanol required and water needed to dilute the ethanol to avoid inhibiting the cells with more than 12% acetic acid concentration must be calculated.

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    Mass balance-exercise

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    1) A continuous fermentation process is proposed to produce 2 kg/h of acetic acid from ethanol using immobilized Acetobacter aceti cells. 2) Air is pumped in at 200 gmol/h to support the aerobic conversion of ethanol to acetic acid. 3) The minimum amount of ethanol required and water needed to dilute the ethanol to avoid inhibiting the cells with more than 12% acetic acid concentration must be calculated.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    29 views19 pages

    Mass Balance-Exercise

    Uploaded by

    hmoss
    1) A continuous fermentation process is proposed to produce 2 kg/h of acetic acid from ethanol using immobilized Acetobacter aceti cells. 2) Air is pumped in at 200 gmol/h to support the aerobic conversion of ethanol to acetic acid. 3) The minimum amount of ethanol required and water needed to dilute the ethanol to avoid inhibiting the cells with more than 12% acetic acid concentration must be calculated.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
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    Mass balance

    Tutorial
    CONTINUOUS ACETIC ACID FERMENTATION

    Acetobacter aceti bacteria convert ethanol to


    acetic acid under aerobic conditions. A
    continuous fermentation process for vinegar
    production is proposed using nongrowing A. aceti
    cells immobilised on the surface of gelatin beads.
    Air is pumped into the fermenter at a rate of
    200gmol h-1. The production target is 2 kgh-1 acetic
    acid and the maximum acetic acid concentration
    tolerated by the cells is 12%.
    Calculate:
    1) What minimum amount of ethanol is
    required?
    2) What minimum amount of water must be
    used to dilute ethanol to avoid acid
    inhibition?
    Solution:
    1) Draw a flow sheet for this process.
    -system, system boundary and stream (input +
    output)
    2) Analyse
    • Assumptions
    -steady state
    -no leaks
    -Inlet air is dry
    -gas volume % = mole%
    -no evaporation of ethanol, water or acetic acid
    -complete conversion of ethanol
    -ethanol is used by the cells for synthesis of acetic acid only; no side-
    reactions occur
    Extra data
    • Molecular weights:
    -ethanol = 46
    -acetic acid = 60
    - O2 = 32
    - N2 = 28
    - H2O = 18
    - Composition of air 21% O2, 79% N2
    Input
    stream Ethanol Acetic H2O O2 N2 Total
    acid
    Feed stream E 0 W 0 0 E+W
    Inlet air 0 0 0 1.344 4.424 5.768
    Product - - - - - -
    stream
    Off-gas - - - - - -
    Total E 0 W 1.344 4.424 5.768 + E
    +W
    Output
    stream Ethanol Acetic H2O O2 N2 Total
    acid
    Feed stream - - - - - -

    Inlet air - - - - - -

    Product 0 2 14.67 0 0 16.67


    stream
    Off-gas 0 0 0 ? ? G

    Total 0 2 14.67 ? ? 16.67 + G

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