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    Biology Practical 7

    Uploaded by

    Md Nawazish
    Biology

    Copyright:

    © All Rights Reserved
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    Biology Practical 7

    Uploaded by

    Md Nawazish
    3 views8 pages
    Biology

    Original Title

    biology practical 7

    Copyright

    © © All Rights Reserved

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    PDF, TXT or read online from Scribd

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    Biology

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    3 views8 pages

    Biology Practical 7

    Uploaded by

    Md Nawazish
    Biology

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 8

    ~

    To study the distribution of stomata on upper and lower


    surfaces of leaf and to calculate the stomatal index

    Fresh leaf of Petunia ( a dlcot plant) and of LIiy ( a monocot


    ;;!
    ~
    plant) ,forecep1,neeclle,bru1h,watch glan,petrtdlsh,glau
    1lld11,coversllp, safranine,glycertne,water
    0

    ~ I ;(_ ]~ ~ ~ ~
    -

    ..
    Stomata are tiny microscopic structures
    ' 'I. .
    -
    . .

    prasant in leaves of all flowering plants.Number


    and distribution of stomata per unit area is
    1• II ,
    variable In leaves of different plants.
    The leaves of most dicot plants poues stomata on~
    on lower surface.However leaves of some mesophytic '

    ..........
    plants ( potato,bean,petunia) pos111 stomata on the
    upper surface also. In monocots stomata are iai r~
    "-~-· u.:'
    distributed equal~ on two surfaces of leaves.
    Atypical stomata consists of apair of guard cells and
    an aperture in the centre called stomatal aperture.
    ~'" .
    l■rt>cedure
    • Take out epidermal peel from upper
    and lower surfaces of Petunia leaf
    and put them in separate watch glasses
    •Stain the peels with safranin by adding few drops of the stain in the watch gluHS
    -I
    •Take the peels and mount them in glycerine on sen~9'!1te slides cq'
    •
    . Cover the peels with coverslips and •
    observe under the microscope
    •Count the numbers of stomata and the numbers of Petunia
    epidermal cells in the peels of both upper and lower
    epidermis of the leaf appearing in the microscopic field
    Upper epidu1111s lowtr rp1der"1is

    • Similarly prepare the slides of the peels of lily and count the numbers of
    stomata and numbers of epidermal cells in the microscopic field
    Upptr tptdtrl'MII Lowrr epiden1"111

    Lij_y
    fe>rmula used ~(). Of St()m ata (S)
    Stomatal Index =- - - - - - X 100
    No.Of stomata +No. of Epidermal cells
    (' Obs erva ti_
    ()_
    DS_ - 1 (S+ E)

    Petunia , 13 2 5 13
    LI~ 8 5 5 38
    1
    l<e sult s
    leaf of Petun ia .
    27
    St()matal inde, in l()wer surface ()f leaf =
    St()matal index in upper surface ()f leaf= 13
    It show·s that in Petunia the number of stomata is more in the lower
    epidermis and only few stomata are present in the upper epiderm is.
    Lea f of Lily
    Sttlmatal index in ltlwer and upper epidemis tlf leaf= 38
    It shows that the leaf of Lily has almost equal
    number of stomata in both the epidermis
    Calculate the stomatal index of
    the given upper and lower
    surfaces of leaf of a plant
    leleot where you oan find It
    Dicot
    Or Monoco t
    ecautit)ns
    1. The curlinll of leaves should be avoided
    2. ~lways use brush to transfer the peel
    fro01 watch ~lasses t() the slide.
    1. lxcess of ,1ycerine should be removed
    by usin~ blottin~ paper

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