Sop Gbt002 Rev 1 0
Sop Gbt002 Rev 1 0
Sop Gbt002 Rev 1 0
1.0 Purpose This procedure describes the correct method for hand washing in the Biotechnology laboratory. 2.0 Scope This procedure applies to all students, lab assistants and instructors who perform experimental work in the Biotechnology laboratory. 3.0 Responsibility Everyone who conducts experimental work in the Biotechnology laboratory is responsible for understanding and implementing this procedure when entering or exiting from the laboratory. 4.0 Procedure 4.1 Materials 4.1.1 Sink with hot and cold water 4.1.2 antimicrobial soap 4.1.3 clean paper towels 4.1.4 lab coat (cloth or disposable) 4.1.5 hair cover (optional) 4.2 Procedure 4.2.1 To be completed each time gloves are worn or removed - minimal at start and finish of laboratory work. 4.2.2 Remove all jewelry from fingers, thumbs and wrists. This includes removal of bracelets and watches or other wrist jewelry. 4.2.3 Put on lab coat and button closed. 4.2.4 Put on hair cover. 4.2.5 Check for paper towels and unroll or have towels ready 4.2.6 Turn on hot and cold water in sink. Water temperature should be hot but not so hot as to cause pain or burns. 4.2.7 Wet hands thoroughly. 4.2.8 Apply soap and lather. Be sure to wash each finger and thumb individually, wash up to and including several inches beyond the wrist area for a minimum of 1.5 minutes.
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SOP: GBT.002, Revision: 1.0 Title: Hand Washing Procedure for Biotechnology Labs
4.2.9
Rinse thoroughly and dry hands with clean paper towel. 4.2.10 Use the paper towel to turn off the water so that you do not have to touch the faucets with your now clean hands. 4.2.11 Dispose of the paper towel in the waste container, not in the sink! 5.0 Reference Biological Safety Principles and Practices 3rd edit. (2000). Pg 400. D.O. Fleming and D.L. Hunt (edits.) ASM Press, Washington D.C.
6.0 Validation of Process There are several possible methods: one is do a bacterial plate touch before and after washing your hands and count the number of colonies that form - hopefully there will be fewer after washing your hands. Another is to use a safe dye that mimics dirt there are some U.V. dyes available for this purpose and take a reading on the hands before and after washing - the procedure should remove the dye... note that either test procedure this should be done multiple times with several different people using the procedure - some variables to check would include length of time for lathering, brand of soap and brand of paper towels
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