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    Injection Lin Acc RM

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    Riad Manamanni
    The document describes a test of an injector's linearity and accuracy. The test injects varying volumes and creates a calibration curve of peak area vs injection volume. The test passes if the linearity R^2 is above 0.9990 and accuracy is within 0.2 ul. The results showed an R^2 of 0.9987 and accuracy of 0.1 ul, so the test passed.

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    Injection Lin Acc RM

    Uploaded by

    Riad Manamanni
    6 views3 pages
    The document describes a test of an injector's linearity and accuracy. The test injects varying volumes and creates a calibration curve of peak area vs injection volume. The test passes if the linearity R^2 is above 0.9990 and accuracy is within 0.2 ul. The results showed an R^2 of 0.9987 and accuracy of 0.1 ul, so the test passed.

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    The document describes a test of an injector's linearity and accuracy. The test injects varying volumes and creates a calibration curve of peak area vs injection volume. The test passes if the linearity R^2 is above 0.9990 and accuracy is within 0.2 ul. The results showed an R^2 of 0.9987 and accuracy of 0.1 ul, so the test passed.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    6 views3 pages

    Injection Lin Acc RM

    Uploaded by

    Riad Manamanni
    The document describes a test of an injector's linearity and accuracy. The test injects varying volumes and creates a calibration curve of peak area vs injection volume. The test passes if the linearity R^2 is above 0.9990 and accuracy is within 0.2 ul. The results showed an R^2 of 0.9987 and accuracy of 0.1 ul, so the test passed.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    INJECTOR LINEARITY & ACCURACY

    A C Q U I S I TI O N I N F O R M A TI O N
    System Name: BSM_SM_TQD Acquired By: sqte
    Project Name: SQT_E_BSM_SM_TQD_data Column serial Number:
    Sample Set Method: 030 Injection Lin Acc SSM Sample Kit 1 Expiration Date:
    Processing Method SMDO PM Sample Kit 1 Lot Number:
    Report Method Name: Injection Lin Acc RM Build Version: Empower 2 Software Build 2154 SPs
    User Name: System Sample Set Id: 2456 Result Set Id 3116
    Sample Set Name: Injection Lin Acc SSM Sample Set Start Date: 1/4/2011 9:48:40 PM EST

    The Injector Linearity and Accuracy qualification makes injections at varying volumes and creates a
    calibration curve of Peak Area vs. Injection Volume.

    The qualification passes if the Linearity R^2 value is > or = 0.9990 and the Accuracy absolute value of
    the X-intercept is < or = 0.2 uL.

    Calculation
    The Injector Accuracy is calculated using the following formula: |Y intercept/Slope|

    Sulfadimethoxine Inj ector Linearity & Accuracy Results Table

    Injector
    Linearity R^2 Linearity R^2 Injector Accuracy Inj ector
    Linearity R^2 Accuracy
    Specification Pass Fail Specification Accuracy
    Pass Fail
    1 R^2 > or = 0.990 0.9987 PASS < or = 0.2 ul 0.1 PASS

    Printed 8:13:42 PM US/Eastern 4/26/2011 Page: 1 of 3


    INJECTOR LINEARITY & ACCURACY

    A C Q U I S I TI O N I N F O R M A TI O N
    System Name: BSM_SM_TQD Acquired By: sqte
    Project Name: SQT_E_BSM_SM_TQD_data Column serial Number:
    Sample Set Method: 030 Injection Lin Acc SSM Sample Kit 1 Expiration Date:
    Processing Method SMDO PM Sample Kit 1 Lot Number:
    Report Method Name: Injection Lin Acc RM Build Version: Empower 2 Software Build 2154 SPs
    User Name: System Sample Set Id: 2456 Result Set Id 3116
    Sample Set Name: Injection Lin Acc SSM Sample Set Start Date: 1/4/2011 9:48:40 PM EST

    Ov erlay of Sulfadimethoxine Peak Chromatograms

    600000

    400000
    Intensity

    200000

    0
    0.60 0.80 1.00 1.20 1.40 1.60 1.80
    Minutes

    Inj ector Linearity Results Table

    Peak: Sulfadimethoxine
    Area
    Vial SampleName X Value Lev el Channel Id Result Id
    (µV*sec)
    1 2:F,1 Sulf adimethoxine 2 uL 2.0 465535 1 2481 3119
    2 2:F,1 Sulf adimethoxine 3 uL 3.0 702381 2 2490 3120
    3 2:F,1 Sulf adimethoxine 5 uL 5.0 1156575 3 2499 3121
    4 2:F,1 Sulf adimethoxine 6 uL 6.0 1432195 4 2508 3122

    Printed 8:13:42 PM US/Eastern 4/26/2011 Page: 2 of 3


    INJECTOR LINEARITY & ACCURACY

    A C Q U I S I TI O N I N F O R M A TI O N
    System Name: BSM_SM_TQD Acquired By: sqte
    Project Name: SQT_E_BSM_SM_TQD_data Column serial Number:
    Sample Set Method: 030 Injection Lin Acc SSM Sample Kit 1 Expiration Date:
    Processing Method SMDO PM Sample Kit 1 Lot Number:
    Report Method Name: Injection Lin Acc RM Build Version: Empower 2 Software Build 2154 SPs
    User Name: System Sample Set Id: 2456 Result Set Id 3116
    Sample Set Name: Injection Lin Acc SSM Sample Set Start Date: 1/4/2011 9:48:40 PM EST

    Inj e c tor Line a rity Re s ults

    Calibration Id 3117 Y Intercept -15834.3

    Equation Y = 2.39e+005 X - 1.58e+004 Slope 238751.4

    Fit Type Linear (1st Order) R^2 0.99875

    Sulfadimethoxine Inj ector Linearity Calibration Plot

    1.0x106
    Area

    5.0x105

    0.0
    0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00
    Injection Volumne (ul)
    R^2 0.99875

    Accuracy Calculation: Inj ector Accuracy = |Y intercept/Slope|

    Y intercept = -15834 Slope = 238751

    Inj ector Accuracy = | -15834 / 238751 |= 0.07

    Printed 8:13:42 PM US/Eastern 4/26/2011 Page: 3 of 3

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