CELL & ITS FUNCTIONS

DR. AMBER TARIQ

MS-NMPT (RIU)
DPT (UOL)
ORGANIZATION OF CELL
 Nucleus & cytoplasm
 The nucleus is separated from the cytoplasm by a nuclear membrane,
and the cytoplasm is separated from the surrounding fluids by a cell
membrane, also called the plasma membrane.
 The different substances that make up the cell are collectively called
protoplasm.
 Protoplasm is composed mainly of five basic substances: water,
electrolytes, proteins, lipids, and carbohydrates.
 Water: 70-85%
 Ions: potassium, magnesium, phosphate, sulfate, bicarbonate, and
smaller quantities of sodium, chloride, and calcium. Helps in
transmission of electrochemical impulses in nerve and muscle fibers.
 Proteins: 20%. Two types: structural proteins and functional
proteins.
 Structural proteins are present in the cell mainly in the form of long
filaments that are polymers of many individual protein molecules.
Intracellular filaments form microtubules that provide the
“cytoskeletons” of such cellular organelles as cilia, nerve axons and a
tangled mass of thin filamentous tubules that hold the parts of the
cytoplasm and nucleoplasm together in their respective compartments.
Extracellularly, fibrillar proteins are found especially in the collagen and
elastin fibers of connective tissue and in blood vessel walls, tendons,
ligaments, and so forth.
 Functional proteins are an entirely different type of protein, usually
composed of combinations of a few molecules in tubular-globular form.
These proteins are mainly the enzymes of the cell and, in contrast to the
fibrillar proteins, are often mobile in the cell fluid. The enzymes come
into direct contact with other substances in the cell fluid and thereby
catalyze specific intracellular chemical reactions.
 Lipids: Substances that are grouped together because of their
common property of being soluble in fat solvents e.g. phospholipids
and cholesterol (2% of total mass). They are mainly insoluble in water
and, therefore, are used to form the cell membrane and intracellular
membrane barriers that separate the different cell compartments.
Some cells contain large quantities of triglycerides, also called
neutral fat. In the fat cells, triglycerides often account for as much as
95% of the cell mass. The fat stored in these cells represents the
body’s main storehouse of energy-giving nutrients that can later be
dissoluted and used to provide energy wherever in the body it is
needed.
 Carbohydrates: Little structural function in the cell, major role in
nutrition of the cell. 3% in muscle cells, 6% in liver cells. A small
amount of carbohydrate is stored in the cells in the form of glycogen,
which is an insoluble polymer of glucose that can be depolymerized
and used rapidly to supply the cells’ energy needs.
PHYSICAL STRUCTURE OF THE CELL
 Intracellular organelles such as mitochondria
 Membranous Structures of the Cell:
 Cell membrane, nuclear membrane, membrane of the endoplasmic
reticulum, and membranes of the mitochondria, lysosomes, and Golgi
apparatus.
 Cell Membrane:
 Envelops the cell, is a thin, pliable, elastic structure only 7.5 to 10
nanometers thick.
 Composed almost entirely of proteins and lipids.
 The approximate composition is proteins 55 % , phospholipids 25 % ,
cholesterol 13 %, other lipids 4 % , and carbohydrates 3 % .
 Lipid Barrier of the Cell Membrane Impedes Water Penetration:
 Lipid bilayer, which is a thin, double-layered film of lipids—each layer only
one molecule thick—that is continuous over the entire cell surface.
Interspersed in this lipid film are large globular protein molecules.
 Composed of phospholipid molecules. One end of each phospholipid
molecule is soluble in water; that is, it is hydrophilic. The other end is
soluble only in fats; that is, it is hydrophobic. The phosphate end of the
phospholipid is hydrophilic, and the fatty acid portion is hydrophobic.
 Because the hydrophobic portions of the phospholipid molecules are
repelled by water but are mutually attracted to one another, they have a
natural tendency to attach to one another in the middle of the membrane.
 The hydrophilic phosphate portions then constitute the two surfaces of the
complete cell membrane, in contact with intracellular water on the inside
of the membrane and extracellular water on the outside surface.
 The lipid layer in the middle of the membrane is impermeable to the usual
water-soluble substances, such as ions, glucose, and urea. Conversely, fat-
soluble substances, such as oxygen, carbon dioxide, and alcohol, can
penetrate this portion of the membrane with ease.
 Integral and Peripheral Cell Membrane Proteins:
 Integral proteins that protrude all the way through the membrane and
peripheral proteins that are attached only to one surface of the membrane
and do not penetrate all the way through.
 Integral proteins provide structural channels (or pores) through which water
molecules and water soluble substances, especially ions, can diffuse
between the extracellular and intracellular fluids (also have selective
properties that allow preferential diffusion of some substances over others).
Other integral proteins act as carrier proteins for transporting substances
that otherwise could not penetrate the lipid bilayer. Sometimes these even
transport substances in the direction opposite to their electrochemical
gradients for diffusion, which is called “active transport.” Others act as
enzymes.
 Also serve as receptors for water-soluble chemicals, such as peptide
hormones, that do not easily penetrate the cell membrane.
 Also acts as a ligand that bind to the receptor causes conformational changes
in the receptor protein. This, in turn, enzymatically activates the intracellular
part of the protein or induces interactions between the receptor and proteins
in the cytoplasm that act as second messengers, thereby relaying the signal
from the extracellular part of the receptor to the interior of the cell. In this
way, integral proteins spanning the cell membrane provide a means of
conveying information about the environment to the cell interior.
 Peripheral protein molecules are often attached to the integral proteins.
These peripheral proteins function almost entirely as enzymes or as controllers
of transport of substances through the cell membrane “pores.”
 Membrane Carbohydrates—The Cell “Glycocalyx”:
 Carbohydrates occur mostly in combination with proteins or lipids in the form
of glycoproteins or glycolipids. Most of the integral proteins are
glycoproteins, and about one tenth of the membrane lipid molecules are
glycolipids.
 “Glyco” portions of molecules almost protrude to the outside of the cell,
dangling outward from the cell surface.
 Carbohydrate compounds, called proteoglycans— which are mainly
carbohydrate substances bound to small protein cores—are loosely attached
to the outer surface of the cell as well. Thus, the entire outside surface of
the cell often has a loose carbohydrate coat called the glycocalyx.
 Properties of carbohydrate moieties:
 (1) Many of them have a negative electrical charge, which gives most cells an
overall negative surface charge that repels other negative objects.
 (2) The glycocalyx of some cells attaches to the glycocalyx of other cells,
thus attaching cells to one another.
 (3) Many of the carbohydrates act as receptor substances for binding
hormones, such as insulin; when bound, this combination activates attached
internal proteins that, in turn, activate a cascade of intracellular enzymes.
 (4) Some carbohydrate moieties enter into immune reactions.
CYTOPLASM AND ITS ORGANELLES
 Clear fluid portion is called cytosol, contains mainly dissolved proteins,
electrolytes, and glucose.
 Dispersed in the cytoplasm are neutral fat globules, glycogen granules,
ribosomes, secretory vesicles, and five especially important organelles: the
endoplasmic reticulum, the Golgi apparatus, mitochondria, lysosomes, and
peroxisomes.
 Endoplasmic reticulum:
 Network of tubular and flat vesicular structures in the cytoplasm; this is the
endoplasmic reticulum.
 The tubules and vesicles interconnect with one another. Also, their walls are
constructed of lipid bilayer membranes that contain large amounts of
proteins, similar to the cell membrane. The total surface area of this
structure in some cells—the liver cells, for instance—can be as much as 30 to
40 times the cell membrane area.
 The space inside the tubules and vesicles is filled with endoplasmic matrix.