Nephchem Anti Streptosylin `O’

(Nephelometry method)
KIT NAME KIT SIZE CAT. NO
Nephchem - ASO 25 Tests NASO01025T GENUINE BIOSYSTEM

INTRODUCTION Step 1: Insert Test Card to Card reader slot and display will show
Anti Streptosylin O (ASO) is intended for Invitro quantitative promptly add R1 + S (sample)
determination of Anti streptosylin O in human serum. Anti-streptolysin O
(ASO) is the antibody produced in response to streptolysin O, an antigen Step 2: Pipette out 240 μl of R1 into dedicated cuvette and
produced by Lancefild group A streptococci. The World Health add 5 μl of sample (serum) and place the cuvette in the
Organisation recommends the use of ASO to aid the diagnosis of reading chamber
streptococcal infections. ASO titers are elevated in the sera 80 to 85% of
patients with rheumatic fever and in 95% of patients with acute Step 3: After the incubation, the display will show promptly add R2
glomerulonephritis. Raised ASO levels can also occur in other conditions
such as scarlet fever, acute rheumatic arthritis, tonsillitis and various Step 4: Pipette out 60 μl of R2 using sensor pipette with machine
other streptococcal infections. into the cuvette

METHOD PRINCIPLE Step 5: Once the reaction time got over, the result will show in the
The kit utilizes latex-enhanced immunoturbidimetry to measure the ASO display and (if external printer connected then it will get
level in human serum by GB NEPHCHEM (Nephelometry method) print out)
During the test, ASO in the sample binds with the Streptolysin O that is
coated on latex particles to cause agglutination. The turbidity caused by REFERENCE VALUES
agglutination is detected optically by GB NEPHCHEM, analyzer. The
change in absorbance is proportional to the level of ASO in the sample. upto 200.0 IU/ml
The actual concentration is obtained by comparing with a calibration
curve with known concentrations.
It is recommended for each laboratory to establish its own reference
KIT CONTENTS ranges for local population.

Reagent kit - box QUALITY CONTROL

To ensure adequate quality control, each kit can be cross checked with
R1 - ASO Buffer 1x 6.5 ml commercially available third party Immunological quality control or use
R2 - ASO Latex 1 x 1.6 ml recommended GB Immunology Quality control.
Test Card 1 no PERFORMANCE CHARACTERISTICS
Accessories kit box
Cuvettes 25 nos
• Linearity: 20.0 to 800 IU/ml
• Precision: within Run CV < 6 %
Big tips 25 nos • Specificity / Interferences
small tips 50 nos No interference detected for bilirubin upto 60 mg/dl,
hemoglobin upto 1000mg/dl, triglycerides 1000 mg/dl
Working reagent preparation and stability WASTE MANAGEMENT
Reagent R1 and R2 are ready to use liquid stable at 2-8°C till the expiry Please refer to local legal requirements.
date printed on the package.
LITERATURE
Concentrations in the test 1. Ayoub, EM, Harden E. Immune response to streptococcal
R1 - Buffer solution, sodium azide < 0.1% antigens: diagnostics methods. Manual of Clinical Laboratory
R2 - latex particles coated with anti-ASO, sodium azide < 0.1% Immunology, 6th ed. Rose NR, et al, eds.; 2002.
2. Cunningham, MW. Pathogenesis of Group A Streptococcal
Warnings and notes Infections, Clin. Microbiol. Rev. 13, 470-511; 2000.
1 The Kit is for in vitro diagnostic use only. Not for use in humans 3. Jacobs, DS, et al. Laboratory Test Handbook, 3rd Edition, Lexi-
or animals. Comp, Hudson (Cleveland); 1994.
2. The instructions must be followed to obtain accurate results. 4. Kaplan, EL, Rothermel, CD, Johnson, DR. Antistreptolysin O and
3. Do not use the reagents beyond the expiration date. Anti-Deoxyribonuclease B titers: Normal values for children ages
4. Treat all specimens as infectious. Proper handling and disposal 2 to 12 in the United States. Pediatrics; 101: 86-88, 1998.
procedures of specimens and test materials should be strictly 5. Klein GC, Baker, CN, Jones, WL. Upper limits of normal Anti-
followed. Streptolysin O and Antideoxyribonuclease B tiers. Applied
5. Reagents contain less than 0.1% sodium azide as preservative; Microbiology, 1971; 21: 999-1001; 1971.
avoid contact with skin and eyes, flush with copious amounts of 6. Krmakar, MG, Venugopal, V, Joshi, L., Kamboj, R. Evaluation &
water when disposing. revaluation of upper limits of normal values of antistreprolysin O
& anti-deoxyribonuclease B in Mumbai. Indian J Med Res;
SPECIMEN 119 (Suppl): 26-28, 2004.
Follow standard laboratory procedures to collect serum samples.
It is recommended to perform test immediately after sample collection. If
the test cannot be done immediately, store sample at 2- 4° C for up to 3
days or at -20° C for up to 1 months. Avoid repeated freezing and
thawing.

PROCEDURE Genuine Biosystem Private Limited

It is very important for antigen-antibody reaction needs the pre- Plot No.97 & 98, kattabomman street,
warm of both reagents and samples. Along with GB NEPHCHEM Parvathy Nagar Extension,
equipment, dry bath incubator will be provided. In that dedicated
R1, R2 and sample positions were available. Please use the respective
Old Perungalathur, Chennai - 600063, India.
positions for desired pre-warm temperature of 37°C Ph: +91-44-48681845
Email: [email protected]
GBPL/NCASO/02 9.19
website: www.genuinebiosystem.com