PLK LAWS FOUNDATION COLLEGE Section B:

Final Term Exam Mock (I)

(2021/2022)

Secondary: 3 Date: 6th July, 2022

Subject: Biology Time: 10:05 - 11:05

Full Mark: 90 Class: S3 _____ ( )

Paper: Question Paper Name: _________________

Instructions: 1. There are TWO sections in this examination paper:

Section A: Multiple-choice Questions (20 marks)

Section B: Structural Questions (45 marks)

2. When told to open this book, check that all the questions are there.
Look for ‘END OF PAPER’ after the last question.

3. Answer ALL the questions.

4. Write ALL answers in the spaces provided on the Answer Paper.

5. The diagrams in this paper are NOT necessarily drawn to scale.

FT\2021-2022\S3 Biology\Question Paper 1

Section A Multiple-choice Questions (20 marks)

1. The graph below shows the effect of temperature on the activity of salivary amylase:

Which of the following statements concerning salivary amylase is correct?

A. It is denatured at 0oC.
B. Its activity increases steadily with a rise in temperature.
C. It is most active at 60oC.
D. It is killed at 80oC.

2. A student defines osmosis as “the movement of water molecules from a dilute solution to a
concentrated solution across a selectively permeable membrane.” This definition is inaccurate because

A. solute molecules can also move along the concentration gradient.

B. water molecules can also move from the concentrated solution to the dilute solution.
C. it should state clearly that the membrane is not permeable to the solute molecules.
D. movement of water molecules can still occur without a selectively permeable membrane.

Questions 3 to 5 refer to the following experiments:

Two test tubes containing 5cm3 of hydrogen peroxide solution and 1 cm3 of catalase solution
respectively were kept at 30°C for 30 minutes. The contents of the two tubes were then mixed and
maintained at the same temperature, and the amount of oxygen released was recorded for 5 minutes. The
same experiment was repeated at 40°C. The results are shown below:

3. The initial rate of oxygen production at 40°C is higher than that at 30°C because

A. catalase is denatured at the lower temperature.

B. oxygen is less soluble at the higher temperature.
C. more active sites are present in the enzyme molecules at the higher temperature.
D. the substrate molecules have more kinetic energy at the higher temperature.

4. Which of the following is a correct interpretation of the graph?

A. At 30°C, the rate of reaction was higher at the 5th minute.

B. At both temperatures, the amount of oxygen released in 5 minutes was the same.
C. At both temperatures, the time for the decomposition of all hydrogen peroxide was the same.
D. All active sites of enzymes were occupied at the end of the experiment.
FT\2021-2022\S3 Biology\Question Paper 2
5. A third experiment was carried out as before but the contents were kept at 90°C. Which of the
following would best indicate the result of the experiment?

Question 6 to 7 refer to an experiment about osmosis.

Three strips of the same size were cut from a fresh potato and immersed into sucrose solutions of different
concentration for four hours. The experiment was repeated using a potato that has already been stored for a
certain period of time. The results obtained are shown in the table below:

Concentration of sucrose solution Percentage change in mass of the strips

(%) Fresh potato Stored potato
0 +20.0 +25.0
5 +6.0 +10.0
10 –7.0 –2.0

6. The dependent variable in the experiment is measured as the percentage changes in mass of potato
strips because

A. the final masses of the potato strips are different.

B. the initial masses of the potato strips are different.
C. there is a significant change in the mass of the photo strips.
D. the mass can be measured accurately by using an electronic balance.

7. Which of the following about the interpretation of the results is correct?

Water potential of potato

After storage Explanation
A. Decreases some starch is converted to sugars during storage
B. Decreases some starch is used for growing during storage
C. Increases water is lost through evaporation during storage
D. Increases the permeability of cell membrane increases during exercise

Questions 8 and 9 refer to the graph below.

It shows the results of an investigation of an enzyme reaction performed by a group of students.

FT\2021-2022\S3 Biology\Question Paper 3

8. Which of the following is the correct explanation of the above results?

A. More enzymes can be reused as the temperature increases.

B. Enzyme is made up of proteins, it is denatured as the temperature increases.
C. Enzyme collides with substrate more frequently as the temperature increases.
D. Enzyme is inactive at low temperature and therefore there is no reaction at 0°C.

9. After the investigation, one of the students concluded that the optimum temperature of this enzyme is
40°C. However, another student disagreed with this conclusion. We can verify whether the conclusion
is correct by repeating the experiment

A. several times.
B. with a wide range of temperature.
C. with a smaller interval of temperature.
D. with a greater amount of enzyme and substrate.

Questions 10 to 12 refer to the graph below.

It shows the rates of reactions catalysed by two enzyme A and B over a range of pH values:

10. The rates of the reactions catalysed by enzymes A and B are equal at

A. pH 4
B. pH 5
C. pH 6
D. pH 7.5

11. Which of the following is a correct interpretation of the graph?

A. Enzyme A is found in gastric juice.

B. Enzyme B is more active than enzyme A.
C. The optimum pH value for enzyme B is 1.8.
D. Enzyme A is more active than enzyme B at pH 4.

12. In the above study, it is important to keep the temperature at which the reactions occur constant
because

A. temperature affects enzyme activity.

FT\2021-2022\S3 Biology\Question Paper 4
 B. the enzyme becomes inactive at low temperatures.
C. the enzyme becomes denatured at low temperatures.
D. temperature should be the same for control experiments.

Questions 13 and 14 refer to an investigation on the enzyme catalase

The enzyme breaks down hydrogen peroxide and release oxygen. In order to show whether a certain plant
tissue contains catalase or not, Joyce set up the following experiment:

13. Joyce observed that a lot of gas bubbles were released in tube I but not in tube 2. In order to conclude
that the plant tissue contains catalase, what additional steps should be taken by Joyce?

(1) Test whether the gas bubbles contain oxygen

(2) Set up a tube containing water and the fresh plant tissue
(3) Set up a tube containing hydrogen peroxide but no plant tissue

A. (1) and (2) only

B. (1) and (3) only
C. (2) and (3) only
D. (1), (2) and (3)

14. When gas bubbles stopped coming out from tube I, Joyce added more hydrogen peroxide into the
tube. She observed that gas bubbles were produced again. What can be implied from this observation
about the property of enzymes?

A. Enzymes can be reused.

B. Enzymes are specific in action.
C. Enzymes are made up of proteins.
D. Enzymes can speed up chemical reactions.

Question 15 and 16 refer to the graph below

It shows the change in mass of a potato strip in distilled water at 20°C:

15. The rate of the osmosis is the highest between

FT\2021-2022\S3 Biology\Question Paper 5
 A. hour 0 and hour 0.5
B. hour 0.5 and hour 1.0
C. hour 1.0 and hour 1.5
D. hour 1.5 and hour 2.0

16. Which of the following is a correct description of the potato cells between hour 2 and hour 3?

A. The cells were turgid.

B. The cell vacuole burst.
C. The cell wall was impermeable to water.
D. The cell membrane was fully permeable.

Questions 17 and 18 to the experimental set-up shown below:

17. What will happen to the liquid levels in tubing (1) and (2) after one hour?

Tubing (1) Tubing (2)

A. Fall Fall
B. Fall Rise
C. Rise Rise
D. Rise Fall

18. Which of the following graphs shows the likely changes in liquid level in tubing (2) and (3)?

FT\2021-2022\S3 Biology\Question Paper 6

Questions 19 and 20 refer to the diagram below

It shows the initial condition of two set-ups:

19. After 1 hour, the liquid level in the capillary tube of set-up 2 is much higher than that of set-up 1.
Which of the following is a probable reason for this result?

A. Solution 1 has a lower water potential than solution 2.

B. The dialysis tubing of set-up 1 is less permeable to water than that of set-up 2.
C. Sugar is produced in solution 2.
D. Less starch is present in solution 2.

20. Which of the following treatments might increase the rate of rise of the liquid level in set-up 2?

(1) using a narrower dialysis tubing

(2) using a capillary tube with a smaller internal diameter
(3) raising the temperature of the distilled water from 20oC to 30oC

A. (1) and (2) only

B. (1) and (3) only
C. (2) and (3) only
D. (1), (2) and (3)

Section B Structural Questions (70 marks)

1. (a) Name all the cellular organelles which are surrounded by two layers of membrane.

(b) One of these organelles is concerned with energy production. Draw a simple labelled
diagram to show the structure of this organelle.

(c) How is the structure of the organelle in (b) related to its function in cellular metabolism?

(7 marks)

2. (a) Distinguish between diffusion and active transport. (4 marks)

(b) (i) Give three examples of body functions in mammals where active transport is essential.

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 (ii) Briefly describe the functional significance of active transport in absorbing salt in plant root
cell.

(iii) With reference to one of the functions you have mentioned in (i), describe how the
structure(s) concerned is/are structurally adapted to this function. (10 marks)

3. The graph below shows the change in cell volume of a certain type of animal cells when they were
put in a hypertonic salt solution:

(a) Name the process that causes the change in cell volume in phase I. (1 mark)

(b) Propose a possible explanation for the restoration of the cell volume in phase II. (3 marks)

4. An experiment was set up to study the effects of chemicals on the permeability of the cell
membrane of beet root. Beet root discs were prepared, rinsed with water and blotted dry. The same
number of beet root discs were placed into three test-tubes labelled A, B and C containing equal
volumes of fluids:

tube A contains water,

tube B contains 30% ethanol and,
tube C contains 30% sucrose solution.

Indicate from which test tube(s) the following observations were made during the course of the
experiment. Give your reasons.

(a) Beet root discs sank. The liquid in the test-tube looked intensely red.
(3 marks)

(b) Beet root discs floated initially and then sank. The liquid in the test tube looked the same in
colour as that in the control test-tube.
(4 marks)

FT\2021-2022\S3 Biology\Question Paper 8

 5. The following diagram is a model illustrating the structure of a plasma membrane:

(a) Name this model. Give two features that justify such a name.
(3 marks)
(b) Explain how the nature and arrangement of molecule at W are related to the permeability of the
membrane.
(4 marks)
(c) State the use of two types of membrane proteins respectively.
(2 marks)

6. A student performed an experiment to monitor the progress of a simple enzyme-catalysed reaction

involving one substrate and one product. He prepared replicate reaction tubes. In each tube, the
concentration of only one reaction component, either the substrate or product, was measured. The
results of three selected tubes are shown as curves A, B and C below:

(a) (i) Identify which measured component, substrate or product, is represented by each curve.
(2 marks)

(ii) It was realized that the reaction mixture in one of the three tubes had been wrongly
prepared. This resulted in a different reaction condition.

FT\2021-2022\S3 Biology\Question Paper 9

 (1) State which curve represents the result of an error in the preparation. Give a reason for your
choice. Suggest two possible mistakes in the student’s preparation of this reaction mixture.
(4 marks)

(2) What evidence shows that the other two curves represent identical reaction conditions?
(3 marks)

(iii) With reference to curve C,

(1) calculate the rate of enzyme reaction at the 3rd minute, given that 100% concentration is
equivalent to 100 mMoles of the measured component (Show the readings you take from the
graph in your calculation);
(2 marks)

(2) compare and explain the difference in enzyme reaction rate at the 3rd and 15th minute.
(3 marks)

(iv) Curve A will finally level off and will not reach 100 %. Explain this phenomenon.
(2 marks)
(b) Explain what is meant by the ‘active site’ of an enzyme.
(2 marks)

6. Compare and contrast the characteristics of competitive and non-competitive inhibitions on enzyme
activity.
(4 marks)

7. Give one application of enzyme and explain its effect.

(2 marks)
8. The following flowchart summarizes an experiment to determine whether compound X is an
inhibitor of amylase:

#erw

(a) Suggest a dependent variable to indicate the result of the experiment and suggest a test to
measure the dependent variable.
(2 marks)

FT\2021-2022\S3 Biology\Question Paper 10

 (b) Predict experimental result B is X is an inhibitor of amylase.
(1 mark)
(c) How will you modify the experiment to show that X is a competitive inhibitor of amylase?
(2 marks)
9.
(a) An experiment was conducted to investigate the effect of temperature on the activities of
amylases I and II. These enzymes were obtained from the same type of tissue of vertebrate
animals I and II respectively. Similar pH, enzyme concentration and substrate concentration
were used. The experimental data obtained are recorded in the following table:

Temperature/ Time for complete digestion of 1 unit of substrate/minute

℃
Amylase I Amylase II
10 2.0 5.0
15 1.6 3.5
20 1.5 2.5
25 1.6 2.0
30 2.0 1.6
35 4.0 1.4
40 10.0 1.5

(i) Plot a graph to demonstrate the effect of temperature on the activities of amylase I and II. 5 marks)

(ii) Explain the results for amylase I. (2 marks)

(iii)What are the optimum temperatures for amylase I and amylase II? What thermoregulatory ability
would the data for amylase suggest for vertebrate animal I? (2 marks)

(b) The graph below shows the effects of two chemicals, A and B, on the activity of amylase I. Similar pH,
temperature, enzyme concentration and chemical concentration were used in the experiment.

(i) Compare and contrast the effects of chemical A and chemical B on the activity of amylase I.
(3 marks)

(ii) Explain how each chemical, A and B, exerts its effect on amylase I.
(3 marks)

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