Chromatographic Analyzers
Chromatographic Analyzers
Chromatographic Analyzers
Allows the use of very much smaller particle size for the column
packing material which gives a much greater surface area for
interactions between the stationary phase and the molecules flowing
past it resulting to a better separation of the components of the mixture
How does it work?
A reservoir holds the solvent (mobile phase) while a high-pressure
pump is used to generate and meter a specified flow rate (typically in
mL/min) of mobile phase. On the other hand, an injector inject the
sample into the continuously flowing mobile phase stream that carries
the sample into the HPLC column. The column contains the
chromatographic packing material needed to effect the separation.
This packing material is called the stationary phase because it is held
in place by the column hardware. Also, a detector is needed to
detect the separated compound bands as they elute from the HPLC
column. Afterwards, the mobile phase exits the detector and can be
sent to waste, or collected, as desired. Additionally, the detector is
wired to the computer data station which records the electrical signal
needed to generate the chromatogram on its display and to identify
and quantitate the concentration of the sample constituents.
2 Basic Elution Modes in HPLC
Isocratic Elution - the mobile phase (either a pure solvent
or a mixture) remains the same throughout the run.