Model 7400-25-33 Manual

Dear Valued Customer:
Thank you for purchasing CARON Products & Services equipment. We appreciate
your business and look forward to being your preferred supplier of controlled
environment equipment products in the future.
At CARON, we are committed to continuous quality improvement. Our goal is to

supply our customers with highly reliable equipment at a fair price. In order to openly
monitor our performance, we would appreciate your feedback on our products and
services.
If you have questions, or any suggestions for improvement based on the installation
or operation of the equipment you have purchased, please contact our service
department at www.caronproducts.com or 740-373-6809.
Thanks again for your business!
Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 2 of 110

Revision Log
Version Date Description

Rev A 10-2-14 Initial Release
Rev B 2-24-15 Added DLOG301 section
Rev F 09-07-16 Increased alarm delay for humidity from 15
minutes to 45 minutes
Rev G 10-03-16 Added information to the preventative
maintenance section
Rev H 08-14-17 Updated consistency between all manuals
Rev J 11-13-17 Added LTCH301/LTCH302 section
Rev K 02-13-18 Changed chamber drain tubing interface
Updated Co2 connection pic, added shaker
Rev L 03-08-18
specs limitations.
Rev M 04-03-18 Shaker info update
Rev N 05-17-18 HEPA filter info removed for CO2
Rev P 07-17-18 Updated PM Kit info
05-14-19 Added analog output adjustable temperature
Rev Q
range feature.
Rev R 11-05-19 Normalized all CO2 pressures to 20-25 PSIG
Rev S 1-20-20 Updated for flammable refrigerant
Rev T 1-21-21 Added adjustable alarm delay
Rev U 03-31-21 updated Declaration of Conformity document
Rev V 04-13-21 updated Declaration of Conformity document
Updated model numbers, removed DECN301
Rev W 05-07-21
option. Added thin pilaster features.
Rev X 07-01-21 Added CLEN301-304, WALL301-306 options
Updated pictures of Analog Outputs and Remote
Rev Y 11-03-21
Alarm Contacts
Rev Z 12-30-21 Added UKCA Declaration of Conformity

TABLE OF CONTENTS
Warranty .......................................................................................................... 6
Equipment Overview ..................................................................................... 10
Installation ..................................................................................................... 12
Unpacking
Choosing a Location
Preliminary Cleaning
Installing the Port Stopper
Installing the Shelves
Leveling the Unit
Connecting the Drain Line
Water Supply for Humidity
Connecting CO2 supply
Connecting Electrical Power
Cell Roller / Reinforced Shelf
Optional Accessory Installation ................................................................... 22
Connecting Alarm Contacts (ALRM302)
Installing Carboy water system (BOTL301)
Connecting to Humidity (HUMD304, HUMD307)
Connecting Analog Outputs (OUTP302, OUTP303)
Install a Drain Water Pump (PUMP301)
Install in a Wall - Trim Kits (WALL301/302)
Operation ....................................................................................................... 29
Using the Keypad
Learning the Screen Saver
Changing the Temperature Setpoint
Changing the Humidity Setpoint
Changing the CO2 Setpoint
Optional Accessory Operation ..................................................................... 38
Carboy water system (BOTL301)
Cleanroom Wipe down (CLEN301/302/303/304)
Operation of the Data Logging System (DLOG301)
Using the Extended Temperature Range (EXTD302)
Built in Gas Guard System (GASG302)
Ultraviolet Germicidal Lamp (LGHT602)
Interior Electrical Outlet (OUTL305-OUTL330)
Built in Temp or Temp/Rh 6” Pen Recorders (RCDR316/RCDR317)
Built in Temp or Temp/Rh 10” Thermal Recorders (RCDR318/RCDR319)
H2O2 sterilization cycle (STER305)
Door Latch (LTCH301/LTCH302)

Calibration...................................................................................................... 60
Calibrating Temperature
Calibrating Humidity
Calibrating the CO2
Alarms ........................................................................................................... 64
Alarm System Overview
Snoozing the Speaker
Muting the Speaker
Changing Alarm Setpoints and Delay
Alerts ............................................................................................................. 72
Alert System Overview
Maintenance
Info ............................................................................................................... 75
Info System Overview
Advanced Features ...................................................................................... 76

Setting the Time & Day
Door Heat
Humidity Control
Locking the controls
Changing the Passcode
Factory menu & troubleshooting
Preventative Maintenance ............................................................................ 92
Specifications ............................................................................................... 94
Electrical Schematics ................................................................................... 96
Troubleshooting .......................................................................................... 105
Spare Replacement Parts .......................................................................... 106
Appendix A – Declaration of Conformity ................................................... 109

WARRANTY INFORMATION
CO2 INCUBATOR LIMITED WARRANTY
Please review this section before requesting warranty service. At CARON, one of our primary goals is to
provide customers with high levels of personal service and top quality products, delivered on time, backed by
technical service and supported for the life of the product.
Before contacting us for warranty service, please be aware that there are repairs that are not covered
under warranty.
WARRANTY DEFINED
Caron Products & Services, Inc. (herein after CARON) hereby warrants that equipment manufactured by
CARON is free from defects in materials and workmanship when the equipment is used under normal operating
conditions in accordance with the instructions provided by CARON.
COVERED:
 Parts and labor for a period of two (2) years from date of shipment.
 Any part found defective will be either repaired or replaced at CARON's discretion, free of charge, by
CARON in Marietta, OH. Parts that are replaced will become the property of CARON.
 If CARON factory service personnel determine that the customer's unit requires further service, dependent
of the model involved, CARON may, at its sole discretion, provide a service technician to correct the
problem, or require the return of the equipment to the factory or authorized service depot.
 CARON will have the right to inspect the equipment and determine the repairs or replacement parts
necessary. The customer will be notified, within a reasonable time after inspection, of any costs incurred
that are not covered by this warranty prior to initiation of any such repairs.
NOT COVERED:
 Calibration of control parameters.
 Improper installation; including electrical service, gas and water supply tubing, gas supplies, room
ventilation, unit leveling, facility structural inadequacies or ambient conditions that are out of specification.
 Cost of express shipment of equipment or parts.
 Any customer modifications of this equipment, or any repairs undertaken without the prior written consent
of CARON, will render this limited warranty void.
 CARON is not responsible for consequential, incidental or special damages; whether shipping damage or
damages that may occur during transfer to the customer’s point of use. When the equipment is signed for
at the customer’s site, ownership is transferred to the customer. Any damage claims against the shipping
company become the responsibility of the customer.
 Repairs necessary because of the equipment being used under other than normal operating conditions or
for other than its intended use.
 Repair due to the customer's failure to follow normal maintenance instructions.
 Parts considered consumable; including: light bulbs, filters, gases, etc.
 Damage from use of improper water quality.
 Damage from chemicals or cleaning agents detrimental to equipment materials.
 Force Majeure or Acts of God.
This writing is a final and complete integration of the agreement between CARON and the customer. CARON
makes no other warranties, express or implied, of merchantability, fitness for a particular purpose or otherwise,
with respect to the goods sold under this agreement. This warranty cannot be altered unless CARON agrees
to an alteration in writing and expressly stated herein shall be recognized to vary or modify this contract.
Ohio Law governs this warranty.

EQUIPMENT INTERNATIONAL LIMITED WARRANTY
Please review this section before requesting warranty service. At CARON, one of our primary goals is to
provide customers with high levels of personal service and top quality products, delivered on time, backed by
technical service and supported for the life of the product.
Before contacting your distributor for warranty service, please be aware that there are repairs that are
not covered under warranty.
WARRANTY DEFINED
Caron Products & Services, Inc. (herein after CARON) hereby warrants that equipment manufactured by
CARON is free from defects in materials and workmanship when the equipment is used under normal operating
conditions in accordance with the instructions provided by CARON.
COVERED:
 Parts for a period of two (2) years from date of shipment.
 Any part found defective will be either repaired or replaced at CARON's or their authorized representative’s
discretion. Parts that are replaced will become the property of CARON.
 If CARON or their authorized representatives determine that the customer's unit requires further service,
CARON or the representative may, at its sole discretion, provide a service technician to correct the problem,
or require the return of the equipment to the an authorized service depot.
 CARON or their authorized representative will have the right to inspect the equipment and determine the
repairs or replacement parts necessary. The customer will be notified, within a reasonable time after
inspection, of any costs incurred that are not covered by this warranty prior to initiation of any such repairs.
NOT COVERED:
 Calibration of control parameters.
 Improper installation; including electrical service, gas and water supply tubing, gas supplies, room
ventilation, unit leveling, facility structural inadequacies or ambient conditions that are out of specification.
 Cost of express shipment of equipment or parts.
 Any customer modifications of this equipment, or any repairs undertaken without the prior written consent
of CARON, will render this limited warranty void.
 CARON and their representative are not responsible for consequential, incidental or special damages;
whether shipping damage or damages that may occur during transfer to the customer’s point of use. When
the equipment is signed for at the customer’s site, ownership is transferred to the customer. Any damage
claims against the shipping company become the responsibility of the customer.
 Repairs necessary because of the equipment being used under other than normal operating conditions or
for other than its intended use.
 Repair due to the customer's failure to follow normal maintenance instructions.
 Parts considered consumable; including: light bulbs, filters, gases, etc.
 Damage from use of improper water quality.
 Damage from chemicals or cleaning agents detrimental to equipment materials.
 Force Majeure or Acts of God.
This writing is a final and complete integration of the agreement between CARON and the customer. CARON
makes no other warranties, express or implied, of merchantability, fitness for a particular purpose or otherwise,
with respect to the goods sold under this agreement. This warranty cannot be altered unless CARON agrees
to an alteration in writing and expressly stated herein shall be recognized to vary or modify this contract.
Ohio Law governs this warranty.

Caron Products & Services, Inc.
PO Box 715 · Marietta, OH 45750
740-373-6809

INTERNATIONAL SYMBOLS AND DEFINITIONS
? Help
i Information
Warning of hazardous area
Warning of hot surface
Warning of dangerous electric voltage
Warning of risk of fire
Earth (ground) protective

conductor
WARNINGS
Local government may require proper disposal

TO LOCATE REFRIGERANT TYPE AND PRESSURES, SEE SERIAL
LABEL LOCATED ON THE OUTSIDE OF THE UNIT
FOR HYDROCARBON (R290 PROPANE) REFRIGERANT UNITS
R290 is highly flammable and must be treated with proper care.
Do not damage the refrigeration circuit. Do not store explosive

substances in the unit. Component parts shall be replaced with
like components and servicing shall be done by authorized
personnel to reduce the risk of possible ignition.
DANGER – Flammable Refrigerant Used. Risk of fire or

explosion.
 Do not puncture refrigerant tubing
 Do not use mechanical devices to defrost refrigeration
equipment
 Unit to be repaired only by trained service personnel
CAUTION – Flammable Refrigerant Used. Risk of fire or

explosion.
 Consult repair manual, owners guide before attempting to
service this product. All safety instructions must be
followed.
 Dispose of properly in accordance with federal or local
regulations.
CAUTION – Do not use any electrical appliance within the

Environmental Chamber, other than those recommended by the
manufacturer.

EQUIPMENT OVERVIEW
Congratulations! You have just purchased the latest technology in CO2 incubators.
Before using the equipment, familiarize yourself with key components of the product
and thoroughly read this manual.
7” Full
Replaceable air Color HMI
intake filter
Power
(refrigerated
Switch
models)
Right side
Heated Triple Pane CO2 Sample

Outer Glass Door Port
Right Side
Left Side Access Port
Access Port
RTD
Temperature
Sensor
Adjustable
Sliding Shelf Infrared CO2
(7400-25/33) Sensor
Humidity
Sensor
(Controlled
Humidity only)
Outer Door Drip

Trough
Model 7400-25
The following incubator models have pre-configured internal shelving and brackets
tailored for particular applications used in cell culturing:
7401-25/33 Multi-Tier Shaker Ready CO2 Incubators
CELL301/302 Cell Roller Ready CO2 Incubators
CELL303/304 Shaker / Cell Roller Ready CO2 Incubators

EQUIPMENT OVERVIEW – CONTINUED
Day / Time Actual Status Bar Actual

Temperature Humidity
Setpoint
Speaker Menu
Settings
Lockout Menu
icon
Parameter
Display
Zones
Current
screen
indicator
Navigation
Pane
(indicates
menu depth)
Submenu categories

INSTALLATION
Unpacking
Your new unit has been thoroughly packaged to avoid shipping damage. However,
the unit should be fully inspected upon arrival before signing for receipt. If the package
has visual damage, notes should be made on the freight bill and signed by the delivery
company. In the event of concealed damage after the unit is uncrated, keep the carton
and packaging material. Call the shipping company within 7 days of receipt, request
inspection and retain a copy of the inspection report.
For prolonged periods of inactivity leave the unit unplugged and securely stored.
Caron provides full on-site installation services for all models. Our installation
services guarantees the proper set-up and startup of all equipment. Please contact
the Service Department at 740-373-6809 or www.caronproducts.com
for details.
Choosing a Location
This product weighs in excess of 500 pounds.

Ensure that sufficient resources are available to
safely move the product.
To ensure proper operation, the unit must be located on a firm level surface, capable
of supporting approximately 800 pounds. The unit should be located in an 18°C – 25°C
ambient area and where there is no direct airflow from heating and cooling ducts as
well as out of direct sunlight. Allow four inches of clearance on all sides of the product
to allow for connections and airflow. The unit is designed to be used under the following
conditions.
 Indoor use only

 Altitude up to 2000m.
 Maximum relative humidity: non-condensing
 Mains supply voltage fluctuations up to +/- 10% of the nominal voltage;
damage may occur if voltage varies more than 10%
 Transient overvoltages up to the levels of overvoltage category II
 Temporary overvoltages occurring on the mains power supply
 Pollution degree: 2
 Ingress protection: IPXO
The unit requires a dedicated electrical connection. Depending on the options
purchased with unit a floor drain may be required. Choose a location where these
facilities are or can be made available. If a water source, or a drain is not available,
contact CARON customer service and ask about our CRYS102 product line or click
this web link for information on the product:
www.caronproducts.com

Preliminary Cleaning
Your new environmental chamber was thoroughly cleaned prior to leaving the factory.
It is recommended, however, to clean all interior surfaces with a general purpose
laboratory cleaning agent to remove any shipping dust or dirt prior to using the
product. Contact Caron if there is any doubt of the compatibility of the cleaning agent
being used with the chamber. After cleaning, dry all interior components with a sterile
cloth as necessary.
Installing the Port Stoppers
The unit has an access port built into each side of the cabinet. The ports are designed
to allow customer access for equipment validation or for installation of other equipment
inside the chamber. These ports should be sealed with the provided rubber stoppers
to allow the incubator to function properly. Install the stoppers provided in the port on
each side of the unit.
R290 REFRIGERANT UNITS
DANGER – Flammable Refrigerant Used. Risk of fire or

explosion.
 No equipment that uses an open flame should be placed
inside the unit.
 Do not use instrumentation or equipment that
incorporates potential ignition sources, e.g. open contact
switching, brushed DC and AC motors, etc.
 Do not use electrical appliances within the unit, other than
those recommended by the manufacturer.

Installing the Shelves
Models 7400-25 / 33 incubators include sliding perforated stainless steel shelves. Each
shelf requires two shelf channels for installation. Prior to installation, take time to
consider what the size of the product being placed in the incubator will be and set the
shelf spacing accordingly. Additional shelving can be purchased through CARON
customer service if necessary.
To install the shelf channels slide on end of the shelf track into the appropriate shelf
location in the rear plenum. Hook the front of the shelf track into the corresponding
location on either the right or left side front shelf support pilaster. Repeat the process
on the opposite side of the incubator.
Models 7401-25/33 have thin pilaster and reinforced shelving for larger shakers.

Shelf capacity: each perforated shelf is capable of supporting a
uniformly distributed load of 50 lbs, maximum chamber capacity is 500
pounds (stationary).
Standard shelving not suitable for shakers over the weight of 30lbs,
and have a .75” orbit.
Optional reinforced shelving is available for supporting heavy loads
such as shakers or cell rollers up to 150lbs.
Max shaker speed with reinforced shelves is 250 RPM.
Chamber should be empty when being moved.
Do not pull multiple loaded perforated shelves out simultaneously or

the chamber may tip.
Leveling the Unit
Place a level on the middle shelf of the incubator. Adjust the feet until the unit sits level
left to right and front to back. Even if the unit is level without adjustment, the leveling
feet should still be lowered to avoid the cabinet moving while opening and closing the
outer door & prevent a flat spot from forming on the casters.

Connecting the Drain
When using a pressurized water source, failure to connect

the unit to a drain could result in facility flooding.
The chamber drain connection is located in the bottom middle of the back of the
chamber. A 3/8” tube fitting, tubing and wire ties are supplied in the unit parts kit. Insert
the tube fitting into the tubing, secure tubing to fitting with provided wire tie. Insert fitting
into drain connection. Pull on the tubing after installation to make sure it is secure.
Route the drain tubing to a local floor drain. Duplicate fitting installation on other end of
tubing if necessary.
The drain line relies on gravity to remove water from the

chamber. The drain line must remain below the chamber
to drain properly. Kinks or elevations in the drain line
above the cabinet drain will not allow the chamber to drain.
If a local floor drain is not available, a variety of accessories are available through
CARON customer service. These accessories can also be viewed at
www.caronproducts.com
Models 7401-25/33 are non-humidified chambers and have a 1/2” plug installed into
the drain connection. The drain will be required when a humidity option is purchased
with the chamber.

Fill water pan (also HUMD305, HUMD306)
Use only distilled or deionized water with a

resistivity between 50K-CM and 1M-CM and
a pH of greater than 6.5. Using water outside
this range will void your warranty.
Do not use water that contains chloramines.

Chloramines can damage internal rubber
gaskets resulting in leaks.
A water pan is used to provide elevated humidity levels inside the incubator. This
feature is standard on 7400 models (and optional for the others). Using the handles
provided, remove the empty water pan from the bottom of the incubator. Fill with 5 to
6 liters of water. Re-insert water pan back into the incubator.
Connecting a CO2 supply
High concentrations of carbon dioxide can

cause asphyxiation. The use of CO2 monitors
and alarms is recommended for areas where
CO2 can collect.
The CO2 gas supply should be 99% pure and

should not contain a siphon tube. Gas pressure
to the unit must be regulated to 20-25 PSIG.
Failure to do so could cause tubing to burst.

The CO2 supply should be 99% and not have siphon tubes. CO2 pressure should be
regulated to 20-25 PSIG. CO2 tank regulators REGL101 can be purchased through
CARON customer service. Once the cylinder regulator is installed, connect the outlet
of the regulator to the hose barb fitting using the tubing and clamps provided.
An inline filter is provided to remove any contaminants in the CO2 gas supply. Check
the connections closely for leaks.
If the unit is equipped with a built in gas guard system GASG302, there will be 2 gas
inlets. Each of the inlets should be connected to an individual gas tank as described
above.

Connecting Electrical Power
Connect each incubator to a grounded circuit.

Failure to do so could result in electrical shock.
The unit requires a dedicated electrical outlet. See table below for model specific
power required and connection.
Model # Power Requirements Plug Connection

-1 115V, 60Hz, 16A FLA NEMA 5-20
-2 230V, 60Hz, 10A FLA NEMA 6-15
-3 230V, 50Hz, 8A FLA CEE 7/7
When the required electrical connection is available, plug the provided power cord
into the unit and the electrical outlet.
The mains power supply cord must meet the

requirements listed above. The use of an inadequate
mains power supply cord could result in equipment
failure or personal harm to the user.
In the event of a power outage the unit will

automatically restart when the power is restored.

Cell Roller Ready (CELL301,302,303,304)
These models have a cell roller, a reinforced floor, cell roller ramp, and interior outlet.
These models were designed to hold a 3, 5 or 7 tier Wheaton R2P cell roller.
Support braces have tracks to guide the cell roller wheels into the incubator. The cell
roller ramp has small tabs at the end that fit into an opening at the end of each cell
roller support brace.
The ramp is designed to support the weight of the cell roller as it is pushed into the
incubator. Once the cell roller is pushed into the incubator, remove the ramp and save
it in a convenient place should it need to be reused to remove the cell roller.
Cell Rollers can be heavy and awkward to

handle. Ensure that sufficient resources are
available to safely move the product.

OPTIONAL ACCESSORY INSTALLATION
Connecting Alarm Contacts (ALRM302)
With the purchase of ALRM302, a set of terminals on the rear of the unit is provided to
monitor temperature and humidity (controlled humidity option) alarms.
With the alarm contacts, the terminals provided allow for a NO (normally open) output,
a NC (normally closed) and COM (common) connection. In the event of an alarm
condition or power failure, the NO contact will close, and the NC contact will open.
Once the alarm is cleared, the contacts return to their normal conditions. Insert the
appropriate wire into the terminal and tighten down the screw terminal on top of the
connector.
Terminal Connection Unit off Normal Alarm

N/O to C Closed Open Closed
N/C to C Open Close Open
Alarm Contact
Connections

Installing Carboy water system (BOTL301)
Incubators can be purchased with an optional 2.5 gallon carboy water system. The
carboy system is preassembled and shipped inside the chamber. The four ¼” bolts
required to mount the carboy to the unit will be mounted in the left hand side of the
chamber. Remove the carboy assembly from inside the chamber and attach it to the
chamber using the ¼” bolts.
¼” Bolts
(4 total)
Tubing connects/
disconnect to
carboy
Attach the preassembled tubing provided with the carboy to the water inlet on the rear
of the chamber.
Tubing to water
inlet
Fill the carboy with water as described in the “Using the Carboy Water System” section
of the manual.

Connecting to Humidity System (HUMD304, HUMD307)
Use only distilled or deionized water with a

resistivity between 50K-CM and 1M-CM and
a pH of greater than 6.5. Using water outside
this range will void your warranty.
Do not use water that contains chloramines.

Chloramines can damage internal rubber
gaskets resulting in leaks.
If these water sources are not available contact CARON customer service.
A water inlet fitting on the back of the unit and ¼” black tubing are provided to connect
the water supply to the incubator. Connect an appropriate water supply to the fitting.
Incoming line pressure should be regulated to not exceed 80 psi.
CRSY102 or BOTL301 are accessories that can be used as water supplies. If a

Condensate Recirculator CRSY102 water recycling system was purchased as a water
supply, refer to its user’s manual for proper installation.

Connecting Analog Outputs (OUTP302, OUTP303)
With the purchase of OUTP302 or OUTP303, the controls are equipped with analog
outputs. OUTP302 provides 2 connections for monitoring temperature and humidity or
CO2. OUTP303 provides 3 connections for monitoring temperature, humidity and CO2.
Analog outputs provide either a milliamp (4-20mA) or voltage (0-5V) signal output to
represent each of the displayed temperature (and humidity) values. These options can
be used for connection to in-house data acquisition, recorder, or alarm system. The
temperature parameter (only) is adjustable in its scaling and is accessible at the Analog
Output screen.

Analog Output Current Corresponding Value
Parameter
Temperature 0–5V 4-20 mA -50 – 100 °C
(adjustable)
Humidity 0–5V 4-20 mA 0 – 100 %rh
CO2 0–5V 4-20 mA 0 – 20 %CO2
*Default range is -50C to +100C. Temperature scale low range is adjustable from -
50C to 0C. Temperature scale high range is adjustable from 1C to 100C.
Connect shielded wires to the appropriate signal terminals: I(+) for current (mA) or V(+)
for voltage (DC). For both current and voltage outputs, COM(-) is common terminal.

Installing Drain Water Pump (PUMP301)
Pump Outlet
to Sink or Floor
Drain
Pump Inlet
from Chamber
Drain
Reservoir with
Internal Level
Switch
In applications where a floor drain is not available and a CARON water recycling
system CRSY102 is not being used, a drain pump can be purchased to pump any
excess condensate from the chamber to a local sink or drain. The pump is located
near the middle of the back of the chamber. Connect the supplied tubing from the
pump to the sink / drain. The tubing may be run vertically into a ceiling but should not
exceed 15 feet height. The pump is equipped with a small reservoir on the bottom of
the pump with an internal level switch that will automatically turn the pump ON when
it is full to drain the water out of the reservoir and into a floor or sink drain.

Install in a Wall - Trim Kit (WALL301, WALL302)
Wall trim kits are intended to simplify and enhance installation in situations where the
incubator is embedded in a wall. In these situations, incubator sample content
access is from inside a cleanroom while incubator service access is from outside the
cleanroom. When used in conjunction with the corresponding WALL303/4/5/6 option,
the incubator itself is closed off front-to-back.
The trim kits consist of trim frame, gasket material, bottom skirt, and caulking. When
installed properly, the result is a double vapor barrier frame and coved floor skirt.
Installation does not require mechanical fasteners. See separate WALL301/302
Installation Instructions for details.
Model 7400-33-1 Model 7400-33-1

WALL302 Option WALL302 Option
(Painted Incubator) (Stainless Steel Incubator)

OPERATION
Before the incubator can be commissioned for use, make sure that the following steps
have been completed:
- Chamber is properly installed and level.

- The appropriate utilities connected to the chamber.
With the above mentioned steps complete, the power switch located on the right side,
near the top of the unit exterior, can be turned on.
Within a few minutes, the temperature and humidity will begin to approach setpoints.
Here is an overview of the home screen.
Status Bar Actual

Day / Time Actual Humidity
Temperature
Speaker Setpoint
Menu
Lockout Settings
icon Menu
Parameter
Display
Zones
Main screen with HUMD304, HUMD307 option

Using the Keypad
This control system uses a numeric keypad to enter all parameter values. Similar to a
calculator, this allows quick and precise entry of values. When any numeric value
button is pressed, the keypad display will pop up over the current display.
Range of variable Parameter Description

Header
Keypad
Display
Escape
Clear
Negative sign
Enter
Decimal
The Parameter Description Header tells what parameter is being changed.

The Keypad Display shows allowable values of the parameter being changed (initially)
and displays the entered value (when a button is pressed).
The Escape “Esc” button aborts the entry and returns to the previous screen without
changing the value. The Clear “Clr” button erases the value that you have entered.
After you have entered the value that you want, pressing the Enter “Ent” button and the
new value will take effect. This also closes the keypad window. Other keypad buttons
include a decimal point button and negative button.
If an invalid numeric button is pressed such that it would create an entry above the
parameter’s range, the entered number will not display. For example, if the
temperature setpoint range is 5.0 to 70.0, pressing ‘8’ followed by an ‘0’, only the ‘8’
will display.
If an invalid entry is made with an entry below the range (such as a ‘4’ followed by the
‘Ent’ button), then the entry will clear and the range will be re-displayed.

Learning the Touchscreen
To save power and ensure long product life, the touchscreen display has a few features
that can be changed to reduce screen brightness and initiate a Screen Saver mode.
High / Low button : high or low screen brightness, preset values.
Screen Saver : By pressing the Screen Saver button “on” this will
automatically enter screen saver mode after 15 minutes. At this time, the screen will
be completely blank (ie. black). The illuminated Caron logo (see Equipment Overview
section) shows that the unit is powered on and functioning. To wake-up the
touchscreen, simply press anywhere on the touchscreen and the main screen will
display. If the unit has an alarm condition, the touchscreen will not go into screen saver
mode. If an alarm condition occurs while in screen saver mode, the display will
automatically wake up and display the alarm.

Changing the Temperature Setpoint
The steps below walk through an example of changing the temperature setpoint from
37.0 °C to 20.0 °C. This example shows optional humidity control.
Here is the display of the home screen.
Actual Setpoint Button

Temperature
To set the temperature setpoint, press the (Setpoint) button on the right
side of the screen.
Temperature
Setpoint Button
Once the Setpoint screen appears, press the (Temperature Setpoint)

button. (In this example the temperature setpoint initially has a value of ’37.0’; this will
vary with different initial setpoint values.)

Keypad
A temperature setpoint window will appear. Enter the temperature setpoint by using
the keypad. For a setpoint of 20, press (‘2’), then (‘0’), followed by the
(Enter) key. Correct any mistakes with the (Clear button) and reenter
the value.
Once the Enter key has been pressed, the pop-up keypad disappears and the screen
returns to the Setpoint display with the new value of 20.0 in the temperature setpoint
button.
Temperature Home
Setpoint Button
Press the (Home) button to return to the main screen.

Changing the Humidity Setpoint (HUMD304, HUMD307 option)
Only controlled humidity will be displayed on the touchscreen, Evaporative pan

humidification will not be displayed.
The steps below walk through an example of changing the humidity setpoint. Here is
the display of the home screen.
Setpoint Button
Actual
Humidity
To set the humidity setpoint, press the (Setpoint) button on the right side
of the screen
Humidity Setpoint
Button
Once the setpoint screen appears, press the (Humidity Setpoint) button.
Keypad

Enter the new humidity setpoint on the keypad as desired and press (Enter)
when complete.
Home Button

Changing the CO2 Setpoint
If an alternative CO2 setpoint is required, the following steps can be taken:
Setpoint Button
Actual CO2
To set the CO2 setpoint, press the (Setpoint) button on the right side of
the screen
CO2 Setpoint
Button
Once the setpoint screen appears, press the (CO2 Setpoint) button.

Keypad
Enter the new CO2 setpoint on the keypad as desired and press (Enter) when
complete.
Home Button

OPTIONAL ACCESSORY OPERATION
Using the Carboy Water System (BOTL301)
To fill the carboy while attached to the incubator, unscrew the cap. Fill carboy with
distilled or deionized water (see Connecting the Water Supply section for details). The
carboy holds 2.5 liters.
If the carboy must be removed in order to fill it up, first disconnect the tubing between
the carboy and incubator by pressing the metal lever at the tubing connects /
disconnects at the bottom of the carboy. Then unscrew the four mounting screws and
remove the carboy. After re-attaching the carboy, connect the tubing by simply
pressing the plastic fittings into each other.
Screw Cap
Mounting Screws
(4 total)
Tubing Connects/
Tubing to Disconnect to
Incubator Carboy

Cleanroom Wipedown (CLEN301, CLEN302, CLEN303, CLEN304)
Caron offers upgrades to the base incubator model for applications where moderate
or aggressive chemicals are used to clean the incubator exterior. Bleach or chlorine
containing solutions should never be used to clean the incubator. The customer is
responsible to verify the chemicals used are compatible with the incubators. See
Caron’s Disinfectants Technical Bulletin for more details.
Incubators configured for moderate cleaning chemicals (CLEN301/302) have painted

exteriors, but have type 304 stainless steel hardware, hinges, & coated door strips.
Incubators configured for aggressive cleaning chemicals (CLEN303/304) have type

316 stainless steel exteriors as well as type 316 stainless steel hardware, hinges, and
coated door strips. Additionally, the touchscreen uses capacitive technology,
providing additional ingress protection.
Model 7400-33-1 Model 7400-33-1

CLEN302 Option CLEN304 Option
(Shown in-wall) (Shown in-wall)

Operation of the Data Logger (DLOG301)
The DLOG301 option provides the customer with a means of logging data electronically
for viewing at a later date. Logged variables are Temperature, Humidity, CO2 and Light
Intensity (but only if the chamber is equipped with those features.) All data is time-
stamped with year, month, day of the month, hour, minute, 24 hour time (ISO 8601
format). This data is stored internally in the chamber in non-volatile memory.
Note: The date and time are logged within the actual file name. The file’s “Date
modified” field is not maintained and therefore may not reflect the actual date
and time the file was created.
Data is logged every 5 minutes (provided the chamber is on); more than 10 years of
data can be stored in memory. If the internal memory fills up, new data overwrites the
oldest data.
Continuous writing to the flash drive necessitates a high quality

industrial grade device. Use only the flash drive provided by
Caron (or equivalent: single level cell memory, wear leveling
algorithms, error correcting code).
File name format is “DATE START YYYY-MM-DDTHH-MM_.csv” (hours in 24 hour

time)
When the chamber is on, the chamber’s history data is being stored even when a flash
drive is not inserted in the USB port. This data may be retrieved anytime using the
provided USB flash drive.

Here are the methods for retrieving data:
Continuous logging of data
Insert the flash drive into the chamber’s USB port. When first inserted, it creates a
.csv file called ‘DATA START’ with the current date and time in the file name. At 5
min intervals, the chamber’s process values are appended to the file. (The file will
get as large as the flash drive, permitting several years of uninterrupted data
storage.)
USB icon
USB icon appears in in Status bar indicating that data is being written to flash
drive.
To retrieve the data press the ‘Eject’ button, then insert the flash drive into a
computer to upload the data.
Upon re-insertion of the flash drive, a new .csv file is created, even if the old file
is still present. File name nomenclature is “DATE START YYYY-MM-DDTHH-
MM_.csv”.
History Retrieval
Auto Export
Select the ‘Auto Export’ feature on the USB menu screen. Insert the flash drive
into the chamber’s USB port. A new .csv file is automatically created on the flash
drive with all the stored history data. The file name nomenclature is “DATE END
YYYY-MM-DDTHH-MM_.csv”.
All Data
There is also an ‘All Data’ feature to indicate if the upload should include all data
(since the unit has been used) or just the history data since a flash drive was last
inserted. An ‘Info’ button will appear in the status bar warning the user not to
remove the flash drive while the data is being uploaded. The length of time to
upload the file will depend on the file size. When the ‘Info’ button disappears
from the status bar, press the ‘Eject’ button to safely remove the flash drive. Now
the data can be uploaded to a computer for viewing.
When using the Continuous Logging of Data method, nothing on the touchscreen has
to be setup. However using the History Retrieval method will require going into the USB
screen to select either the ‘Auto Export’ or ‘All Data’ buttons before inserting flash drive
into USB port.
To select the ‘Auto Export’ and ‘All Data’ buttons.
Settings
Button
Press the (Settings) button.

Setup Button
Press the (Setup) button.
USB Button
Press the (USB) button.
When the ‘Auto Export’ button is selected this will retrieve the data starting
at the point of the last download, and continuing to the present time.

When the ‘Auto Export’ button is selected this will retrieve the data starting
at the point of the last download, and continuing to the present time.
USB flash drive icon
Info button
Eject button
When flash drive is inserted into the USB port a ‘USB flash drive’ icon and flashing
‘Info’ button appears in the status bar indicating that the data is being downloaded to
the flash drive. Once ‘Info’ icon stops flashing select the ‘Eject’ button.
Wait until the USB icon disappears to safely remove the flash drive from the USB
port.
Note: Press the Eject button before removing the flash drive from the chamber,
otherwise there could be the risk of corrupt data.
Here is a graphic to illustrate how the data retrieval works.

Extended Temperature Range (EXTD302)
The extended temperature range is a factory installed option for model 7400. This
extends the temperature range down to 10°C. Simply enter a lower temperature
value when entering the temperature setpoint.
Built In Gas Guard System (GASG302)
An optional built in gas guard system is available to allow two tanks of CO2 to be
connected to an incubator requiring approximately 20-25 PSIG of gas pressure. The
unit is designed to automatically switch from the primary tank to the secondary tank
when low gas pressure of approximately 10 psig is detected on the primary tank. This
allows for a continuous supply of CO2 to an incubator after the primary tank is empty.
In addition, the user is notified of a tank empty scenario via an audible and visual alarm.
The CO2 gas supply should be 99.5% pure and

should not contain a siphon tube. Gas pressure
to the unit must be regulated to less than 30
PSIG. Failure to do so could cause tubing to
burst.
The CO2 gas supplies must be equipped with two stage regulators to ensure that the
incoming gas to the unit is regulated to appropriate levels. The high pressure stage
should have a 0-2000 PSIG range, and the low pressure gauge should adjust from 0-
30 PSIG. When connecting the gas supplies, adjust each tank output to 20-25 PSIG.
If the appropriate regulators are not available, contact CARON customer service to
purchase them.
Once the cylinder regulators are installed and adjusted on each tank, connect the outlet
of the regulator on Tank 1 to the hose barb fitting labeled Tank 1 on the back of the
unit. Repeat the process for Tank 2. Turn on the regulated gas supplies and check the
connections closely for leaks.

To access the internal Gas Guard,
Settings
Button
Gas Guard
Button
Press the (Gas Guard) button.
Press the (Tank 1 / Tank 2) button, will manually switch the tanks.

The factory default “master tank” is Tank 1. When the appropriate gas pressure is
supplied to both tanks, the master tank will always be used as the gas source. The
unit will swap from the master tank to the alternative tank whenever a low gas pressure
condition is detected.
Ultraviolet Germicidal Lamp (LGHT602)
Before removing access panel(s), disconnect

electrical power.
Avoid exposure to direct or reflected germicidal

ultraviolet rays. Germicidal ultraviolet rays are
harmful to the eyes and skin.
Replacing UV Light (optional accessory)

1. Turn off chamber and unplug power cord.
2. Remove the incubator top access panel. The housing is located in the back of
the incubator.
3. Unclip green wire with ground clip from UV light housing.
UV Light
Housing Cap
Green Wire with

Ground Clip
4. Pull UV light housing cap from UV light housing. Connected UV lamp will come
out with it.

See separate ultraviolet light owner’s manual for
specific warnings and instructions.
Follow local regulations for disposing lamps.
5. Discard used UV lamp

6. Insert new UV lamp into lamp connector socket.
7. Install UV light housing cap (with attached new UV lamp) into UV light housing.
8. Re-attach ground clip.
Ground clip must be securely attached to UV light

housing to reduce risk of electrical shock.
9. Install left access panel.

10. Plug power cord in and turn chamber on.

Interior Electrical Outlet (OUTL331 - OUTL340)
An optional interior electrical outlet is available to supply power to small interior

appliances such as shakers or stirrers. It is not intended to power high current draw
devices. Each outlet will handle up to 1.0 Amp. Incubators with a single interior outlet
will handle 1.0 amps total. Incubators with three outlets will provide 3.0 amps total. All
outlets are resettable GFI protected. Other outlet configurations can be purchased.
OUTL331/OUTL336 US outlet is 115V/60Hz

(Standard on model 7401,60Hz incubators)
OUTL332/OUTL337 European “Schuko” outlet

(Standard on model 7401, 50Hz incubators)
OUTL333/OUTL338 UK, British outlet
OUTL334/OUTL339 Australia outlet
OUTL335/OUTL340 Brazil outlet

Interior Electrical Outlet (OUTL321 thru OUTL330)
An optional interior electrical outlet is available to supply power to small interior

appliances such as shakers or stirrers. It is not intended to power high current draw
devices. Each outlet will handle up to 1.0 Amp if 115V or 0.5A if 230V. Incubators with
three outlets will hold 3.0 amps total if 115V or 1.5A total if 230V. All outlets are
resettable GFI protected.
This incubator is equipped with an internal hydrocarbon (HC) sensor. The status of the
HC sensor is indicated by the green/yellow/red indicator lights near the outlets.
LED Color Status Description Outlet

Green Sensor working and safe HC level Energized*
Yellow Sensor is initializing Not energized
Red Risky HC level detected Not energized
*If outlet is not energized, either the GFCI or fuse may have tripped.

DANGER – Flammable Refrigerant Used. Risk of fire or explosion.

 No equipment that uses an open flame should be placed
inside the refrigerator.
 Do not use instrumentation or equipment that incorporates
potential ignition sources, e.g. open contact switching,
brushed DC and AC motors, etc.
OUTL321/326 US outlet 115V, 60Hz
OUTL322/327 European “Schuko” outlet 230V, 50Hz
OUTL323/328 UK, British outlet is 230V, 50Hz
OUTL324/329 Australia outlet is 230V, 50Hz
OUTL325/330 Brazil outlet is 230V, 60Hz

Operation of Temp or Temp/Rh 6” Recorders (RCDR316/RCDR317)
Built in 6” ink pen temperature and or humidity recorders can be purchased with
CARON incubators. The recorders are shipped installed on the outer door of the
incubator from the factory and require no installation.
Changing the chart paper:
Chart paper Pen

cartridge
Recorder
control
buttons
Hub knob Pen arm
Backup
battery
Press and hold the “change chart” button on the recorder (#3) for approximately one
second until the pen begins to move to the left of the chart and then release the button.
Wait until the pen has completely moved off of the chart. To remove the chart paper,
unscrew (counter-clockwise) the chart “hub” knob at the center of the chart.

Remove the old chart paper and position the new one so that the correct line coincides
with the time line groove on the chart plate.
Re-attach the chart “hub” knob and fasten securely against the chart. Press and hold
the “change chart” button (#3) again for approximately one second until the pen begins
to move back onto the chart and then release the button. Check to make sure that the
pen is marking on the chart paper. If it is not, then carefully adjust the pen arm to
establish contact with the paper.
Chart recorder marking system:
This type of pen consists of a self contained ink reservoir with a porous plastic stylus
which is snapped around the outer edge of the metal pen arm. A pen cap is provided
to extend the life of the ink pen during shipping or when the recording unit is not in
service. To remove the pen cap, gently lift the pen arm away from the chart paper.
Remove the black plastic pen cap to expose the fiber tip of the ink pen and gently place
the pen back onto the chart paper. Do not let the pen arm "snap" back onto the chart
paper. This will flatten the fiber tip of the pen and will no longer give you a fine line
marking on the chart paper. Place the pen cap in a safe place for future use. If the
stylus does not touch the chart, adjustment can be made by slightly bending the metal
pen arm in the center towards the chart paper. Do not use more pressure than is
necessary to create a fine line marking on the chart paper. As the pen ink supply runs
out, the pen color will become lighter. This indicates that the pen should be replaced.
Replacement of the Pen:
Recorders that are equipped with fiber tipped cartridge pens will have a cartridge that
is color coded "red" to designate pen number one and an optional cartridge that is color
coded "blue" to designate pen number two. The pen cartridge is securely fastened
to the metal pen arm using a special "U" clip tab. For ease of replacement, it is
suggested that the two screws that hold the pen arm be loosened and the pen cartridge
and metal pen arm be removed as an assembly. Unsnap the plastic "U" clip tab of the
pen cartridge from the metal pen arm, remove and discard the old pen cartridge.
Replace the new cartridge by opening the hinge and snapping it securely around the
metal pen arm. Refer to the image below:

Pen Arm Calibration:
To check and/or adjust the recording pen(s) calibration to the outer most temperature
graduation of the chart, press and hold the "change chart" button (#3) until the pen
begins to move off of the chart. Once the pen(s) has moved off of the chart, again
press and hold the "change chart" button (#3) until the pen begins to move back onto
the chart. The pen should briefly stop at the outer most temperature graduation of the
chart before continuing onto the chart to begin recording. If the pen does not stop
exactly at this location on the chart, it can be adjusted or "calibrated" by using the left
(#1) or right (#2) arrow buttons.
When the pen moves back onto the chart and briefly stops, you will have approximately
five seconds in which to adjust the pen’s position using the left and right arrow buttons
of Figure 3.
On multiple pen recorders, each pen will move (one at-a-time) onto the chart briefly
stopping at the outer most temperature graduation of the chart at which time the pen’s
position can be adjusted by using the left (#1) or right (#2) arrow buttons. When the
time to adjust the position of the first pen has expired, the second pen will move onto
the chart briefly stopping at the outer most temperature graduation of the chart at which
time the second pen’s position may be adjusted.
Each time the chart paper or fiber tip pen cartridge is changed, you should make sure
that each pen stops at the outer most temperature graduation of the chart paper.
Otherwise, this pen offset will cause the unit to record an incorrect temperature on the
chart.
Recorder Calibration:
If a calibration adjustment is required for a single pen recorder, use the left (#1) and
right (#2) arrow push buttons on the recorder to calibrate (or move) the pen’s position
on the chart to correspond to the temperature of the solution. The arrow buttons
must be held for approximately five seconds before the pen will begin to move.
For two pen recorders, you must first select the pen that you wish to calibrate. This is
done by pressing the left (#1) arrow button to select the red pen or the right (#2)
arrow button to select the blue pen. The arrow button must be held down until the
green LED light goes out. After the green LED light goes out, follow the instructions in
step #3 above.

Battery Backup:
The green LED light remains a constant green color indicating that both the battery and
the main power to the unit are good. Refer to Figure 5 for the location of the green LED
indicating light. If the AC power were to fail or the battery becomes weak, then the
green LED light will begin "flashing" indicating that either you have lost the main power
to the unit or it is time to replace the battery. Having a 9 volt DC battery back-up in
place, will allow the recorder to continue to function normally for approximately 24 hours
in the event of a power failure.

Built In Temp or Temp/Rh 10” Thermal Recorders (RCDR318 / RCDR319)
Built in 10” thermal pen recorders can be purchased with CARON incubators. The
recorders are shipped installed on the outer door of the incubator from the factory and
require no further installation. Unlike ink pen recorders, the thermal recorders draw
their own chart and control lines.
The 10” recorders have been setup at the factory in the following configuration: 7 Day
/ 24 Hour / Temperature 0-100°C / Humidity 0-100% (for dual input recorders). If this
is not the ideal configuration for an application, the recorder may be reconfigured using
the following process:
Configuring the recorder:
In order to configure the recorder, you will need to enter the set-up mode of the
recorder. To enter the set-up mode of the recorder, press and hold the Change Chart
button (#3) until the thermal pen arm begins to move off scale and then release the
button.
Note: The green LED light will flash fast while the thermal pen arm is moving off scale.
Wait until the thermal pen arm has moved completely off scale and stops (the green
LED light will stop flashing and will be steady On). Unscrew (counter clockwise) the
chart "hub" knob at the center of the chart and remove the recording chart paper. Gently
lift the thermal pen arm just enough to be able to slide the paper out from beneath it.
Remove the recording chart paper and place the Setup Chart onto the recorder. This
chart contains the configuration categories of the recorder (Probe Input, Inner Chart
Temperature, Outer Chart Temperature, Temperature Scale, Chart Rotation Speed,
Input Filtering, Optional Relay Contacts and Date/Time for internal clock).
Next, press and hold either button #1 or #2 until the green LED light goes out and
release the button. If this step is successfully completed, the pen arm will move to the
outermost graduation ring of the Setup Chart. Use the Left (#1) or Right (#2) arrow
buttons to adjust the center of the thermal pen to be on this outermost graduation ring.
Position the Setup Chart so that the tip of the thermal pen is in the center of the Start
circle. Tighten the chart hub knob to secure the chart in place. Next, press and release
the Change Chart button to begin. The chart will rotate to the first category (Input #1).
Use the Left and Right arrow buttons to move the thermal pen arm to the desired option
of each category. Press and release the Change Chart button to accept the selection
and advance to the next category. You must press and release the Change Chart
button when you have finished configuring the last category in order to save all of the
changes that have been made to the recorder's configuration. The thermal pen arm

will move off of the chart allowing you to place the recording chart paper onto the
recorder. Press and release the Change Chart button to begin recording.
Changing the Chart Paper:
Press and hold the Change Chart button (#3) for approximately one (1) second until
the pen begins to move off scale and then release the button.
Note: The green LED light will flash fast while the thermal pen arm is moving off scale.
Wait until the thermal pen arm has moved completely off scale and stops (the green
LED light will stop flashing and will be steady On). To remove the chart paper, unscrew
(counter clockwise) the chart "hub" knob at the center of the chart. Gently lift the thermal
pen arm just enough to be able to slide the paper out from beneath it. Remove the old
recording chart paper and position a new one.
Re-attach the chart "hub" knob and screw securely (by hand) against the chart. Press
and hold the Change Chart button (#3) again for approximately one (1) second and the
thermal pen arm will move back onto the chart and begin recording.
Green Light LED Status:
The green LED light (located just below the three button membrane switch) is used to
show the recorder's status:
1.) LED on steady (not flashing) and input(s) recording within chart range, indicates
unit is recording normally.
2.) LED on steady (not flashing) and pen arm above outermost graduation and not
moving, indicates recorder is in Change Chart mode. Press and release Change Chart
button to return to normal recording mode.
3.) LED flashing rapidly and one or both inputs recording at outermost or innermost
graduation indicates a sensor break. Check or replace sensor(s). If sensor(s) are ok,
make sure process temperature is within configured range of recorder.
4.) LED flashing slowly (.8 seconds ON / .8 seconds OFF) indicates recorder is in
Set-Up mode. Refer to section CONFIGURING THE RECORDER.
5.) LED is Off indicates that there is no power to the recorder. Check A/C power to the
recorder.

Recorder Calibration:
If calibration is required for single input recorders, use the Left (#1) and Right (#2)
arrow buttons on the recorder to calibrate the temperature being recorded on the
chart to correspond to the temperature of the solution. The arrow buttons must be
held for approximately eight (8) seconds before the pen begins to move.
If calibration is required for dual input recorders, you must first select the input that
you wish to calibrate. This is done by pressing and holding the Left (#1) arrow button
to select Input #1 or the Right (#2) arrow button to select Input #2. The arrow button
must be held down until the green LED light turns off, after which follow the
instructions in single input instructions above.
Maximizing Pen Life:
In order to maximize the amount of life expected out of the thermal pen tip, follow
these simple rules:
1) Never let the thermal pen tip ride on the chart plate when the chart paper is not
present. This will damage the protective coating of the heating element.
2) Never use chart paper that is creased or that has been folded.
3) Periodically clean the thermal pen tip with a cotton swap dipped in alcohol. Clean
more often when operating the recorder in a dusty environment.
4) Always keep the door closed while the unit is recording.
5) Never lift the pen arm more than is necessary to remove and replace the chart
paper. Excessive lifting may cause a decrease in the pen tip pressure and cause
light printing.
Operation of H2O2 Sterilization cycle (STER305)
If the incubator is equipped with a STER305, then it is able to use the STER301
sterilization module (not included). STER305 includes an internal wiring connection
for the sterilization module as well as door safety interlock. See separate STER301
User’s Manual for details. Abide by all warnings.

Operation of the Door Latch with Optional Lock (LTCH301 / LTCH302)
The door latch is shipped installed on the incubator from the factory and requires no
installation. LTCH301 is the latch assembly only, and LTCH302 is the latch assembly
with an integral lock.
Door
Handle
Latch
Pull
Handle Lock
LTCH301 LTCH302
To operate the latch, grasp the pull handle and squeeze it toward the main door handle.
The latch will disengage. Continue pulling on the handle to open the door. Closing the
door fully will automatically re-engage the latch as long as the latch pull handle is not
squeezed. On LTCH302, inserting and turning the key when the door is closed will
activate the lock, prohibiting the pull handle from being squeezed enough to disengage
the latch.
Latch cannot be disengaged from inside the incubator

CALIBRATION
The temperature and humidity systems can all be calibrated as necessary. CARON
recommends an annual calibration check of each system. Before making a calibration
adjustment, allow the cabinet to stabilize a minimum of 12 hours from a power off
condition. If the unit has been in operation, allow a minimum of 3 hours of stable
operation at all setpoints.
If you do not have the appropriate reference instruments to perform calibration, contact
CARON’s service department for on-site calibration at www.caronproducts.com Caron
also provides validation services which ensure that the unit is functioning properly
according to IQ, OQ and PQ protocols which satisfy FDA guidelines for qualification
verification of equipment.
Be sure that all reference instruments are

calibrated to an appropriate standard.
The Calibration Screen
To get to the calibration screen from the home page:
Settings
Button
Main screen with HUMD304, HUMD307 option

Calibrate Button
Press the (Calibrate) button.
Temperature
Calibrate Button
Humidity
CO2 Calibrate Calibrate Button
Button
If optional features such as CO2 are purchased, a calibration button will also appear
for those options.

Calibrating Temperature
If temperature calibration is needed, the following steps can be taken:
Locate the reference instrument’s temperature sensor in close proximity to the

cabinet’s geometric center. Be sure that the stabilization times described earlier have
been satisfied prior to performing calibration.
Temperature
Calibrate Button
At the calibrate screen, press the (Temperature Calibrate) button.
Keypad
Enter the temperature offset by using the keypad and pressing (Enter) when
complete.
A positive value will move the temperature ‘up’ and a negative value ‘down’. Press the
‘home’ button and verify the proper temperature is displayed.

Temperature calibration (example)
If the chamber temperature display reads 40.0C and the calibrated independent
sensor shows 40.3C, set the temperature offset value to 0.3C. If the calibrated
independent sensor shows 39.6C, then the entered offset should be negative. In
this example the required offset to temperature would be -0.4C.
Calibrating Humidity
Only Controlled Humidity will be displayed on the touchscreen and can be calibrated.
If humidity calibration is needed, the following steps can be taken:

Locate the reference instrument’s humidity sensor in close proximity to the cabinet’s
geometric center. Be sure that the stabilization times described earlier have been
satisfied prior to performing this calibration.
A positive value will move the humidity ‘up’ and a negative value ‘down’. Press the
‘home’ button and verify the proper humidity is displayed.
Humidity calibration (example)
Only Controlled Humidity will be displayed on the touchscreen and can be calibrated
If the chamber humidity display reads 80% and the calibrated independent sensor
shows 83%, set the humidity offset value to 3.0%. If the calibrated independent
sensor shows 74%, then the entered offset should be negative. In this example the
required offset to humidity would be -6.0%.
Calibrating CO2
If CO2 calibration is needed, the following steps can be taken:

Locate the reference instrument’s CO2 sensor in close proximity to the cabinet’s
geometric center. Be sure that the stabilization times described earlier have been
satisfied prior to performing this calibration.
A positive value will move the humidity ‘up’ and a negative value ‘down’. Press the
‘home’ button and verify the proper humidity is displayed.
Calibrating Optional Chart Recorders
For calibrating the optional front and side mounted chart recorders, refer to section
(Optional Accessory Operation)

ALARMS
Alarm System Overview
The incubator control system is equipped with an alarm system that constantly monitors
temperature, CO2 and humidity (on controlled humidified models) to ensure the user is
notified if the cabinet goes into an alarm condition. Notification occurs via an alarm
pop-up window and a buzzer. Each alarm condition has been factory programmed to
minimize nuisance alarms while maximizing warning time. There is a 2 hour time delay
after start-up and setpoint changes. To avoid nuisance alarms after a routine door
opening, an alarm condition must be present for 15 minutes* (45 minutes for humidity)
before the operator is alerted. If the optional remote alarm contacts are present, in an
alarm condition, the dry contacts will change state.
*Alarm delays are adjustable, see “Changing Alarm Setpoints and Delay” for details.
The following alarm messages may be displayed:
 Chamber temperature is higher than setpoint temperature

 Chamber temperature is lower than setpoint temperature
 Chamber CO2 is higher than setpoint CO2
 Chamber CO2 is lower than setpoint CO2
 Door Open
 Temperature sensor error
In the event an alarm occurs, the alarm indicator will appear on the status bar and an
audible alarm pop-up window will automatically appear.
Audible speaker
Alarm icon
The flashing (Alarm) icon will appear on the status bar.

Alarm Screen
Alarm condition
icon
Alarm condition
Snooze Button
Close Window
Audible Alarm Snooze Function:
When in an alarm condition, the Audible Alarm can be temporarily silenced to avoid
being a nuisance to those nearby. The Audible Alarm will repeat after 1 hour has
passed, if the condition has not been corrected. (The audible alarm will not sound if
the alarm is muted, see Audible Alarm Mute)
Press the (Snooze) button, the audible alarm is silenced for a period of 60
minutes.
When the alarm condition is corrected the alarm indicator and the audible alarm will
automatically turn off (unless there is another alarm condition).
To check what the alarm condition is, press the (Alarm) button on the status bar.
and the alarm window will be displayed. If the (Snooze) button has already
been pushed and 60 minutes have not passed the Snooze button will be “greyed” out.
If you press the (Close Window) button, the Alarm Window will close, but
the alarm will still be present as a flashing alarm icon on the status bar for the
remainder of the 1 hour of time. It will not reset the 1 hour alarm countdown time if the
alarm condition is viewed on the pop up window.

After the 1 hour time has passed for an alarm condition, the counter will reset itself to
1 hour and repeat the countdown process again until the alarm has been resolved.

Audible Alarm Mute:
By factory default, when an alarm condition is present, the speaker will sound. This
speaker can be muted in an ‘on/off’ fashion eliminating all audible sounds. (Muting
the speaker will silence it until manually ‘un-muted’. This is different than ‘snooze’ in
the fact that snooze can only be enabled when an alarm condition is present and only
lasts for 1 hour.) When the speaker is muted, the alarm icon continues to flash and
the remote alarm contacts (optional) remain in the ‘alarm’ state.
To mute the audible alarm:
Settings
Button
Speaker icon
Speaker Mute
button
Press the (Speaker Mute) button.

Speaker Mute
icon Speaker Mute
button “On”,
indicator
The Speaker Mute button toggles to the “on” position ,and the speaker
icon changes to “ Speaker Muted” icon.

Changing Alarm Setpoints and Delay
All alarm setpoints were pre-set at the factory to minimize nuisance alarms that could
be created as a result of door openings. Alarm setpoints can be changed based on
individual user requirements. Alarm values are deviations from the setpoint and are
not actual setpoint values.
To change the alarm setpoints and delay:
Settings
Button
Alarms
Button
Press the (Alarms) button.
Temperature CO2 Alarm

Alarm Button Button
Humidity
Alarm Button

Alarm Delay
Press the (Temp Alarm) button.
Temperature
Alarm High Limit
Temperature
Alarm Low Limit
Once the alarm screen appears, press the (Temp High Limit) button.
Keypad screen will appear. Enter the High Temp Alarm value; press (Enter)
when complete.
To change the CO2 Alarm, press the (Alarms) button on the navigation
menu to go back to the Alarms screen. Press the (CO2 Alarm) and
(Humidity Alarm, Controlled Humidity only) buttons and repeat the same
steps for CO2 and humidity.

To change the alarm delay, press the alarm delay button and enter the
delay in minutes.

ALERTS
Alert System Overview
The chamber control system is equipped with an Alert system that constantly monitors
features of the chamber and notify the user if the cabinet needs any type of service to
ensure good running performance of the chamber. Alerts draw user attention to regular
maintenance needs, and minimize the risk of a future alarm condition.
When an Alert notification occurs, contact www.caronproducts.com with the serial

number of the chamber to order preventative maintenance kit(s).
Some of the Alert features are: Check the Atomizers (humidified units only), Replace
the Air Filter, and Check Equipment Calibration is Due.
Notification occurs via an Alert icon on the status bar. When the Alert icon is pressed,
a pop up window will display the alert condition(s). Each alert condition parameter is
factory pre-set, no adjustment is necessary.
Alert icon
Press the (Alert icon).
The Alert pop up window will appear displaying the alert message.

Press (Close Window) button to make the pop up window disappear.
Resetting Maintenance Alerts
Maintenance Menu Screen lets users check to see how much time is remaining on an
item that may need routine service or calibration. This is very convenient to inform the
user that a particular item will need to have service performed soon. After service has
been completed, the item needs reset and the alert will disappear.
Once the Settings screen appears, press the (Maintenance) button.

Reset button
Maintenance
items
Time left until

service is required
Once a Maintenance item is displayed on the Alert screen, it will continue to be present
as an icon in the Status Bar until the Maintenance item is corrected and the
(Reset) button is pressed resetting the replacement time to “new” status.

INFO
Info System Overview
The incubator control system is equipped with an Information system that constantly
monitors the incubator and to notify the user when an automatic condition is occurring.
This applies to conditions such as Decon Cycle or others that cannot be switched on
and off by the user but is controlled automatically by the software of the control system.
This notification cannot be disabled, it only lets the user know the incubator’s current
status.
Notification occurs via an Info pop-up icon on the status bar. When the Info icon is
pressed a pop up window will display the Info condition(s).
Info Button
The (Info) icon will appear on the status bar.

Info Screen
Press the (Close Window) button to return to the main screen.

ADVANCED FEATURES
Setting the Time & Day
The chamber has an internal real-time clock that keeps track of the day and time. It is
set at the factory to Eastern Standard Time and may need to be adjusted for your time
zone. To keep the clock accurate, it will need to be adjusted manually for daylight
savings time changes. To set the day & time:
Settings
Button
Setup
Button
Press the (Setup) button.
Day/Time Button

Press the (Day / Time) button.
Hour Button
AM / PM
Minute Button Button
Day of the
Week Button
Press the (Hour) button.
The Enter New Time in Hours window will appear. Enter the hour by using the keypad
and pressing (Enter) when complete.
Follow same procedure for setting up minutes.
To setup AM/ PM, Press (AM /PM) button and the words for AM and PM
will toggle back and forth.
To set the Day of the Week, press the (Day of the Week) button. This
button will scroll through the days of the week, press until the abbreviated letters
correspond to the actual day of the week.

Door Heat
Some Caron units are equipped with an integral door heater to keep internal
condensation off of the glass viewing area. This is only for units with humidity
control. In extreme ambient or running conditions, the factory default settings may
need adjusted to maintain a clear viewing area. Increasing the door heater
percentage will increase the amount of heat applied to prevent condensation. The
door heater can run in either automatic or manual mode. Automatic is the preferred
and default setting.
Door Heater
Button
From the Setup screen press the (Door Heater) button
Auto/ Manual
Button
Door Heat
Percent
Button
In Automatic mode, the door heater works in conjunction with the internal heaters to
maintain the temperature set point. In the event that the temperature is above set
point, the door heater will automatically throttle back.
In this mode, the output percent is a scale factor of the overall heat output
percentage. If condensation is present on the glass door under stable condition, then
increase the door heater percentage value. The factory default value is 10%.
To change the output percent value Press the (Door Heat Percent)
button.

Enter the hour by using the keypad, press the enter button when complete.
In Manual mode, the door heater is a fixed output value regardless of temperature set
point. This setting should only be used in units that have active cooling with
temperature set points well above the low-end range.
Warning: in manual mode, the temperature set point may not be maintained.
Humidity Control (models with controlled humidity)
This feature allows the Humidity to be controlled by a setpoint determined by the

user, which will be displayed on the home screen. If this feature is disabled, the
humidity value on the home screen will be in a “read only” condition. An icon in the
status bar will indicate whether the Humidity Control is enabled or disabled
Humidity Control
Button
From the Setup screen press the (Humidity Control) button

Humidity Control
disabled icon
Humidity Control
disabled indicator
When the humidity control is disabled the toggle button indicator is off and the
humidity control icon in the status bar has a red circle around it.
Humidity Control
enabled icon
Humidity Control
enabled indicator
When the humidity control is enabled the toggle button indicator is on and the
humidity control icon in the status bar has the red circle removed. Once the selection
has been made the status of the Humidity control will be visible on the home screen.

Locking the controls
To prevent unauthorized and accidental setpoint changes, the touchscreen can be

locked-out. The passcode is required to lock-out the controls and the same passcode
is used to unlock it. The factory default passcode is ‘1234’. This passcode can be
changed by the user to create a unique 4-digit passcode. There is also a feature that
will let you change the passcode from the factory default to a user defined passcode.
The factory default for the screen lock is “unlocked”
To lock the touchscreen,
Settings
Button
Control Lock
Icon “unlocked”
Password
Button
Press the (Password) button.

Press the (Lock Keypad) button.
The Enter the Current Passcode Keypad screen will appear.
Enter digits “1 2 3 4”; press (Enter) when complete.

The screen will change back to the Home Screen and the Control Lock icon will
change to the “locked” position.
Control Lock
Icon “locked”
When any button is pressed on the home screen the following pop-up window will
appear. If the button is pressed, the screen will change back to the Home
Screen.
To unlock the touchscreen,
From the previous Alert “Keypad is Locked” pop up screen, press the
(Unlock) button. The Enter New Passcode window will pop up.

Enter the digits “1 2 3 4”; press (Enter) when complete. The Control Lock Icon
will change back to the “unlocked” position.
Control Lock
Icon “unlocked”

Changing Passcode
To prevent unauthorized and accidental changes being made to the chamber, the
touchscreen can be locked-out. The passcode is required to lock-out the controls and
the same passcode is used to unlock it. The factory default passcode is ‘1234’. This
passcode can be changed by the user to create a unique 4-digit passcode. The current
passcode is required to change the passcode.
To lock the touchscreen,
Settings
Button
Control Lock
Icon “unlocked”
Password
Button
Press the (Password) button.

Press the (Change Passcode) button.
The Enter Current Passcode Keypad screen will appear.
Enter digits “1 2 3 4”; press (Enter) when complete.

The Enter New Passcode Keypad screen will appear.
Enter any new four-digit passcode (example: “2 5 8 0”). Then press (Enter)
when complete.
The Lockout screen will tell you that the Passcode has been changed to a new value.
This is only time that the Passcode will be displayed on the Lockout screen.

Factory menu & troubleshooting
The chamber control system is equipped with advanced diagnostics features which
allow the user to manually turn ‘on’ & ‘off’ each electronically controlled system. The
factory menu can be used to
 View the current chamber configuration
 See the percent output of the control system
 Manually and individually toggle any output
To access the Factory Menu,
Settings
Button
Factory
Button
Press the (Factory) button.

From the factory menu, four items can be selected. Press the (Model
Details) button to view the chamber’s configuration
Model Software
Number revisions
System Functions
& Options
From the Factory screen, press the (Output Percent) button to view the
current percent output level of each control parameter.

Output
1&2
Buttons
Navigating to the Output 1 or Output 2 screens

in the factory page will temporarily halt chamber
control & functionality.
To individually and manual control each output variable, from the factory screen press
the (Output 1) button. Note: Based on the chamber model number and
options, not all functions will be present.
Each item can be turned on to check the condition of that device or parameter to aid
in diagnosing a problem.

Press the (Output 2) button for other parameter buttons.
Chamber control & functionality is restored as soon as the screen is exited (Home,
Settings, or Factory buttons) .When finished with diagnosis in Output 1 or Output 2
screen, press the (Factory) button to return to that screen. Once you go
back to the Factory screen all parameters that were selected in Output 1 or Output 2
screens will reset to the “off” position.

PREVENTATIVE MAINTENANCE
Your CARON incubator has been robustly designed to minimize performance
problems. However, regular maintenance is very important for continuous trouble free
operation.
As a general rule, CARON recommends an annual calibration check of the
temperature, humidity, CO2 systems. CARON offers a full range of on-site calibration
and validation services. We also offer preventative maintenance contracts on our
equipment. Contact our service department for details at 740-373-6809 or visit us on
the web at www.caronproducts.com.
Recommended Daily Maintenance Checks
 Check the Temperature, Humidity, and CO2 displays versus setpoints.
 Check for and correct any alarm condition.
Recommended Monthly Maintenance Checks
 Check to ensure the drain in the bottom of the unit is draining properly.
 Check front air intake filter. If the filter is dirty replace it with Caron Preventative
Maintenance PM Kit. Washing the filter will result in poor performance.
Recommended Annual Maintenance Checks

 If humidity control option, replace atomizer nozzle (Replacement Parts section)
 Disinfect all interior surfaces with a general purpose laboratory cleaning agent.
 Perform a complete calibration of the temperature, humidity, and CO 2 systems.
 A full validation is recommended for GMP facilities each time a unit is installed, moved
or undergoes significant repair. Contact CARON’s service department to schedule
on-site validation.
 Replace UV lamp and clean quartz sleeve (feature optional)

Here is a list of PM Kits that are available for models and accessories covered in this
manual.
Model PM Kit Accessory PM Kit

1
7400-25 PM-7400-25 BOTL301 PM-BOTL301
DECN301 PM-DECN301
7400-33 PM-7400-33 3
EXTD302 PM-EXTD302
7401-25 PM-7401-25 FLTR301 3
PM-FLTR301
7401-33 PM-7401-33 HUMD304 3
PM-HUMD304
4
HUMD305 PM-HUMD305
5
HUMD306 PM-HUMD306
4,5
HUMD307 PM-HUMD307
2
LGHT602 PM-LGHT602
RCDR316 PM-RCDR316
RCDR317 PM-RCDR317
RCDR318 PM-RCDR318
RCDR319 PM-RCDR319
STER301 PM-STER301
1
only if humidity options
HUMD304, HUMD307 are installed
2
only if LGHT602 is installed
3
model 7400
4
model 7401
5
model CELL301/302/303/304

SPECIFICATIONS
MODEL 7400-25 7400-33 7401-25 7401-33
Temperature
10°C Above Ambient to 60°C 20°C to 60°C gROD
Range
Temperature
± 0.1°C
Control
Temperature
± 0.3°C
Uniformity
Temperature
RTD
Sensor
Humidity
Ambient to 95% RH none
Range
Humidity
± 3% RH* none
Control
Humidity
Capacitive none
Sensor
CO2 Range 0-20% CO2
CO2 Control ± 0.1% CO2
CO2 Sensor Infrared
32" W x 27" 32" W x 27" D
32" W x 27" D 32" W x 27" D
D x 52.7" H x 52.7" H
Interior x 66" H x 66" H
(81.3 cm x (81.3 cm x
Dimensions (81cm x 69cm (81cm x 69cm
68.6cm x 68.6cm x
x 168cm) x 168cm)
133.9cm) 133.9cm)
Interior
Type 304, 2B Finish, Solid Stainless Steel
Construction
35.5" W x
35.5" W x 33.3"
33.3" D* x 36" W x 33" 36" W x 33" D*
D* x 77.1" H
Exterior 77.1" H D* x 90" H x 90" H
(90.2cm x
Dimensions (90.2cm x (90cm x 85cm (90cm x 85cm
84.6cm x
84.6cm x x 229cm) x 229cm)
195.8cm)
195.8cm)
Exterior
Cold Rolled Steel, Powder Coated
Construction
25 Cu. Ft. 33 Cu. Ft. 25 Cu. Ft. (708 33 Cu. Ft. (934
Work Space
(708 Liters) (934 Liters) Liters) Liters)
# of Shelves Four (4) Five (5) 2 reinforced 3 reinforced
Type 304, Perforated

Shelf Type 304, Perforated Stainless
Stainless Steel,
Construction Steel, Electropolished
Electropolished
Shelf 29.25" W x 26.45" D 29.25" W x 26.45" D

Dimensions (74.3cm x 62.1cm) (74.3cm x 62.1cm)

Electrical
MODEL 7400-25 7400-33 7401-25 7401-33
-1 115V, 60Hz, 16A
-2 230V, 60Hz, 12A
-3 230V, 50Hz, 10A
Weight
MODEL 7400-25 7400-33 7401-25 7401-33
-1
525 lbs 600 lbs 525 lbs 600 lbs
(238 kg) (272 kg) (238 kg) (272 kg)
-2
825 lbs 900 lbs 875 lbs 1000 lbs

-3**
(374 kg) (408 kg) (397 kg) (454 kg)
Specifications are subject to change without notice.

Environmental Conditions: Temperature 15ºC to 25ºC, Humidity non-condensing
*Add 2.75 inches for handle
**Includes export shipping crate
*7400-25 Series units have forced internal air flow of 350 cfm (9,900 LPM)*
*7400-33 Series units have forced internal air flow of 450 cfm (13,000 LPM)*

ELECTRICAL SCHEMATICS
TROUBLESHOOTING
Problem -- Unit will not turn on
 Is the unit connected to a dedicated electrical circuit as defined in the installation section
of the manual?
 Is there power at the electric outlet the unit is plugged into?
 Is the unit’s power switch turned on?
Problem -- Unit temperature is above / below temperature setpoint

 Has the unit’s temperature setpoint been recently lowered / raised and if so has the unit
been allowed 12 hours stabilize at the new setpoint?
 Are the access port stoppers installed in the cabinet?
 Is the door closed?
 Is the condenser filter on the front of the cabinet clean?
Unit humidity level is above / below humidity setpoint

 Is the unit connected to a water source as specified in the installation section of the
manual?
 Has the unit been leveled to insure the cabinet drain works correctly?
 The cabinet’s drain line uses gravity to remove water. Does the drain line have any rises
in it above the cabinet’s drain level that could be trapping water?
 Has the unit’s humidity setpoint been recently lowered / raised and if so has the unit been
allowed time to stabilize at the new setpoint?
 Is the condenser filter on the front of the cabinet clean?
Unit CO2 level is above / below the CO2 setpoint

 Is the unit connected to a pressure regulated CO2 source as specified in the installation
section of the manual?
 Has the unit’s CO2 setpoint been recently lowered / raised and if so has the unit been
allowed time to stabilize at the new setpoint?
Internal outlet will not energize?

 Is LED sensor light green? Yellow indicates sensor is initializing. Red indicates a
problem.
 Has GFCI tripped?
 Is fuse blown?
 SPARE REPLACEMENT PARTS
CAUTION: Before servicing the unit, the mains power supply

cord must be unplugged to avoid risk of shock. Any area of the
unit that requires a tool to access shall only be serviced by
trained personnel approved by Caron Products.

Do not damage the refrigeration circuit. Component parts shall
be replaced with like components and servicing shall be done
by authorized personnel to reduce the risk of possible ignition.
General
Part Number Description

MTR-130 Blower Motor
BLW-112 Blower Wheel
CTR-140 Main Controller Board
CTR-141 CO2 Controller Board
CTR-142 Light Controller Board
CTR-144 7” Touchscreen, HMI
POW-115 24V DC Power Supply
FLTR301 Condenser Filter Replacement Kit
CRD-113 Power Line Cord
STP-101 2” Rubber Port Stopper
The mains power supply cord must be replaced by the

corresponding CRD part number above. The use of an
inadequate mains power supply cord could result in equipment
failure or personal harm to the user.
Temperature Related

HTR-166 Air Heater
RMT-117 107C Air Heater Thermostat
RTD-101 Temp Sensor -- RTD 100 Ohm Platinum
CMP-125 115V / 60Hz Compressor
SOL-108 Refrigeration Cooling Solenoid
Humidity Related

FIL-213 Air Purge Pump Filter
HUM-110 RH Sensor
PMP-150 24VDC RH Pressure Pump
NOZ-110 Precision RH Spray Nozzle
SOL-108 Dehumidification Solenoid
SOL-135 Humidification Solenoid
TUB-168 Drain Tubing, 3/8”
TUB-132 Water Supply Tubing, ¼”
CO2 Related

CO2-101 Carbon Dioxide Sensor
SOL-135 CO2 Injection Solenoid
FIL-213 In-line CO2 Filter
Fuse Related
ID Description 115V 230V

SW1 Main circuit breaker switch CBR-112 (16A) CBR-115 (10A)
FUS1 Heater fuse FUS-103 (10A) FUS-104 (5A)
FUS3* Internal outlet fuse (single duplex) FUS-151 (2A) FUS-151 (2A)
FUS3* Internal outlet fuse (double duplex) - FUS-163 (4A)
FUS5 Internal outlet transformer fuse - FUS-164 (3A)
* Fuse size varies depending upon whether the chamber has a single internal duplex outlet or two internal
duplex outlets
Options Related
Part Description Option

Number
CLM-132 Nylon tube clamp GASG301, REGL101
FIL-118 HEPA filter DECN301-1,DECN301-4
FIT-348 1/4”barb-1/4” push-in adapter GASG301, REGL101
MEM-103 USB Flash Drive DLOG301
PEN-103 Red pen for 6 inch recorder RCDR316, RCDR317
PEN-104 Blue pen for 6 inch recorder RCDR317
PPR-104 6 inch recorder paper, 7 day 0-60C RCDR316
PPR-105 6 inch recorder paper, 7 day 0-100C RCDR317
PPR-106 10 inch recorder thermal paper RCDR318, RCDR319
TUB-174 1/2” I.D. silicone tubing PUMP301
TUB-145 1/4” I.D. vinyl tubing GASG302, REGL101
TUB-174 1/2” I.D. silicone tubing PUMP301
WIR-102 20/3 conductor shielded wire ALRM302
EU DECLARATION OF CONFORMITY
Caron Products and Services, Inc.

27640 State Route 7
Marietta, OH 45750 USA
Declares that the product:
Designation: 7400 Series

Model Numbers: 7400-25-3, 7400-33-3, 7401-25-3-3, 7401-33-3
Classification: Electrical equipment intended for residential, commercial and lighting
industrial environments
Rated Voltage: 220-240 ~ (ac)
Rated Frequency: 50/60Hz
Meets the essential requirements of the following European Union Directive(s) using the relevant
section(s) of the normalized standards and related documents shown:
Directives: Low Voltage 2014/30/EU, EMC 2014/30/EU, RoHS 2011/65/EU

Standard: IEC 61010-1:2010
Safety requirements for electrical equipment for measurement, control, and laboratory use.
Part 1: General Requirements.
Standard: IEC 601010-2-012:2016

Safety requirements for electrical equipment for measurement, control, and laboratory use -
Part 2-012: Particular requirements for climatic and environmental testing and other
temperature conditioning equipment
Standard: EN 61326-1:2012
Electrical equipment for measurement, control and laboratory use - EMC requirements -
Part 1: General requirements
Signed for and on the behalf of Caron.
0
Date of issue: April 1,2021 in Marietta, OH, USA
By: Bob Dotterer

Engineering Manager, CARON
DECLARATION OF CONFORMITY
Caron Products and Services, Inc.

27640 State Route 7
Marietta, OH 45750 USA
Declares that the product:
Designation: 7400 Series

Model Numbers: 7400-25-3, 7400-33-3, 7401-25-3-3, 7401-33-3
Classification: Electrical equipment intended for residential, commercial and lighting
industrial environments
Rated Voltage: 220-240 ~ (ac)
Rated Frequency: 50/60Hz
Meets the essential requirements of the following UK legislation using the relevant section(s) of the UK
designated standards and related documents shown:
UK legislation: Electrical Equipment (Safety) 2016, EMC 2016, RoHS 2012

Standard: IEC 61010-1:2010
Safety requirements for electrical equipment for measurement, control, and laboratory use.
Part 1: General Requirements.
Standard: IEC 601010-2-012:2016

Safety requirements for electrical equipment for measurement, control, and laboratory use -
Part 2-012: Particular requirements for climatic and environmental testing and other
temperature conditioning equipment
Standard: EN 61326-1:2012
Electrical equipment for measurement, control and laboratory use - EMC requirements -
Part 1: General requirements
Signed for and on the behalf of Caron.
Date of issue: December 30, 2021 in Marietta, OH, USA
By: Bob Dotterer

Engineering Manager

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Dear Valued Customer:

At CARON, we are committed to continuous quality improvement. Our goal is to

Thanks again for your business!

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 2 of 110

Version Date Description

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 3 of 110

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 4 of 110

Advanced Features ...................................................................................... 76

Electrical Schematics ................................................................................... 96

Troubleshooting .......................................................................................... 105

Spare Replacement Parts .......................................................................... 106

Appendix A – Declaration of Conformity ................................................... 109

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 5 of 110

Ohio Law governs this warranty.

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 6 of 110

Ohio Law governs this warranty.

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 7 of 110

Warning of hazardous area

Warning of hot surface

Warning of dangerous electric voltage

Warning of risk of fire

Earth (ground) protective

Local government may require proper disposal

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 8 of 110

FOR HYDROCARBON (R290 PROPANE) REFRIGERANT UNITS

R290 is highly flammable and must be treated with proper care.

Do not damage the refrigeration circuit. Do not store explosive

DANGER – Flammable Refrigerant Used. Risk of fire or

CAUTION – Flammable Refrigerant Used. Risk of fire or

CAUTION – Do not use any electrical appliance within the

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 9 of 110

Heated Triple Pane CO2 Sample

Outer Door Drip

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 10 of 110

Day / Time Actual Status Bar Actual

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 11 of 110

This product weighs in excess of 500 pounds.

 Indoor use only

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 13 of 110

Installing the Port Stoppers

R290 REFRIGERANT UNITS

DANGER – Flammable Refrigerant Used. Risk of fire or

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 14 of 110

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 15 of 110

Chamber should be empty when being moved.

Do not pull multiple loaded perforated shelves out simultaneously or

Leveling the Unit

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 16 of 110

When using a pressurized water source, failure to connect

The drain line relies on gravity to remove water from the

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 17 of 110

Use only distilled or deionized water with a

Do not use water that contains chloramines.

Connecting a CO2 supply

High concentrations of carbon dioxide can

The CO2 gas supply should be 99% pure and

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 18 of 110

Model 7400-25-33 Series Operations Manual Rev Z 12-30-21 Page 19 of 110

Connect each incubator to a grounded circuit.

Model # Power Requirements Plug Connection