3

Test Name: Indirect hemagglutination test for Entamoeba histolytica (Amoeba)

Cerner code ‐ Entamoeba Histolytica Antibody)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Twice a week
performed
Turnaround time 7 days

Method Quantitative detection of specific antibodies to Entamoeba

histolytica in human serum using indirect hemagglutination test
(IHA).

Reference Value Titer – range: 1:80 to 1:2560

Interpretation  Titer < 1:80 Negative (Considered Negative)

 1:80 ≤ Titer ≤1:160 Doubtful reaction.
 Titer ≥ 1:320 significant reaction in favour of visceral
amoebiasis.

Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.

 There is no doctor’s stamp.

 The request form contains no patient’s name and Hospital

or Qatar ID number.

 There is gross haemolysis in serum or plasma sample.

 An inadequate volume of specimen for testing is received.

 A blood specimen is lipemic

 The specimen has leaked

 The specimen is in a wrong containe

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference Kit insert ELITech MICROBIO– Amoeba

Classified as Confidential for Hamad Internal use,

Document: Lab Guide Amoebiasis 2023.docx Classification: Internal,
by [email protected], on 2023‐02‐09T07:36:08,
Test name: Quantitative tube agglutination test for serological diagnosis of Brucella abortus and Brucella melitensis
infections (Cerner code – Brucella Antibodies)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Twice a week
performed
Turnaround time 7 days

Method Quantitative tube agglutination test for Brucella antibodies

Reference Value Titre – range: 1:20 to 1:1280

Interpretation Titer 1:80 considered positive.

Titer (1:20 ‐ 1:80) is doubtful

Titer < 1:20 considered negative

Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.

 There is no doctor’s stamp.

 The request form contains no patient’s name and Hospital

or Qatar ID number.

 There is gross haemolysis in serum or plasma sample.

 An inadequate volume of specimen for testing is received.

 A blood specimen is lipaemic.

 The specimen has leaked

 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference Kit insert: Rapid Labs Febrile Antigens

Test Name: Chikungunya Virus IgG Antibody
(Cerner code – Chikungunya Virus IgG)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is As required
performed
Turnaround time 7 days

Method An enzyme linked immunosorbent assay (ELISA) for detection

of IgG antibodies to Chikungunya virus.

Reference Value Negative: Result by ratio < 0.8

Positive: Result by ratio ≥ 1.1

Equivocal: ≥ 0.8‐ <1.1

Interpretation  Chikungunya IgG positive & IgM negative: Past exposure to

Chikungunya virus or other Alphavirus (PCR test should be
done)
 Chikungunya IgG negative & IgM negative: No serological
evidence of exposure to Chikungunya Virus.
Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container for non HGH
facilities.
 The request form contains no patient’s name and Hospital
or Qatar ID number for non HGH facilities.
 There is gross haemolysis in serum or plasma sample.

 An inadequate volume of specimen for testing is received.

 A blood specimen is lipemic.

 The specimen has leaked.

 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference Anti‐ Chikungunya Virus IgG Elisa Euroimmune package insert.

Test name: Chikungunya Virus IgM Antibody
(Cerner code – Chikungunya Virus IgM)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is As required
performed
Turnaround time 7 days

Method An enzyme linked immunosorbent assay (ELISA) for detection

of IgM antibodies to Chikungunya virus.

Reference Value Negative: Result by ratio < 0.8

Positive: Result by ratio ≥ 1.1

Equivocal: ≥ 0.8‐ <1.1

Interpretation  Chikungunya IgM Ab positive: Chikungunya probable case

(PCR test should be done)
Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container for non HGH
facilities.
 The request form contains no patient’s name and Hospital
or Qatar ID number for non HGH facilities.
 There is gross haemolysis in serum or plasma sample.

 An inadequate volume of specimen for testing is received.

 A blood specimen is lipemic.

 The specimen has leaked.

 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference Anti‐Chikungunya Virus IgM Elisa Euroimmune package insert.

Test name: Cytomegalovirus IgG Avidity Antibody

(Cerner code ‐ Cytomegalovirus IgG Avidity) Department order only

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐ Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Twice a week
performed
Turnaround time 7 days

Method A Chemiluminescent Microparticle Immunoassay (CMIA) for the

determination of the avidity of IgG antibodies to
Cytomegalovirus in human serum and plasma.
Reference Value Low Avidity < 50.0 %Avi

Grayzone 50.0 – 59.9 %Avi

High Avidity ≥ 60.0 %Avi

Interpretation  If the CMV IgG Avidity results are inconsistent with clinical
evidence, additional testing is suggested to confirm the
result.
 For diagnostic purposes, results should be used in
conjunction with other data; e.g., results of other tests (CMV
IgG, CMV IgM), clinical impressions, etc.
Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and Hospital or
Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is received.
 A blood specimen is lipemic.
 The specimen has leaked
 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference Kit insert: Architect CMV IgG Avidity

Test name: Qualitative determination of lipid antibodies in CSF to VDRL antigen
(Cerner code – VDRL Screen CSF)
ITEM PROCESS
Specimen  Collect CSF, minimum 2ml, in plain sterile screw capped
container tube.
 Deliver promptly to Central Processing (CP) in QRI, 3rd
floor, HBKMC.
Transport Temperature Room temperature

Days test is performed Daily

Turnaround time 2 days
Method A macroscopic non‐treponemal flocculation test that is
used to detect lipid antibodies in CSF to modified VDRL
cardiolipin antigen
Reference Value  Negative: No flocculation
 Positive: Flocculation titer of ≥ 1:1
Interpretation Positive results in association with positive syphilis
specific tests indicate current untreated infection.
Rejection Criteria  Patient information on request form does not match
with information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and
Hospital or Qatar ID number.
 Blood stained CSF
 An inadequate volume of specimen for testing is
received.
 The specimen has leaked.
 The specimen is in a wrong container
Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine &
Location Pathology, located on 3rd floor of building #21, Hamad
Medical City, (ext#5124, 5125).
Reference BD VDRL Antigen package insert.
 Test name: Demgue IgG
(Cerner code ‐ Dengue Virus IgG)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Twice a week
performed
Turnaround time 7 days

Method Detection of IgG antibody by ELISA

Reference Value Negative: Result by ratio < 0.8

Positive: Result by ratio ≥ 1.1

Equivocal: ≥ 0.8‐ <1.1

Interpretation  ‐Positive IgG result indicates current or past infection (PCR

test should be done, if clinically indicated)
 ‐ Gray zone result should be repeated in a fresh sample 2‐4
weeks later. If the result is still gray zone the sample has to
be considered negative.
Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and Hospital or
Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is received.
 A blood specimen is lipemic.
 The specimen has leaked
 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference Kit insert: Anti‐Dengue Virus Elisa IgG, Euroimmune.

Classified as Confidential for Hamad Internal use,

Document: Lab Guide Dengue IgG 2023.docx Classification: Internal,
by [email protected], on 2023‐02‐09T10:21:07,
Test name: Demgue IgM
(Cerner code ‐ Dengue Virus IgM)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CPin QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Twice a week
performed
Turnaround time 7 days

Method Detection of IgM antibody by ELISA

Reference Value Negative: Result by ratio < 0.8

Positive: Result by ratio ≥ 1.1

Equivocal: ≥ 0.8‐ <1.1

Interpretation  ‐ Positive IgM result indicates current acute infection(PCR

test should be done)
 ‐ Gray zone result should be repeated in a fresh sample 2‐4
weeks later. If the result is still gray zone the sample has to
be considered negative.
Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and Hospital or
Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is received.
 A blood specimen is lipemic.
 The specimen has leaked
 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference Kit insert: Anti‐Dengue Virus Elisa IgM, Euroimmune

Test name: Indirect hemagglutination test for Echinoccocus granulosus
(Cerner code – Echinococcus Antibody)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in QRI 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Twice a week
performed
Turnaround time 7 days

Method Quantitative detection of specific antibodies to Echinococcus

granulosus in human serum using indirect hemagglutination
test (IHA).

Reference Value Titer – range: 1:80 to 1:2560

Interpretation  Titer < 1:80 Negative (Considered Negative)

 Titer < 1:160 Non significant reaction (Repeat after 2‐3
weeks)
 Titer = 1:160 Doubtful reaction (Repeat the test 2 to 3
weeks later)
 Titer ≥ 1:320 Significant reaction in favour of progressive
hydatidosis (Considered Positive)

Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.

 There is no doctor’s stamp.

 The request form contains no patient’s name and Hospital

or Qatar ID number.

 There is gross haemolysis in serum or plasma sample.

 An inadequate volume of specimen for testing is received.

 A blood specimen is lipemic

 The specimen has leaked.

 The specimen is in a wrong container

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).
Reference Kit insert ELITech MICROBIO– Echinococcosis
Test name: Hepatitis B Surface Antigen Confirmatory (HBsAg Conf)
(Cerner code – Hepatitis B Surface Antigen Conf) Test is only department order

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in Hamad General
Hospital
Transport Room temperature
Temperature
Days test is Daily
performed
Turnaround time 7 days

Method A Chemiluminescent Microparticle Immunoassay (CMIA) for

confirmation of the presence of Hepatitis B Surface Antigen
(HBsAg) using Architect.

Reference Value  Samples with HBsAgQ2 C2 <0.70 S/CO

Not‐confirmed

 Samples with HBsAgQ2 C2 <10 S/CO + Neutralization% <50%

Not‐confirmed
 Samples with HBsAgQ2 C2 ≥0.70 S/CO + Neutralization%
≥50% Confirmed Positive
Interpretation HBsAg Confirmatory is used to confirm gray zone, weak or low
positive Hepatitis B surface Ag.
Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and Hospital or
Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is received.
 A blood specimen is lipemic.
 The specimen has leaked
 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference Architect Hepatitis B Surface Antigen Qualitative II

Confirmatory package insert
Test Name: Hepatitis Delta Virus Total Antibody
(Cerner code – Hepatitis D Total Antibody)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Once a week
performed
Turnaround time 2 weeks

Method An enzyme linked immunosorbent assay for detection of total

antibodies to Hepatitis D virus.

Reference Value  Ratio between the cut‐off value and the sample < 0.9,
specimen considered Non‐Reactive for HDV‐Total Ab.
 Ratio between the cut‐off value and the sample between
0.9 and 1.1, specimen considered Equivocal for HDV‐Total
Ab.
 Ratio between the cut‐off value and the sample >1.1,
specimen considered Reactive for HDV‐Total Ab.

Interpretation  HDV total Ab positive: Indicate possible exposure to

Hepatitis D virus.
 HDV total Ab negative: No evidence of exposure to HDV.
 Any patient showing an equivocal result should be re‐
tested on a second sample taken 1‐2 weeks after the initial
sample

Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and Hospital or
Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is received.
 A blood specimen is lipemic.
 The specimen has leaked
 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference Kit insert: Total anti‐HDV Dia Pro leaflet.

Test Name: Hepatitis E Virus IgG Antibody
(Cerner code – Hepatitis E Virus IgG)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Once a week
performed
Turnaround time 2 weeks

Method An enzyme linked immunosorbent assay for detection of IgG

antibodies to Hepatitis E virus.

Reference Value Negative: Result by ratio < 0.8

Positive: Result by ratio ≥ 1.1

Equivocal: ≥ 0.8‐ <1.1

Interpretation  HEV IgG positive: In absence of HEV IgM, indicates past

exposure to HEV.
 HEV IgG negative: No evidence of exposure to HEV.
Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and Hospital or
Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is received.
 A blood specimen is lipemic.
 The specimen has leaked
 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference HEV IgG Euroimmune package insert

Test name: Hepatitis E Virus IgM Antibody
(Cerner code – Hepatitis E Virus IgM)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Once a week
performed
Turnaround time 2 weeks

Method An enzyme linked immunosorbent assay for detection of IgM

antibodies to Hepatitis E virus.

Reference Value Negative: Result by ratio < 0.8

Positive: Result by ratio ≥ 1.1

Equivocal: ≥ 0.8‐ <1.1

Interpretation Anti‐HEV IgM positive: current or recent infection with the

virus.
Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and Hospital or
Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is received.
 A blood specimen is lipemic.
 The specimen has leaked
 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference HEV IgM Euroimmune package insert

Test name: HIV‐1 and HIV‐2 Antibody Confirmatory Assay
(Cerner code – HIV Blot Panel) Test is only department order

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Twice a week
performed
Turnaround time 7 days

Method The Geenius™ HIV 1/2 Supplemental Assay is a single‐use

immunochromatographic assay to confirm the presence & and
differentiation of antibodies against HIV‐1 and HIV‐2.

Reference Value  A sample is NEGATIVE if there no bands (lines) on the strip.

 A sample is POSITIVE if it meets kit manufacturer’s criteria for
positivity.
 A sample is INDETERMINATE if it does not meet criteria for
positivity or negativity
Interpretation  Although a sample which is confirmed positive for HIV‐1 or HIV‐2
antibodies indicates infection, clinical findings, exposure history
and other laboratory data must be taken into consideration before
a final diagnosis is reached.
 If an indeterminate result is obtained, it is recommended to test an
additional patient sample after a few weeks.
 A negative result does not preclude the possibility of exposure to
HIV or infection with the virus.
Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and Hospital or
Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is received.
 A blood specimen is lipemic.
 The specimen has leaked
 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference BioRad Geenius HIVI/II Supplemental Assay kit insert.

Test name: HTLV I/II Antibody Confirmatory Assay
(Cerner code – HTLV Blot Panel) Test is only department order

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Twice a week
performed
Turnaround time 7 days

Method A Line Immuno Assay (LIA) to confirm the presence of

antibodies against HTLV‐1 and HTLV‐2

Reference Value  A sample is NEGATIVE if:

1. If all HTLV antigen lines have a negative reactivity rating
2. If one HTLV antigen band (p19 I/II or p24 I/II or gp46 I/II) has a
reactivity of ≥±.
 A sample is POSITIVE if :
1. If two bands has a reactivity of ≥± and gp21 reactive.
2. If three or more bands reactive.
 A sample is INDETERMINATE if:
1. If single HTLV antigen line gp21 has a reactivity of ≥±.
2. If two bands has a reactivity of ≥± and gp21 NON reactive
Interpretation  Although a sample which is confirmed positive for HTLV‐1 or HTLV‐
2 antibodies indicates infection, clinical findings, exposure history
and other laboratory data must be taken into consideration before
a final diagnosis is reached.
 If an indeterminate result is obtained, it is recommended to test an
additional patient sample after a few weeks.
 A negative result does not preclude the possibility of exposure to
HTLV or infection with the virus.

Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and Hospital or
Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is received.
 A blood specimen is lipemic.
 The specimen has leaked
 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference FUJIREBIO INNO‐LIA HTLVI/II Score kit insert.

 Test name: Human T‐cell Lymphotropic Virus types I & II (HTLV‐I & HTLV‐II) Antibodies
(Cerner code – Human T Lymphotropic Virus Antibody)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Daily
performed
Turnaround time 7 days

Method A Chemiluminescent Microparticle Immunoassay (CMIA) for the

qualitative detection of antibodies to Human T‐cell
Lymphotropic Virus types I and II

Reference Value  Nonreactive (i.e. negative): S/CO <1.00

 Reactive (i.e positive): S/CO  1.00
Interpretation Presence of antibodies indicates exposure to HTLV‐I or
HTLV‐II. However, reactive samples need to be confirmed
with a Western blot.
Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and Hospital or
Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is received.
 A blood specimen is lipemic.
 The specimen has leaked
 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference Abbott, Architect package insert Human T Lymphotropic Virus

I/II Antibody.

Classified as Confidential for Hamad Internal use,

Document: Lab Guide HTLV‐I and HTLV‐II Antibodies.docx Classification: Internal,
by [email protected], on 2023‐02‐09T09:51:15,
Test Name: Leishmania donovani antibodies

(Cerner code – Leishmania Antibody)

ITEM PROCESS
Specimen  Collect blood, minimum 5ml, in yellow capped tube with
clot activator or in plain yellow or red capped tube.
 Deliver promptly to Central Processing (CP) ) in QRI, 3rd
floor, HBKMC.
Transport Temperature Room temperature

Days test is performed Twice a week

Turnaround time 7 days
Method Qualitative detection of Leishmania Ab in human serum or
plasma.
Reference Value  Positive (prensence of control band C & test band T)
 Negative (prensence of control bamd C only)
Interpretation  Positive sample should be confirmed by a supplemental
assay (ELISA)
Rejection Criteria  Patient information on request form does not match
with information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and
Hospital or Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is
received.
 A blood specimen is lipemic
 The specimen has leaked
 The specimen is in a wrong container
Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference Kit insert: OnSite Leishmania Ab, M/s. CTK Biotech, Inc
 Test name: Rapid Plasma Reagin card test for serodiagnosis of Syphilis
( Cerner code: RPR Test ) Test is only department order

ITEM PROCESS
Specimen  Collect blood, minimum 5ml, in yellow capped tube with
clot activator or in plain yellow or red capped tube.
 Deliver promptly to Central Processing (CP in QRI, 3rd
floor, HBKMC.
Transport Temperature Room temperature

Days test is performed Daily

Turnaround time 7 days
Method Non‐ treponemal agglutination test for the qualitative and
semi‐quantitative determination of regain antibodies in
human serum or plasma.
Reference Value Negative: No agglutination

Positive: Agglutination titer ≥ 1:1

Interpretation  RPR test is performed to monitor treatment.
 In conjunction with confirmatory assay, RPR is used to
determine current or past infection.
 This is a non‐treponemal test and is associated with
false positive reactions when the patient has infections
other than syphilis. Also it can be positive due to
pregnancy, drug addiction, collagen vascular disease
and Gaucher’s disease.
 If positive RPR result is found a specific treponemal
confirmatory test will be performed.
Rejection Criteria  Patient information on request form does not match
with information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and
Hospital or Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is
received.
 A blood specimen is lipemic
 The specimen has leaked
 The specimen is in a wrong container
Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine &
Location Pathology, located on 3rd floor of building #21, Hamad
Medical City, (ext#5124, 5125).
Classified as Confidential for Hamad Internal use,
Document: Lab Guide RPR.docx Classification: Internal,
by [email protected], on 2023‐02‐09T10:02:34,
 Reference BD Macro‐Vue RPR Card Tests

Classified as Confidential for Hamad Internal use,

Document: Lab Guide RPR.docx Classification: Internal,
by [email protected], on 2023‐02‐09T10:02:34,
Test name: Rubella IgG Avidity Antibody

(Cerner code ‐ Rubella IgG Avidity) Department order only

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐ Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Twice a week
performed
Turnaround time 7 days

Method ELISA technique for the determination of the avidity of IgG

antibodies to Rubella virus in human serum and plasma.
Reference Value RAI < 40% Indication of Low avidity antibodies

RAI < 40% ‐ 60% Equivocal range

RAI > 60% Indication of High avidity antibodies

Interpretation For diagnostic purposes, results should be used in conjunction

with other data; e.g., results of other tests (Rubella IgG and
IgM), clinical impressions, etc.

Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and Hospital
or Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is received.
 A blood specimen is lipemic.
 The specimen has leaked
 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine &

Location Pathology, located on 3rd floor of building #21, Hamad Medical
City, (ext#5124, 5125).

Reference Kit insert: Rubella IgG Avidity Euroimmune

Test Name: Indirect hemagglutination test for Schistosoma mansoni, Schistosoma
haematobium, and Schistosoma intercalatum

(Cerner code – Schistosoma Antibody)

ITEM PROCESS
Specimen  Collect blood, minimum 5ml, in yellow capped tube
with clot activator or in plain yellow or red capped
tube.
 Deliver promptly to Central Processing (CP) in QRI, 3rd
floor, HBKMC.
Transport Temperature Room temperature

Days test is performed Twice a week

Turnaround time 7 days
Method Quantitative detection of specific antibodies to
Schistosoma mansoni, Schistosoma haematobium, and
Schistosoma intercalatum in human serum using indirect
hemagglutination test (IHA).
Reference Value Titre – range: 1:80 to 1:2560
Interpretation  Titre < 1:80 Negative
 Titer < 1:160 Non significant reaction (Repeat after 2‐3
weeks)
 Titer ≥1:160 significant reaction Presumption of an
active infection. Considered positive
Rejection Criteria  Patient information on request form does not match
with information on the sample tube/container.
 There is no doctor’s stamp.
 The request form contains no patient’s name and
Hospital or Qatar ID number.
 There is gross haemolysis in serum or plasma sample.
 An inadequate volume of specimen for testing is
received.
 A blood specimen is lipemic
 The specimen has leaked
 The specimen is in a wrong container
Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine &
Location Pathology, located on 3rd floor of building #21, Hamad
Medical City, (ext#5124, 5125).
Reference Kit insert: ELITech MICROBIO– Schistosoma
Test name: TPHA
(Cerner code – Treponema pallidum hemagglutination)

ITEM PROCESS
Specimen 1-Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2-Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Twice a week
performed
Turnaround time 7 days

Method Haemagglutination method

Reference Value No reference

Interpretation Negative
Positive titer 1:80, 1:160, 1:320, 1:640, ... upto 1:10240
Rejection Criteria • Patient information on request form does not match with
information on the sample tube/container for non HGH
facilities.
• The request form contains no patient’s name and Hospital
or Qatar ID number for non HGH facilities.
• There is gross haemolysis in serum or plasma sample.

• An inadequate volume of specimen for testing is received.

• A blood specimen is lipemic.

• The specimen has leaked.

• The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine &
Location Pathology, located on 3rd floor of building #21, Hamad Medical
City, (ext#5124, 5125).

Reference BioRad TPHA kit insert

Test name: Toxoplasma IgG Avidity Antibody

(Cerner code - Toxoplasma IgG Avidity) Department order only

ITEM PROCESS
Specimen 1-Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2- Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is Twice a week
performed
Turnaround time 7 days

Method A Chemiluminescent Microparticle Immunoassay (CMIA) for the

determination of the avidity of IgG antibodies to Toxoplasma
gondii in human serum and plasma.
Reference Value Low Avidity < 50.0 %Avi

Grayzone 50.0 – 59.9 %Avi

High Avidity ≥ 60.0 %Avi

Interpretation • If the Toxo IgG Avidity results are inconsistent with clinical
evidence, additional testing is suggested to confirm the
result.
• For diagnostic purposes, results should be used in
conjunction with other data; e.g., results of other tests
(Toxo IgG, Toxo IgM), clinical impressions, etc.
Rejection Criteria • Patient information on request form does not match with
information on the sample tube/container.
• There is no doctor’s stamp.
• The request form contains no patient’s name and Hospital or
Qatar ID number.
• There is gross haemolysis in serum or plasma sample.
• An inadequate volume of specimen for testing is received.
• A blood specimen is lipemic.
• The specimen has leaked
• The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine & Pathology,
Location located on 3rd floor of building #21, Hamad Medical City, (ext#5124,
5125).

Reference Kit insert: Abbott, Architect Toxo IgG Avidity

Test Name: Zika Virus IgG Antibody
(Cerner code – Zika Virus IgG)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is As required
performed
Turnaround time 7 days

Method An enzyme linked immunosorbent assay (ELISA) for detection

of IgG antibodies to Zika virus.

Reference Value Negative: Result by ratio < 0.8

Positive: Result by ratio ≥ 1.1

Equivocal: ≥ 0.8‐ <1.1

Interpretation  Zika IgG positive & IgM negative: Past exposure to Zika virus
or other flavivirus (PCR test should be done)
 Zika IgG negative & IgM negative: No serological evidence
of exposure to Zika Virus.
Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container for non HGH
facilities.
 The request form contains no patient’s name and Hospital
or Qatar ID number for non HGH facilities.
 There is gross haemolysis in serum or plasma sample.

 An inadequate volume of specimen for testing is received.

 A blood specimen is lipemic.

 The specimen has leaked.

 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine &
Location Pathology, located on 3rd floor of building #21, Hamad Medical
City, (ext#5124, 5125).

Reference Anti‐Zika Virus IgG Elisa Euroimmune package insert.

Test name: Zika Virus IgM Antibody
(Cerner code – Zika Virus IgM)

ITEM PROCESS
Specimen 1‐Collect blood, minimum 5ml, in yellow capped tube with clot
activator or in plain yellow or red capped tube.
2‐Deliver promptly to Central Processing (CP) in QRI, 3rd floor,
HBKMC.
Transport Room temperature
Temperature
Days test is As required
performed
Turnaround time 7 days

Method An enzyme linked immunosorbent assay (ELISA) for detection

of IgM antibodies to Zika virus.

Reference Value Negative: Result by ratio < 0.8

Positive: Result by ratio ≥ 1.1

Equivocal: ≥ 0.8‐ <1.1

Interpretation  Zika IgM Ab positive: Zika probable case (PCR test should
be done)
Rejection Criteria  Patient information on request form does not match with
information on the sample tube/container for non HGH
facilities.
 The request form contains no patient’s name and Hospital
or Qatar ID number for non HGH facilities.
 There is gross haemolysis in serum or plasma sample.

 An inadequate volume of specimen for testing is received.

 A blood specimen is lipemic.

 The specimen has leaked.

 The specimen is in a wrong container.

Performing Lab Virology Serology Laboratory, Dept. of Lab Medicine &
Location Pathology, located on 3rd floor of building #21, Hamad Medical
City, (ext#5124, 5125).

Reference Anti‐Zika Virus IgM Elisa Euroimmune package insert.