HACH FF-1A Fish Farming Water Quality Test Kit

DOC326.97.
00098
Fish Farming Water Quality Test Kit

FF-1A (243002)
11/2017, Edition 1
User Manual
Table of Contents
General information .................................................................................................. 3
Safety information ........................................................................................................ 3
Use of hazard information ............................................................................................ 3
Product overview ......................................................................................................... 3
Product components .................................................................................................... 4
Alkalinity ........................................................................................................................ 5
Test preparation ........................................................................................................... 5
Test procedure—Alkalinity, HR (gpg CaCO3) ............................................................. 5
Test procedure—Alkalinity, LR (gpg CaCO3) .............................................................. 6
Determine the alkalinity relationships .......................................................................... 6
Replacement items ...................................................................................................... 7
Optional items .............................................................................................................. 7
Ammonia ........................................................................................................................ 8
Test preparation ........................................................................................................... 8
Test procedure—Ammonia-nitrogen (0–3.0 mg/L NH3–N) ......................................... 8
Calculate the mg/L NH3 ............................................................................................... 9
Replacement items .................................................................................................... 11
Optional items ............................................................................................................ 11
Carbon dioxide .......................................................................................................... 12
Test preparation ......................................................................................................... 12
Test procedure—Carbon dioxide (0–100 mg/L CO2) ................................................ 12
Optional items ............................................................................................................ 13
Chloride ........................................................................................................................ 14
Test procedure—Sodium chloride (0–2500 mg/L NaCl) ............................................ 14
Test procedure—Sodium chloride (0–500 mg/L NaCl) .............................................. 15
Optional items ............................................................................................................ 15
Dissolved oxygen ..................................................................................................... 16
Test procedure—Dissolved oxygen (0–20 mg/L O2) ................................................. 16
Test procedure—Dissolved oxygen (0.2–4 mg/L O2) ................................................ 17
Optional items ............................................................................................................ 19
Hardness, Total ......................................................................................................... 20
Test procedure—Hardness (0–20 gpg CaCO3) ........................................................ 20
Optional items ............................................................................................................ 21
Nitrite ............................................................................................................................. 22
Test procedure—Nitrite-nitrogen LR (0–0.4 mg/L NO2––N) ..................................... 22
1
Table of Contents
Optional items ............................................................................................................ 23
pH .................................................................................................................................... 24
Test procedure—pH (4–10 pH units) ......................................................................... 24
Optional items ............................................................................................................ 25
Supersaturation ........................................................................................................ 26
Test procedure—Supersaturation .............................................................................. 26
2
General information
In no event will the manufacturer be liable for direct, indirect, special, incidental or consequential
damages resulting from any defect or omission in this manual. The manufacturer reserves the right to
make changes in this manual and the products it describes at any time, without notice or obligation.
Revised editions are found on the manufacturer’s website.
Safety information
NOTICE
The manufacturer is not responsible for any damages due to misapplication or misuse of this product including,
without limitation, direct, incidental and consequential damages, and disclaims such damages to the full extent
permitted under applicable law. The user is solely responsible to identify critical application risks and install
appropriate mechanisms to protect processes during a possible equipment malfunction.
Please read this entire manual before unpacking, setting up or operating this equipment. Pay
attention to all danger and caution statements. Failure to do so could result in serious injury to the
operator or damage to the equipment.
Make sure that the protection provided by this equipment is not impaired. Do not use or install this
equipment in any manner other than that specified in this manual.
Use of hazard information

DANGER
Indicates a potentially or imminently hazardous situation which, if not avoided, will result in death or serious injury.
WARNING
Indicates a potentially or imminently hazardous situation which, if not avoided, could result in death or serious
injury.
CAUTION
Indicates a potentially hazardous situation that may result in minor or moderate injury.
NOTICE
Indicates a situation which, if not avoided, may cause damage to the instrument. Information that requires special
emphasis.
Product overview
The Fish Farming Water Quality Test Kit includes the necessary items to measure important
parameters in aquaculture. Refer to Table 1.
Some measurement methods use reagents and color discs to make a visual determination of the
parameter concentration. Refer to Figure 1. Other measurement methods use reagents and titration
procedures to measure the parameter concentration.
Table 1 Test kit parameters
Parameter Range Method
Alkalinity 0–100, 0–400 mg/L CaCO3 Titration
Ammonia 0–3.0 NH3–N Color disc
Carbon dioxide 0–100 mg/L CO2 Titration
Chloride 0–150, 0–600 mg/L Cl– Titration
Dissolved oxygen 0–4, 0–20 mg/L O2 Titration
Hardness, total 0–342 mg/L CaCO3 Titration
English 3
Table 1 Test kit parameters (continued)
Parameter Range Method
Nitrite 0–0.4 mg/L NO2–N Color disc
pH 4–10 pH units Color disc
Supersaturation Qualitative Observation
Figure 1 Color comparator box
1 Windows for color matching 4 Scale window

2 Left opening for viewing tube 5 Color disc
3 Right opening for viewing tube
Product components
Make sure that all components have been received. Refer to the list that follows. If any items are
missing or damaged, contact the manufacturer or a sales representative immediately.
• Bottle, mixing • Dissolved Oxygen 1 Powder Pillows

• Bottle, BOD, 60 mL • Dissolved Oxygen 2 Powder Pillows
• Carrying case • Dissolved Oxygen 3 Powder Pillows (4x)
• Clippers • Hardness 1 Buffer Solution
• Color comparator box • Hardness 2 Indicator Solution
• Color discs (3x) • Hardness 3 Titrant Solution
• Color viewing tubes, plastic (2x) • NitriVer3 Powder Pillows
• Dropper • Nessler Reagent Solution
• Thermometer (Item number 2676400) • Phenolphthalein Reagent Solution
• Tube, plastic 5.83 mL • Rochelle Salt Solution
• Bromcresol Green-Methyl Red Powder Pillows • Sodium Thiosulfate Reagent Solution
• Chloride 2 Indicator Reagent Powder Pillows • Sulfuric Acid Reagent Solution, 0.030 N
• Silver Nitrate Titrant Solution • Sodium Hydroxide Solution, 0.1 N
• Wide-range pH Indicator Solution
4 English
Alkalinity
Test preparation
CAUTION
Review the Safety Data Sheets (MSDS/SDS) for the chemicals that are used. Use the recommended personal
protective equipment.
• Hold the dropper vertically above the sample. Do not let the dropper touch the bottle during the
titration.
• Rinse the tubes and bottles with sample before the test. Rinse the tubes and bottles with deionized
water after the test.
• Alkalinity is the capacity of water to neutralize acids. Carbonates, bicarbonates and hydroxides are
the primary sources of alkalinity in water.
• To verify the test accuracy, use a standard solution as the sample.
• To record the test result as mg/L CaCO3, multiply the test result in gpg by 17.1.
Test procedure—Alkalinity, HR (gpg CaCO3)
1. Fill the measuring 2. Pour the sample into 3. Add one drop of 4. Add the Sulfuric Acid
tube with sample. the mixing bottle. Phenolphthalein Standard Solution by
Indicator Solution. Swirl drops. Mix after each
to mix. drop. Count the drops
If the solution is until the color changes
colorless, the from pink to colorless.
Phenolphthalein (P)
alkalinity is zero. Go to
step 6.
5. Record the number 6. Add one Bromcresol 7. Add the Sulfuric Acid 8. Add the number of
of drops. The number of Green-Methyl Red Standard Solution by drops from step 5 and
drops is the Powder Pillow. Swirl to drops. Mix after each step 7 to get the total
phenolphthalein mix. drop. Count the drops (methyl orange)
alkalinity result as gpg until the color changes alkalinity result as gpg
CaCO3. from green to pink. CaCO3.
English 5
Test procedure—Alkalinity, LR (gpg CaCO3)
1. Fill the bottle to the 2. Add one drop of 3. Add the Sulfuric Acid 4. Divide the number of
15‑mL mark with Phenolphthalein Standard Solution by drops by 2.5 to get the
sample. Indicator Solution. Swirl drops. Mix after each phenolphthalein
to mix. drop. Count the drops alkalinity result as gpg
If the solution is until the color changes CaCO3.
colorless, the from pink to colorless.
Phenolphthalein (P)
alkalinity is zero. Go to
step 5.
5. Add one Bromcresol 6. Add the Sulfuric Acid 7. Add the number of 8. Divide the total
Green-Methyl Red Standard Solution by drops from step 3 and number of drops by 2.5
Powder Pillow. Swirl to drops. Mix after each step 6. to get the total (methyl
mix. drop. Count the drops orange) alkalinity result
until the color changes as gpg CaCO3.
from green to pink.
Determine the alkalinity relationships

The primary forms of alkalinity in water are hydroxide, carbonate and bicarbonate ions. The
concentration of these ions in a sample can be determined from the phenolphthalein alkalinity and
total alkalinity values. Refer to Table 2 and the steps that follow to determine the hydroxide,
carbonate and bicarbonate alkalinities.
1. If the phenolphthalein (P) alkalinity is 0 mg/L, use Row 1.

2. If the phenolphthalein (P) alkalinity is equal to the total alkalinity, use Row 2.
3. Divide the total alkalinity by 2 to calculate one-half of the total alkalinity.
a. Compare the phenolphthalein (P) alkalinity to one-half of the total alkalinity. Then, use Row 3,
4 or 5.
b. Do the calculations in the row (if applicable).
4. Make sure that the sum of the three alkalinity types is equal to the total alkalinity.
Example:
A sample has 170 mg/L as CaCO3 phenolphthalein alkalinity and 250 mg/L as CaCO3 total
alkalinity.
6 English
The phenolphthalein alkalinity of 170 mg/L is more than one-half of the total alkalinity, so use
Row 5.
• Hydroxide alkalinity: 2 x 170 = 340; 340 – 250 = 90 mg/L hydroxide alkalinity

• Carbonate alkalinity: 250 – 170 = 80; 80 x 2 = 160 mg/L carbonate alkalinity
• Bicarbonate alkalinity: 0 mg/L
Sum of the alkalinity types: 90 mg/L hydroxide alkalinity + 160 mg/L carbonate alkalinity + 0 mg/L
bicarbonate alkalinity = 250 mg/L total alkalinity.
Table 2 Alkalinity relationships
Row Titration result Hydroxide alkalinity Carbonate alkalinity Bicarbonate alkalinity
1 P alkalinity = 0 0 0 = Total alkalinity
2 P alkalinity = Total alkalinity = Total alkalinity 0 0
3 P alkalinity is less than ½ of 0 = P alkalinity × 2 = Total alkalinity – (P

Total alkalinity alkalinity × 2)
4 P alkalinity = ½ Total 0 = Total alkalinity 0

alkalinity
5 P alkalinity is more than ½ = (P alkalinity × 2) – = (Total alkalinity – P 0

Total alkalinity Total alkalinity alkalinity) × 2
Replacement items
Note: Product and Article numbers may vary for some selling regions. Contact the appropriate distributor or refer to
the company website for contact information.
Description Unit Item no.
Bromcresol Green-Methyl Red Indicator Powder Pillows 100/pkg 94399
Phenolphthalein Indicator Solution, 1 g/L 15 mL SCDB 189736
Sulfuric acid standard solution, 0.030 N 100 mL MDB 2620532
Bottle, square, 29 mL, with 10, 15, 20 and 23-mL marks 6/pkg 232706
Measuring tube, plastic, 5.83 mL each 43800
Optional items
Alkalinity standard solution, 500 mg/L as CaCO3 1L 2826253
Water, deionized 500 mL 27249
English 7
Ammonia
Test preparation
CAUTION
• Put the color disc on the center pin in the color comparator box (numbers to the front).
• Use sunlight or a lamp as a light source to find the color match with the color comparator box.
• Rinse the tubes with sample before the test. Rinse the tubes with deionized water after the test.
• If the color match is between two segments, use the value that is in the middle of the two
segments.
• If the color disc becomes wet internally, pull apart the flat plastic sides to open the color disc.
Remove the thin inner disc. Dry all parts with a soft cloth. Assemble when fully dry.
• The recommended sample temperature is 20 °C. Warmer temperatures cause high results. Colder
temperatures cause low results.
• More than 6 gpg hardness causes a white precipitate to develop. To remove the interference, add
1 drop of Rochelle salt reagent to the sample tube before the reagent is added.
• To measure very low quantities of ammonia, add ammonia-free water to one tube. Add 3 drops of
the Nessler reagent. Put this tube in the left opening of the color comparator box as the reagent
blank.
• To record the test result as mg/L NH3, multiply the test result by 1.2. To record the test result as
mg/L NH4+, multiply the test result by 1.3.
Test procedure—Ammonia-nitrogen (0–3.0 mg/L NH3–N)
1. Fill two tubes to the 2. Put one tube into the 3. Add 3 drops of 4. Swirl to mix. A
first line (5 mL) with left opening of the color Nessler reagent to the yellow color develops.
sample. comparator box. second tube.
5. Wait 1 minute. Read 6. Put the second tube 7. Hold the color 8. Read the result in
the result within into the color comparator box in front mg/L in the scale
5 minutes. comparator box. of a light source. Turn window.
the color disc to find the
color match.
8 English
Calculate the mg/L NH3
Ammonia in water is in the form of the ammonium ion (NH4+) and un-ionized ammonia (NH3). NH3 is
toxic to fish. Table 3 shows that the percent of NH3 increases as the pH and temperature increase.
This test kit measures NH4+ and NH3 as ammonia nitrogen (NH3–N).
To calculate the mg/L NH3 in the sample, refer to Table 3 and the equation that follows.
mg/L NH3 = ((mg/L NH3–N x percent NH3 from Table 3) ÷ 100) × 1.2
Example: The test result is 1.6 mg/L NH3–N. The sample pH is 7.6 and the sample temperature is
16 °C. The mg/L NH3 is ((1.6 x 1.16) ÷ 100) × 1.2 = 0.02 mg/L NH3.
To calculate the mg/L NH4+ in the sample, refer to Table 3 and the equation that follows.
mg/L NH4+ = ((mg/L NH3–N x (100 – percent NH3 from Table 3)) ÷ 100) × 1.3
Example: The test result is 1.6 mg/L NH3–N. The sample pH is 7.6 and the sample temperature is
16 °C. The mg/L NH4+ is ((1.6 x (100 – 1.16)) ÷ 100) × 1.3 = 2.056 mg/L NH4+.
English 9
Table 3 Percent of NH3 in water
pH 4 °C 6 °C 8 °C 10 °C 12 °C 14 °C 16 °C 18 °C 20 °C 22 °C 24 °C 26 °C 28 °C 30 °C 32 °C
7.0 0.11 0.13 0.16 0.18 0.22 0.25 0.29 0.34 0.39 0.46 0.52 0.60 0.69 0.80 0.91
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7.2 0.18 0.21 0.25 0.29 0.34 0.40 0.46 0.54 0.62 0.82 0.83 0.96 1.10 1.26 1.44
7.4 0.29 0.34 0.40 0.46 0.54 0.63 0.73 0.85 0.98 1.14 1.31 1.50 1.73 1.98 2.26
7.6 0.45 0.53 0.63 0.73 0.86 1.00 1.16 1.34 1.55 1.79 2.06 2.36 2.71 3.10 3.53
7.8 0.72 0.84 0.99 1.16 1.35 1.57 1.82 2.11 2.44 2.81 3.22 3.70 4.23 4.82 5.48
8.0 1.13 1.33 1.56 1.82 2.12 2.47 2.86 3.30 3.81 4.38 5.02 5.74 6.54 7.43 8.42
8.2 1.79 2.10 2.45 2.86 3.32 3.85 4.45 5.14 5.90 6.76 7.72 8.80 9.98 11.29 12.72
8.4 2.80 3.28 3.83 4.45 5.17 5.97 6.88 7.90 9.04 10.31 11.71 13.26 14.95 16.78 18.77
8.6 4.37 5.10 5.93 6.88 7.95 9.14 10.48 11.97 13.61 15.41 17.37 19.50 21.78 24.22 26.80
8.8 6.75 7.85 9.09 10.48 12.04 13.76 15.66 17.73 19.98 22.41 25.00 27.74 30.62 33.62 36.72
9.0 10.30 11.90 13.68 15.65 17.82 20.18 22.73 25.46 28.36 31.40 34.56 37.83 41.16 44.53 47.91
9.2 15.39 17.63 20.08 22.73 25.58 28.61 31.80 35.12 38.55 42.04 45.57 49.09 52.58 55.99 59.31
9.4 22.38 25.33 28.47 31.80 35.26 38.84 42.49 46.18 49.85 53.48 57.02 60.45 63.73 66.85 69.79
9.6 31.36 34.96 38.38 42.49 46.33 50.16 53.94 57.62 61.17 64.56 67.77 70.78 73.58 76.17 78.55
9.8 42.00 46.00 50.00 53.94 57.78 61.47 64.99 68.31 71.40 74.28 76.92 79.33 81.53 83.51 85.30
10.0 53.44 57.45 61.31 64.98 68.44 71.66 74.63 77.35 79.83 82.07 84.08 85.88 87.49 88.92 90.19
10.2 64.53 68.15 71.52 74.63 77.46 80.03 82.34 84.41 86.25 87.88 89.33 90.60 91.73 92.71 93.58
Replacement items
Nessler reagent 100 mL MDB 2119432
Color disc, ammonia nitrogen, Nessler, 0–3.0 mg/L each 9262600
Color comparator box each 173200
Plastic viewing tubes, 18 mm, with caps 4/pkg 4660004
Optional items
Caps for plastic viewing tubes (4660004) 4/pkg 4660014
Glass viewing tubes, 18 mm 6/pkg 173006
Rochelle salt solution 29 mL DB 172533
Stoppers for 18-mm glass tubes and AccuVac Ampuls 6/pkg 173106
English 11
Carbon dioxide
Test preparation
CAUTION
titration.
• Dissolved carbon dioxide is in surface waters at concentrations less than 10 mg/L. Ground water
can have higher concentrations. Fish may tolerate high concentrations of carbon dioxide if
dissolved oxygen concentrations are also high. Fish usually stay away from areas where the
concentration of carbon dioxide is high. The relationship of carbon dioxide to fish respiration and
photosynthesis causes daily fluctuations in the CO2 concentration. The concentration usually
increases during the night and decreases during the day. High concentrations of carbon dioxide
may become toxic to fish when dissolved oxygen levels are very low.
Test procedure—Carbon dioxide (0–100 mg/L CO2)
1. Fill the measuring 2. Pour the sample into 3. Add one drop of the 4. Turn the bottle left
tube with sample. the mixing bottle. Phenolphthalein and right to mix.
Indicator Solution.
5. Add the Sodium 6. Multiply the number

Hydroxide Solution by of drops of the sodium
drops. Mix after each hydroxide solution by 5
drop. Count the drops to get the result in
until a pink color stays mg/L.
for 30 seconds.
Replacement items
12 English
Phenolphthalein Indicator Solution, 1 g/L 15 mL SCDB 189736
Sodium hydroxide standard solution, 0.01 N 100 mL MDB 67132
Optional items
English 13
Chloride
Test preparation
CAUTION
• The chloride concentration in fish waters can be low or high. The concentration is usually higher
near coastal regions. Measure chloride before and after salt is added to fish waters.
titration.
• Keep the silver nitrate titrant solution in the closed test kit case when not in use. The titrant
solution slowly decomposes in light.
• To record the test result as mg/L chloride (Cl–), multiply the NaCl result by 0.6.
• To verify the test accuracy, use a standard solution as the sample. Make sure to change the test
result to mg/L chloride if the standard solution concentration is given as mg/L chloride (Cl–).
Test procedure—Sodium chloride (0–2500 mg/L NaCl)
1. Fill the measuring 2. Pour the sample into 3. Add one Chloride 2 4. Turn the bottle left
tube with sample. the mixing bottle. Indicator Powder Pillow. and right to mix.
5. Add the Silver 6. Multiply the total

Nitrate Titrant Solution number of drops by 50
by drops. Mix after each to get the result in
drop. Count the drops mg/L.
until the color changes
to red-brown.
14 English
Test procedure—Sodium chloride (0–500 mg/L NaCl)
1. Fill the bottle to the 2. Add one Chloride 2 3. Turn the bottle left 4. Add the Silver
23‑mL mark with Indicator Powder Pillow. and right to mix. Nitrate Titrant Solution
sample. by drops. Mix after each
drop. Count the drops
until the color changes
to red-brown.
5. Multiply the total

number of drops by
12.5 to get the result in
mg/L.
Replacement items
Chloride 2 Indicator Powder Pillows 100/pkg 104399
Chloride titrant, silver nitrate solution 100 mL MDB 39732
Optional items
Chloride standard solution, 1000 mg/L Cl– 500 mL 18349
English 15
Dissolved oxygen
Test preparation
CAUTION
titration.
• Air bubbles cause incorrect results. To prevent air bubbles below the stopper, tilt the bottle and tap
the stopper quickly on the bottle neck. Look below the stopper to make sure that there are no air
bubbles.
• Keep the sodium thiosulfate away from direct sunlight.
• If the sample contains high concentrations of chloride (e.g., sea water) the floc that develops in the
bottle does not fall. Wait 4 or 5 minutes after the floc develops, then continue the test.
• If the high-range procedure gives a low result, use the prepared sample for the titration in the low-
range procedure.
• Measure the dissolved oxygen (DO) concentration frequently to help monitor the health of fish and
other organisms. The primary source of oxygen in fish waters comes from photosynthesis by
phytoplankton. The dissolved oxygen concentration changes with fluctuations in the temperature,
sunlight, atmospheric pressure, salinity, plant life and water turbulence.
• For better visibility of the titration endpoint, use starch indicator solution. Titrate the sample until
the color starts to change from yellow-brown to light yellow, then add 2 drops of starch indicator
solution. Continue the titration until the color changes from blue to colorless.
Test procedure—Dissolved oxygen (0–20 mg/L O2)
1. Hold the DO bottle 2. Tap the stopper 3. Add one Dissolved 4. Immediately put the
with the stopper in the quickly on the bottle Oxygen 1 Powder stopper on the bottle.
sample water. Remove neck. Remove the Pillow and one Make sure that no air
the stopper and let the bottle from the water. Dissolved Oxygen 2 bubbles are below the
bottle fill to the top. Make sure that no air Powder Pillow. stopper. Shake the
Keep the bottle in the bubbles are trapped in bottle vigorously.
water. the bottle.
16 English
5. A brown-orange floc 6. Shake the bottle 7. Remove the stopper. 8. Immediately put the
develops. The floc again. Wait until the top Add one Dissolved stopper on the bottle.
slowly falls. Wait until half of the bottle is Oxygen 3 Powder Shake the bottle. The
the top half of the bottle clear. Pillow. floc dissolves and a
is clear. yellow color develops.
9. Fill the measuring 10. Add the Sodium 11. Record the number
tube with the prepared Thiosulfate solution by of drops. The number of
sample. Pour the drops. Mix after each drops of the titrant
prepared sample into drop. Count the drops solution is equal to the
the mixing bottle. until the solution is result in mg/L.
colorless.
Test procedure—Dissolved oxygen (0.2–4 mg/L O2)
1. Hold the DO bottle 2. Tap the stopper 3. Add one Dissolved 4. Immediately put the
with the stopper in the quickly on the bottle Oxygen 1 Powder stopper on the bottle.
sample water. Remove neck. Remove the Pillow and one Make sure that no air
the stopper and let the bottle from the water. Dissolved Oxygen 2 bubbles are below the
bottle fill to the top. Make sure that no air Powder Pillow. stopper. Shake the
Keep the bottle in the bubbles are trapped in bottle vigorously.
water. the bottle.
English 17
5. A brown-orange floc 6. Shake the bottle 7. Remove the stopper. 8. Immediately put the
develops. The floc again. Wait until the top Add one Dissolved stopper on the bottle.
slowly falls. Wait until half of the bottle is Oxygen 3 Powder Shake the bottle. The
the top half of the bottle clear. Pillow. floc dissolves and a
is clear. yellow color develops.
9. Pour the prepared 10. Add the Sodium 11. Multiply the number
sample from the bottle Thiosulfate solution by of drops of the titrant
until the volume in the drops. Swirl to mix after solution by 0.2 to get
bottle is 30 mL. each drop. Count the the result in mg/L.
drops until the color
changes to colorless.
Replacement items
Dissolved Oxygen 1 Reagent Powder Pillows, 60 mL 100/pkg 98199
Dissolved Oxygen 2 Reagent Powder Pillows, 60 mL 100/pkg 98299
Dissolved Oxygen 3 Reagent Powder Pillows 100/pkg 98799
Sodium Thiosulfate Standard Solution, stabilized, 0.0109 N 100 mL MDB 2408932
Clippers each 96800
Bottle, BOD, 60 mL, with stopper each 190902
18 English
Optional items
Starch Indicator Solution 100 mL MDB 34932
Stopper, ground-glass for BOD bottles each 190901
English 19
Hardness, Total
Test preparation
CAUTION
titration.
• To record the test result as mg/L CaCO3, multiply the test result in gpg by 17.1.
Test procedure—Hardness (0–20 gpg CaCO3)
1. Fill the measuring 2. Pour the sample into 3. Add three drops of 4. Turn the bottle left
tube with sample. the mixing bottle. the Hardness 1 Buffer and right to mix.
Solution.
5. Add one drop of the 6. Turn the bottle left 7. Add the Hardness 3 8. Record the number
Hardness 2 Indicator and right to mix. Titrant Solution by of drops. The number of
Solution. A pink color drops. Mix after each drops of the titrant
develops. drop. Count the drops solution is the result in
until the color changes gpg.
from pink to blue.
Replacement items
Hardness 1 Buffer Solution 100 mL MDB 42432
Hardness 2 Indicator Solution 100 mL MDB 42532
Hardness 3 Titrant Solution 100 mL MDB 42632
20 English
Replacement items (continued)
Optional items
Standard solution, hardness (20 gpg) and iron (2 mg/L) 500 mL 47949
English 21
Nitrite
Test preparation
CAUTION
segments.
• Undissolved reagent does not have an effect on test accuracy.
• If the sample contains more than 0.5 mg/L nitrite-nitrogen, dilute the sample as follows. Use the
dropper to add 1 mL of sample to each tube. Dilute the sample to the 5-mL mark with deionized
water. Use the diluted sample in the test procedure and multiply the result by 5.
• To record the test result as mg/L NO2–, multiply the test result by 3.33.
• Nitrite-nitrogen develops during the biological decomposition of organic nitrogen compounds.
Nitrites are quickly oxidized to nitrates and are not usually found in surface waters. Levels of nitrite
more than the natural residual amounts can be acutely poisonous to fish.
Test procedure—Nitrite-nitrogen LR (0–0.4 mg/L NO2––N)
1. Fill two tubes to the 2. Put one tube into the 3. Add one NitriVer 4. Put a cap on the
first line (5 mL) with left opening of the color 3 Nitrite Reagent tube. Shake for
sample. comparator box. Powder Pillow to the 1 minute. A pink color
second tube. develops.
5. Wait 10 minutes. 6. Put the second tube 7. Hold the color 8. Read the result in
Read the result within into the color comparator box in front mg/L in the scale
15 minutes. comparator box. of a light source. Turn window.
color match.
22 English
Replacement items
NitriVer 3 Nitrite Reagent Powder Pillows, 5 mL 100/pkg 1407899
Color disc, nitrite nitrogen, 0–0.4 mg/L each 9262300
Optional items
Dropper, glass, 0.5- and 1.0-mL marks 5/pkg 1419705
English 23
pH
Test preparation
CAUTION
segments.
• To verify the test accuracy, use a buffer solution as the sample.
• Chlorine can interfere with the test. To remove chlorine from the sample, add 1 drop of sodium
thiosulfate solution to 5 mL of sample and mix. Use this dechlorinated sample in the test
procedure. One drop of the sodium thiosulfate removes a maximum of 50 mg/L chlorine from the
sample.
• The pH of water is a measure of the hydrogen ion concentration on a scale of 0 (very acidic) to
14 (very alkaline), with pH 7 being neutral. The pH of most natural waters ranges from pH 4 to pH
9. Dissolved carbon dioxide, carbonates, bicarbonates and acid rain cause changes in the pH.
Phytoplankton and other aquatic plant life remove carbon dioxide from the water during
photosynthesis, which causes the pH to increase during the day. To make an estimate of the pH
cycle of a body of water, make pH measurements at different times of the day. The best pH values
for fish production measured at sunrise are approximately 6.5 to 9. The acid and alkaline death
points for most fish are approximately pH 4 and pH 11.
Test procedure—pH (4–10 pH units)
1. Fill two tubes to the 2. Put one tube into the 3. Add 6 drops of wide 4. Swirl to mix.
first line (5 mL) with left opening of the color range pH indicator
sample. comparator box. solution to the second
tube.
24 English
5. Put the second tube 6. Hold the color 7. Read the result in
into the color comparator box in front pH units in the scale
comparator box. of a light source. Turn window.
color match.
Replacement items
Wide range pH indicator solution 100 mL MDB 2329332
Color disc, pH, wide range each 990100

Sodium Thiosulfate Standard Solution, stabilized, 0.0109 N 100 mL MDB 2408932
Optional items
English 25
Supersaturation
Test preparation
• Supersaturation occurs when more air dissolves in water than is soluble, which can cause gas
bubble disease in fish.
• The temperature of the collected sample must not change for accurate results.
• Be careful to not trap air bubbles during sample collection. To prevent air bubbles during sample
collection, tilt the bottle and tap the stopper quickly on the bottle neck. Look below the stopper to
make sure that there are no air bubbles.
Test procedure—Supersaturation
1. Soak the DO bottle 2. Hold the DO bottle 3. Tap the stopper 4. Look for gas bubbles
in the sample water with the stopper in the quickly on the bottle during a period of
until the bottle is the sample water. Remove neck. Keep the bottle in several hours. Gas
same temperature as the stopper and let the the water so that the bubbles are an
the sample water. bottle fill to the top. temperature does not indication that the water
Keep the bottle in the change. is supersaturated with
water. gas.
Replacement items
Bottle, BOD, 60 mL, with stopper each 190902
Stopper, ground-glass for BOD bottles each 190901
26 English
*DOC326.97.00098*
HACH COMPANY World Headquarters HACH LANGE GMBH HACH LANGE Sàrl
P.O. Box 389, Loveland, CO 80539-0389 U.S.A. Willstätterstraße 11 6, route de Compois
Tel. (970) 669-3050 D-40549 Düsseldorf, Germany 1222 Vésenaz
(800) 227-4224 (U.S.A. only) Tel. +49 (0) 2 11 52 88-320 SWITZERLAND
Fax (970) 669-2932 Fax +49 (0) 2 11 52 88-210 Tel. +41 22 594 6400
[email protected] [email protected] Fax +41 22 594 6499
www.hach.com www.de.hach.com
© Hach Company/Hach Lange GmbH, 2017.

All rights reserved. Printed in U.S.A.

HACH FF-1A Fish Farming Water Quality Test Kit

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DOC326.97.

Fish Farming Water Quality Test Kit

Use of hazard information

Alkalinity 0–100, 0–400 mg/L CaCO3 Titration

Ammonia 0–3.0 NH3–N Color disc

Carbon dioxide 0–100 mg/L CO2 Titration

Chloride 0–150, 0–600 mg/L Cl– Titration

Dissolved oxygen 0–4, 0–20 mg/L O2 Titration

Hardness, total 0–342 mg/L CaCO3 Titration

Nitrite 0–0.4 mg/L NO2–N Color disc

pH 4–10 pH units Color disc

Supersaturation Qualitative Observation

Figure 1 Color comparator box

1 Windows for color matching 4 Scale window

• Bottle, mixing • Dissolved Oxygen 1 Powder Pillows

Test procedure—Alkalinity, HR (gpg CaCO3)

Determine the alkalinity relationships

1. If the phenolphthalein (P) alkalinity is 0 mg/L, use Row 1.

• Hydroxide alkalinity: 2 x 170 = 340; 340 – 250 = 90 mg/L hydroxide alkalinity

1 P alkalinity = 0 0 0 = Total alkalinity

2 P alkalinity = Total alkalinity = Total alkalinity 0 0

3 P alkalinity is less than ½ of 0 = P alkalinity × 2 = Total alkalinity – (P

4 P alkalinity = ½ Total 0 = Total alkalinity 0

5 P alkalinity is more than ½ = (P alkalinity × 2) – = (Total alkalinity – P 0

Description Unit Item no.

Bromcresol Green-Methyl Red Indicator Powder Pillows 100/pkg 94399

Phenolphthalein Indicator Solution, 1 g/L 15 mL SCDB 189736

Sulfuric acid standard solution, 0.030 N 100 mL MDB 2620532

Measuring tube, plastic, 5.83 mL each 43800

Description Unit Item no.

Alkalinity standard solution, 500 mg/L as CaCO3 1L 2826253

Water, deionized 500 mL 27249

Test procedure—Ammonia-nitrogen (0–3.0 mg/L NH3–N)

Description Unit Item no.

Nessler reagent 100 mL MDB 2119432

Color disc, ammonia nitrogen, Nessler, 0–3.0 mg/L each 9262600

Color comparator box each 173200

Plastic viewing tubes, 18 mm, with caps 4/pkg 4660004

Description Unit Item no.

Caps for plastic viewing tubes (4660004) 4/pkg 4660014

Water, deionized 500 mL 27249

Glass viewing tubes, 18 mm 6/pkg 173006

Rochelle salt solution 29 mL DB 172533

Test procedure—Carbon dioxide (0–100 mg/L CO2)

5. Add the Sodium 6. Multiply the number

Phenolphthalein Indicator Solution, 1 g/L 15 mL SCDB 189736

Sodium hydroxide standard solution, 0.01 N 100 mL MDB 67132

Measuring tube, plastic, 5.83 mL each 43800

Description Unit Item no.

Water, deionized 500 mL 27249

Test procedure—Sodium chloride (0–2500 mg/L NaCl)

5. Add the Silver 6. Multiply the total

5. Multiply the total

Description Unit Item no.

Chloride 2 Indicator Powder Pillows 100/pkg 104399

Chloride titrant, silver nitrate solution 100 mL MDB 39732

Measuring tube, plastic, 5.83 mL each 43800

Description Unit Item no.

Water, deionized 500 mL 27249

Chloride standard solution, 1000 mg/L Cl– 500 mL 18349