SELECTRA - Vitalab

INDEX :

Code: Test: Method:

AMY Amylase – BPS BPS method

ACP Acid Phosphatase Fast red method
ALP Alkaline Phosphatase DGKC method
CPK CK – NAC Optimized kinetic method
CKMB CK – MB Immunological u.v.
GGT gamma – GT Szasz modified method
GOT GOT / AST IFCC method
GPT GPT / ALT IFCC method
LDH LDH SFBC method
CHOL Cholesterol CHOD – PAP method
DIRECT HDL -
HDL Enzymatic Direct method
Cholesterol
TRIG Triglycerides GPO – PAP method
CAL Calcium o-Cresolphthalein method
CA Calcium Arsenazo III
CL Chloride Thiocyanate method
FE Iron FerroZine method
MG Magnesium Xylidyl blue method
P Phosphorus Fiske-Subbarow method
ALB Albumin BCG method
TP Total Protein Biuret method
CRE Creatinine Modified Jaffe method
GLU Glucose Modified GOD – PAP
UREA Urea u.v. Urease – GLDH method
UAR Uric Acid Modified Uricase – PAP
BIT Automatic Bilirubin Jendrassik-Grof method
ASO ASO Latex 1/5 Turbidimetric method
CRP CRP Latex 1/5 Turbidimetric method
RF RF Latex 1/5 Turbidimetric method
CHE Cholinesterase Butyrylthiocoline method
LIP Lipase Colour Coulorimetric method

QUIMICA CLINICA APLICADA S.A.

A 7 Km 1081 – PO Box 20 - E43870 AMPOSTA / SPAIN Empresa certificada / Certified
Tel. ++ 34 (977) 70. 14. 39 Fax ++ 34 (977) 70. 30. 40 ISO 9001:2000 - ISO 13485:2003
Revision: February 2013
 SELECTRA - Vitalab

Parameters Test
Test name: AMY ACP ALP CPK CK MB GGT
Mode: Kinetic Kinetic Kinetic Kinetic 2 POINTS Kinetic
Linearity limit: 15 % 15 % 15 % 15 % 15 % 15 %
Delay: 60 sec. 180 sec. 70 sec. 60 sec. 180 sec. 70 sec.
Incubation: 60 sec. 180 sec. -- 129 sec. 480 sec. 30 sec.
1st Point: -- -- -- -- -- --
2nd Point: -- -- -- -- -- --
Wavelength: 405 nm 405 nm 405 nm 340 nm 340 nm 405 nm
Units: U/L U/L U/L U/L U/L U/L
Nº of decimal: 0 1 0 0 0 0
Sample volume: 8 µl 25 µl 5 µl 10 µl 13 µl 24 µl
Rerun samp. vol: 5 µl 5 µl 2 µl 2 µl 10 µl 10 µl
Reagent vol.: 300 µl 300 µl 250 µl 250 µl 300 µl 250 µl
Rerun reag. vol: 301 µl 350 µl 253 µl 258 µl 320 µl 253 µl
Low Abs: 0.100 Abs 0.100 Abs -0.100 Abs 0.000 Abs 0.100 Abs -0.100 Abs
High Abs: 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs
Low conc.: -- -- -- -- -- --
High conc.: -- -- -- -- -- --
React. deviation: 3.000 Abs 0.200 Abs 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs
React. low.limit: 0.150 Abs 0.050 Abs 0.250 Abs -0.100 Abs 0.050 Abs 0.100 Abs
React. high limit: 2.000 Abs 0.900 Abs 2.800 Abs 2.000 Abs 0.900 Abs 2.000 Abs
Reagent blank: SI SI SI SI SI SI
Sample blank: No No No No No No
Calibrator: -- -- -- -- -- --
Repetition: -- -- -- -- -- --
Nº: -- -- -- -- -- --
Concentration: -- -- -- -- -- --
Low limit: -- -- -- -- -- --
High limit: -- -- -- -- -- --
Factor: 3950 743 2760 4127 1651 1158

* Must introduce the calibrator concentration

** This value will be calculated

QUIMICA CLINICA APLICADA S.A.

A 7 Km 1081 – PO Box 20 - E43870 AMPOSTA / SPAIN Empresa certificada / Certified
Tel. ++ 34 (977) 70. 14. 39 Fax ++ 34 (977) 70. 30. 40 ISO 9001:2000 - ISO 13485:2003
Revision: February 2013
 SELECTRA - Vitalab

Parameters Test
Test name: GOT GPT LDH CHOL HDL TRI
Mode: Kinetic Kinetic Kinetic End Point End Point End Point
Linearity limit: 15 % 15 % 15 % -- -- --
Delay: 70 sec. 70 sec. 32 sec. -- -- --
Incubation: -- -- -- 11.5 min 6.5 min 11.5 min
1st Point: -- -- -- -- -- --
2nd Point: -- -- -- -- -- --
Wavelength: 340 nm 340 nm 340 nm 505 nm 620 nm 505 nm
Units: U/L U/L U/L mg/dl mg/dl mg/dl
Nº of decimal: 0 0 0 0 1 0
Sample volume: 25 µl 25 µl 5 µl 3 µl 2 µl 4 µl
Rerun samp. vol: 5 µl 5 µl 2 µl 2 µl 1 µl 3 µl
Reagent vol.: 250 µl 250 µl 250 µl 300 µl Dual:240- 300 µl
80µl
Rerun reag. vol: 270 µl 253 µl 253 µl 301 µl Dual:241-80µl 301 µl
Low Abs: 0.400 Abs 0.400 Abs 0.400 Abs -- 0.000 Abs --
High Abs: 3.000 Abs 3.000 Abs 3.000 Abs -- 3.000 Abs --
Low conc.: -- -- -- 0 mg/dl 0 mg/dl 0 mg/dl
High conc.: -- -- -- 750 mg/dl 200 mg/dl 1000 mg/dl
React. deviation: 0.300 Abs 3.000 Abs 3.000 Abs -- -- --
React. low.limit: -0.100 Abs -0.100 Abs -0.100 Abs -0.100 Abs -0.010 Abs -0.100 Abs
React. high limit: 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs
Reagent blank: SI SI SI SI SI SI
Sample blank: No No No No Si No
Calibrator: -- -- -- Multicalibrator STD-HDL Multicalibrator
Repetition: -- -- -- 1 2 1
Nº: -- -- -- 1 1 1
Concentration: -- -- -- * * *
Low limit: -- -- -- -0.100 0.000 -0.100
High limit: -- -- -- 3.000 3.000 3.000
Factor: -1746 -1746 -8095 ** ** **

* Must introduce the calibrator concentration

** This value will be calculated

QUIMICA CLINICA APLICADA S.A.

A 7 Km 1081 – PO Box 20 - E43870 AMPOSTA / SPAIN Empresa certificada / Certified
Tel. ++ 34 (977) 70. 14. 39 Fax ++ 34 (977) 70. 30. 40 ISO 9001:2000 - ISO 13485:2003
Revision: February 2013
 SELECTRA - Vitalab

Parameters Test Arsenazo III

Test name: CA CAL CLO FE P MG
Mode: End Point End Point End Point End Point End Point End Point
Linearity limit: -- -- -- -- -- --
Delay: -- -- -- -- -- --
Incubation: 6.5 min 6 min 6 min 11.5 min 11.5 min 6 min
1st Point: -- -- -- -- -- --
2nd Point: -- -- -- -- -- --
Wavelength: 546 nm 630 nm 492 nm 578 nm 630 nm 505 nm
Units: mg/dl mg/dl mEq/L g/dl mg/dl mg/dl
Nº of decimal: 2 2 0 0 1 1
Sample volume: 4 µl 8 µl 3 µl 24 µl 12 µl 3 µl
Rerun samp. vol: 3 µl 5 µl 2 µl 8 µl 10 µl 2 µl
Reagent vol.: 300 µl 300 µl 300 µl 240 µl 300 µl 300 µl
Rerun reag. vol: 301 µl 301 µl 301 µl 248 µl 301 µl 301 µl
Low Abs: -- -- -- -- -- --
High Abs: -- -- -- -- -- --
Low conc.: 0 mg/dl 0 mg/dl 0 mEq/l 0 mg/dl 0 mg/dl 0 mg/dl
High conc.: 20 mg/dl 25 mg/dl 175 mEq/l 1000 g/dl 15 mg/dl 7 mg/dl
React. deviation: -- -- -- -- -- --
React. low.limit: -0.100 Abs -0.100 Abs -0.100 Abs -0.100 Abs -0.100 Abs -0.100 Abs
React. high limit: 2.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs
Reagent blank: SI SI Si Si Si Si
Sample blank: No No No No No No
Calibrator: Multicalibrator Multicalibrator Multicalibrator Multicalibrator Multicalibrator Multicalibrator
Repetition: 1 1 1 1 1 1
Nº: 1 1 1 1 1 1
Concentration: * * * * * *
Low limit: -0.100 -0.100 -0.100 -0.100 -0.100 -0.100
High limit: 2.000 3.000 3.000 3.000 3.000 3.000
Factor: ** ** ** ** ** **

* Must introduce the calibrator concentration

** This value will be calculated

QUIMICA CLINICA APLICADA S.A.

A 7 Km 1081 – PO Box 20 - E43870 AMPOSTA / SPAIN Empresa certificada / Certified
Tel. ++ 34 (977) 70. 14. 39 Fax ++ 34 (977) 70. 30. 40 ISO 9001:2000 - ISO 13485:2003
Revision: February 2013
 SELECTRA - Vitalab

Parameters Test
Test name: ALB CRE GLU PTO URE URA
Mode: End Point 2 Points End Point End Point 2 Points End Point
Linearity limit: -- -- -- -- -- --
Delay: -- -- -- -- -- --
Incubation: 6 min -- 11.5 min 11.5 min -- 11.5 min
1st Point: -- 32 sec. -- -- 32 sec. --
nd
2 Point: -- 90 sec. -- -- 129 sec. --
Wavelength: 620 nm 505 nm 505 nm 546 nm 340 nm 546 nm
Units: g/dl Mg/dl mg/dl g/dl mg/dl mg/dl
Nº of decimal: 1 1 0 1 1 1
Sample volume: 2 µl 25 µl 3 µl 5 µl 4 µl 10 µl
Rerun samp. vol: 1 µl 8 µl 2 µl 3 µl 5 µl 2 µl
Reagent vol.: 300 µl 220 µl 300 µl 250 µl 300 µl 250 µl
Rerun reag. vol: 301 µl 242 µl 301 µl 252 µl 301 µl 253 µl
Low Abs: -- -- -- -- -- --
High Abs: -- -- -- -- -- --
Low conc.: 0 mg/dl 0 mg/dl 0 mg/dl 0 g/dl 0 mg/dl 0 mg/dl
High conc.: 7 g/dl 20 mg/dl 600 mg/dl 15 g/dl 300 mg/dl 22 mg/dl
React. deviation: -- 3.000 Abs -- -- 3.000 Abs --
React. low.limit: -0.100 Abs -0.100 Abs -0.100 Abs -0.100 Abs 0.400 Abs -0.100 Abs
React. high limit: 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs
Reagent blank: Si SI SI Si SI SI
Sample blank: No No No No No No
Calibrator: Multicalibrator Multicalibrator Multicalibrator Multicalibrator Multicalibrator Multicalibrator
Repetition: 1 1 1 1 1 1
Nº: 1 1 1 1 1 1
Concentration: * * * * * *
Low limit: -0.100 0 dAbs -0.100 -0.100 0 -0.100
High limit: 3.000 2.300 dAbs 3.000 3.000 2.500 dAbs 3.000
Factor: ** ** ** ** ** **

* Must introduce the calibrator concentration

** This value will be calculated

QUIMICA CLINICA APLICADA S.A.

A 7 Km 1081 – PO Box 20 - E43870 AMPOSTA / SPAIN Empresa certificada / Certified
Tel. ++ 34 (977) 70. 14. 39 Fax ++ 34 (977) 70. 30. 40 ISO 9001:2000 - ISO 13485:2003
Revision: February 2013
 SELECTRA - Vitalab

Parameters Test
Test name: BIT ASO CRP RF CHE LIPASE
Mode: End Point 2 Points 2 Points 2 Points Kinetic Kinetic
Linearity limit: -- 800 150 140 15 % 15 %
Delay: -- -- -- -- 12 sec. 120 sec.
Incubation: 11.5 min -- -- -- -- --
1st Point: -- 32 sec. 32 sec. 32 sec. -- --
2nd Point: -- 90 sec. 90 sec. 180 sec. -- --
Wavelength: 505 nm 546 nm 546 nm 620 nm 405 nm 578 nm
Units: mg/dl IU/ml mg/l U/ml U/L U/L
Nº of decimal: 0 0 1 0 0 1
Sample volume: 30 µl 5 µl 3 µl 7 µl 3 µl 4 µl
Rerun samp. vol: 32 µl 2 µl 2 µl 3 µl 2 µl 2 µl
Reagent 1 vol.: 300 µl 300 µl 300 µl 300 µl 397 µl 200 µl
Rerun reag. vol: 301 µl 301 µl 301 µl 301 µl 398 µl 200 µl
Reagent 2 vol.: -- 120 µl
Low Abs: -- 0.000 Abs. 0.000 Abs. 0.000 Abs. -0.100 Abs -0.100 Abs
High Abs: 0 mg/dl 3.000 Abs. 3.000 Abs. 3.000 Abs. 3.000 Abs 3.000 Abs
Low conc.: 25 mg/dl 5 U/ml 0.5 mg/Ll 5 U/ml -- 0.000
High conc.: -- 800 U/ml 150 U/ml 140 U/ml -- 250.0
React. deviation: -0.100 Abs 3.000 3.000 3.000 3.000 3.000
React. low.limit: 3.000 Abs 0.000 Abs -0.100 Abs -0.100 Abs 0.000 Abs 0.000 Abs
React. high limit: SI 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs 3.000 Abs
Reagent blank: No SI SI SI SI SI
Sample blank: Multicalibrator No No No No No
Calibrator: 1 STD STD STD Yes Yes
Repetition: 1 1 1 1 1 1
Nº: * 1 1 1 ** **
Concentration: * 250 60 75
Low limit: -0.100 -0.100 -0.100 -0.100
High limit: 2.000 3.000 3.000 3.000
Factor: ** -- -- -- 10025+ **

QUIMICA CLINICA APLICADA S.A.

A 7 Km 1081 – PO Box 20 - E43870 AMPOSTA / SPAIN Empresa certificada / Certified
Tel. ++ 34 (977) 70. 14. 39 Fax ++ 34 (977) 70. 30. 40 ISO 9001:2000 - ISO 13485:2003
Revision: February 2013