USER MANUAL

Revision K

Alliance Bio Expertise – 19 Rue Louis Blériot 35170 – Bruz

+33 (0)2 40 51 79 53 – [email protected] 1
 Table of contents
Pictograms used in the manual. ............................................................................ 6
Disposal and recycling........................................................................................... 6
Warranty ............................................................................................................... 8
General Safety Instructions ................................................................................... 8
Risks and Prevention ............................................................................................. 9
Use of PPE .......................................................................................................... 10
Discharging wastewater ...................................................................................... 10
Installation and Commissioning .......................................................................... 11
Unpacking.......................................................................................................................... 11
Inventory of MEDIAWEL 30/50 parts and accessories ............................................ 11
Environment for Installing your MEDIAWEL.......................................................... 12
Overall dimensions: ......................................................................................................... 12
Site constraints ................................................................................................................ 13
Atmosphere ...................................................................................................................... 13
Water supply ..................................................................................................................... 13
Power supply .................................................................................................................... 13
First Commissioning ........................................................................................... 14
Connecting to the power supply ................................................................................... 14
Connecting to the water supply .................................................................................... 15
Connecting to the water drainage ................................................................................ 16
Switching on and off the device .................................................................................... 16
Filling the double jacket .................................................................................................. 16
Presentation ........................................................................................................ 17
Overview ............................................................................................................................ 17
Rear view ........................................................................................................................... 18
Lid details .......................................................................................................................... 19
Vessel inside ..................................................................................................................... 20
Touch screen .................................................................................................................... 21
Status Indicators .............................................................................................................. 22
Device Parameters .............................................................................................. 23
Language / Date and time setting ................................................................................ 23
Managing Users .................................................................................................. 24
Creating a user ................................................................................................................. 24
Modifying a user............................................................................................................... 25
Deleting a user .................................................................................................................. 26
Activating the access rights management ........................................................... 27
Managing programs ............................................................................................ 29
Creating a program.......................................................................................................... 29
Modifying a program ....................................................................................................... 34
Deleting a program .......................................................................................................... 35
Running a Cycle................................................................................................... 36
Preparation........................................................................................................................ 36
Starting a Cycle ................................................................................................................ 39
Dispensing......................................................................................................................... 41
Interrupting a cycle .......................................................................................................... 43
Cleaning .............................................................................................................. 44
Cleaning between cycles ................................................................................................ 45
Maintenance and Servicing.................................................................................. 48
Summary table ................................................................................................................. 48
Temperature probe adjustment .................................................................................... 49
Descaling the device ....................................................................................................... 50
Troubleshooting ............................................................................................................... 52
Icons ................................................................................................................................... 52
Messages .......................................................................................................................... 52
Technical Specifications ...................................................................................... 54
 Declaration of Conformity MEDIAWEL30
ALLIANCE BIO EXPERTISE, Bruz, France
Declares that the here below material complies with the following European directives.
Description Models
MEDIAWEL 30 MEDW1001
comply with

EU Directives :
Pressure Equipment 2014/68/EU
CE – CEM 2004/108/CE

Harmonized standards applied EN61326-1:2013

EN610010-2-10:2014
IEC61010-1: 2010

Material resistance calculation according: ASME VIII div 1 Edition 2013

ASME II Part D Edition 2013

Directive 2014/68/EU
Description of pressure vessel:
Operational fluid liquids/gases
Fluid group 2
Category (2014/68/EU) I
Max operating pressure 1.4 bar
Safety valve set pressure, Max allowable pressure PS 1.7 bar
Allowable temperature TS 0-125 °C
Volume 37 l
Test pressure (water) 2.5 bars
140405-21-
Drawing No./Rev 100rev05

The declaration has been issued by the manufacturer

The person responsible of the declaration is:
Name, : LE SAUX Philippe
Position : President Alliance Bio Expertise sas

Bruz, March the 21th 2023

 Declaration of Conformity MEDIAWEL50
ALLIANCE BIO EXPERTISE, Bruz, France
Declares that the here below material complies with the following european directives.
Description Models
MEDIAWEL 50 MEDW1050
comply with

EU Directives :
Pressure Equipment 2014/68/EU
CE - CEM 2004/108/CE

Harmonized standards applied EN61326-1:2013

EN610010-2-10:2014
IEC61010-1: 2010
Material resistance calculation according : ASME VIII div 1 Edition 2013
ASME II Part D Edition 2013

Directive 2014/68/EU
Description of pressure vessel :
Operational fluid liquids/gases
Fluid group 2
Category (2014/68/EU) I
Max operating pressure 1.4 bar
Safety valve set pressure, Max allowable pressure PS 1.7 bar
Allowable temperature TS 0-125 °C
Volume 57 l
Test pressure (water) 2.5 bars
PLN220224-1-1 rev 00
Drawing No./Rev

The declaration has been issued by the manufacturer.

The person responsible of the declaration is:
Name, : LE SAUX Philippe
Position : President Alliance Bio Expertise

Bruz, March the 21th 2023

 Pictograms used in the manual.
To make this manual easier to read, the following symbols indicate specific information:

This symbol indicates a prerequisite prior to an action, or operation.

This symbol indicates an informative message.

This symbol indicates an important message for the device good operating
order.

This symbol indicates an important message concerning the user’s safety.

Disposal and recycling

This device is composed of metal, plastic materials, electric components

and electronic components.
The 2002/96/CE directive on recovering and recycling Waste from Electric
and Electronic Equipment (WEEE) requires Electric and Electronic Equipment
(EEE) to be recycled at the end of its life.
Therefore, end users must dispose of electric and electronic waste at a waste
management facility or a dedicated collection site.
For more information about returns, please contact the equipment
manufacturer.

6
Document History:

Version Date Modifications

K JUNE 2023 Content homogenisation within ABE technical documents

Due to the informative nature of the information and diagrams contained in this manual,
ALLIANCE BIO EXPERTISE reserves the right to make changes to the document without
prior notice.

7
 Warranty

ALLIANCE BIO EXPERTISE hereby certifies that the device displays the characteristics set out
in this manual on delivery. Machines are guaranteed against manufacturing defects and
defective components for a period of twelve months from the dispatch date.

ALLIANCE BIO EXPERTISE shall carry out possible repairs of components, within its premises,
during the warranty period. All components or devices experiencing any kind of malfunction
must be returned to the ALLIANCE BIO EXPERTISE factory at the cost of ALLIANCE BIO
EXPERTISE's representative or the client, if necessary.

The warranty shall not be valid for issues relating to transport, accidents, incorrect use or
internal interference caused by a poor-quality electricity network or people who are not
authorized to use the device.

General Safety Instructions

The MEDIAWEL is exposed to high temperatures and pressures during sterilization

cycles. For this reason, it is essential to follow the safety instructions in this operating
manual.

• Never use the automated preparator to sterilize hazardous materials or products,

which contain alkalis. Sterilization of these products may cause an explosion,
corrosion of the chamber or chamber pipework and damage to seals.

• Should a liquid containing salt water and high salinity agar-saline agar, etc. be used,
you must rinse the vessel well after use and wipe the latter's surfaces as well as
around the cover seal (corrosion risk).

• Do not modify the device. Any modifications made by personnel not trained and
authorized by ALLIANCE BIO EXPERTISE immediately exclude the responsibility of
ALLIANCE BIO EXPERTISE

• The MEDIAWEL must undergo annual preventive maintenance, carried out by

trained personnel authorized by ALLIANCE BIO EXPERTISE.

• Defective parts must only be replaced with original spare parts supplied
directly or indirectly by ALLIANCE BIO EXPERTISE.

8
Risks and Prevention

Means of prevention and

Symbol Risk Cause Component
Instructions
• Thermal insulation of
components
Lid
Hot • Lid and hatch locked during
Bowl
surface • the working cycle
Do not touch hot surfaces
• Wear EPP
Burns
• No access above 80°C
Hot steam Safety valve
• Wear EPP

• Safety valve
Dispensing
• Release the steam before
Splashing and adding
opening the lid by using the
port caps
safety valve

Water Safety valve • No access above 80°C

Burns
steam Caps • Wear EPP

• Counterbalance springs
Lid
Pinching Lid weight • Hold the lid during closing.

Electric Short-circuit Power supply The device must be plugged to a

shock grounded electrical outlet.

9
 Use of PPE
When HMI screen displays an alarm, it is mandatory to wear the following safety equipment:
• Heatproof safety apron
• Heatproof gloves
• Waterproof safety shoes
• Safety visors

A prevention sticker is supplied with the device. Please display it in full view of all users.

Discharging wastewater
• Water drained from the cooling system can be discharged into the rainwater
system.

• The system must be designed to accommodate the discharge of boiling water.

• Water used to clean the vessel must be discharged into the wastewater system.

10
 Installation and Commissioning
Unpacking
For optimal operation, please follow the following instructions when unpacking your device.

4 persons are required to lift up the device and perform this installation.

Unpack the device in a clean and accessible area.

Keep the packaging in case the device needs to be moved.

Cut the pallet strapping.

Lift the cardboard sleeve and remove it.

Remove the front board of the pallet.

Unlock the front wheel.

Place a ramp that can support 150 kg on

the floor (180 kg for MEDIAWEL50).

Lift a little bit the unit and slide it onto

the ramp (4 persons using the side
handles to lift).

Use the side handles of the unit to slowly pull it down until reaching the floor.

Inventory of MEDIAWEL 30/50 parts and accessories

Part No. Quantity Name

N/A 1 USB DRIVE (user manual/QC/validation cycles)
MEDW2008 1 Magnetic stirrer
MEDW2032 1 Sampling tube
MEDW2034 1 Draining plug
MEDW4011 1 Sampling nozzle seals (x2)
MEDW2003 1 Sampling nozzle: Ø 12 mm
MEDW2004 1 Sampling nozzle: Ø 6.4 mm
MEDW4012 4m Inlet and outlet water tubing + 4 tubing clamps
MEDW3014 3 Decompression filters 0,22µ
MEDW3002 50 pcs. Carded cottons
MEDW4003V 1 Sliding washer stirrer axis
MEDW4008 1 V-ring seal on stirrer axis

11
Environment for Installing your MEDIAWEL

Only engineers trained by Alliance Bio Expertise can install the MEDIAWEL.

Your MEDIAWEL needs to be installed in a place which respects some

environmental conditions.
It must be installed indoor, and on a clean, dry, and flat floor.

Overall dimensions:

MEDIAWEL 30 MEDIAWEL 50

1053 mm

1083 mm

735 mm

585 mm 735 mm

585 mm

12
Site constraints
Atmosphere

o Operating temperature range: from 15°C to 30°C

o Maximum relative humidity: 80%.
o Non-condensing humidity.

Water supply

o Pressure: 2.5 bars minimum

o Temperature: 5°C to 20°C
o Hardness: <16°TH

The water hardness is the indicator for water mineralization. In other terms,
we are mentioning here, the scale level in the water. This scale can lead to
limestone solidification in the double jacket and lead to potential issues with
the MEDIAWEL. We therefore strongly recommend using an anti-limescale
device.
ALLIANCE BIO EXPERTISE accepts no liability for malfunctions or damage
caused by the presence of limescale inside the double jacket or other part of
the circuit.

Power supply

Alliance Bio Expertise shall not be held liable for any electrical issues if the
points below have not been complied with when switching the device on.
It is mandatory having an ELCB (electric line circuit breaker) with ground fault
protection according to country standard and currant noted below

Our equipment is intended to be connected to a strictly non-residential

electrical network in a commercial or industrial environment, and its design
or other features clearly exclude the possibility of residential use

Mediawel 30 (ref: MEDW1001) Mediawel 50 (ref: MEDW1050)

Voltage 400 V +/- 10 %, three-phase 400 V +/- 10 %, three-phase
Current 18 A max per phase 31 A max. per phase
Frequency 50/60 Hz 50/60 Hz
Power
9000 W +/- 10% 18700 W +/-10 %
consumption

13
 Mediawel 30 (ref: MEDW1002) Mediawel 50 (ref: MEDW1051)
208 V +/- 10 %, three-phase 208 V +/- 10 %, three-phase
Current 28 A max per phase 46 A max. per phase
Frequency 50/60 Hz 50/60 Hz
Power
7400 W +/- 10% 14500 W +/-10 %
consumption

First Commissioning

Connecting to the power supply

It is essential to fit the power cable with a plug that is suitable for the power required.
We recommend using the following kind of plug, which is especially suited to frequent
disconnection and reconnection operations:

GROUND GROUND
NEUTRAL

We recommend positioning the wall socket between 1 and 1.20 meters from the ground and
fitting it with a nearby cut-off device, in order to carry out connection and disconnection
operations with the power off.

14
Connecting to the water supply

>1 meter

1 meter

40 à 60 cm

o For more convenient use, include an easy to access tap close to the device, at a
height of approximately 1 meter and slightly offset at the side of the device as
illustrated below.
o Cut the 4 meter hose to the desired lengths for supply and outlet hoses
o Fit the in-line filter to the water supply hose (recommended option)
o Connect the supply and outlet hoses as set out below:

Water supply

Draining outlet
Water filter
3 1

Marking Name Part No.

1 In-line water circuit filter (recommended option) MEDW3008

2 and 3 Inlet and outlet water tubing MEDW4012

15
 Connecting to the water drainage

o The cooling water drainage system must be designed to withstand

a temperature of 110°C.

o It may be made from copper or PVC-C or any other material that is suitable for
this use.

o It must be equipped with a syphon as shown above.

Switching on and off the device

Prior to switching the device on, you must ensure that the mains voltage between phases,
and between phase and neutral, complies with the voltages required (as indicated in chapter
Site Constraints).

Filling the double jacket

Before running the first preparation cycle, it is essential to fill the double jacket with water.

You need to activate water circulation until you see water coming from the outlet hose
(2 – 4 minutes), using the WATER menu from the touchscreen. The water flow must be
between 4 and 6 liters per minute.

1. Select the CYCLE menu,

and press WATER.

2. Press ON to activate
water circulation.

3. Press OFF to stop it only when water is coming out from the drainpipe.

16
Presentation

Overview

10
2

9
4

8
5

1 Safety cover
2 Handle for opening the lid
3 Touch screen
4 Jacket pressure gauge
5 Vessel pressure gauge
6 Safety hatch (MEDIAWEL 30 only)
7 Front wheels with brake
8 USB port
9 Main switch
10 Lid

17
Rear view

1
4

1 Drain outlet

2 Water supply inlet

3 Decompression filter

4 Power cable

18
Lid details

1 Safety cover

2 Sampling port

3 Adding port

4 Vessel safety valve

5 Handle for unlocking the lid

6 Cover seal

7 Filter location (Bactystopper or disk filter)

19
Vessel inside

4
2

5
3

1 Vessel
2 Sampling tubing
3 Temperature probe
4 Stirrer
5 Monitoring probe port

20
Touch screen
The MEDIAWEL is fitted with a touchscreen that is composed of 4 parts:

A. Navigation in menus and submenus in stand-by mode.

B. Display of the temperature.
C. Information about the device and options’ status.
D. Information about ongoing actions, entry and cycle starting.

D
B

Under the CYCLE menu, you can choose:

• PROGRAM to start a cycle.
• STIRRER to define the stirrer speed.
• WATER to cool down manually the device.

Under the PARAMETERS menu, you can choose:

• PROGRAMS to edit and create dispensing programs.
• USERS to edit and create users.
• SETTINGS to set time, date and language.
• MAINTENANCE to access troubleshooting menu.

Under the ACCESS RIGHTS menu, you can decide of the access
rights for users.

Under the METROLOGY menu, you can calibrate the device’s

temperature probe.

21
Status Indicators

On both sides of the screen,

some color led lights indicate
the device status or alert the
user.

Indications are based on the rules appearing in the following table:

Colour Status

Clear Blue Standby

Blue Operating device

Flashing Orange Step alarm or an action is required

Red Major default. Please contact an authorized service engineer

Flashing Green End of cycle

22
Device Parameters

Before getting started with your MEDIAWEL, you need to set parameters such as the
operation language, or date and time.

Language / Date and time setting

Select PARAMETERS menu, and press Settings.

• To modify the date and/or

time, press the field to
enter the value(s).

• To modify the operating

language, use side arrow
keys to select the
language.

23
Managing Users

Creating a user

1. Select the
PARAMETERS menu,
and press USERS.

2. Use the arrow keys to

select an available user
number.

3. Press to create a new user.

4. Enter an operator
name.

5. When the Access rights

management is
activated (optional),
you need to define a
password and the
access rights assigned
to this new operator.

(Please refer to chapter “Activating the Access Rights Management”)

6. Press to confirm.

The unit is delivered with “access right” mode disable, in that state, no
password is needed to use the device. We recommend activating
“access right” only if you really need it.

24
Modifying a user

1. Select the
PARAMETERS menu,
and press USERS.

2. Use the arrow keys to

select the user to
modify.

3. Press to confirm.

4. Press Edit.

5. Modify settings and press to confirm.

25
Deleting a user

1. Select the
PARAMETERS menu,
and press USERS.

2. Use the arrow keys to

select the user to
modify.

3. Press to confirm.

4. Press Delete.

26
Activating the access rights management

Only the Admin user can access to this activation menu.

It is essential to first create this Admin profile that allows activating or

deactivating access right management (see chapter Creating a user).

When activating the access right management, you decide that users need to
log in before using the device (username + password).

Managing access rights means creating different user profiles with different
access rights.

The different profiles that can be created and their access rights are as
follows:

User Profile Access rights

Operator CYCLE menu and its submenus (Program, Stirrer, Water)

• CYCLE menu and its submenus (Program, Stirrer, Water)

Metrologist
• METROLOGY menu

Admin Full access

To activate the access rights management:

1. Select the ACCESS

RIGHTS menu.

2. Select the Admin

username from the
scrolling list.

3. Enter the admin

password.

4. Press to confirm.

27
5. Tick the Operator box if
you wish the users to
log in before using the
device.

When the access

right management
is activated, the
user is indicated.

OSCAR

You can log out by

clicking on this button.

The cycle’s end automatically logout the user

28
Managing programs

Creating a program

When creating a program, some predefined parameters are suggested. These

parameters are recommended for a standard use of your MEDIAWEL.

Cycle less than 5 Liters, (only on MEDIAWEL 30), a lower speed is recommended,
speed 2 before and during each step

Specific media may require different stirring speed.

1. Select the
PARAMETERS menu,
and press PROGRAMS.

2. Use the arrow keys to

select a program
available.

3. Press to create a
new program.

4. Press the field to enter

the program name.

5. Use the keyboard to

enter the program

name and press .

29
6. Once the program
name is displayed,

press .

7. Press the field to enter

the number of steps for
this new program.

8. Enter the number of

steps and press .

9. Press to confirm.

10. Enter the name for

step 1 and press

to confirm.

30
11. Enter the temperature
required for step 1 and

press to confirm.

12. Enter the duration

required for step 1 and

press to confirm.

13. If required, enable an

alarm and set a
message that can be
displayed. (Never
activate an alarm on
sterilization step) Press

to confirm.

14. Press the field to enter

the stirring speed to get
before the step, and

press to confirm.

31
15. Enter the name for

step 2 and press to

confirm.

16. Enter the temperature

required for step 2 and

press to confirm.

17. Enter the duration

required for step 2 and

press to confirm.

18. If required, enable an

alarm and set a
message that can be
displayed.

Press to confirm.

32
19. Press the field to enter
the stirring speed to get
before the step, and

press to confirm.

20. Press the field to enter

the stirring speed to get
before the step, and

press to confirm.

21. The new cycle settings

are summarized.

33
Modifying a program

1. Select the
PARAMETERS menu,
and press PROGRAMS.

2. Use the arrow keys to

select the program to
modify.

Press to confirm.

3. Press Edit.

4. Modify the program’s settings and press to confirm.

34
Deleting a program

1. Select the
PARAMETERS menu,
and press PROGRAMS.

2. Use the arrow keys to

select the program to
delete.

Press to confirm.

3. Press Delete.

4. Press to confirm.

35
 Running a Cycle

Preparation

Before effectively starting a cycle, some operations should be performed following some
good practices.

1. Check that the water supply and the drain hoses are properly connected.

2. Open the water tap.

3. Connect the power cable.

4. Switch the device on by pressing the master switch.

5. Open the lid and place a new

Bactystopper, or disk filter in the
housing.

Use Bactystopper if the

dispensing duration is less than 6
Hours

Use Disk filter if the dispensing

duration is more than 6 Hours

36
6. Secure the sampling tube into the draining plug.

7. Put the sliding washer through the sampling tube

and the draining plug.

8. Put the sampling tube assembly into the vessel.

9. Slide the stirrer through the sampling tube

assembly.

10. Insert the silicone tube into the sampling tube.

11. Pour in 1/3 of the total deionized water to be

prepared into the vessel.

37
12. Slowly pour the powder into the vessel.

13. Pour the remaining deionized water down the edge of the wall and on the
stirrer in order to remove the powder from the surfaces, to reach the total
volume to be prepared (maximum 30 liters for Mediawel 30 ; maximum 50 liters
for Mediawel 50).

14. Close the lid.

15. Close both caps.

16. Close the safety cover.

17. Close the drain access hatch

(only for MEDIAWEL 30).

38
 18. Make sure that indicators are blue.

Starting a Cycle

1. Select the CYCLE menu,

and press PROGRAM.

2. Use the arrow keys, to

select the program to
be started.

Press to confirm.

3. Use the arrow keys, to

select the operator
who’s in charge of
starting the cycle.

4. Press the
Batch number field.

39
5. Dial the media Batch
number, and press .

6. Press to confirm.

7. Enter the volume of

media to be prepared.

Press to confirm.

8. Follow instructions
displayed on screen.

Press to start the

cycle.

40
 9. Details about the
progressing cycle are
displayed.

When the cycle is over, you can start dispensing.

Dispensing

Before starting actual dispensing, you have to prepare your MEDIAWEL.

1. Press OK to start the dispensing step.

2. If required, move the device into the dispensing room.

Switch off the device. Unplug from the power Unplug the water quick
supply. connectors.

Connect the device back to Switch on the device.

the power supply.

41
3. Open the safety cover.

4. Pull the safety valve.

5. Remove the sampling port cap.

6. Sterilize the sampling port.

42
 7. Plug the sampling nozzle.

Interrupting a cycle

The cycle can be interrupted at any stage.

When interrupting a cycle, cold water immediately flows through the double jacket, if the
temperature of the medium was higher than 83°C

1. Simply press to
interrupt the cycle.

2. Click on OK to confirm.

3. Click on to confirm
the export of data to an
USB drive

43
 If no USB key is connected, a warning
message informs you.

You can connect one key and press

to start again data export.

the vessel should only be accessed once the vessel's safety valve has
been enabled (pull the ring upwards).
under no circumstances may the lid be handled without first opening
the safety cover
Make sure to wear protective gloves designed for high temperatures.
Surfaces may still be at very high temperatures and can cause burns.

44
 Cleaning

The cleaning process must be carried out in a suitable location, allowing movement around
the device.
You should have hot water supply nearby, a wastewater evacuation and an electrical
installation allowing the protocol to be carried out in complete safety

Handling very hot water requires long resistant gloves, protective goggles and/or a
visor, waterproof safety shoes and clothes covering the whole body and limbs. Clothes should
be waterproof to avoid thermal burns with synthetic fabric stuck to the skin.

Cleaning between cycles

1. Remove the dispensing hose and immediately rinse it

with hot water.

2. Open the lid.

3. Open the hatch and place a bucket beneath

the vessel (only on MEDIAWEL30) or position
the device over a floor drain.

45
 The hatch is only available on MEDIAWEL 30
If you have a floor drain, you can add an accessory.
to avoid any splashing.
MEDW2015 Vessel draining - connection kit
(Available on MED30 & 50)

4. Switch the device off.

5. Disable the system's circuit breaker. And

unplug the power cable.

6. Remove the stirrer.

7. Remove the sampling tube.

8. Take these components and place them in a

deep sink for immediate rinsing in hot water.

9. Wash the vessel in very hot water using a soft brush.

10. Wash the decanting and adding caps in hot water.

46
11. Remove the Bactystopper

Bactystopper must be replaced every cycle.

Disk filter every 10 cycles

12. Clean the seal with hot water.

13. Leave the lid opened until the next use.

47
 Maintenance and Servicing
The MEDIAWEL requires regular servicing to ensure good operation order.
Preventive maintenance must be carried out by trained personnel, authorized by ALLIANCE
BIO EXPERTISE.

Summary table
Frequency

360 720 1440

Element Operation 1 cycle 10 cycles
cycles cycles cycles
900 1800 3600
2,5 hours 25 hours
hours hours hours
Media preparator ✓ ✓
(Please refer to the Descaling if TH > if TH <
procedure below) 16° 16°
✓
Risk of
premature
Stirring system Cleaning
wear of
the sliding
washer
Inlet waterfilter (Option)
Cleaning ✓
(Ref MEDW3008)
Bactystopper
(Ref MEDW3003) Replacement ✓
Instead of inner disk filter
✓
Sampling nozzle o-ring Regular
Replacement
(Ref MEDW4011) check-up
necessary
Adding cap o-ring
Replacement ✓
(Ref MEDW4006)
Lid gasket
Replacement ✓
(Ref MEDW4005)
Inner lid disk filter
Instead of bactystopper Replacement ✓
(Ref MEDW3014)
Stirrer bearing
Replacement ✓
(Ref MEDW4007)
Sliding washer stirrer axis
Replacement ✓
(Ref MEDW4003V)
V-ring from bottom plug
Replacement ✓
(Ref MEDW4008)
O-ring from bottom plug
Replacement ✓
(Ref MEDW4009)
Stirrer belt.
Replacement ✓
(Ref MEDW4004)
Stirring motor
Replacement ✓
(Ref MEDW4060)

48
 Temperature probe adjustment

It is highly recommended to calibrate the device’s temperature probe once a year.

To perform the probe calibration & adjustment, please follow the steps below:
1. Fill the vessel with 30 / 50 liters of water.

2. Close the lid.

3. Remove the sampling cap from the lid.

4. Insert the reference temperature probe (a 42 cm

long probe with specific adapter) into the
sampling port.

5. Launch a cycle with a 2 step program: 121°C THERMOMETER WITH SPECIFIC

for 15 min, at speed 5 for the first step and PROBE ON REQUEST
42°C, at speed 5 for the second step.

6. Read the displayed temperature of the MEDIAWEL during the first step
(after 5 min of stability at 121°C) and read the one of the reference
thermometer at the same time. Write them down.

7. Read the displayed temperature of the MEDIAWEL during the second step
(after 5 min of stability at 42°C) and read the one of the reference
thermometer at the same time. Write them down.

8. Press to stop the cycle.

9. Select the METROLOGY menu (accessible only to users with Metrology

access rights)

10. In each field, enter the

measured values that you
wrote down.

11. Make sure the data are

correct and press

to confirm.

The temperature on the main screen will adjust according to the calibration
performed.

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 Descaling the device
The descaling has to be done every 6
months for a water hardness superior to
TH 16°.

Advised descaling solution: MEDW3012

(SOLD ONLY IN FRANCE – for other
destination, please use local a descaling
solution adapted to stainless steel and
brass application).

Required accessories:
• 1 meter + 1,5 meters of tubing with 12mm internal diameter
• 1 graduated bucket that can contain 3 liters minimum.
• 1 graduated bucket of 1 liter
• 1 Funnel on which tubing can be plugged.
• 2,5L of descaling solution suitable with brass and stainless steel
(ref: MEDW3012)
• Gloves and protection glasses

To perform the descaling, please follow the steps below:

1. Disconnect inlet and outlet tubing at the back of the MEDIAWEL.

2. Connect the 1 meter tubing

on the exhaust port and place
the free end inside the big
bucket.

3. Connect the 1,5 meter tubing

on the inlet port and connect
the funnel to the free end.

4. Switch on the MEDIAWEL and

place the red tubing higher than
the Vessel level (see figure
below)

5. Select water menu

6. Activate water circulation by

pressing the ON button.

7. Pour water into the funnel up to getting the tubing full of it

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8. Remove water from big bucket and put the tubing back inside.

9. Fill the funnel of descaling operation up to 2,5l of water in the big bucket.

10. Stop the water circulation by pressing STOP button.

11. Disconnect the inlet and exhaust tubing and put back the original one.

DO NOT OPEN THE WATER

TAP

12. Create a 2-step program (first step of 30 min at 95°C and second step at 75°C with
10 liters of demineralized water in the vessel).

13. Start the cycle without opening the water tap.

14. Wait for the second step temperature reaching (the time to reach the second step
is longer than usual because there is no water circulation).

15. Stop the cycle.

16. Open the water tap and use the MAINTENANCE menu to start a water circulation
to cool down the vessel.

17. Once the vessel is cooled down the procedure is ended.

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 Troubleshooting

On-screen messages and icons may inform you of a problem and/or the need for action.

Icons

• Communication between HMI and automaton cards normal

Failure of communication between HMI and automaton cards normal

SD card failure

Connected to an Ethernet network

USB key connected

Messages

Message Action
Follow the instruction of the message that have been
Step Start Alarm created for this message. Example: "Add blood"
The lid is not properly closed. Release the pressure from the
vessel by using the safety while the temperature is below
80°C. Then open the lid and close it again to get blue light
coming one. If not →Call service.
IT IS FORBIDEN TO HANDLE THE LID ABOVE 80°C
IT IS MANDATORY TO ACTIONATE THE SAFETY VALVE
Alarm 'Close Vessel Lid' BEFORE HANDLE THE LID
One of the safety lids (door trap or lid to access to the
dispensing port) is not closed or not properly closed-->
Close the lids
IT IS FORBIDEN TO HANDLE THE LID/ SAFETY COVERS
Alarm 'Close Safety Covers' ABOVE 80°C
Time to reach the sterilization set point is too long or the
Heating error ‘Abnormal temperature is not stable --> Make sure that the stirrer is
temperature variation’ running properly. If not, check the bearing state.

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 Time to reach the dispensing set point is too long or the
Cooling error ‘Abnormal temperature is not stable --> Make sure that the water tap is
temperature variation’ open.
Temperature warning ‘ The temperature was not in +/-2°C range during the
temperature is out of sterilization plateau- Make sure that temperature is within
tolerance’ the tolerance of your application before validating the cycle.
Just for information - Check the data records at the end of
Restart cycle after power on the cycle before validating the cycle.
'Media' temperature probe
error ‘Abnormal temperature
measurement’ The vessel temperature probe is defective --> Call service
Technical Error ‘Stirrer motor’ Defective stirrer --> Call service
Technical Error ‘Pressure
release solenoid’ Defective pressure release valve --> Call service
Technical Error ‘Cooling’ Defective cooling valve --> Call service
Technical Error ‘Locker
magnet’ Defective lid locking solenoid --> Call service
Communication Initialisation Switch off and on again the machine - Call service if it does
failed not solve the problem
Communication failed. Please
switch off and contact the Switch off and on again the machine - Call service if it does
after-sales service not solve the problem

53
 Technical Specifications

MEDIAWEL 30 MEDIAWEL50

Without packaging: Without packaging:

L585 * W1053 * H735 mm L585 * W1083 * H735 mm
Dimensions
With packaging: With packaging:
L850 * W900 * H1250 mm L850 * W900 * H1250 mm

Without packaging: 150kg Without packaging: 180kg

Weight
With packaging:170kg With packaging:210kg

Ambient temperature recommended from 15°C to 30°C

Maximum relative humidity recommended 80%, non-condensing

Atmospheric pressure required 3 bars minimum

Max. altitude Up to 2000 meters

Pressure: 3 bars minimum

Water supply Temperature: 5°C to 20°C
Hardness: <16°TH

208 V +/- 10 %, three-phase 208 V +/- 10 %, three-phase

Voltage
400 V +/- 10 %, three-phase 400 V +/- 10 %, three-phase

Current 18 A max per phase 28 A max. per phase

Fuse rating 208 V – 3 phases 32 A 50 A

Fuse rating 400 V – 3 phases 20 A 40 A

Frequency 50/60 Hz

Power consumption 208 V – 3 phases 7400 W +/- 10 % 14500 W +/- 10 %

Power consumption 400 V – 3 phases 9000W +/- 10% 18700 W +/-10 %

According to international standards EN61010-1 ED. 2011,

Electrical environment safety requirements for electrical equipment measuring,
control, and standards of use in laboratory.

Installation category Pollution category 2 environments

According to EN61010-1 ED. 2011, international standards

Electromagnetic compatibility and about the safety requirements for electrical equipment
performance for measurement, control, and laboratory use standards.
Requirements about the CEM (emission and immunity).

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“Empowered from our people’ skills and experiences, we, ALLIANCE BIO EXPERTISE, together with our
customers and partners decided to manufacture Microbiology automated equipment around essential values:
Customer Focus, Service, Loyalty, Innovations”

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 Contact us:
+33 (0)2 40 51 79 53
Email: [email protected]

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