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    Protein in Other Fluids

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    Mustafa Khandgawi
    This document discusses proteins found in various body fluids other than blood. It describes that urinary proteins can indicate issues like proteinuria. CSF proteins are normally 15-45 mg/dl and increased levels can be caused by meningitis, MS, or tumors. CSF protein analysis involves measuring albumin and IgG to determine if increased permeability or local production is the cause. Electrophoresis of CSF from MS patients shows distinct oligoclonal bands. Other body fluids discussed include amniotic fluid, peritoneal fluid, and pleural fluid.

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    Protein in Other Fluids

    Uploaded by

    Mustafa Khandgawi
    23 views14 pages
    This document discusses proteins found in various body fluids other than blood. It describes that urinary proteins can indicate issues like proteinuria. CSF proteins are normally 15-45 mg/dl and increased levels can be caused by meningitis, MS, or tumors. CSF protein analysis involves measuring albumin and IgG to determine if increased permeability or local production is the cause. Electrophoresis of CSF from MS patients shows distinct oligoclonal bands. Other body fluids discussed include amniotic fluid, peritoneal fluid, and pleural fluid.

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    Protein in Other Fluids

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    This document discusses proteins found in various body fluids other than blood. It describes that urinary proteins can indicate issues like proteinuria. CSF proteins are normally 15-45 mg/dl and increased levels can be caused by meningitis, MS, or tumors. CSF protein analysis involves measuring albumin and IgG to determine if increased permeability or local production is the cause. Electrophoresis of CSF from MS patients shows distinct oligoclonal bands. Other body fluids discussed include amniotic fluid, peritoneal fluid, and pleural fluid.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    23 views14 pages

    Protein in Other Fluids

    Uploaded by

    Mustafa Khandgawi
    This document discusses proteins found in various body fluids other than blood. It describes that urinary proteins can indicate issues like proteinuria. CSF proteins are normally 15-45 mg/dl and increased levels can be caused by meningitis, MS, or tumors. CSF protein analysis involves measuring albumin and IgG to determine if increased permeability or local production is the cause. Electrophoresis of CSF from MS patients shows distinct oligoclonal bands. Other body fluids discussed include amniotic fluid, peritoneal fluid, and pleural fluid.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 14

    proteins in other body

    fluids
    30/5/2015

    Protein in other body fluids


    Urinary proteins:
    proteinuria
    Plasma Protein from blood & kidney
    extraneous sources : prostate , vigina
    Quantitative measurement performed
    from timed urine (24hr urine protein)
    The volume should accurately measured

    Protein in other body fluids

    CSF proteins
    15 to 45 mg/dl
    Increased CSF proteins:
    increased permeability of the capillary
    endothelial barrier
    bacterial, viral, and fungal meningitis; traumatic
    tap; multiple sclerosis; obstruction; neoplasm
    CSF protein is compared with serum albumin
    CSF albumin: plasma albumin is less than2.7:7.3
    Ratio more this indicates increase pearmeability

    CSF protein
    Decreased CSF proteins:
    Leak of CSF from a tear in the dura
    due to severe trauma
    Otorrohea: leak of CSF from ear
    Rhinorrohea: leak of CSF from nose

    CSF protein
    Increased CSF proteins:
    Lysis of contaminant blood from traumatic
    tap
    Increased permeability of epithelial
    membrane (blood-brain barrier)
    CNS bacterial or fungal infections
    Cerebral hemorrhages

    Increased production by CNS tissue as in


    cases of:
    Multiple sclerosis (MS)
    Subacute sclerosing panencephalitis (SSPE)

    Obstruction as in cases of :
    Tumors or abscess

    CSF protein
    Analysis of protein fractions: (Albumin & IgG)

    1- Albumin of CSF
    is obtained from blood by means of blood-brain barrier (as it is
    produced solely by the liver)
    In cases of increased permeability of BBB, albumin is increased in
    CSF

    2- IgG of CSF can be obtained:


    from blood (By BBB) : increase in cases of increase permeab. of BBB
    & by local synthesis from plasma cells within CSF (increased in cases of
    MS)

    How to determine the source of CSF IgG

    1. CHECK INTEGRITY OF BLOOD


    BRAIN BARRIER (BBB)

    BY CSF / serum albumin index


    calculation
    CSF serum albumin index = CSF
    albumin
    (mg/dl) / serum
    albumin (g/dl)
    Index less than 9 indicates intact
    BBB (no
    increased permeability of
    BBB)

    How to determine the source of CSF IgG


    2. CSF IgG INDEX IS CALCULATED
    CSF IgG / Serum IgG
    CSF IgG index =
    --------------------------------------CSF albumin /
    serumalbumin
    Normal : less than 0.7
    Increased in cases of demylineating diseases
    of CNS as : Multiple sclerosis (MS)

    CSF Immunoglobulin
    CSF IgG/Serum IgG
    =
    CSF serum /Albumin index

    CSF IgG index:


    Normally: < 0.7

    CSF IgG can arise:

    from plasma cells within CSF

    & from the blood through BBB


    CSF [IgG] without concomitant in CSF [Alb]
    suggests local production of IgG:

    multiple sclerosis (MS)

    subacute sclerosing panencephalitis (SPEE)

    CSF Electrophoresis: Oligoclonal Banding

    Electrophoresis patterns of CSF fro


    patients who have multiple
    sclerosis have multiple distinct
    oligoclonal bands in the zone

    Electrophoresis patterns of CSF fro


    patients who have multiple
    sclerosis have multiple distinct
    oligoclonal bands in the zone

    Protein in other body fluids


    Amniotic fluid : usually for AFP
    analysis
    Peritoneal & Pleural cavities:
    Transudates: total protein less than 3g/dl
    Exudates: total protein more than 3g/dl

    The ratio of pleural fluid protein to


    serum protein is greater than 0.5

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