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    Bulletin 3425 Ver A

    Uploaded by

    Igor de Oliveira
    The document provides a template for recording the steps and conditions of an electrophoresis and western blotting experiment. It includes sections for documenting the gel, transfer conditions, antibodies, washes, and detection used in the experiment.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Bulletin 3425 Ver A

    Uploaded by

    Igor de Oliveira
    13 views1 page
    The document provides a template for recording the steps and conditions of an electrophoresis and western blotting experiment. It includes sections for documenting the gel, transfer conditions, antibodies, washes, and detection used in the experiment.

    Original Title

    Bulletin_3425_Ver_A

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    The document provides a template for recording the steps and conditions of an electrophoresis and western blotting experiment. It includes sections for documenting the gel, transfer conditions, antibodies, washes, and detection used in the experiment.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    13 views1 page

    Bulletin 3425 Ver A

    Uploaded by

    Igor de Oliveira
    The document provides a template for recording the steps and conditions of an electrophoresis and western blotting experiment. It includes sections for documenting the gel, transfer conditions, antibodies, washes, and detection used in the experiment.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    LANES

    Electrophoresis Transfer Immunodetection


    11 15

    Electrophoresis and Western Blotting Layout 22 16


    EXPERIMENT

    USER DATE
    33 17
    MW STANDARD
    44 18
    GEL TYPE % BUFFER TIME VOLTAGE

    Remove the bottom sealing tape before you forget! 55 19


    MEMBRANE TYPE

    66 20
    TRANSFER CONDITIONS AMPS VOLTS TIME

    BLOCKING BUFFER TYPE VOLUME TIME


    77 21
    1º AB 1ST TARGET DILUTION TIME

    1º AB 2ND TARGET DILUTION TIME 88 22


    1º AB 3RD TARGET DILUTION TIME
    99 23
    1º AB WASH BUFFER VOLUME TIME CYCLES

    2º AB SPECIES AND CONJUGATE DILUTION TIME 10 24


    2º AB SPECIES AND CONJUGATE DILUTION TIME

    2º AB SPECIES AND CONJUGATE DILUTION TIME


    11 25
    2º AB WASH BUFFER VOLUME TIME CYCLES
    12 26
    ECL SUBSTRATE TYPE VOLUME

    Visit bio-rad.com/GelLayout
    NOTES 13 for more information.

    14
    BIO-RAD is a trademark of Bio-Rad Laboratories, Inc. © 2022 Bio-Rad Laboratories, Inc.
    Bulletin 3425 Ver A 22-0647 1022

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