A Review On Bovine Brucellosis: Epidemiology, Diagnosis and Control Options
A Review On Bovine Brucellosis: Epidemiology, Diagnosis and Control Options
A Review On Bovine Brucellosis: Epidemiology, Diagnosis and Control Options
Abstract: Brucellosis is economically important zonootic bacterial disease caused by genus Brucella. It
contains different species such as B. abortus, B.melitensis, B.suis, B.ovis, B.canis, B.neotome, B. microti that
affect terrestrial animals and B. ceti and B. pinnipedialis affect marine mammals. The first three species are
called classical Brucella. Three of them are differentiated into biovars. Brucella have no classic virulence
genes encoding capsules, plasmids, pili or exotoxins contributing to the persistence in the host and
multiplication within phagocytic cell. Brucellosis occurs worldwide, except a few countries that have been
successfully eradicated. The aborted fetus, fetal membrane and uterine discharges are considered as the major
source of infection. Brucellosis is mainly transmitted to animals by ingestion of contaminated feed and water,
by contact with infected aborted fetus, fetal membrane and genital discharges, and by artificial insemination
from infected bulls. The bacteria are preferentially localized mainly in the reproductive tract of pregnant
animals and consequently cause abortion (late abortion), retained fetal membrane and infertility, where as
orchitis and epididimitis are seen in males. Among the serological tests, RBPT for screening and CFT for
confirmatory are routinely used in Ethiopia. Brucellosis remains one of the most common zonootic diseases
worldwide with more than 50,000 human cases reported annually. It is mainly transmitted to humans through
the consumption of unpasteurized dairy products, direct contact with infected animal parts. The disease also
causes huge economic loses which arises from abortion culling of infected animal, hindering animal export
trades of a country, treatment costs, time and costs allotted for research and eradication programs.
Formulating effective control strategies are needed that includes surveillance to identify infected animals,
prevention of transmission to non infected animals and removal of the reservoir to eliminate the source of
infection. In addition, vaccination of susceptible animals is also important in areas where high prevalence of
brucellosis exists.
Keywords: Brucellosis, Control, Diagnosis, Prevalence, Transmission, Zonoosis
1. INTRODUCTION
Brucellosis is a highly contagious, zoonotic and economically important bacterial disease of animals
worldwide and it is considered by the Food and Agriculture Organisation (FAO), the World Health
Organisation (WHO) and the Office International des Epizooties (OIE) as one of the most
widespread zoonoses in the world (Schelling et al, 2003). The disease in cattle, usually caused by
Brucella abortus and occasionally by Brucella melitensis and Brucella suis, is characterised by late
term abortion, infertility and reduced milk production as a result of retained placenta and secondary
endometritis and excretion of the organisms in uterine discharges and milk. Full-term calves may die
soon after birth. In fully susceptible herds, abortion rates may vary from 30- 80% (Anonymous,
2007).
In Africa, bovine brucellosis was first recorded in Zimbabwe (1906), Kenya (1914) and in Orange
Free State of South Africa in the year 1915 (Chukuwu, 1985). However, still the epidemiology of the
disease in livestock and humans as well as appropriate preventive measures are not well understood
and such information is inadequate particularly in sub-Saharan Africa. The surveillance and control
of brucellosis in this region is rarely implemented outside South Africa (McDermot et al., 2002). In
dairy production, the disease is a major obstacle to the importation of high yielding breeds and
represents a significant constraint to the improvement of milk production through cross breeding
(Mustefa and Nicoletti, 1993).
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In Ethiopia, there is no documented information on how and when brucellosis was introduced and
established. Even though, several serological surveys have showed bovine brucellosis is an endemic
and widespread disease in urban, peri-urban, highland and lowland, extensive and intensive farming,
smallholder farms and ranches of the country (Dinka and Chala, 2009). However, most of the studies
on cattle brucellosis have been carried out in central and northern Ethiopia and do not provide an
adequate epidemiological picture of the disease in different agro-ecological zones and livestock
production systems of the country (Megersa et al., 2011).
The evidences of brucellosis in Ethiopian cattle have been serologically demonstrated by different
authors. A high seroprevalence of brucellosis (22%) has been reported in dairy herd of Cheffa state
farm (Sintaro, 1994), while most of the studies suggested a low seroprevalence (below 5%) in cattle
under crop-livestock mixed farming (Berhe et al., 2007; Ibrahim et al., 2010). There is a scarcity of
published literature on the status of cattle brucellosis in pastoral areas of the country where large
population of cattle are reared. So far, a study carried out in east Showa zone of Ethiopia showed a
relatively higher seroprevalence in pastoral than agro pastoral system (Dinka and Chala, 2009).
Generally, one of the highest priority diseases, both in sub-Saharan Africa and other regions of the
developing world is brucellosis. The importance of brucellosis reflects its widespread distribution
and its impacts on multiple animal species, including cattle, sheep, goats, pigs and humans. While
the importance of brucellosis is widely assumed, the benefits of programs to control it, relative to
their costs, need to be assessed. The important components of such an assessment are: (1) to
understand the epidemiology of brucellosis in different livestock production systems, (2) to evaluate
how different measures can reduce the transmission of brucellosis in these systems and (3) to
estimate the benefits to costs of different brucellosis control strategies and how these compare to
competing public-sector investments (Mc Dermot et al., 2002).
. Therefore, the objectives of this seminar are:
To review the epidemiology of the disease.
To highlight gaps in the current knowledge regarding the diagnostic and control
methods of brucellosis.
2. BOVINE BRUCELLOSIS
2. 1. Characteristics of Brucella
Brucella species are facultative intracellular, gram negative, non-spore-forming and non-capsulated,
partially acid-fast coccobacilli that lack capsules, endospores or native plasmids. They survive
freezing and thawing but most disinfectants active against gram-negative bacteria kill Brucella.
Pasturization effectively kills Brucella in milk. The bacterium is of 0.5-0.7µ in diameter and 0.6-
1.5µ in length. They are oxidase, catalase and urease positive. Although Brucella species are
described as non-motile, they carry all the genes except the chemotactic system necessary to
assemble a functional flagellum (Fretin et al., 2005). They belong to the alpha-2 subdivision of the
Proteobacteria, along with Ochrobactrum, Rhizobium, Rhodobacter, Agrobacterium, Bartonella, and
Rickettsia (Yanagi and Yamasato, 1993).
Ten genome sequences representing five species of Brucella (B. melitensis, B. suis, B. abortus, B.
ovis, and B. canis) are available and about 25 additional Brucella strains/species are being sequenced.
The genomes of the members of Brucella are very similar in size and gene make up (Sriranganathan
et al., 2009). Each species within the genus has an average genome size of approximately 3.29Mb
and consists of two circular chromosomes, Chromosome I, is approximately on average 2.11 Mb and
Chromosome II is approximately1.18 Mb. The G + C content of all Brucella genome is 57.2% for
Chromosome I and 57.3% for Chromosome II (Halling et al., 2005).
Based on a comparison of 10 published Brucella genomes, what is striking are the shared anomalous
regions found in both chromosomes consistent with horizontal gene transfer in spite of a
predominantly intracellular life style. The Brucella have no classic virulence genes encoding
capsules, plasmids, pili or exotoxins and compared to other bacterial pathogens relatively little is
known about the factors contributing to the persistence in the host and multiplication within
phagocytic cells. Also, many aspects of interaction between Brucella and its host remain unclear
(Seleem et al., 2008).
2. 2. Epidemology
2. 2. 1. Global distribution
The geographical distribution of brucellosis is constantly changing, with new foci emerging or re-
emerging. New foci of human brucellosis have emerged, particularly in central Asia, while the
situation in certain countries of the Middle East is rapidly worsening (Pappas et al., 2006).
The disease occurs worldwide, except countries which include Australia, Canada, Cyprus, Denmark,
Finland, Netherlands, New Zealand, Norway, Sweden and the United Kingdom which has
eradicated. This is defined as the absence of any reported cases for at least five years. However, the
Mediterranean Countries of Europe, Africa, Near East countries, India, Central Asia, Mexico,
Central and South America are still not brucellosis free. Although in most countries brucellosis is a
nationally notifiable disease and reportable to the local health authority, it is under reported and
official numbers constitute only a fraction of true incidence of the disease (Robinson, 2003).
High prevalence (24.5%) was reported from Sudan among African countries as indicated in Table 1.
In Ethiopia a high seroprevalence of brucellosis (22%) has been reported in dairy herd of Cheffa
state farm (Sintaro, 1994), while most of the studies suggested a low seroprevalence (below 5%) in
cattle as summarized in Table 2 below.
Table 1. Sero-prevalence of bovine brucellosis in different production systems in some African and Asian
countries.
S/No. Country No of Prevalence Test applied Authors and years Type of
cattle system
1 Zambia 1245 14.1 RBPT, c- Muma et a., l 2007 Extensive
ELISA
2 Kenya 393 1 c-ELISA Kang’ethe et al.,2007 Extensive &
Intensive.
3 Zimbabwe 1291 5.5 RBPT, c- Matope et al., 2010 Intensive
ELISA
4 Sudan 574 24.5 RBPT, c- Angara et al., 2010 Intensive
ELISA
5 Pakistan 200 3.0 MRT, ELISA Shafee et al., 2011 Intensive
Table 2. Seroprevalence of bovine brucellosis in Ethiopia in different geographical areas under different
production systems
S/No Study area No of cattle prevalence Type of Authors and Type of system
tested test years
1 Jimma zone 1,813 0.61 RBPT, Tolosa, 2004 Extensive & intensive
SAT
2 Tigray 1,951 1.49 RBPT, Berhe, 2007 Extensive & intensive
SAT
3 Bahr Dar 1,944 4.63 RBPT, Hailemelekot, Extensive & intensive
SAT 2005
4 Cent. 1,238 2.99 RBPT, Jegerfa, 2006 Extensive & intensive
Oromia SAT
5 AA 1,501 1.13 RBPT, Tefera, 2006 Extensive & intensive
&Suluta SAT
6 Tigray 1,968 4.9 RBPT,SAT Haileselassie, Semi-intensive &
2008 extensive
7 East Shewa 1,106 11.5 RBPT Dinka & Chala, Pastoral & agro-
2009 pastoral
8 Sidama 1,627 1.66 RBPT, Asmare et a., Extensive
zone SAT 2010
9 Jijjiga 435 1.38 RBPT, Degefu et al., Agro-pastorals
SAT 2011
10 South&East 1,623 3.5 RBPT, Megersa et al., Extensive
Eth SAT 2011
Survival of the first line of defense by the bacteria results in local infection and the escape of
Brucella from the lymph nodes in to the blood. During bacteraemic phase, bones, joints, eyes and
brain can be infected, but the bacteria are most frequently isolated from supra-mammary lymph
nodes, milk, iliac lymph nodes, spleen and uterus. In bulls, the predilection sites for infection are also
the reproductive organs and the associated lymph nodes. During the acute phase of infection, the
semen contains large number of Brucella but as the infection becomes chronic, the number of
Brucella excreted decreases. However, it may also continue to be excreted for years or just become
intermittent (Acha and Szyfres, 2001).
After the Brucella organisms spread through the hematogenous route in females then also reach the
placenta and finally to the fetus. The preferential localization to the reproductive tract of the pregnant
animal is due to the presence of the allantoic fluid factors that would stimulate the growth of
Brucella. Erythritol (four-carbon alcohol) is considered to be one of the factors, which are elevated in
the placenta and fetal fluid from about the fifth month of gestation. An initial localization within
erythrophagostic trophoblsates of the placentome adjacent to chorio-allntoic membrane results in
rupture of the cells and ulceration of the membrane. The damage to placental tissue together with
foetal infection and foetal stress inducing maternal hormonal changes may cause abortion (Radostits
et al., 2000).
2. 3. 2 Clinical signs
The primary clinical manifestation of brucellosis in animals is related to the reproductive tract. The
most obvious signs in pregnant animals are abortion (usually late abortion), birth of weak calves,
lowering of fertility with poor conception rates, retained fetal membrane, endometritis and reduced
milk yield. Orchitis and epididymitis are typical signs in males, and hygroma is usually common
during chronic infection (Corbel, 1997).
2. 4. Diagnosis and its Limitations
Clinician must develop a high degree of clinical suspicion based on epidemiological information and
history which are critical to making the clinical diagnosis. In all cases a blood sample should be
collected from the patient and laboratory testing should be requested as the definite diagnosis of
brucellosis is impossible without laboratory confirmation (Young, 1995). A proper and prompt
diagnosis is important as the treatment requires specific and prolonged antibiotics. Laboratory tools
include isolation and identification of Brucella from clinical samples, detection of antigen,
demonstration of genome and demonstration of Brucella specific antibodies (Solara et al., 1997).
2.4.1. Isolation and identification
Blood culture provides definite proof of brucellosis but may not provide a positive result for all
patients even under ideal conditions (Colmenero et al., 1997). Brucella are relatively slow growing
and the culture result may not become available for several days or weeks. They also need special
media with carboxyphlic environment. In particular for patients with chronic disease, the sensitivity
of culture can be low. Even though blood culture is the method of choice for isolation of the
causative agent, specimens need to be obtained early prior to antibiotic administration and need
prolonged periods of incubation. In addition, failure to detect the pathogen is a frequent
occurrence.The modern automated blood culture systems have somewhat improved the speed of
detection but are still too slow to make a rapid diagnosis (Bannatyne et al., 1997). Bone marrow
cultures are considered the golden standard for the diagnosis of brucellosis, since the relatively high
concentration of Brucella in the reticulo-endothelial system makes it easier to detect the organism.
Furthermore, bacterial elimination from the bone marrow is equivalent to microbial eradication.
However, in some studies results have not been universally reproducible, suggesting that the
bacteraemia is as unpredictable as clinical manifestations especially in human brucellosis (Shehabi et
al., 1990).
Identification of Brucella strains is done using standard classification tests, including Gram stain, a
modified Ziehl-Neelsen (ZN) stain, growth characteristics, oxidase activity, urease activity, H 2S
production (four days), dye tolerance such as basic fuchsin (1: 50000 and 1: 100000) and thionin
(1:25000, 1:50000 and 1:100000) and seroagglutination. It has been also recommended that Gram
stain morphology and modified ZN staining, coupled with the urease test, for rapid identification of
Brucella to the level of genus where facilities for further identification are not available. Laboratory
detection of Brucella and species identification is based largely on culture isolation and phenotypic
characterization. This process is lengthy and labour-intensive and has been associated with a
heightened risk of laboratory-acquired infections. To surmount these problems, nucleic acid
amplification has been explored for the rapid detection and confirmation (Mantur et al., 2006).
2. 4. 2. Antigen and genome detection
Enzyme linked immune sorbent assay (ELISA): Still now, there is only one report (Al-Shamahy
and Wright, 1998) suggesting antigen detection by enzyme-linked immunosorbent assay (ELISA) as
an acceptable alternative to blood culture for the diagnosis of brucellosis since sensitivity and
specificity are 100% and 99.2% respectively. Antigen detection methods are potentially useful but
have not been validated. Though co-agglutination has been reported as a technique for antigen
detection, there seems to be paucity of published literature.
Polymerase chain reaction (PCR): is fast and can be performed on any clinical specimen. A
number of nucleic acid sequences have been targeted for the development of Brucella genus-specific
PCR assays, including 16S rRNA, the 16S-23S intergenic spacer region, omp2 and bcsp31 (Navarro
et al., 2002) .Recently, Redkar et al. (2001) described real-time PCR assays for the detection of B.
abortus, B. melitensis and B. suis biovar1. These PCR assays target the specific integration of IS711
elements within the genome of the respective Brucella species or biovar. Currently, a real-time
multiplex PCR assay has been developed for rapid confirmatory identification of Brucella with
speciation. The genus, B. abortus and B. melitensis specific primers confirm the organism from
isolates. Although in the last few years, PCR is very promising and PCR-based laboratory tests have
been proposed, they cannot be considered a routine diagnostic method yet since standardization of
extraction methods, infrastructure, equipment and expertise are lacking and a better understanding of
the clinical significance of the results is still needed (Navarro et al., 2004).
Molecular characterization techniques are also very useful tools for differentiating Brucella spp.
especially follow-up testing of unusual phenotypic results of Brucella isolates. The use of molecular
methods in Brucella endemic areas needs to be explored before they can be applied in these areas to
diagnose brucellosis.
2. 4. 3. Serologic tests
The above limitations make serology for antibody detection the most useful tool for the laboratory
diagnosis of brucellosis. Antibodies usually begin to appear in the blood at the end of the first week
of the disease, IgM appearing first followed by IgG. The serological tests like Rose Bengal Plate
Agglutination Test (RBPT), standard tube agglutination test (SAT), Coombs test, immune capture
agglutination test, complement fixation test, ELISA, lateral flow assay-a simplified version of
ELISA, milk ring test (MRG) are commonly used tests in the diagnosis of brucellosis (Lucero et
al., 2003)..
Rose bengal plate test (RBPT): is often used as a rapid screening test. The sensitivity is very high
(>99%) but the specificity is disappointingly as low as 68.8% (Barrsol et al., 2002). However, this is
of value as a screening test in high risk rural areas where it is not always possible to perform the tube
agglutination titration test. To increase the specificity and the positive predictive value of the RBPT,
the test may be applied to a serial dilution (1:2 through 1:64) of the serum sample. The specificity of
the test increases when higher dilutions agglutinate and titers of 1:8 or 1:16 and above may be
regarded as positive. This approach may result in a lower sensitivity. Whenever possible, a serum
that gives a positive result should be confirmed by a more specific test. The RBPT is also of value in
the rapid confirmation of neurobrucellosis, arthritis, epididymo-orchitis, hydrocele due to Brucella if
the neat is positive in CSF, synovial fluid, testicular fluid /semen and hydrocele fluid respectively
(Manture et al., 2006).
Complement fixation test (CFT): detects specific antibodies of the IgM and IgG1 type that fix
complement. The CFT is highly specific but it is laborious and requires highly trained personnel as
well as suitable laboratory facilities that makes less suitable for use in developing countries.
Although its specify is very important for the control and eradication of brucellosis, it may test false
negative when antibodies of the IgG2 type hinder complement fixation. The CFT measures more
antibodies of the IgG1 than antibodies of the IgM type, as the latter are partially destroyed during
inactivation Since antibodies of the IgG1 type usually appear after antibodies of the IgM type,
control and surveillance for brucellosis is best done by CFT (Buchanan and Faber, 1980).
Serum aglutination test (SAT): developed by Wright and colleagues remains the most popular and
yet used worldwide diagnostic tool for the diagnosis of brucellosis because it is easy to perform, does
not need expensive equipments and training. SAT measures the total quantity of agglutinating
antibodies IgM and IgG (Young, 1991). The quantity of specific IgG is determined by treatment of
the serum with 0.05M 2-mercaptoethanol (2ME), which inactivates the agglutinability of IgM. SAT
titers above 1:160 are considered diagnostic in conjunction with a compatible clinical presentation.
However, in areas of endemic disease, using a titer of 1:320 as cut off may make the test more
specific. The differentiation in the type of antibody is also important, as IgG antibodies are
considered a better indicator of active infection than IgM and the rapid fall in the level of IgG
antibodies is said to be prognostic of successful therapy (Buchanan and Faber, 1980).
Drawbacks of the serum agglutination test include the inability to diagnose B. canis infections; the
appearance of cross-reactions of class M immunoglobulins with Francisella tularensis, Escherichia
coli O116 and O157, Salmonella urbana, Yersinia enterocolitica O:9, Vibrio cholerae, Xanthomonas
maltophilia, and Afipia clevelandensis; and the percentage of cases in which seroconversion does not
occur. Lack of seroconversion can be attributed to the performance of tests early in the course of
infection, the presence of blocking antibodies, or the so-called “prozone” phenomenon (i.e., the
inhibition of agglutination at low dilutions due to an excess of antibodies or to nonspecific serum
factors). Some of these shortcomings can be overcome by modifications such as the addition of
EDTA, 2-mercaptoethanol, or antihuman globulin (Young, 1991).
Indirect enzyme-linked immune sorbent assay (i-ELISA): typically uses cytoplasmic proteins as
antigens. ELISA measures IgM, IgG and IgA, which allows for a better interpretation of the clinical
situation. A comparison with the SAT, ELISA yields higher sensitivity and specificity. ELISA is also
reported to be the most sensitive test for the diagnosis of central nervous system brucellosis. Among
the newer serologic tests, the ELISA appears to be the most sensitive; however, more experience is
needed before it replaces the SAT as the test of choice for brucellosis (Almunneef and Memish,
2003).
The rapid and simple assays like Brucella IgM and IgG lateral flow and latex agglutination have
been developed recently. The sensitivity and specificity of lateral flow assay for culture confirmed
brucellosis is >95%. The sensitivity of the latex agglutination assay was determined to be 89.1% for
the initial serum samples collected for the patients with culture confirmed brucellosis and the
specificity was 98.2%. Both these tests are ideal for use as field tests in remote areas and as point of
care tests in hospitals and health care centers that lack the expertise and facilities to perform the more
demanding classic serologic tests (Abdoel and Smits, 2007).
Generally, all told, antibody profiles do not have specific clinical correlations, and titers often remain
high for a protracted period. The asymptomatic patient with an isolated positive titer of class G and
A immunoglobulins, or A immunoglobulin only, has not been adequately studied. Variations of
ELISA exist, such as competitive ELISA and sandwich ELISA, which may prove useful as a follow-
up tool (Ariza, 1992).
2. 5. Treatment
Due to the intracellular localization of Brucella and its ability to adapt to the environmental
conditions encountered in its replicative niche e.g. macrophage (Seleem et al., 2008), treatment of
domestic animals with antibiotics is not usually successful. Even though, treatment failure and
relapse rates are also high in humans, treatment depend on the drug combination of doxycycline with
streptomycin which is currently the best therapeutic option with less side effects and less relapses,
especially in cases of acute and localized forms of brucellosis (Seleem et al., 2009). Neither
streptomycin nor doxycycline alone can prevent multiplication of intracellular Brucella (Shasha et
al., 1994). Although the doxycycline-streptomycine regimen is considered as the golden standard
treatment, it is less practical because the streptomycin must be administered parentrally for 3 weeks.
A combination of doxycycline treatment (6 weeks duration) with parentrally administered
gentamicin (5 mg/kg) for 7 days is also considered an acceptable alternate regimen (Glynn and Lynn,
2008).
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Ashenafi Kiros et al.
cause 82,500 cases of brucellosis and 413 fatalities. Cases of laboratory-acquired brucellosis are the
perfect examples of airborne spreading of the disease (Ergonul, 2004).
Most common symptoms of brucellosis include undulant fever in which the temperature can vary
from 37C0 in the morning to 400C in the afternoon; night sweats with peculiar odor, chills and
weakness, insomnia, anorexia, headache, arthralgia, constipation, sexual impotence, nervousness and
depression (Acha, 2003). Human brucellosis is also known for complications and involvement of
internal organs and its symptoms can be very diverse depending on the site of infection and include
encephalitis, meningitis, spondylitis, arthritis, endocarditis, orchitis, and prostatitis. Spontaneous
abortions, mostly in the first and second trimesters of pregnancy, are seen in pregnant women
infected with Brucella (Khan et al., 2001).
Symptoms and signs of brucellosis usually referred as fever of unknown origin can be confused with
other diseases including enteric fever, malaria, rheumatic fever, tuberculosis, cholecystitis,
thrombophlebitis, fungal infection, autoimmune disease and tumors (Mantur et al., 2007).
Because of these rather non-specific signs, brucellosis is constantly mis-diagnosed as malaria, which
is very prevalent in sub Saharan Africa (Maichomo et al., 2009). Direct person-to-person spread of
brucellosis is extremely rare. Mothers who are breast-feeding may transmit the infection to their
infants and sexual transmission has also been reported (Kato et al., 2007).
3.2. Economic importance
Brucellosis causes heavy economic losses in livestock producers. The economic losses arises from
abortion, reduced milk production, losses of calves due to abortion and still birth, culling of infected
cows, hindering animal export trade of a nation, losses of man-hours, medical costs and government
costs incurred for research and eradication program (Georgios et al.,2005).
Although estimates of the costs associated with brucellosis infections remain limited to specific
countries, all data suggest that worldwide economic losses due to brucellosis are extensive not only
in animal production (culling infected animal, reduced milk, abortion and delayed conception), but
also in public health (cost of treatment and productivity loss). For example, official estimates put
annual losses due to bovine brucellosis in Latin America at approximately $600 million. Although
brucellosis eradication programs can be very expensive, they are estimated to save $7 for each $1
spent on eradication. The USA national brucellosis eradication program, while costing $3.5 billion
between 1934 and 1997, the cost of reduced milk production and abortion in 1952 alone was $400
million (Sriranganathan et al., 2009).
4. CONCLUSION AND RECOMMENDATIONS
Brucellosis remains one of the most common livestock and zoonotic diseases worldwide except in
those countries where bovine brucellosis has been eradicated. In developing countries brucellosis
appears to be more endemic especially in sub-Saharan countries including Ethiopia and its
prevalence is increasing due to sanitary, socio-economic and political factors. Existence of
brucellosis in a population is detected by identification and isolation on culture, serological tests and
PCR based molecular tests although each has limitations. Brucellosis is responsible for abortion,
retained fetal membrane, endometritis, orchitis, epidydimitis in animals and undulating fever in
humans. The worldwide economic losses due to brucellosis are extensive not only in animal
production but also in human health, but surveillance and control measures are not instituted
adequately.
Based on this literature review the following points are recommended:
Further nation-wide and integrated investigations in all production systems of different
geographical area should be conducted to have clear image on the magnitude and
distribution of the disease.
Herd and individual animal registration and compensation system should be practiced to
have good information, especially if test and slaughter policy is needed to be implemented.
Public awareness should be raised on the source of infection and method of transmission of
the disease to safeguard the public and for the planned control method to be effective
It is better to produce vaccine which give protection against all Brucella species and biovars
which cause bovine brucellosis.
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