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    A Brief Notes On Chromatography and Separation Techniques

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    A Brief Notes On Chromatography and Separation Techniques

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    A Brief Notes On Chromatography and Separation Techniques

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    OPEN ACCESS Freely available online

    Journal of Chromatography & Separation Techniques


    Commentary

    A Brief Notes on Chromatography and Separation Techniques


    Susanne Haze*
    Department of Chemistry, University of Nebraska, USA

    COMMENTARY molecules that are flowing with a flow rate through the column.
    Molecules larger than pores cannot permeate into gel particles
    Chromatography is the most widely used technique that involves and are retained within a particular area. Larger molecules pass
    the separation, identification, of component mixture for both through the pores and move rapidly inside the column.
    qualitative and quantitative analysis. Proteins can be separated based
    on size, shape, and total charge, hydrophobic groups present on the The affinity chromatography technique is most widely used for
    surface. Based on the hydrophobic groups present on the surface the purification of enzymes hormones, antibiotics, nucleic acids,
    there are different types of chromatographic techniques: Column and specific proteins. A ligand that can make a complex with
    chromatography, Ion-exchange chromatography, gel-permeation specific protein binds with the filling material of the column. By
    (molecular sieve) chromatography, affinity chromatography, using this High-pressure liquid chromatography we can perform
    paper chromatography, thin-layer chromatography, gas the structural and functional analysis of many molecules within
    chromatography, dye-ligand chromatography, hydrophobic a short time. The high-pressure liquid chromatography technique
    interaction chromatography, pseudoaffinity chromatography, yields perfect results in the separation and identification of
    high-pressure liquid chromatography (HPLC). The purpose of carbohydrates, lipids, amino acids, nucleic acids, proteins,
    applying chromatography is a method of quantitative analysis of steroids, and other biologically active molecules in the HPLC
    separation based on two phases: a stationary phase and a mobile mobile phase passes through columns under 10-400 atmospheric
    phase. The type of interaction between the stationary phase and pressure, and with a high flow rate.
    mobile phase and the substances contained in the mixture is the
    Chromatographic techniques were used to separate substances
    most effective component in separating molecules from each
    based on color and pigments. With time its application area
    other.
    was extended considerably nowadays chromatography is a widely
    Ion exchange chromatography is based on electrostatic interaction accepted effective separation technique. Chromatography is one
    between two charged protein groups and a solid support matrix of the useful separations and determination methods. column
    .matrix has an ion load opposite to that of the protein to be chromatography is a protein purification method realized based
    separated .proteins are separated by changing ph concentration on the characteristic features of proteins. Besides these methods
    of ion salts or ionic strength of buffer solution .positively charged HPLC has many features in its higher sensitivity, rapid turnover
    ion exchange matrices are called anion-exchange matrices and rate, its use as a quantitative method can purify amino acids,
    they will absorb negatively charged proteins in case if the matrices proteins, nucleic acids, hydrocarbons, carbohydrates, drugs,
    bound with negatively charged groups are called cation exchange antibiotics, and steroids.
    matrices and absorb positively charged proteins. The principle
    involved in Gel-permeation (molecular sieve) chromatography ACKNOWLEDGEMENT
    method is to use dextran-containing materials to separate The author is very thankful to all the associated personnel in any
    macromolecules based on their differences in molecular sizes. reference that contributed in/for the purpose of this research.
    This process is used to determine the molecular weights of
    proteins and to decrease the salt concentrations of protein CONFLICT OF INTEREST
    solutions. In a gel permeation column, the stationary phase
    consists of inert molecules with small pores the solution contains The author has declared that no competing interests exist..

    Correspondence to: Susanne Haze, Department of Chemistry, University of Nebraska, USA; E-mail: [email protected]
    Received: November 04, 2021; Accepted: November 18, 2021; Published: November 25, 2021
    Citation: Susanne H (2021) A Brief note on Chromatography and Separation Techniques. J Chromatogr Sep. 12:462
    Copyright: © Susanne H. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
    unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    J Chromatogr Sep Tech., Vol.12 Iss.11.462


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