U937-DC-SIGN

Product Sheet

CRL-3253™

Description
U937-DC-SIGN was developed by transducing lymphocyte U-937 cells with the gene
for human DC SIGN protein (Dendritic cell-specific intercellular adhesion molecule 3-
grabbing non-integrin), a known attachment factor for Dengue viruses. The cloning
vector used was pcDNA3 which contains SV-40 and CMV promoter regions. The U cell
line that was established in 2008 from the stable transfection of U-937 cells (ATCC
No. CRL-1593.2 isolated from a pleural effusion of a 37-year-old, white male patient
with histiocytic lymphoma. This cell line was deposited by A. Desilva.
Organism: Homo sapiens, human
Cell Type: lymphocyte
Tissue: Pleural effusion
Age: 37 years
Gender: Male
Morphology: lymphocyte-like
Growth properties: Suspension
Disease: Histiocytic Lymphoma

Storage Conditions
Product format: Frozen
Storage conditions: Vapor phase of liquid nitrogen

Intended Use
This product is intended for laboratory research use only. It is not intended for any
animal or human therapeutic use, any human or animal consumption, or any
diagnostic use.

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BSL 2
ATCC determines the biosafety level of a material based on our risk assessment as
guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories
(BMBL), U.S. Department of Health and Human Services. It is your responsibility to
understand the hazards associated with the material per your organization’s policies
and procedures as well as any other applicable regulations as enforced by your local
or national agencies.

Cells contain cytomegalovirus (CMV) DNA sequences

Cells contain SV40 sequences

ATCC highly recommends that appropriate personal protective equipment is always

used when handling vials. For cultures that require storage in liquid nitrogen, it is
important to note that some vials may leak when submersed in liquid nitrogen and
will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid
nitrogen back to its gas phase may result in the vial exploding or blowing off its cap
with dangerous force creating flying debris. Unless necessary, ATCC recommends
that these cultures be stored in the vapor phase of liquid nitrogen rather than
submersed in liquid nitrogen.

Certificate of Analysis
For batch-specific test results, refer to the applicable certificate of analysis that can
be found at www.atcc.org.

Growth Conditions
Temperature: 37°C

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Atmosphere: 95% Air, 5% CO2

Handling Procedures
Unpacking and storage instructions:
1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately place the
cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until
ready for use.
Complete medium: The base medium for this cell line is ATCC-formulated RPMI-1640
Medium, Catalog No. 30-2001. To make the complete growth medium, add the
following components to the base medium:
○ an additional 2 mM L-glutamine
○ non-essential amino acids to a final concentration of 0.1 mM
○ 2-mercaptoethanol to a final concentration of 0.05 mM
○ fetal bovine serum to a final concentration of 5%
○ Subculturing procedure: Cultures can be maintained by the
addition of fresh medium or replacement of medium. Alternatively,
cultures can be established by centrifugation with subsequent
resuspension at 1 x 105 to 2 x 105 viable cells/mL.

Interval: Maintain cell density between 1 X 105 and 2 X 106 viable

cells/mL.

Medium Renewal: Add fresh medium every 3 to 4 days (depending

on cell density).

Material Citation
If use of this material results in a scientific publication, please cite the material in the
following manner: U937-DC-SIGN (ATCC CRL-3253)

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References
References and other information relating to this material are available at
www.atcc.org.

Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30

days from the date of shipment, provided that the customer has stored and handled
the product according to the information included on the product information sheet,
website, and Certificate of Analysis. For living cultures, ATCC lists the media
formulation and reagents that have been found to be effective for the product.
While other unspecified media and reagents may also produce satisfactory results, a
change in the ATCC and/or depositor-recommended protocols may affect the
recovery, growth, and/or function of the product. If an alternative medium
formulation or reagent is used, the ATCC warranty for viability is no longer valid.
Except as expressly set forth herein, no other warranties of any kind are provided,
express or implied, including, but not limited to, any implied warranties of
merchantability, fitness for a particular purpose, manufacture according to cGMP
standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any
animal or human therapeutic use, any human or animal consumption, or any
diagnostic use. Any proposed commercial use is prohibited without a license from
ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information
on this product sheet, ATCC makes no warranties or representations as to its
accuracy. Citations from scientific literature and patents are provided for
informational purposes only. ATCC does not warrant that such information has been
confirmed to be accurate or complete and the customer bears the sole responsibility

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of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and
assumes all risk and responsibility in connection with the receipt, handling, storage,
disposal, and use of the ATCC product including without limitation taking all
appropriate safety and handling precautions to minimize health or environmental
risk. As a condition of receiving the material, the customer agrees that any activity
undertaken with the ATCC product and any progeny or modifications will be
conducted in compliance with all applicable laws, regulations, and guidelines. This
product is provided 'AS IS' with no representations or warranties whatsoever except
as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries,
directors, officers, agents, employees, assigns, successors, and affiliates be liable for
indirect, special, incidental, or consequential damages of any kind in connection with
or arising out of the customer's use of the product. While reasonable effort is made
to ensure authenticity and reliability of materials on deposit, ATCC is not liable for
damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the
use of this product. The MTA is available at www.atcc.org.

Copyright and Trademark Information

© ATCC 2023. All rights reserved.
ATCC is a registered trademark of the American Type Culture Collection.

Revision
This information on this document was last updated on 2023-01-28

Contact Information
ATCC
10801 University Boulevard

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Manassas, VA 20110-2209
USA
US telephone: 800-638-6597
Worldwide telephone: +1-703-365-2700
Email: [email protected] or contact your local distributor

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