Quick Instruction Guide Scil Vet Abc Plus
Quick Instruction Guide Scil Vet Abc Plus
Quick Instruction Guide Scil Vet Abc Plus
Instruction Guide
for Operating the scil Vet abc Plus
Table of Contents
Start Up Cycle…………………………………………….......................……pg. 2
Reagents…………………………………………….......................………….pg.2-3
User Profiles……………………………….…………….......................……..pg.4-5
Species Selection………………………….………….......................……….pg.6-7
Status Indicator……………………………….…….......................………….pg.8
Flags…………………………………………………........................……......pg.11
Quality Control………………………………….......................….…………..pg.12-17
Calibration…………………………………….......................……….………..pg.18-19
Concentrated Cleaning…………………….......................…….…….………pg.22
Archive Management..………………………........................……..…………pg.24-27
Printer’s……………………………………........................……………………pg.28-29
Troubleshooting...................................……………………………..…………pg.30-31
877-724-5838 847-223-3371
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Scil Vet ABC Plus+ Quick Instruction Guide
Start Up Cycle
A Start Up cycle is performed every time the machine is powered on by turning on the
switch at the back of the machine. A temperature check cycle will initiate on power up and
once it passes the Start up Cycle will begin. Samples should not be run until a Start Up
has passed.
To manually initiate a Start up Cycle press the Status Button located at the top right of
the screen and then press the Green Start Up Button on the bottom right corner of the
screen.
4. Press to confirm.
5. Remove the 4 red reagent protection caps from the bottom of the reagent pack
including the red waste connector tab from the top of the reagent pack.
6. Place the pack in the compartment of the instrument with the LOT number facing
outward.
7. Plug the waste connector to the connector site on the top of the pack.
10. Enter the LOT number from the reagent pack by using the internal keyboard. It is
located on the side of the pack facing out.
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Scil Vet ABC Plus+ Quick Instruction Guide
12. Press to prime all reagents. The scil Vet abc Plus+ performs the reagent priming
automatically. This cycle takes approx. two minutes.
13. The amount of CBC’s left in the Pack will be indicated on this screen with a
percentage bar showing the percent of fluid left in the pack. (This amount should be
at 100% after the change pack procedure performed above.)
14. Select one of the main menu buttons to leave the “Reagent Replacement” menu.
1. Write the installation date on the pack to distinguish it from a new pack.
2. Make sure all 4 red caps have been removed before pack is inserted into unit.
3. Make sure there are no obstructions in the reagent pack receptacle area before
inserting the pack into the unit.
4. Verify waste connector is firmly installed and tubing is not being pinched by the top
cover or by the pack.
5. Check questionable “new” or “used” packs for fluid by placing the reagent pack on its
side so three bottom ports are visible and press a pen or screw driver into each port to
see if any fluid escapes (if pack still has fluid, it will rush out rather freely so be
prepared to have some paper towels to catch the fluid). If no fluid is coming out of any
of the three bottom ports – the pack is used up.
Make sure that you Burp your pack regularly. Remove the waste line at the top of the
reagent pack and press a pen or screw driver on the top port to release any build up of air
inside the waste port. The more the machine is used the more regularly you will have to
burp the pack.
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Scil Vet ABC Plus+ Quick Instruction Guide
User Profiles
There are two different user profiles available to work with the scil Vet abc Plus+:
• User profile “VET-TECH” to run an analysis. This profile is not password
protected.
• User profile “Scientist” to run analysis, and manage the system. This profile is
password protected.
The “tech” profile is reserved for scil service technicians.
4. Press to enter.
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Scil Vet ABC Plus+ Quick Instruction Guide
1. Press .
6. Press to enter.
New user profiles can be created by the "Scientist" profile if desired. Please see the User
Manual for more details.
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Scil Vet ABC Plus+ Quick Instruction Guide
Species Selection
The scil Vet abc Plus+ comes with the standard settings installed for dog, cat, horse, pig,
sheep, cattle, rabbit, mouse and rat. Dog, cat and horse are the only species with
differentials.
The order of the animal icons in the screen ‘Species Selection’ can be changed.
Species that are not used can be hidden.
Additional Functions:
1. Press to delete a species
*Note-this function will permanently remove the deleted species from the analyzer*
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Scil Vet ABC Plus+ Quick Instruction Guide
2. Press .
Button Description:
Additional Function:
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Scil Vet ABC Plus+ Quick Instruction Guide
Status Indicator
Depending on the status of the instrument, the status button will show
different colors
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Scil Vet ABC Plus+ Quick Instruction Guide
Running a Measurement
1. Press to open the internal keyboard.
2. The sample ID is generated automatically by the instrument.
The ID number is displayed in the “Sample ID” field. The
sample ID is composed of the prefix AUTOSID and the
sequential number of each identification.
3. Press “Animal ID” to enter the identification number of the
animal by using the virtual keyboard.
Optional data can be keyed in, such as animal name, owner name, date of birth of
patient. This data will be saved under the animal ID.
4. Once you have completed, press to close this menu and return to the “Analyze”
menu.
The dialog box “Sample Analysis” is displayed, which gives further information to run
the analysis.
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Scil Vet ABC Plus+ Quick Instruction Guide
Reference cycle
If too much time has elapsed between two measurements, a dialog screen is displayed
If the above mentioned screen appears – press the sample bar to initiate a hemoglobin
reference run
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Scil Vet ABC Plus+ Quick Instruction Guide
Flags
* - following either WBC, RBC HCT or PLT -- system analyzed the sample 3 times but all
3 counts differed and were outside the system’s precision limits (see below). Result
should be verified by repeating the sample.
$ - between the test result and the units -- 3 counts were made and 2 were within the
system’s precision limits (see below). The result can be accepted.
! - next to the HGB result -- the difference between HGB blank done for this analysis and
the HGB blank done for the previous analysis was outside the system’s precision limits.
Instrument provides a result based on the previous HGB blank. Result can be accepted.
If flag persists for more than 3 consecutive times, see section 9.2.3.3 in the scil Vet abc
manual to run checkup procedure.
---D or < printed instead of a result -- linearity range for that parameter has been
exceeded (see below). Repeat using a 1:2 dilution with saline solution. Multiply your
results by 3.
MIC following PLT Flags -- microcytes present in the platelet measurement zone. Verify
result with manual count.
SCH following PLT Flags -- schistocytes or platelet aggregates present in the platelet
measuring zone. Review slide before reporting results.
SCL following PLT Flags -- small particles in the 2 - 3 fl zone.Reanalyze sample. If flag
persists, perform an automatic cleaning cycle and repeat again. If flag still persists,
perform manual platelet count.
AG1 and AG2 – Flags for big Leucocytes aggregates (> 1,5 % / 2,5 % of total
Leucocytes). Mix sample properly and repeat measurement.
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Scil Vet ABC Plus+ Quick Instruction Guide
Quality Control
Required:
One 2.5-mL vial of Minotrol, Blood Control. (Low, Normal or High)
Quality Control Target Values must be loaded on the Vet ABC Plus for the selected
control vial.
Caution
The Blood Controls;
• Must be stored in a refrigerator between 2 and 8 degrees Celsius.
• Can never be frozen at anytime.
• Cannot be left out for more than 15 minutes.
• Should not be held in a hand while waiting for control results, may get too warm
• Do not use a Blood Rocker.
Blood Control Preparation
1. Read this entire Quality Control Procedure.
2. Remove Blood Control Vial from the refrigerator and roll the blood control vial in
your hands until the vial is at room temperature.
3. Invert the vial back and forth slowly, paying attention to the concentration of
cells at the bottom of the vial. There should not be a concentration of cells at
the bottom of the vial when the vial is properly mixed. DO NOT SHAKE
4. The Low control mixes fairly easily while the Normal and High controls contains
more cells and requires a longer inversion period (3 minutes of slow inversion).
5. When running multiple Blood Controls, always invert the vial 20 times before
each sample. Do not continue to roll the vial in your hands.
**All blood control vials are stable until the printed expiration date if unopened and stored
in a refrigerator.
Quality Control Handling Recommendations
1. Write the date on the control vial and cover it with tape to prevent it from
rubbing off when vial is first opened.
2. The control vial should be mixed for a minimum of 3 minutes prior to running a
quality control test.
3. If the control vial has been dropped or frozen it will damage the cells and cause
aberrant results and flags. (ex. $, *, MIC, etc...)
4. Clean the internal and external threads of the cap and vial to prevent the blood
from drying and flaking off into the control vial.
5. If a control vial has been left out too long or is too warm it will distort the HGB
result.
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Scil Vet ABC Plus+ Quick Instruction Guide
Once the control is opened it is good for 16 days in a refrigerator if proper handling is
followed.
Quality Control Procedure
Open the sub menu “Quality control / QC” by pressing the “QC” tab. The following screen
is displayed:
Control status
QC result history
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Scil Vet ABC Plus+ Quick Instruction Guide
Use only Minotrol-16 Vet controls with the scil Vet abc Plus+.
Minotrol-16 is available in three different measurement ranges (low, normal, high).
The scil Vet abc Plus+ target can store target values for up to three different controls.
The target ranges of each quality control can be entered manually or imported via USB
key. To import via USB flashdrive, the information must be downloaded from the internet.
2. Select in the file of the lot number you are currently using (e.g. MX079).
3. Click on the download icon and select the "save as“ function to save the file to the
USB flash drive. *Note-this file should be saved as a .cvs file*
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Scil Vet ABC Plus+ Quick Instruction Guide
The file on the USB flashdrive, imported from scil animal care
company website contains the lot number, name, expiration date
and the target values for the related control. *Note: there should be
only 1 control file on the USB flashdrive – if an older control file exist
please delete it before trying to upload the control information into
the Vet ABC Plus+.*
Please insert your USB key into the front slot of the instrument.
6. Select the control lot to be imported from the “QC Name” list, by using the arrow
buttons, then press .
The data import from the USB flash drive is started.
7. When the import is completed, control information is displayed in the “Control
Modification Screen”.
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Scil Vet ABC Plus+ Quick Instruction Guide
8. Confirm the displayed data on the screen such as lot number and expiration date
matches the data on the control insert sheet.
The scil Vet abc Plus+ defaults a control measurement every 24 hours. If this
request is ignored, an alarm is activated.
To avoid this demand, the interval between two QC requests can be set on
9999.
9. Press to open the internal keyboard.
10. Change the internal QC time limit to 9999.
control reference sheet under the column "scil Vet abc Plus+".
5. Change the internal QC time limit to 9999.
The scil Vet abc Plus+ requires a control measurement
every 24 hours as a default setting.
If this request is ignored, an alarm is activated.
To avoid this demand, the interval between two QC
requests can be set on 9999.
6. Press to enter.
8. Press to enter.
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Scil Vet ABC Plus+ Quick Instruction Guide
3. Press to enter.
**The scil Vet abc Plus+ switches automatically to Quality
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Scil Vet ABC Plus+ Quick Instruction Guide
Calibration
The scil Vet ABC Plus should be calibrated every six months.
Before Calibrating
DO NOT remove the Calibration Fluid from the refrigerator until step 6 is complete and you
understand the instructions.
Do not follow the instructions that come with the calibrator. Only follow these instructions
to complete the calibration.
When calibrating your machine the measured value of each parameter may not reflect the
target values indicated on the sheet provided with the calibrator vial.
Your goal is not to recreate the values on the target sheet provided with the calibrator vial;
the targets are used as reference for calibrating the instrument.
If you are in the middle of doing a calibration and need to call technical support make sure
you return your calibration fluid to the refrigerator.
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Scil Vet ABC Plus+ Quick Instruction Guide
4. Press the Internal Keyboard Button and fill in all required data. This information
comes with your calibrator vial on the reference sheet provided.
5. Remove your calibrator vial from the refrigerator and follow the Blood
Preparation instructions found in the Daily Quality Control instructions
6. Press the OK Button the calibration cycle will begin.
7. The needle will come down. Aspirate the sample by placing the vial under the
needle and press the sample bar.
9. You can press the Cancel Button to discard a run and re-run the sample if you
feel that the results are not satisfactory.
If during any Run, the results are displayed and there is no OK Button, the machine won’t
accept that run due to a flag on one of the parameters. Press the Cancel Button to
proceed. You will have to re-run the sample.
If your calibration fails, return your calibrator vial to the refrigerator immediately and
perform a concentrated cleaning procedure. Keep your print out and all related information
and call technical support line for further instructions if your calibration continues to fail.
You may be asked to fax your print out to the technical support fax line.
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Scil Vet ABC Plus+ Quick Instruction Guide
It is very important to follow the shut down procedure for your Vet ABC Plus+.
This shutdown cycle will rinse the analyzer and prevent debris in the tubing system. The
scil Vet abc Plus+ should never be switched off without performing the shutdown cycle.
The shutdown cycle will rinse with the chambers with proteolytic cleaning reagent
(contained in the reagent pack) into the measuring chambers.
Protein debris in the chambers and in the tubing are peeled off and will be washed out with
the next startup cycle.
The shutdown takes approx. 1 minute.
Switch off the analyzer with power switch on back of the instrument.
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Scil Vet ABC Plus+ Quick Instruction Guide
3. Use a dampened cotton swab to clean around the rim of each chamber. DO NOT
go past the white clips holding the chamber with the cotton swab – doing so may
damage the sensitive measuring aperatures.
4. Once both chambers have been clean slide the black cover back on (make sure the
cover is flush against the frame and is not pinching any tubing), tighten allen screw
and proceed to the Concentrated Cleaning Procedure.
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Scil Vet ABC Plus+ Quick Instruction Guide
and the instructions below: (warning – you will get a red status indicator if you
abort out of the Concentrated Cleaning process).
The concentrated cleaning takes 10 minutes. Perform a startup cycle to complete the
concentrated cleaning; afterwards you can analyze patient samples as usual.
WBC RBC
Chamber Chamber
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Scil Vet ABC Plus+ Quick Instruction Guide
4. Select the SERV. tab to open that page and then press .
7. Pour 5 ml of 100% Clorox bleach into both the WBC and RBC chambers.
8. Select the BACKFLUSH blue button then press to execute and Backflush 20
TIMES.
9. Let the unit sit as is for a minimum of 30 minutes, but longer is better. ** Please
note that the longer the bleach sit in the chambers/lines the better chance of clot
removal—OVERNIGHT is ideal!** Leave machine on if an OVERNIGHT cleaning
is performed.
11. Select the CONCENTRATED CLEANING blue button and run this once.
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Scil Vet ABC Plus+ Quick Instruction Guide
Archive Management
In the screen ‘Archive’ the last archiving operation date for each kind of archive is
displayed.
If a message is displayed in the status area that an archiving is necessary, you have
several options:
1. Create an archive.
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Scil Vet ABC Plus+ Quick Instruction Guide
Archive ‘Logs’
All information about alarms and the analysis list of the scil Vet abc Plus+ is saved in
the archive ‘Logs‘ This data can be stored on the USB key. All data can be accessed
from the technical service section and allows precise analysis of diagnostic errors.
The scil Vet abc Plus+ requires this archive be created at intervals of 4 weeks.
Archive ‘QC’
All results from control analysis of the scil Vet abc Plus+ are saved in the archive ‘QC‘.
This data can be stored on the USB key. The scil Vet abc Plus+ will ask to create this
archive if a new QC-Lot was stored or a new QC-Measurement was performed.
Archive ‘Analysis’
In the archive ‘Analysis‘ all patient results are saved.
This data can be stored on the USB key.
The scil Vet abc Plus+ requires creation of an archive if more than 990 results are listed
in the history.
The patient results are saved in the archive ‘Analysis’ as an .xml-file. The .xml-files will
be subsumed in a .tar-file.
To view this data the special viewer software is needed, which is included with the
USB flashdrive, can be installed on your PC. You can also download the viewer
software from the scil website. www.scilvet.nl
Creating an Archive
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Scil Vet ABC Plus+ Quick Instruction Guide
5. Insert the USB key into the front slot of the instrument.
Access archived "Analyses" results with the Viewer Software “MICROS ES-ESV60”
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Scil Vet ABC Plus+ Quick Instruction Guide
This window will allow the user to select the archived files to open on the
computer.
3. Select the archived file from the Explorer window to open it.
• Result archives are saved as .tar files,
Ex. “ar-904EVOH00034-result-20090716121005.tar”.
• QC archives are saved as .xml files,
Ex. “ar-812EVOH00073-controls-20090318164150.xml.
All results that are saved in the open archived files are
listed on the left side.
Click on the result in the list to open it.
Additional Functions
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Scil Vet ABC Plus+ Quick Instruction Guide
Printers
The scil Vet ABC Plus will work with many different printer name brands and types. The
printer must come standard with PCL emulation. The printer currently sold with the
instrument is the Brother HL-5340D laser printer.
Drivers
The scil Vet ABC Plus is pre loaded with 11 different types of drivers. Some printers work
with multiple drivers while others are driver specific. Two of the drivers are DeskJet drivers
and should be used when an inkjet printer is connected.
Brother HL-5340D – ljet4
Oki Data B4400 - laserjet
Most HP inkjets - deskjet500, deskjet
Connection
To connect a Local printer a USB cable must be used. To connect a remote printer using
an Ethernet cable, you must consult your Network Administrator and have the scil Vet ABC
Plus connected to your network. You can also call Technical Support for more information
regarding the remote printer feature.
Printer Button
Any time you see the Printer Button you can press it and it will print the current screen of
data. You can reprint any results, QC results, logs, configuration report of the instrument
etc.
Printer List
A list of compatible printers can be requested from your scil representative or you may
contact the main office to request the list.
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Scil Vet ABC Plus+ Quick Instruction Guide
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Scil Vet ABC Plus+ Quick Instruction Guide
Troubleshooting
Instrument Alarms - displayed when a cycle fails.
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Scil Vet ABC Plus+ Quick Instruction Guide
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Scil Vet ABC Plus+ Quick Instruction Guide
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