Scribd Logo
Upload

Welcome to Scribd!

  • 106 views

    Nissl

    Uploaded by

    Janak Awasthi
    This document provides instructions for staining nerve tissue samples with Cresyl Echt Violet to demonstrate Nissl substance. Nissl substance is composed primarily of rough endoplasmic reticulum and is basophilic, allowing it to be sharply stained. The procedure involves deparaffinizing and hydrating tissue sections, staining with 0.5% Cresyl Echt Violet for two minutes, washing, dehydrating, clearing in xylene, and coverslipping. Nissl substance will be dark blue to purple under results. Normal spinal cord is used as a control sample.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Nissl

    Uploaded by

    Janak Awasthi
    106 views4 pages
    This document provides instructions for staining nerve tissue samples with Cresyl Echt Violet to demonstrate Nissl substance. Nissl substance is composed primarily of rough endoplasmic reticulum and is basophilic, allowing it to be sharply stained. The procedure involves deparaffinizing and hydrating tissue sections, staining with 0.5% Cresyl Echt Violet for two minutes, washing, dehydrating, clearing in xylene, and coverslipping. Nissl substance will be dark blue to purple under results. Normal spinal cord is used as a control sample.

    Original Description:

    protocol for nissl stain

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document provides instructions for staining nerve tissue samples with Cresyl Echt Violet to demonstrate Nissl substance. Nissl substance is composed primarily of rough endoplasmic reticulum and is basophilic, allowing it to be sharply stained. The procedure involves deparaffinizing and hydrating tissue sections, staining with 0.5% Cresyl Echt Violet for two minutes, washing, dehydrating, clearing in xylene, and coverslipping. Nissl substance will be dark blue to purple under results. Normal spinal cord is used as a control sample.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    106 views4 pages

    Nissl

    Uploaded by

    Janak Awasthi
    This document provides instructions for staining nerve tissue samples with Cresyl Echt Violet to demonstrate Nissl substance. Nissl substance is composed primarily of rough endoplasmic reticulum and is basophilic, allowing it to be sharply stained. The procedure involves deparaffinizing and hydrating tissue sections, staining with 0.5% Cresyl Echt Violet for two minutes, washing, dehydrating, clearing in xylene, and coverslipping. Nissl substance will be dark blue to purple under results. Normal spinal cord is used as a control sample.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    SURGICAL PATHOLOGY - HISTOLOGY Date:

    STAINING MANUAL - NERVE TISSUE Page: 1 of 2


    NISSL SUBSTANCE - CRESYL ECHT VIOLET STAIN
    PURPOSE: To demonstrate Nissl substance in tissue sections. Nissl
    substance is lost after cell injury and if the axon degenerates, the myelin
    covering also breaks down.

    PRINCIPLE: Neurons contain Nissl substance, which is primarily


    composed of rough endoplasmic reticulum, with the amount, form, and
    distribution varying in different types of neurons. Because of the RNA
    content, Nissl substance is very basophilic and will be very sharply
    stained with basic aniline dyes. By varying the pH and the degree of
    differentiation, both Nissl substance and nuclei or only Nissl substance
    may be demonstrated.

    CONTROL: Normal spinal cord.

    FIXATIVE: 10% formalin

    TECHNIQUE: Cut paraffin sections at 6 microns. Allow to dry overnight at


    37°C.

    EQUIPMENT: Coplin jars, rinse all glassware in DI water.

    REAGENTS:
    0.5% Cresyl Echt Violet:
    Cresyl Echt Violet Acetate 0.5 gm
    Distilled water 100.0 ml

    Mix well, filter. For best results


    allow to ripen 48 hours. Stable for
    1 year.

    SAFETY: Wear gloves, goggles and lab coar. Avoid contact and inhalation.

    PROCEDURE:
    1. Deparaffinize and hydrate to distilled water.
    2. Cresyl violet, two minutes.
    3. Wash in distilled water.
    4. Dehydrate, clear in xylene, coverslip.
    NERVE TISSUE
    CRESYL ECHT VIOLET Page: 2 of 2
    RESULTS:
    Nissl substance dark blue to purple

    NOTE: If differentiation is needed use 70% alcohol.

    REFERENCES:
    Carson F, Histotechnology: A Self-Instructional Text, 1990, pp 170-171,
    ASCP, Ill

    Prepared: By:

    Approved: By:
    Downloaded from WebPath: Internet Pathology Laboratory
    http://www-medlib.med.utah.edu/WebPath/webpath.html
    PROCEDURE CARD
    NISSL SUBSTANCE - CRESYL ECHT VIOLET STAIN
    CONTROL: Normal spinal cord.

    TECHNIQUE: Cut paraffin sections at 6 microns. Allow to dry overnight at 37°C.

    PROCEDURE:
    1. Deparaffinize and hydrate to distilled water.
    2. Cresyl violet, two minutes.
    3. Wash in distilled water.
    4. Dehydrate, clear in xylene, coverslip.

    RESULTS:
    Nissl substance dark blue to purple

    NOTE: If differentiation is needed use 70% alcohol.

    0.5% Cresyl Echt Violet:


    Cresyl Echt Violet Acetate 0.5 gm
    Distilled water 100.0 ml
    Mix well, filter. For best results allow to
    ripen 48 hours. Stable for 1 year.
    0.5% CRESYL ECHT VIOLET:
    Cresyl Echt Violet Acetate 1.0 gm
    Distilled water 200.0 ml

    Mix well, filter. For best results


    allow to ripen 48 hours. Stable.

    DATE:

    TECH:

    EXPIRATION:

    You might also like