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    6 Discussion (72 73)

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    mithun_nm
    The document discusses the isolation and characterization of compounds from Hemidesmus indicus that can serve as chemical markers for standardization. Four compounds (HI-01, HI-02, HI-03, HI-004) were isolated from the plant's roots via column chromatography. HI-01 and HI-004 were determined to be the purest compounds and were further characterized. HI-01 was identified as the pentacyclic triterpene 20(30)-Ursen-3-acetate and HI-004 as the aromatic aldehyde 2-Hydroxy-4-Methoxy benzaldehyde. HPLC analysis found these compounds present in 14.34% and 12

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    6 Discussion (72 73)

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    mithun_nm
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    The document discusses the isolation and characterization of compounds from Hemidesmus indicus that can serve as chemical markers for standardization. Four compounds (HI-01, HI-02, HI-03, HI-004) were isolated from the plant's roots via column chromatography. HI-01 and HI-004 were determined to be the purest compounds and were further characterized. HI-01 was identified as the pentacyclic triterpene 20(30)-Ursen-3-acetate and HI-004 as the aromatic aldehyde 2-Hydroxy-4-Methoxy benzaldehyde. HPLC analysis found these compounds present in 14.34% and 12

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    6 Discussion(72 73)

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    The document discusses the isolation and characterization of compounds from Hemidesmus indicus that can serve as chemical markers for standardization. Four compounds (HI-01, HI-02, HI-03, HI-004) were isolated from the plant's roots via column chromatography. HI-01 and HI-004 were determined to be the purest compounds and were further characterized. HI-01 was identified as the pentacyclic triterpene 20(30)-Ursen-3-acetate and HI-004 as the aromatic aldehyde 2-Hydroxy-4-Methoxy benzaldehyde. HPLC analysis found these compounds present in 14.34% and 12

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    75 views2 pages

    6 Discussion (72 73)

    Uploaded by

    mithun_nm
    The document discusses the isolation and characterization of compounds from Hemidesmus indicus that can serve as chemical markers for standardization. Four compounds (HI-01, HI-02, HI-03, HI-004) were isolated from the plant's roots via column chromatography. HI-01 and HI-004 were determined to be the purest compounds and were further characterized. HI-01 was identified as the pentacyclic triterpene 20(30)-Ursen-3-acetate and HI-004 as the aromatic aldehyde 2-Hydroxy-4-Methoxy benzaldehyde. HPLC analysis found these compounds present in 14.34% and 12

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    Download as DOC, PDF, TXT or read online from Scribd
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    of 2

    DISCUSSION

    DISCUSSION

    Hemidesmus indicus (L.) R.Br. is an important drug of Indian System of

    Medicine and used in medicines since time immemorial. A number of phytochemical

    studies have been carried out and many of the compounds reported are mainly
    19-27
    triterpenoids, glycosides, organic acids, aldehydes etc . Various pharmacological

    activities have been reported such as anticancer, hepatoprotective, anti snake-venom

    activity, anti-ulcerogenic, antiatherogenic, anti-inflammatory, analgesic, antileprotic and

    antimicrobial etc 28-64. Although many works have been carried out on this plant but there

    are scanty reports on specific markers for this plant, HPLC profile of the compounds and

    standardization. Hence in the present study an attempt had been made to isolate and

    standardize the constituents which are unique to this plant and characterize them as

    markers which are chemically defined constituents and can serve as a powerful tool for

    standardization.

    Isolation of the phytoconstituents

    The methanolic root extract of Hemidesmus indicus was refluxed with acetone.

    The acetone extract was then fractionated using silica gel column chromatography (60-

    200 mesh). Review of literature indicates that column chromatography using silica gel is

    the most popular method for isolation 19-27. Totally four different column chromatography

    has been done with different solvent systems which resulted in the isolation of four

    different compounds. These were given codes as HI-01, HI-02, HI-03 and HI-004.

    Department of Pharmacognosy - PESCP 72


    DISCUSSION

    Analysis of isolated compounds

    Determination of Melting point and solubility profile were done for the isolated

    compounds. New HPLC methods were developed to analyze the purity of the

    compounds. The HPLC method indicated that the compound HI-01 was 99.41 % pure,

    HI-02 was 32.88 % pure, HI-03 was 46.82% pure and HI-004 was 99.84 % pure. Hence

    depending upon the purity only HI-01 and HI-004 were taken for the further

    characterization.

    Characterization of the isolated compounds

    The integral values of NMR spectroscopy, the values where the bands obtained

    in the IR spectroscopy, the CHN analysis and the Mass spectroscopic analysis clearly

    suggested that the compound HI-01 was found to be a pentacyclic triterpene acetate. The

    chemical name suggested for this was 20(30)-Ursen-3-acetate. The other compound that

    is HI-004 was found to be an aromatic aldehyde and the chemical name suggested for this

    compound was 2-Hydroxy-4-Methoxy benzaldehyde 72, 73.

    Estimation of isolated compounds

    In order to check the suitability of the isolated compounds as markers, HPLC

    analytical methods were developed. The distinct peaks at the retention time of 1.694 min.

    and 26.021 min. (corresponding to the same value of HI-01 and HI-004 respectively) in

    HPLC chromatograms of acetone fraction was observed indicating that the method was

    suitable and sensitive. The acetone fraction of the MeOH extract was found to contain

    14.34 % of HI-01 and 12.94 % of HI-004. Hence these compounds can be termed as

    markers and the extract is said to be standardized.

    Department of Pharmacognosy - PESCP 73

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