Scribd Logo
Upload

Welcome to Scribd!

  • 1 views

    FSI27203 TOPIC 9 Plant and Tissue Culture

    Uploaded by

    m-4668898

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    FSI27203 TOPIC 9 Plant and Tissue Culture

    Uploaded by

    m-4668898
    1 views32 pages

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    1 views32 pages

    FSI27203 TOPIC 9 Plant and Tissue Culture

    Uploaded by

    m-4668898

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 32

    TOPIC 9: PLANT CELL AND

    TISSUE CULTURE
    Plant tissue culture is a collection of
    techniques used to maintain or grow plant
    cells, tissues or organs, under sterile
    conditions on a nutrient culture medium of
    known composition.

    Plant tissue culture is widely used to


    produce clones of a plant in a method
    known as micropropagation
    Living tissue transferred
    from a plant to an artificial
    medium for culture.

    It can be any portion of


    the shoot, leaves, roots,
    flower or cells from a plant.
    Adult plant cells are totipotent, meaning they
    have the ability to give rise to a fully
    differentiated plant. Because of this, it is
    possible to collect cells from a mature plant
    and use those cells to produce clones of that
    plant.
    https://www.youtube.com/watch?v=Hf6S
    siPn7_U&t=15s
    3 fundamental principals:
    • The plant parts must be isolated from the rest
    of the body
    • The plant parts (explant; isolated cells that is
    not affected) must be in a controlled, preferably
    defined, nutrient medium
    • Aseptic conditions must be maintained
    • Callus culture
    • Cell suspension culture
    • Anther culture
    • Ovule culture
    • Embryo culture
    • Protoplast culture
    • Micropropogation
    When an excised and isolated piece of tissue is
    cultured on a nutrient medium, an unorganized
    mass of cell appears, is called callus. This callus
    is transferred on to different media to
    regenerate plants. This technique is called tissue
    culture.
    It is the culture of isolated cells or very small cell
    aggregates dispersed in liquid medium. The cell
    suspension is obtained by agitating pieces of
    callus in liquid medium on gyrating shaker.
    Anther culture is a mean to produce plants with a
    gametic number of chromosomes by aseptic
    culture of anther. The technique give rise to
    haploid plants either directly or through
    formation of a haploid callus.
    Ovule culture techniques is an important
    technique in modern plant breeding. it is much
    easier to culture whole ovule than to isolate a
    single embryo, especially in small seeded plants.
    E.g., tobacco
    The embryos are isolated from young seeds and
    placed on a solid medium containing nutrients
    and vitamins. Embryos are cultured at 25°c,first
    in dark until seedlings are about 2 cm long and
    root formation has started, and than in light until
    the seedlings can be planted in soil.
    It is one of the most significant and recent
    developments in the field of plant tissue culture.
    the protoplast are usually isolated from cultured
    cell or leaf mesophyll cell by treating them with
    enzyme solutions. the isolated protoplast may be
    used to regenerate the plants directly, or for the
    production of somatic hybrids through fusion.
    It is in vitro asexual propagation of crop plants. This
    technique is advantages over the conventional
    practice of asexual propagation as only a small
    amount of plant is needed, species highly resistant
    to conventional bulk propagation can be
    propagated by this method and it is non season
    dependent. Micro propagation is used for rapid
    multiplication of stocks, elimination of diseases,
    germ plasm preservation, and induction of
    mutation.

    You might also like