Fume Hood

3Q Doc(IQ,OQ, PQ)
 Content

1 Introduction

2 Group members

3 Purpose

4 Range

5 Tested contents

5.1 Installation confirmation

5.1.1 Items in installation confirmation

5.1.2 Records of installation confirmation

5.2 Operation confirmation

5.2.1 Confirmation items in operation

5.2.2 Records in operation

5.3 Performance confirmation

5.3.1 Items in performance confirmation

5.3.2 Records in performance confirm

6 Docs and tools needed for verification

7 Deviation and relative treatment

8 Confirmed result

9 Confirmed comments and suggestions

 Fume Hood 3Q plan
1 Introduction
Fume hood is an indispensable part of laboratory. There shall be good
ventilation in the laboratory in order to prevent laboratory workers from inhaling or
swallowing toxic chemicals and organisms that may cause disease or whose toxicity is
unknown.To prevent the absorption of some vapors, gases, and particulates (smoke,
soot, dust, and aerosols), pollutants must be removed by means of fume hood
ventilation.The structure of the fume hood is up and down, and the top of the fume hood
is equipped with exhaust air hole, which can be used to install fans.

2 Group members
Name Dept Work

3. Purpose
This file’s purpose is to confirm if it can meet approved design and customer’s requirement that Fume

hood and the relative control system’s installation, operation and performance. And it can meet all the

requirements in China.

4 Range
Installation confirmation, operational confirmation and performance confirmation are to

ascertain that the Fume hood can meet relative standards and customer’s requirements.

5 Tested contents
5.1 Installation confirmation
5.1.1 Items in installation confirmation
No. Items Requirement Method Tool According to

1 Installation Check if it is in User direct the Tape Qualified if it is in

position accordance with installation at site, measure accordance with

confirmatio installation position in to measure the customer

n the drawing as positioning at site. confirmation or with

confirmed with drawing confirmed.

customer Dimensional

tolerance,refer to

GB/T1804-2000

China（IT16 Grade）

2 Environmen Biosafety cabinet need Check with Temperatur Temp：10℃～30℃

tal condition to meet the BSL-1to temperature and e and Humidity：≤70%

BSL-3 laboratory humidity meter humidity Address: no strenuous

requirements, and meter shaking, no light and

environment no sunshine

should maintain

constant temperature,

humidity and high

degree of cleanliness

3 Outlook No cracking, no rust, no 1. Use eye to see 1. Outlook has no

pressing mark. Surface inner and outer obvious damage

is smooth and flat. surface 2. Material and

Functional letters and 2. Use eye to accessory are

signs are clear check material same with

and accessory drawing required

4 Size Check if it is produced Use measure tape Tape Outside dimension

checking according to confirmed to check each size measure and work area

size dimension should

 meet design

requirement.

Dimensional

difference refer to

GB/T 1804-2000

（IT16 grade）

5 Air flow The airflow in the fume Test by eyes：Fog Smoke

pattern hood should be inward proof at the generator

operating exit

5.1.2 Records of installation confirmation

No. Items Result Conclusion

1 Installation After installation, the booth condition

position Site confirmation
confirmatio Levelling adjustment confirmation
n Firmness of bolts, nuts and other fixing
parts
2 Temp：10℃～30℃
Environmen Relative humidity：≤70%
tal condition
Address: no strenuous shaking, no light and
no sunshine
3 Surface of cabinet (Includes front window)
have no scratching, no hollows,no
cracking,no rust, no pressing mark and any
other obvious flaws.
Nameplate,signboard,label etc.these logo
parts should be installed uprightly in a right
place. Words,marks,patterns etc. should be
right and clear.
Outlook
Welding parts are fixed and flat.Splicing
Checking
place have no obviously slits,the sealed
place should be gluded in a right way.
The opening and closing of the front
window should be light, and there should be
no jamming at any position within the travel
range. There should be no obvious shaking
from left to right or back and forth, and the
sliding should be smooth.
 The structure of sliding front window
should ensure that it cannot fall off when the
suspension system fails, which will bring
danger to the operator.
4 Outside size and inner size should meet
Size
design requirements of GB/T 1804-2000 中
Checking
（IT16 class）
5 The airflow in the fume hood should be
Air flow
inward
Comprehensive opinions：

5.2 Operation confirmation

5.2.1 Confirmation items in operation

No. Items Requirement Method Tool According to

1 Motion Satisfying Using the manual，operate Each part of operations
Checking normal rightly, check working are normal.
operation ,an condition and sequence.
d confirm
working
sequence .
2 Noise Checking 1. Use sound level meter to Sound level Noise≤70dB(A)
noise. test in front of the center meter
of the Biosafety
Cabinet upright 300mm
and 380mm above the
operating
zone.(Picture3)
2. During it working, test
the actual noise value.
3. Off machine,test the
surrounding noise value
as background noise.
4. When the difference
between actual noise and
background noise is
below 9dB(A), it needs
to be compensated. The
compensation value
 will as the mesured
value.
Adjusted according to
follows:
When the difference between
actual noise value and
background noise value＜
3dB(A), the test is invalid.
When the
differences=3dB(A), test
invalid.
When the difference is
between 4 and
6dB(A),compensation value
is -2
When the difference is
between 7 and
9dB(A),compensation value
is -1
When the differences
≥10dB(A),no compensating.

3 Inflow Identify Open the front window to Electric hot The average velocity of
velocity whether the the maximum height, take bulb inflow airflow:
velocity of points parallel to the front anemometer standard
inflow window, divide into 4 rows (Adjusted value±0.025m/s；
airflow is on average, 4 points in each within one
consistent row year)
with
standard
value.
4 Illuminatio Test the The convenient Illuminomet Average illumination of
n illumination illuminometer is used to er Biosafety Cabinet is no
of work evenly arrange points along more than 4001x.
zone the center of the two inner
walls of the work table. The
distance between measuring
points is no more than
300mm, and the minimum
distance from the side wall is
150mm. The average value is
taken as the illuminance of
the fume hood.
5 Airflow Test the Smoke at the front window Flow The air should flow
 pattern airflow opening visualizatio inward
pattern when n instrument
the
equipment is
on normal
operation.

6 Withstand Electrical The equipment is in a Voltage No breakdown

voltage test safety test non-working state, the power tester
switch is placed in the put
position, and the dielectric
strength of the zero line to
the ground is tested by the
voltage tester.

5.2.2 Records in Operation

No. Items Result Conclusion
1 Motion Check the action
detection sequence of
structure
(reliability of
sliding door etc.)
Operation
sequence
inspection of
electrical
appliances (wind
speed alarm
function, etc.)
2 Noise Test 300mm
outward the
center horizontal
of the dispensing
booth , 380mm
above the ground
of work zone
3 Inflow Open the front 1 Average=
velocity window to the
maximum height,
2
take points
parallel to the
front window, 3
divide into 4
 rows on average, 4
4 points in each
row;Downdraft
velocity (mean
wind speed）
n

 xi / n
i=1
=

4 Illumination Evenly arrange 1 Average=

points along the
center of the two 2
inner walls of the
work table. The 3
distance between
measuring points 4
shall not exceed
300mm, and the 5
minimum
distance from the
side wall shall be
150mm. Take the
average value as
the illumination
of the fume hood
5 Airflow Smoke around
pattern the front window
of the fume hood

8 Withstand The equipment is

voltage test in a non-working
state, the power
switch is placed
in the put
position, and the
dielectric
strength of the
zero line to the
ground is tested
by the voltage
tester.
Consolidated comments：

5.3 Performance confirmation

5.3.1 Items in performance confirmation

No. Items Requiremen Method Tool According to

t
1 Inflow Confirm Open the front window to the Hot-bulb Inflow Average
Velocity whether it is maximum height, take points anemomete Velocity:Nominal
consistent parallel to the front window, r（Adjusted Value±0.025m/s
with the divide into 4 rows on average, 4 within one
standards. points in each row year)

5.3.2 Records in performance confirm

No. Items Result Conclusion
1 Inflow Open the front 1 Average=
Velocity window to the
maximum height,
2
take points
parallel to the
front window, 3
divide into 4 rows
on average, 4 4
points in each row
（Average wind
n
speed）=  xi / n
i=1

Consolidated comments：
6 Documents and tools needed for verification

No. Document name Note

1 Technical manual of Fume Hood

2 Standard operating procedure for fume hoods

No. Equipment and tools Note

1 Tape measure

2 Temp and humidity meter

3 Spectrophotometer/Illuminometer

4 Heated thermometer

5 Smoke generator

6 Sound level meter

7 Flow visualization instrument

8 Withstanding Voltage Tester

7 Deviation and relative treatment

When the deviation occurs, the reason for the deviation should be analyzed and the suggestions for

dealing with it should be put forward.

8 Confirmed result
The group leader verifies the conclusion of the validation, and report to the team leader to ratify.

9 Confirmed comments and suggestions

Team leader gives some evaluations and suggestions for the verification report.