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    Molecular Biology and Genetics Techniques Report

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    Molecular Biology and Genetics Techniques Report

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    molecular biology and genetics techniques report

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    3 views1 page

    Molecular Biology and Genetics Techniques Report

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    althubitilayan1

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    © All Rights Reserved
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    of 1

    College of Medicine

    Fluorescence In Situ Hybridization (FISH)


    16/10/2024

    Students name:Layan Althubiti

    Students ID: 202322025

    Introduction:

    The technique known as Fluorescence In Situ Hybridization (FISH) uses fluorescently labeled probes to identify particular DNA or RNA sequences
    inside of cells. Under a fluorescence microscope this technique makes chromosomal abnormalities and gene expression patterns visible.

    Principle:

    In order for FISH to work a fluorescently labeled probe must hybridize with the complementary DNA or RNA sequence found in a sample of cells or
    tissue. The fluorescent markers signal enables the target sequence to be located.

    Procedure:

    1. Sample Prep: Fix cells/tissue on a slide.

    2. Probe Labeling: Create a fluorescently labeled DNA/RNA probe.

    3. Denaturation: Denature the DNA sample and the probe.

    4. Hybridization: Apply the probe to the sample.

    5. Detection: Use a fluorescence microscope to visualize bound probes.

    Applications:

    1. Cancer Diagnosis: Detect chromosomal abnormalities linked to cancer.


    2. Genetic Testing: Identify genetic disorders in prenatal or postnatal samples.
    3. Gene Mapping: Locate specific genes on chromosomes.
    4. Cytogenetics: Study chromosomal structure and abnormalities.

    References:

    1. Haugland, R. P. (2005). Handbook of Fluorescent Probes.


    2. Lakowicz, J. R. (2006). Principles of Fluorescence Spectroscopy.
    3. Sambrook, J., & Russell, D. W. (2001). Molecular Cloning.
    4. Tyagi, S., & Kramer, F. R. (1996). Nature Biotechnology, 14(3), 303-308.
    5. Hermanson, G. T. (2013). Bioconjugate Techniques.

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