Product Sheet

Product Sheet
Lactobacillus vaginalis Embley et al.

49540™
Description
Type strain
• Strain designation NCTC 12197
• Deposited As Lactobacillus vaginalis Embley et al.
• Type strain Yes
Storage Conditions
• Product format Freeze-dried

• Storage conditions 2°C to 8°C
Intended Use
This product is intended for laboratory research use only. It is not intended for any animal or human
therapeutic use, any human or animal consumption, or any diagnostic use.
BSL 1
ATCC determines the biosafety level of a material based on our risk assessment as guided by the
current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S.
Department of Health and Human Services. It is your responsibility to understand the hazards
associated with the material per your organization’s policies and procedures as well as any other
applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when
handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some
vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon
thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding
or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC
recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed
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Product Sheet
49540
in liquid nitrogen.
Certificate of Analysis
For batch-specific test results, refer to the applicable certificate of analysis that can be found at
www.atcc.org.
Growth Conditions
• Medium
ATCC Medium 416: Lactobacilli MRS Agar/Broth
• Temperature 37°C
• Atmosphere 95% Air, 5% CO2
Handling Procedures
• 1. Open vial according to enclosed instructions.

2. Using a single tube of #416 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a
Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #416 agar slant and/or plate.
5. Incubate the tubes and plate at 37°C for 24 hours in an atmosphere of 5% CO2. Loosen the
screw caps during the incubation period.
Notes
After 24 hours, growth is evident by turbidity in the broth and the formation of small colonies on the
slant and/or plate. Growth is best in broth culture or on biphasic slants.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
www.atcc.org
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Product Sheet
49540
Material Citation
If use of this material results in a scientific publication, please cite the material in the following
manner: Lactobacillus vaginalis Embley et al. (ATCC 49540)
References
References and other information relating to this material are available at www.atcc.org.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from
the date of shipment, provided that the customer has stored and handled the product according to the
information included on the product information sheet, website, and Certificate of Analysis. For
living cultures, ATCC lists the media formulation and reagents that have been found to be effective
for the product. While other unspecified media and reagents may also produce satisfactory results, a
change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth,
and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC
warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of
any kind are provided, express or implied, including, but not limited to, any implied warranties of
merchantability, fitness for a particular purpose, manufacture according to cGMP standards,
typicality, safety, accuracy, and/or noninfringement.
Disclaimers
• This product is intended for laboratory research use only. It is not intended for any animal or human
therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed
commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product
sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific
literature and patents are provided for informational purposes only. ATCC does not warrant that
such information has been confirmed to be accurate or complete and the customer bears the sole
responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and
responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC
product including without limitation taking all appropriate safety and handling precautions to
minimize health or environmental risk. As a condition of receiving the material, the customer agrees
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Product Sheet
49540
that any activity undertaken with the ATCC product and any progeny or modifications will be
conducted in compliance with all applicable laws, regulations, and guidelines. This product is
provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth
herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees,
assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages
of any kind in connection with or arising out of the customer's use of the product. While reasonable
effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for
damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this
product. The MTA is available at www.atcc.org.
Copyright and Trademark Information
© ATCC 2023. All rights reserved.

ATCC is a registered trademark of the American Type Culture Collection.
Revision
This information on this document was last updated on 2022-08-13
Contact Information
ATCC
10801 University Boulevard
Manassas, VA 20110-2209
USA
US telephone: 800-638-6597
Worldwide telephone: +1-703-365-2700
Email: [email protected] or contact your local distributor
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Lactobacillus vaginalis Embley et al.

• Product format Freeze-dried

• 1. Open vial according to enclosed instructions.

Copyright and Trademark Information

© ATCC 2023. All rights reserved.

This information on this document was last updated on 2022-08-13

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