Taylor Fant - Diffusion Lab Data Sheet

Data Sheet for Diffusion Lab
Experiment 1: How Size and Temperature a ect di usion
1) What is the molecular weight of the two dyes used in this experiment?
• Potassium permanganate ___158.034_grams/mole____________
• Methylene blue ___319.85 grams/mole____________
2) Hypothesis: Which dye do you predict will di use the fastest (di use the
greatest distance in the hour)?
The potassium permanganate will di use the greatest distance because it weighs
less.
3) Record the data from the virtual lab video below:
Dye Distance traveled at 4°C (cm) Distance traveled at 22° C (cm)

Methylene blue .75 cm .85 cm
Potassium 1.1 cm 1.4 cm
permanganate
4) Calculate the rate of di usion for each dye at each temperature. (The rate of
di usion is the distance traveled divided by the me.)
Dye Rate of Di usion at 4°C in Rate of Di usion at 22° C in

minutes minutes
Methylene blue 0.75 0.85
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Potassium 1.1 1.4
permanganate
5) What conclusions can you draw about the e ect of the size of solute on the
rate of di usion based on this experiment?
The smaller the size of the solute, the faster the rate of di usion.
6) What conclusions can you draw about the e ect of temperature on the rate of
di usion based on this experiment?
The observa on suggests that the rate of di usion increases with an increase in temperature.
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Experiment 2: Di usion Across a Semipermeable Membrane
1) What do you know about the size of glucose and starch?

Starch molecules are made up of many molecules and are larger than glucose.
Therefore starch is less likely to di use across a semipermeable membrane than
glucose because of the di erence in size.
2) Hypothesis: Predict what you think will occur. Will both glucose and starch
be able to cross the semipermeable membrane (dialysis tubing)?
I think that both could cross the semipermeable membrane but glucose will pass
through quicker/easier due to its size.
Controls:
3) The Posi ve control for this experiment should contain
____starch_____________ and _____sugars____________.
4) The color result of this test with IKI was __posi ve___________.
5) The color result of this test with Benedict’s test was

____posi ve__________.
6) The Nega ve control for this experiment should contain ___no starch/
sugar____________.
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7) The result of this test with IKI was ____nega ve__________.
8) The result of this test with Benedict’s test was ____nega ve_________.
9) Complete the table below with the results from the test of the posi ve and
nega ve controls (steps 1 – 5 in the procedure.)
Controls Color a er Starch Color a er Glucose

IKI test present Benedict’s test present (yes/
(yes/no) no)
Posi ve Control Black Yes Orange Yes
Nega ve Control Orange No Blue No
10)At the beginning of the experiment, record the contents of the beaker and
the dialysis tubing. (water, starch, glucose)
Beaker Dialysis Tubing

What is contained Water Starch/glucose solu on
in the Beaker and
Dialysis Tubing at
the beginning of
the experiment?
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11)Complete the table below with the results a er 1 hour incuba on (steps 9 -
10 in the procedure).
Experimental Color a er Starch Color a er Glucose

Results IKI test present Benedict’s test present (yes/
(yes/no) no)
Sample from Nega ve No Orange Yes
Beaker
Sample from black Yes Red/ Orange Yes

dialysis tubing
12)Based on your results, which molecule was able to cross the semipermeable
membrane? How can you explain this?
Glucose was able to pass through the semipermeable membrane due to

its small size.
Experiment 3: Observa on of Osmosis in Plant Cells
1) A er viewing the video, include your observa ons of a plant cell in a

hypertonic solu on and in a hypotonic solu on. You can either sketch the
cells or include a brief descrip on of the characteris cs.
Plant Cell in a Hypertonic Solu on Plant Cell in a Hypotonic Solu on

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The chloroplast moves outward of the The chloroplast moves to the center of
cell, as the water moves in. the cell
2) How do animal and plant cells di er when placed in hypotonic solu ons?
Animal cells will shrivel up and die, whereas a plant cell with shrink but can
recover once returned to its isotonic or hypotonic solu on.
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Data Sheet for Diffusion Lab

Experiment 1: How Size and Temperature a ect di usion

• Methylene blue _319.85 grams/mole__________

greatest distance in the hour)?

3) Record the data from the virtual lab video below:

Dye Distance traveled at 4°C (cm) Distance traveled at 22° C (cm)

Dye Rate of Di usion at 4°C in Rate of Di usion at 22° C in

1) What do you know about the size of glucose and starch?

5) The color result of this test with Benedict’s test was

Controls Color a er Starch Color a er Glucose

Nega ve Control Orange No Blue No

Beaker Dialysis Tubing

Experimental Color a er Starch Color a er Glucose

Sample from black Yes Red/ Orange Yes

Glucose was able to pass through the semipermeable membrane due to

Experiment 3: Observa on of Osmosis in Plant Cells

1) A er viewing the video, include your observa ons of a plant cell in a

Plant Cell in a Hypertonic Solu on Plant Cell in a Hypotonic Solu on

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