Physiology of the Pleural Space

Visceral Pleura
• Covers the lung parenchyma including the
interlobar fissures.
• Provides mechanical support to the lung.
• Limits lung expansion, protecting the lung .
• Contributes to the elastic recoil of the lung
and lung deflation.
 Visceral Pleura
• Thick visceral pleura: mesothelium and dense
layer of connective tissue.
• Systemic circulation. Bronchial arteries.
• Blood, lymph vessels and nerves.
• Humans, sheep, cows, pigs and horses.
• Thin visceral pleura: monkeys, dogs and cats.
• Pulmonary circulation.
 Pleural Visceral
• Mean thickness:25-83 um.
• Distance from the microvessels to the pleura:
18-56um.
• Drainage into the pulmonary veins.
 Parietal Pleura
• Lines the inside of the thoracic cavities.
• Subdivided into the costal, mediastinal and
diaphragmatic parietal pleura.
• Loose connective tissue and single layer of
mesothelial cells.
• Capillaries and lymphatic lacunas.
• Blood supply from systemic capillaries.
• Blood drainage: Intercostal veins.
 Parietal Pleura
• Mean thickness : 20-25 um.
• Distance from the micro vessel to the pleural
space: 10-12 um.
• Stomas communicates lymphatic vessels with
pleural space.
• Nitric oxide.
• Diaphragmatic pleura.
Stomas and Valves of the Parietal
Pleura
 Parietal Pleura
• Lymphatic lacunas are in communication with
the pleural space by stomas and ultimately
drain to the internal mammary, para aortic
and diaphragmatic lymph nodes.
 Mesothelial Cells
• Very active cells.
• Mesothelium. Dynamic cellular membrane.
• Transport and movement of fluid and
particulate matter.
• Leukocyte migration.
• Synthesis of cytokines, growth factors and
extracellular proteins.
 Mesothelial Cells
• Mesothelial cell can convert to macrophages
and myofibroblasts.
• TGF Beta.
• Microvilli: entangle glycoproteins rich in
hyaluronic acid.
 Pleural Fluid
• 8.4 +/- 4.3 mL.
• Total pleural fluid volume: 0.26 +/- 0.1 mL/Kg.
• Cell count: WBC: 1716x103 cells/ml. RBC:
700x103 cells/ml.
• Macrophages:75%.
• Lymphocytes: 23%
• Mesothelial cells, neutrophils and
eosinophils:2%.
 Pleural Pressure
• Negative pressure generated between the
visceral and parietal pleura by the opposing
elastic forces of the chest wall and lung at FRC.
• Represents the balance between the outward
pull of the thoracic cavity and the inward pull
of the lung.
 Pleural Pressure
• It is the pressure at the outer surface of the
lung and the heart and inner surface of the
thoracic cavity.
• Distensible structures.
• Compliance and pressure difference between
inside and outside.
• Primary determinant of the lung, cardiac and
thoracic cavity volume.
 Measurement
• Indirect measurement. Esophageal pressure.
• Lower one third of the esophagus.
• Upright posture.
• Analysis of lung and chest wall compliance,
work of breathing, respiratory muscle function
and the presence of diaphragm paralysis.
• Mechanical ventilation guided by esophageal
pressures in ALI. November 13, 2008.
 Pleural Pressure
• Pleural pressure is not uniform.
• Gradient between the superior ( lowest, most
negative) and inferior (highest, least negative)
portions of the lung.
• 0.3 cm H20/ cm vertical distance.
• In the upright position, gradient of pleural
pressure between the apex and the base is
approximately 8 cm H20.
 Pleural Pressure
• Gravity.
• Mismatching of the shapes of the lung and
chest wall.
• The weight of the lung and other intra
thoracic structures.
• Alveolar pressure is constant throughout the
lung.
• Differents parts of the lung have different
distending pressures.
 Pleural Pressure
• Different parts of the lungs have different
distending pressures.
• The alveoli in the superior parts of the lung
tends to be larger than those in the inferior
parts.
• Formation of pleural blebs.
• Uneven distribution of ventilation.
 Pleural fluid formation
• Pleural capillaries.
• Interstitial space in the lung.
• Intra thoracic blood vessels. Hemothorax.
• Intra thoracic lymphatics. Chylothorax.
• Peritoneal cavity.
 Starling’s Law of Trans capillary
Exchange
Qf = Lp x A[(Pcap-Ppl) – σd(πcap-πpl)]

Qf = liquid movement
Lp = filtration coefficient /unit area of the membrane
A = surface area of the membrane
σd = solute reflection coefficient for protein
(membrane's ability to restrict passage of large
molecules
P = hydrostatic pressures
π = oncotic pressure
 Pleural Capillaries
• A gradient for fluid formation is normally
present in the parietal pleura.
• Hydrostatic pressure: 30cm H20.
• Pleural Pressure: -5 cm H20.
• Oncotic pressure in plasma: 34 cm H20.
• Oncotic pressure in the pleural fluid: 5 cm
H20.
• Net pressure gradient: 6 cm H20.
 Pleural Capillaries
• Net gradient: close to zero.
• Pleural visceral capillaries drain into the
pulmonary veins.
• The filtration coefficient is substantially less
than for the parietal pleura.
 Interstitial origin
• Much of the pleural fluid.
• High pressure pulmonary edema: the pleural
fluid formed is directly related to the elevation
in the wedge pressure.
• Increases in pleural fluid formation occurs
only after the development of pulmonary
edema.
• The presence of pulmonary effusion is more
closely correlated with the pulmonary venous
pressure than with the systemic venous
pressure.
• High permeability pulmonary edema: Pleural
fluid accumulates only after pulmonary
edema develops.
• In general : pleural effusion develops when
the extravascular lung water has reached a
critical level in a certain amount of time.
• 5-8 g of fluid/ gram of dry lung.
• Increasing levels of interstitial fluid is related
with increase in sub pleural interstitial
pressure, allowing fluid transverse the visceral
pleura to the pleural space.
• Pressure gradient rise from 1.3 to 4.4 cm H2O.
• Associated to rise in lung water to 5-6 g/g dry
lung.
 Peritoneal Cavity
• Free fluid in the peritoneal cavity.
• Opening in the diaphragm. Diaphragmatic
defects.
• Pressure in the pleural cavity is less than the
pressure in the peritoneal cavity.
 Pleural Fluid Absorption
• Mean lymphatic flow is 0.22-0.4 mL/kg/hour.
• Lymphatics operate at maximum capacity
once the volume of the pleural liquid exceeds
a certain threshold.
• The capacity for lymphatic clearance is 28
times as high as the normal rate of pleural
fluid formation.
 Pathogenesis of Pleural Effusion
• Pleural fluid formation exceeds the rate of
pleural fluid absorption.
Clinical Implications of
Pneumothorax
 Effects of Pneumothorax on Pleural
Pressure
• Air will flow into the pleural space until a
pressure gradient no longer exits or until the
communication is sealed.
 Effects of Pneumothorax on Pleural
Pressure
• The distribution in the increase in the pleural
pressure is homogenous and the pressure is
the same throughout the entire pleural space.

• In pleural effusion there is a gradient in the

pleural pressure due to the hydrostatic
column of fluid.
 Effects of Pneumothorax on Pleural
Pressure
• The upper lobe is affected more than the
lower lobe in pneumothorax, because the
pressure in the apices is much more negative
that at the bases.
 Effects of Pneumothorax in Lung
Function
• Restrictive ventilatory defect.
• Decreased Vital Capacity.
• Decreased FRC.
• Decreased TLC.
• Decreased RV.
• Decreased end expiratory lung volume.
• Increased end expiratory thoracic volume?.
• Slightly decreased DLCO.
 Effects of Pneumothorax in Lung
Function
• Expansion of the pleural space is
accommodated partly by deflation of the lung
and partly by relative expansion of the
ipsilateral chest wall.
 Effects of Pneumothorax in Blood
Gases
• Reduction of PaO2 : anatomic shunts and
areas of low ventilation perfusion ratios.
• Increased A-a oxygen difference.
 Effects of Pneumothorax in Blood
Gases
• The more extensive the pneumothorax the
lower is the arterial oxygen tension and the
larger the anatomical shunt.
Anatomical Shunt and Lung Area Index
 Effects of Pneumothorax in Blood
Gases
• Complete non-ventilation of alveoli does not
occur in man until the lung volume is reduced
to about 65% of normal.
• Increase in A-aD02 is associated to anatomical
shunt and decrease ventilation perfusion
disturbances.
After Drainage of Pneumothorax
 Effects of Pneumothorax in Blood
Gases
• The distribution of ventilation-perfusion ratios
could became more uneven and could cause a
rise in either A-aDo2 or VD/VT.
• Relatively great pressure is required to open a
ventilatory unit which is completely closed.
 Effects of Pneumothorax in Blood
Gases
• Persistent alveolar collapse and
bronchoconstriction (serotonin and
histamine).
• Persistent alveolar hypoxemia will cause local
vasoconstriction and under perfusion.
 Effects of Pneumothorax in Cardiac
Function
• Tension pneumothorax is associated to
impaired hemodynamics.
• Decreased CO and MSP.
• Decreased cardiac output secondary to
decrease venous return due to the increased
pleural pressures.
 Effects of Pneumothorax in Cardiac
Function
• Cardiovascular collapse in response to tension
pneumothorax is preceded by and likely
caused by respiratory failure resulting in
profound hypoxemia, hypercarbia, and
subsequent acidemia.
 Circulatory changes associated with
Tension Pneumothorax
• Gradual increase in pressure thought out the
system without significant gradients.
• Cardiac output and systemic arterial pressure
did not drop in association with rising
pressures in the right side of the circulation.
 Circulatory changes associated with
Tension Pneumothorax
• All low pressure vascular elements within the
thorax are affected by rising intra thoracic
pressure.
 Ventilatory changes associated with
Tension Pneumothorax
• The ipsilateral pleural pressure become
progressively less negative until become
positive.
• The contralateral pleural pressure will become
progressively more negative.
• The respiratory excursion will become wider,
reflecting increased respiratory effort.
• The mediastinum was the most significant
factor behind this compensatory mechanism.
• Depression of respiratory system by
hypoxemia.
• Worsening tensional pneumothorax
associated to worsening in shunting.
 References
• Pleural Diseases. Richard W. Light.
• Assessment of Pleural Pressure in the Evaluation of Pleural Effusion. David
Feller-Kopman. Chest 2009;135;201-209
• Changes in bronchial and pulmonary arterial blood flow with progressive
tension pneumothorax. Paula Carvalho. J Appl Physiol 81:1664-1669, 1996.
• Effects-of pneumothorax or pleural effusion on pulmonary function. JJ
Gilmartin. Thorax 1985;40:60-65