Au2016354228b2 Seed Coating

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(12) STANDARD PATENT (11) Application No.

AU 2016354228 B2
(19) AUSTRALIAN PATENT OFFICE

(54) Title
Seed coating composition

(51) International Patent Classification(s)


A01N 25/00 (2006.01) A01N 43/78 (2006.01)
A01N 37/46 (2006.01) A01P 3/00 (2006.01)
A01N 43/36 (2006.01) A01P 7/00 (2006.01)
A01N 43/56 (2006.01)

(21) Application No: 2016354228 (22) Date of Filing: 2016.11.08

(87) WIPO No: W017/081535

(30) Priority Data

(31) Number (32) Date (33) Country


EP15193624.2 2015.11.09 EP

(43) Publication Date: 2017.05.18


(44) Accepted Journal Date: 2020.10.15

(71) Applicant(s)
Incotec Holding B.V.

(72) Inventor(s)
Bueno, Amy Nicole;Meyer, Terry Euclaire;Anthony, Renil John

(74) Agent / Attorney


Houlihan2 Pty Ltd, Level 1, 70 Doncaster Road, BALWYN NORTH, VIC, 3104, AU

(56) Related Art


WO 03003812 Al
WO 2013150261 Al
EP 0080999 Al
WO 2014/062660 Al
WO 2005/077169 Al
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT)
(19) World Intellectual Property
Organization
International Bureau
(10) International Publication Number
(43) International Publication Date W O 2017/081535 A1
18 May 2017 (18.05.2017) W IPOIPCT

(51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every
A01N25/00 (2006.01) A01N43/78 (2006.01) kind of national protection available): AE, AG, AL, AM,
A01N37/46 (2006.01) A01P 7/00 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY,
A01N 43/36 (2006.01) A01P 3/00 (2006.01) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM,
A01N 43/56 (2006.01) DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT,
HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR,
(21) InternationalApplicationNumber: KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME,
PCT/IB2016/001661 MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ,
(22) International Filing Date: OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA,
8 November 2016 (08.11.2016) SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM,
TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM,
(25) Filing Language: English ZW.
(26) Publication Language: English (84) Designated States (unless otherwise indicated, for every
(30) Priority Data: kind of regional protection available): ARIPO (BW, GH,
EP15193624.2 9 November 2015 (09.11.2015) EP GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ,
TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU,
(71) Applicant: INCOTEC HOLDING B.V. [NL/NL]; West- TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE,
einde 107, 1601 BL Enkhuizen (NL). DK, EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU,
(72) Inventors: BUENO, Amy, Nicole; 1000 Sarasota Drive, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK,
Lafayette, IN 47909 (US). MEYER, Terry, Euclaire; SM, TR),OAPI(BF, BJ,CF, CG, CI, CM,GA,GN,GQ,
2507 Redfree Drive, West Lafayette, IN 47906 (US). AN- GW,KM,ML,MR,NE,SN,TD,TG).
THONY, Renil, John; 3308 Peppermill Drive, Apt. IC, Published:
West Lafayette, IN 47906 (US). with internationalsearch report (Art. 21(3))

(54) Title: SEED COATING COMPOSITION

(57) Abstract: The invention is directed to a seed coating composition comprising one or more active ingredients, to a method and
N use for improving the bio-efficacy of an active ingredient in a seed coating, and to a coated seed. The seed coating composition of
the invention further comprises a wax and/or polymeric carbohydrate and wherein said seed coating composition has an amount of
polymeric binder excluding wax and polymeric carbohydrate of 10 % or less by total weight of the wax and polymeric carbohydrate
together.
WO 2017/081535 PCT/IB2016/001661
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SEED COATING COMPOSITION

This application is related to, and claims the benefit of priority of, European
Application No. EP 15193624.2, entitled SEED COATING COMPOSITION, filed on 9
November 2015, the contents of which are incorporated herein by reference in their
5 entirety for all purposes.
The invention is directed to a seed coating composition comprising one or
more active ingredients, to a method for improving the bio-efficacy of an active
ingredient in a seed coating, and to a coated seed.
Plant seed is often coated before sowing, for example, to protect seeds from
10 damage during handling and/or to improve handling properties. Seeds are often coated
to provide useful substances (active ingredients) to the seed and the seedlings upon
germination, for example, plant nutrients, growth stimulating agents, and plant
protective products. An important advantage of providing active ingredients in a seed
coating is that it allows for a precise and controlled release and dose per seedling.
15 Typical seed coating methods include film coating, pelleting and encrusting of seed.
The seed coating can contain many ingredients, and each of these
ingredients has their advantages and disadvantages. One of the disadvantages that
may occur when applying active ingredients in a coating composition is that, depending
on the nature of the coating, the type of active ingredient, and the solubility thereof,
20 the active ingredient may be retained in the coating or released more slowly than
required and the resulting efficacy of the active ingredient for the seed or plant may be
decreased. In particular, the rise of hydrophobic and/or water insoluble active
ingredients brings new challenges for formulators of seed coating compositions. The
active ingredients should still have sufficient mobility in order to adequately perform
25 their function, but on the other hand the seed coating itself should have the
conventional and desirable seed coating properties, such as water permeability, and
should not negatively affect seed germination.
Some attempts have been made in the art to include hydrophobic active
ingredients in a seed coating.
30 WO-A-2001/010212, for instance, describes a composition that may
comprise a hydrophobic active ingredient, wherein the composition is based on a
water-in-oil polymer dispersion.
In spite of these prior attempts, there is still a need in the art to provide a
seed coating composition which facilitate the overall movement of active ingredients,
35 while preserving the usual important properties of the seed coating.
WO 2017/081535 PCT/IB2016/001661
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An object of the invention is to address this need in the art and overcome
disadvantages of prior art seed coating compositions.
The inventors found that this object can, at least in part, be met by using
special seed coating formulations as described herein.
5 Accordingly, in a first aspect the invention is directed to a seed coating
composition comprising one or more biologically active ingredients and a wax and/or
polymeric carbohydrate, wherein said seed coating composition has an amount of
polymeric binder excluding wax and polymeric carbohydrate of 10 % or less by total
weight of the wax and polymeric carbohydrate together.
10 In a second aspect, the invention is directed to a method for improving the
bio-efficacy and/or systemic uptake by plants of one or more active ingredients in a
seed coating composition, comprising preparing a seed coating composition comprising
one or more biologically active ingredients and a wax and/or polymeric carbohydrate,
wherein said seed coating composition has an amount of polymeric binder excluding
15 wax and polymeric carbohydrate of 10 % or less by total weight of the wax and
polymeric carbohydrate together, and applying said seed coating composition to seed.
In a third aspect, the invention is directed to the use of a seed coating
composition comprising one or more biologically active ingredients and a wax and/or
polymeric carbohydrate, wherein said seed coating composition has an amount of
20 polymeric binder excluding wax and polymeric carbohydrate of 10 % or less by total
weight of the wax and polymeric carbohydrate together, to reduce the
bridging/clumping of the coated seeds and/or reduce the coefficient of friction between
the coated seeds and improve flowability during processing.
It was surprisingly found that the seed coating composition of the invention
25 allows for improved bio-efficacy of the active ingredient. Active ingredients were better
released from the seed coating to perform their desired effect, causing better
bio-efficacy. At the same time, the unconventional seed coating compositions of the
invention surprisingly are able to retain desirable seed coating properties such as
water-permeability, good abrasion resistance, low dust emissions, short drying time,
30 good flow ability and plant ability, low clumping, good cosmetics and/or coverage.
The term "seed" as used in this application is meant to refer in particular to
the ripened ovule of gymnosperms and angiosperms, which contains an embryo
surrounded by a protective cover. In particular, the term covers cereal kernels. The
protective cover can comprise the seed coat (testa). Some seeds comprise a pericarp
35 or fruit coat around the seed coat. In particular, when this layer is closely adhered to
the seed, as in cereal kernels, it is in some cases referred to as a caryopsis or an
achene. As used in this application, the term "seed coat" is meant to include a
WO 2017/081535 PCT/IB2016/001661
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caryopsis or an achene. In practical terms, the term "seed" includes but is not
restricted to anything that can be planted in agriculture to produce plants, including
pelleted seeds, true seeds, plant seedlings, rootstock, regenerable and plant forming
tissue, and tubers or bulbs.
5 The term "coating" as used in this application, is meant to refer to applying
material to a surface of a seed, for instance as a layer of a material around a seed.
Coating includes film coating, pelleting, and encrusting or a combination of these
techniques. Pellets obtained with pelleting are also known as seed pills. The coating is
preferably applied over substantially the entire surface of the seed, such as over 90

%
10 or more of the surface area of the seed, to form a layer. However, the coating may be
complete or partial, for instance over 20 % or more of the surface area of the seed, or
50 % or more.
The term "seed coating composition" as used in this application is meant to
refer to a composition, preferably aqueous composition, to be used for coating of seed,
15 possibly after combination with other additives, such as plant protective product
formulations, diluents such as water, nutrients, and/or inoculants such as beneficial
fungi or bacteria.
The term "plant enhancing agent" as used in this application is meant to
refer to any component that is directly or indirectly advantageous for a plant or a plant
20 seed, for instance through a biological effect on the plant, seed, or on organisms
harmful for a plant such as fungi, pests and insects. Plant enhancing agents include
plant protective products, safeners, growth promoters, growth regulators, and the like.
The seed is a plant seed, for example a seed of an agricultural crop, a
vegetable seed, a herb seed, a wildflower seed, an ornamental seed, a grass seed, a
25 tree seed, or a bush seed.
Preferably, the plant seed is of an agricultural crop. The seed may be of the
order of Monocotyledoneae or of the order of Dicotyledoneae. Suitable seeds include
seed of soybean, cotton, corn, peanut, maize, wheat, barley, oat, rye triticale, mustard,
oil seed rape (or canola) sunflower, sugar beet, safflower, millet, chicory, flax,
30 rapeseed, buckwheat, tobacco, hemp seed, alfalfa, signal grass, clover, sorghum, chick
pea, beans, peas, vetch, rice, sugar cane, and linseed. Examples of suitable vegetable
seeds include asparagus, chives, celery, leek, garlic, beetroot, spinach, beet, curly kale,
cauliflower, sprouting broccoli, savoy cabbage, white cabbage, red cabbage, kohlrabi,
Chinese cabbage, turnip, endive, chicory, water melon, melon, cucumber, gherkin,
35 marrow, parsley, fennel, pea, beans, radish, black salsify, eggplant, sweet corn, pop
corn, carrot, onion, tomato, pepper, lettuce, snap bean, cucurbit, shallot, broccoli,
Brassica, and Brussels sprout.
WO 2017/081535 PCT/IB2016/001661
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Preferably, the plant seed is capable of germinating. Optionally, the seed


may be deprived of husk (so-called husked seed or de-hulled seed). The seed may be
primed or not primed (having been subjected to a treatment to improve the
germination rate, e.g. osmopriming, hydropriming, matrix priming).
5 The amount of polymeric binder in seed coating composition excluding wax
and/or polymeric carbohydrates can be 10 % or less by total weight of the wax and
polymeric carbohydrates together, preferably, the amount of polymeric binder
excluding wax and/or polymeric carbohydrates is 8 % or less by total weight of the wax
and polymeric carbohydrates together, such as 6 % or less, 4 % or less, 2 % or less, or
10 1 % or less. More preferably, the seed coating composition is essentially free from
polymer binder excluding wax and/or polymeric carbohydrates. This means that other
than wax or polymeric carbohydrates or a combination of wax and polymeric
carbohydrates essentially no polymeric binders are present in the seed coating
composition. Trace amounts, though, may still be present, such as amounts of 0.1

%
15 or less by total weight of the wax and polymeric carbohydrates together, or 0.05 % or
less. Most preferably, the seed coating composition is free from polymeric binder
excluding wax and/or polymeric carbohydrates.
Various polymeric carbohydrates exist. Examples thereof include starch,
cellulose (and derivatives thereof), and dextrins (including cyclodextrins). In an
20 embodiment, the amount of polymeric binder in seed coating composition excluding
wax and/or starch and/or cellulose can be 10 % or less by total weight of the wax and
polymeric carbohydrates together, preferably, the amount of polymeric binder
excluding wax and/or starch and/or cellulose is 8 % or less by total weight of the wax
and polymeric carbohydrates together, such as 6 % or less, 4 % or less, 2 % or less, or
25 1 % or less. In a further embodiment, the amount of polymeric binder in seed coating
composition excluding wax and/or starch can be 10 % or less by total weight of the
wax and polymeric carbohydrates together, preferably, the amount of polymeric binder
excluding wax and/or starch is 8 % or less by total weight of the wax and polymeric
carbohydrates together, such as 6 % or less, 4 % or less, 2 % or less, or 1 % or less.
30 In accordance with the invention, the said seed coating composition can
comprise the wax and/or polymeric carbohydrates in an amount of 10-50 % by total
weight of the wax and polymeric carbohydrates together (i.e. the sum of total wax and
starch in the seed coating composition), preferably in an amount of 10-45 %, more
preferably in an amount of 20-40 %.
35 The wax may be selected from the group consisting of natural wax, mineral
wax and synthetic wax or a combination thereof. Preferably, the wax is selected from
the group consisting of polyethylene wax, carnauba wax, paraffin wax, polypropylene
WO 2017/081535 PCT/IB2016/001661
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wax, oxidised polyethylene wax, montan wax, ceresin wax, ozocerite, peat wax,
Fischer-Tropsch wax, amide wax, ethylene-acrylic acid wax, polyolefin wax, ethylene
bis stearamide wax, bees wax, lanolin wax, sugar cane wax, palm wax, and vegetable
wax. It is also possible that mixtures of two or more waxes are present in the seed
5 coating composition of the invention. In a preferred embodiment, the wax is selected
from the group consisting of polyethylene wax, Fischer-Tropsch wax, and carnauba
wax. The wax can be an anionic wax, a non-ionic wax or a cationic wax. Most
preferably, the wax can be anionic or non-ionic. Cationic waxes may give rise to
flocculation problems when the seed coating composition is combined with anionically
10 stabilised active ingredients.
The starch may be derived from a plant source including corn/maize, rice,
tapioca, and potato. Derivatives of starch obtained from these plant sources that are
modified through chemical/physical processes including but not limited to hydrolysis,
esterification, etherification, crosslinking, grafting, oxidation and acetylation or a
15 combination of these are also considered here. Also included are starches that are
modified through breeding and genetics such as waxy maize starch, resistant starch
and others.
The cellulose may be derived from a plant source or recycled material.
Derivatives of cellulose synthesized using esterification and etherification are also
20 considered in this embodiment. Additionally, hemicellulose derived from plant materials
and its derivatives are included here.
If a polymeric binder other than wax or starch is present, then the binder
may, for example, be selected from the group consisting of polyvinyl acetates,
polyvinyl acetate copolymers, polyvinyl alcohols, polyvinyl alcohol copolymers,
25 polyurethane, celluloses (including ethylcelluloses, methylcelluloses,
hydroxymethylcelluloses, hydroxypropylcelluloses, carboxymethylcelluloses, and
hydroxymethylpropylcelluloses), polyvinylpyrrolidones, dextrins, maltodextrins,
polysaccharides (other than starch), fats, oils, proteins, gum arabics, shellacs,
vinylidene chloride, vinylidene chloride copolymers, calcium lignosulphonates,
30 polyacrylates, acrylic copolymers, polyvinylacrylates, zeins, casein, gelatine, chitosan,
pullulan, polyethylene oxide, polyethylene glycol, ethylene vinylacetate, acrylamide
polymers, acrylamide copolymers, polyhydroxyethyl acrylate, methylacrylimide
monomers, poly(N-vinylacetamide), sodium alginate, polychloroprene and syrups.
These binders may be used alone or in combination of two or more. Preferred binders
35 can be selected from the group consisting of polyvinyl acetates, polyvinyl alcohols,
hydroxypropylmethylcellulose, polysaccharides (other than starch), proteins,
polyethylene glycol, polyvinyl pyrrolidones, and polyacrylates.
WO 2017/081535 PCT/IB2016/001661
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The seed coating composition comprises one or more active ingredients


(including plant enhancing agents, in particular plant protective products (also referred
to as PPPs). Suitable examples of active ingredients, in particular plant enhancing
agents, are fungicidal agents, bactericidal agents, insecticidal agents, nematicidal
5 agents, molluscicidal agents, biologicals, acaricides or miticides, pesticides, and
biocides. Further possible active ingredients include disinfectants, micro-organisms,
rodent killers, weed killers (herbicides), attracting agents, (bird) repellent agents, plant
growth regulators (such as gibberellic acid, auxin or cytokinin), nutrients (such a
potassium nitrate, magnesium sulphate, iron chelate), plant hormones, minerals, plant
10 extracts, germination stimulants, pheromones, biological preparations, etc.
In accordance with the invention, it is preferred that the one or more active
ingredients comprise at least one hydrophobic and/or water insoluble active ingredient.
The amount of active ingredient applied, of course, strongly depends on the
type of active ingredient and the type of seed used. Usually, however, the amount of
15 one or more active ingredients is in the range of 0.001-200 g per kg of the seed. The
skilled person is able to determine suitable amounts of active ingredient depending on
the active ingredient and the type of seed used. It is common practice for the skilled
person to use and follow the advice of the active ingredient suppliers (e.g., BASF,
Bayer, Syngenta, DuPont, etc.), such as by using technical data sheets and/or follow
20 recommendations.
Typical fungicidal agents include Captan
(N-trichloromethyl)thio-4-cyclohexane-1,2-dicarboximide), Thiram
tetramethylthioperoxydicarbonic diamide (commercially available as Proseed T M

Metalaxyl (methyl-N-(2,6-dimethylphenyl)-N-(methoxyacetyl)-d,l-alaninate),
25 Fludioxonil (4-(2,2-difluoro-1,3-benzodioxol-4-yl)-1-H-pyrrol-3-carbonitril;
commercially available in a blend with mefonoxam as Maxim' XL), difenoconazole
(commercially available as DividendTM 3FS), carbendazim iprodione (commercially
available as RovralTM), ipconazole (commercially available as Rancona from Arista,
formerly Agriphar or Chemtura), mefonoxam (commercially available as ApronTM XL),
30 tebuconazole, carboxin, thiabendazole, azoxystrobin, prochloraz, prothioconazole
(commercially available as Redigo from Bayer), sedaxane (commercially available as
Vibrance from Syngenta), cymoxanil (1-(2-cyano-2-methoxyiminoacetyl)-3-ethylurea),
fludioxonil, a mixture of metalaxyl, cymoxanil and fludioxonil commercially available as
Wakil from Syngenta, and oxadixyl (N-(2,6-dimethylphenyl)-2-methoxy
35 N-(2-oxo-3-oxazolidinyl) acetamide). A fungicide can be included in the seed coating
composition in an amount of 0.0001-10 % by total weight of the coated seeds.
WO 2017/081535 PCT/IB2016/001661
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Typical bactericidal agents include streptomycin, penicillins, tetracyclines,


ampicillin, and oxolinic acid.
Typical insecticidal agents include pyrethroids, organophosphates,
caramoyloximes, pyrazoles, amidines, halogenated hydrocarbons, neonicotinoids, and
5 carbamates and derivatives thereof. Particularly suitable classes of insecticides include
organophosphates, phenylpyrazoles and pyrethoids. Preferred insecticides are those
known as terbufos, chlorpyrifos, fipronil, chlorethoxyfos, tefluthrin, carbofuran,
imidacloprid, and tebupirimfos. Commercially available insecticides include imidacloprid
(commercially available as GauchoTM), and clothianidin (commercially available from
10 Bayer as PonchoTM), thiametoxam (commercially available from Syngenta as CruiserTM)
thiacloprid (commercially available as Sonido from Bayer), Cypermetrin (commercially
available from Chemtura as Langis), methiocarb (commercially available as Mesurol
from Bayer), fipronil (commercially available from BASF as RegentT M ),
chlorantraniliprole (also known as rynaxypyr, 5-bromo-N-[4-chloro-2-methyl
15 6-(methylcarbamoyl)phenyl]-2-(3-chloropyridin-2-yl)pyrazole-3-carboxamide,
commercially available as Coragen* from DuPont) and cyantraniliprole (also known as
cyazypyr, 3-bromo-1-(3-chloro-2-pyridyl)-4'-cyano-2'-methyl
6'-(methylcarbamoyl)pyrazole-5-carboxanilide).
Commercially available nematicidal agents include abamectin (commercially
20 available from Syngenta as AvictaTM) thiodicarb (commercially available from Bayer as
AerisTM).

Typical molluscicidal agents include metaldehyde (commercially available


from Lonza as Meta®) or niclosamid (commercially available from Bayer as
Bayluscide*), Cyazypir and Rynaxypir (available from DuPont).
25 Examples of suitable biologicals include bacilli, Trichoderma, and the like,
which have been identified as seed treatment materials to protect plants and/or
enhance their health and/or productive capacity.
These lists are not exhaustive, new active ingredients are continuously
developed and can be incorporated in the seed coating composition. Coating
30 formulations are often packaged, stored and/or transported and only thereafter
combined with formulations of such plant enhancing agents.
The seed coating composition of the invention can comprise further
components such as one or more selected from a wetting and dispersing additive
(sometimes also referred to as pigment dispersant), a filler, a solvent, a thickener, a
35 colorant, an anti-foaming agent, a preservative, a surfactant, a slip additive and an
effect pigment.
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Coating formulations are typically prepared by mixing one or more of the


components with a suitable binder and/or wax, packaging, storing and/or transporting
and are only thereafter combined with formulations of plant enhancing agents.
A wetting and dispersing agent can aid in mixing inorganic particles in the
5 seed coating composition. Suitable wetting and dispersing additives include ionic and
non-ionic products and include solutions of organo-modified polyacrylates,
polyacrylates, sodium polyacrylate, polyurethane, phosphoric acid ester, star polymers,
and/or modified polyethers. The wetting and dispersing additive can, for instance, be
present in some embodiments of the seed coating composition of the invention in an
10 amount of 0-40 % by total weight of the inorganic particles, such as 0.10-10 %.
Suitable thickeners include agar, carboxy methylcellulose, carrageen, chitin,
fucoidan, ghatti, gum arabic, karaya, laminaran, locust bean gum, pectin, alginate,
guar gum, xanthan gum, diutan gum, and tragacanth, bentonite clays, HEUR
(hydrophobically modified, ethoxylated urethane) thickeners, HASE (hydrophobically
15 modified, alkali-swellable emulsion) thickeners and polyacrylates. Gums are generally
preferred because of their low cost, availability and superior ability to enhance the
physical characteristics of the resultant film.
Examples of colourants include dyes or pigmented dispersions. Examples of
suitable dyes include: anthraquinone, triphenylmethane, phthalocyanine and
20 derivatives thereof, and diazonium salts. Pigment dispersions can contain pigments
such as pigment red 112 (CAS No. 6535-46-2), pigment red 2 (CAS No. 6041-94-7),
pigment red 48:2 (CAS No. 7023-61-2), pigment blue 15:3 (CAS No. 147-14-8),
pigment green 36 (CAS No. 14302-13-7), pigment green 7 (CAS No. 1328-53-6),
pigment yellow 74 (CAS No. 6358-31-2), pigment orange 5 (CAS No. 3468-63-1),
25 pigment violet 23 (CAS No. 6358-30-1), pigment black 7 (CAS No. 97793-37-8), and
pigment white 6 (CAS No. 98084-96-9). The colourant may be present in the seed
coating composition in an amount of 0-50 % by total weight of the seed coating
composition, such as 1-10 %.
Examples of suitable antifoaming agents include polyethylene glycol,
30 glycerine, mineral oil defoamers, silicone defoamers, and non silicone defoamers (such
as polyethers, polyacrylates), dimethylpolysiloxanes (silicone oils), arylalkyd modified
polysiloxanes, polyether siloxane copolymer containing fumed silica. The antifoaming
agent may be present in some embodiments of the seed coating composition in an
amount of at least 1 ppm by weight, or 0.1-0.3 % by total weight of the seed coating
35 composition.
Examples of suitable effect pigments include pearlescent pigment in
different particle sizes. Effect pigments having a particle size of 15 pm or less, or a
WO 2017/081535 PCT/IB2016/001661
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particle size of 60 pm or less are commonly used. The particle size of the effect
pigments is normally not more than 200 pm, preferably not more than 100 pm.
Usually, the particle size of the effect pigment is 1 pm or more. Another effect pigment
can be aluminium. All effect pigments are commonly used to create a nice cosmetic
5 look on the seeds.
A biocide can be included in some embodiments of the seed coating
composition for instance as preservative, in order to prolong the shelf life of the seed
coating composition before being applied to a seed, such as when being stored.
Examples of suitable biocides include MIT (2-methyl-4-isothiazolin-3-one; CAS No.
10 2682-20-4), BIT (1,2-benzisothiazolin-3-one; CAS No. 2632-33-5) ), CIT (5-Chloro-2
methyl-4-isothiazolin-3-one), Bronopol (2-Bromo-2-nitro-propane-1,3-diol ) and/or a
combination of these.
In an embodiment, the seed coating composition further comprises flakes of
a translucent polymeric film on an inert carrier (a carrier which has no detectable,
15 harmful consequences for the environment, in particular for the seed or the outgrowing
plant in the quantities present) for providing the seeds with a light-reflecting
appearance, such as described in WO-A-03/003812. Preferably, the translucent
polymeric film comprises light-reflecting particles.
The seed coating composition further often comprises one or more solvents.
20 Solvents may be selected from the group consisting of water, alcohols, and
hydrocarbons.Also mixtures of solvents can be used. It is preferred that the solvent is
liquid at 20 °C and 1 atm. Examples of suitable solvents include glycols and their esters
and ethers, in particular ethylene and propylene glycols and their esters and ethers, for
instance, esters and ethers with C 1-C 6 alkyl groups and/or aromatic groups, such as
25 methyl, ethyl, propyl, butyl, benzyl and phenyl ethers, including mono ethers and
dialkyl ethers, and esters of these ethers, such as acetates, and ethylene and
propylene glycol esters, for instance of fatty acids; polyethylene glycol (PEG) and
polypropylene glycol and esters thereof, especially with fatty acids; butyl cellosolve,
butyl carbitol, polyethylene glycol; N-methylpyrrolidone, glycerine, alkyl alcohols with
30 up to 10 carbon atoms, such as ethanol, propanol and butanol. Other examples of
solvents include dipropylene glycol methyl ether and propylene glycol methyl ether. An
important solvent is ethylene glycol. Further examples include propylene tetramer and
synthetic ester oils such as lactate esters, particularly ethyl lactate and benzoate esters
e.g. iso-propyl or 2-ethylhexyl benzoates. Aromatic hydrocarbons such as xylene,
35 aliphatic and paraffinic solvents and vegetable oils can also be used as solvent.
Aromatic solvents are less preferred.
WO 2017/081535 PCT/IB2016/001661
10

The seed coating composition may also comprise components with a


plasticising effect, such as surfactants or antifreeze agents. Common surfactants
include amphiphilic organic compounds, usually comprising a branched, linear or
aromatic hydrocarbon, fluorocarbon or siloxane chain as tail and a hydrophilic group.
5 Some types of surfactants include non-ionic, anionic, cationic and amphoteric
surfactants, and organosilicone and organofluorine surfactants. Some examples of
surfactants include polyoxyethylene glycol and polyoxypropylene ethers and esters, in
particular alkyl, aryl and alkylaryl ethers thereof, and sulphates, phosphates and
sulphonic acid compounds of such ethers, glucoside (alkyl) ethers, glycerol esters, such
10 as alkyl and fatty acid esters, sorbitan (alkyl) esters, acetylene compounds, cocamide
compounds, block copolymers of polyethylene glycol and propylene glycol. Further
examples of surfactants include alkylamine salts and alkyl quaternary ammonium salts,
for example betain type surfactants, amino acid type surfactants; and polyhedric
alcohols, fatty acid esters, in particular C12 -C 18 fatty acids, for instance of polyglycerin,
15 pentaerythritol, sorbitol, sorbitan, and sucrose, polyhydric alcohol alkyl ethers, fatty
acid alkanol amides, and propoxylated and ethoxylated compounds such as fatty
alcohol ethoxylates, polyethyxlated tallow amine and alkylphenol ethoxylates. Some
examples of anionic surfactants include carboxylic acids, copolymers of carboxylic
acids, sulphates, sulphonic acid compounds and phosphates, for example lignin
20 sulphonates and (linear) alkylaryl sulphonates.
Anti-freeze agents include for example: ethylene glycol, propylene glycol,
1,3-butylene glycol, hexylene glycol, diethylene glycol, and glycerin, with the preferred
glycol being ethylene glycol and propylene glycol.
The seed coating composition of the invention may be applied to the seed in
25 conventional manners.
In an embodiment, the seed is not yet provided with artificial layers prior to
applying the seed coating composition, for example layers comprising a binder, such as
a polymer. Accordingly, the seed coating composition is preferably applied directly on
the natural outer surface of the seed. Nonetheless, it is possible that the seed surface
30 has undergone a surface treatment prior to applying the seed coating composition. It is
possible that such surface treatment does not entail the provision of an artificial layer,
but involves a physical change or modification of the surface of part of the seed or the
entire surface of the seed. For example, the surface treatment may involve increasing
the surface roughness of the seed, such as by selective removal of parts of the seed
35 coat, selective deformation of the seed coat, or a combination thereof. Typically, the
treatment may involve introducing micro roughness on the seed surface. It is also
possible that the seed surface is subjected to a surface treatment that does involve the
WO 2017/081535 PCT/IB2016/001661
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provision of an artificial layer, such as the application of a primer coating layer on the
seed surface. Other suitable surface treatments include, e.g., plasma surface
treatment, contacting the seed with an abrasive material, exposure to hot and humid
air, flame treatment, laser treatment, and electron beam surface treatment.
5 Preferably, the coating composition is applied as a liquid composition and/or
latex composition and thereafter solidified (including cured and/or dried) to form a seed
coating. The term "liquid coating composition" as used in this application is meant to
include coating compositions in the form of a suspension, solution, emulsion, or
dispersion, preferably an emulsion or dispersion.
10 Conventional means of coating may be employed for coating the seeds.
Various coating machines are available to the person skilled in the art. Some
well-known techniques include the use of drum coaters, fluidised bed techniques, rotary
coaters (with and without integrated drying), and spouted beds. Suitably, the seed
coating composition is applied to the seed by a rotary coater, a rotary dry coater, a pan
15 coater or a continuous treater.
Typically, the amount of seed coating composition applied to the seed can
be in the range of 0.5-50 g per kg seed, such as 1-40 g per kg seed, 2-35 g per kg
seed, or 3-30 g per kg seed.
The seed coating composition can, for instance, be applied by film coating,
20 spraying, dipping, or brushing of the seed coating composition. Optionally, it is applied
at a temperature of -25 °C to 50 °C, for instance -5 °C to 35 °C, more often 15 °C to
30 °C, for instance at room temperature, such as 18 °C to 25 °C. Preferably, the seed
coating composition is applied to the seed by film coating. The film coating may
suitably be applied by spraying the liquid coating composition onto the seed, typically
25 while the seeds fall or flow through a coating apparatus. Preferably, the method
comprises film coating of the seed to apply the seed coating composition in the form of
a film coating composition.
Seed coating typically involves forming on the surface of the seeds a firmly
adhering, moisture-permeable coating. The process typically comprises applying a
30 liquid seed coating composition to the seeds before planting.
Examples
Example 1
Film coating formulations were prepared according to table 1. A PPP (plant
protection products) cocktail was used consisting of 52.7 wt.% hydrophobic insecticide
35 and 47.3 wt.% fungicide. Soybean seeds were coated with a slurry of 43.1 wt.% PPP
cocktail, 43.3 wt.% film coating formulations and 13.6 wt.% Color Coat Red (pigment
concentrate from BASF); the application rate being 5.5 g/kg seeds, such that 2.38 g
WO 2017/081535 PCT/IB2016/001661
12

film coating formulation per kg seed was applied. A reference sample was prepared by
coating soybean seeds with a slurry consisting of 43.1 wt.% PPP cocktail, 43.3 wt.%
water and 13.6 wt.% Color Coat Red.
Table 1: Composition of film-coat formulations.
Film-Coat A Film-Coat B
(%t/wt) (%t/wt)
wax emulsion
water 60 (42o%) 78.59
rheology additive 0.2 defoamer 0.01
defoamer 0.1 additive 1
biocide 0.2 biocide 0.2
vinyl acetate binder
emulsion (50 %) 25 rheology additive 0.2
white pigment
concentrate 5 effect pigment 20
red pigment
concentrate 9.5

5
Film-Coat C
(%wt/wt)
water 50.82
starch 6.0
defoamer 0.05
additive 1.20
effect pigment 16.73
biocide 0.20
wax emulsion (42 %) 25

The release of the hydrophobic insecticide in water from coated soybean seeds
was measured according to the following protocol. 6 treated soybean seeds were
placed in a plastic centrifuge tube with 20 ml of deionised water. After one hour, the
10 water was decanted off the seeds, sampled for LCMS analyses, and replaced by 20 ml
of clean deionised water in the centrifuge tube containing the seeds. The same
procedure was repeated after 2, 3, 4, 24 and 48 hours. Upon collecting the 48 hour
water sample, the seeds were immersed into 10 ml of acetonitrile (AcN) to extract any
residual active ingredient, all solvents were evaporated. Liquid Chromatrography Mass
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13

Spectrometry (LCMS) analyses were performed on the 1, 2, 3, 4, 24, 48 hours and AcN
extraction samples. Samples were prepared by adding 2 ml of acetonitrile (containing
0.1 vol.% of trifluoroacetic acid) to the vials and shaking them at 40 OC for 30 minutes.
Subsequently, 2 ml of deionised water (containing 0.1 vol.% of trifluoroacetic acid)
5 were added, resulting in a total sample volume of 4 ml. These samples were shaken at
40 0 C for 30 more minutes, after which they were filtered through a 0.2 microns filter.
Figure 1 shows the release patterns of the hydrophobic insecticide in water
measured on coated soybean seeds. The release patterns of hydrophobic insecticide in
water represented in figure 1 show that the reference soybean seeds sample coated
10 without film-coat liquid displayed the fastest release profile. Using a standard film-coat
formulation such as Film-Coat A, based on a polyvinyl acetate binder, resulted in no
release of the active ingredient. Film-Coat B formulation, based a high content of wax,
resulted in a fast release of the active in water and low residual hydrophobic insecticide
on the seed at the end of the release test.
15 Example 2
Film coating formulations were prepared according to tables 2 and 3. A PPP
cocktail was used consisting of 45.2 wt.% hydrophobic insecticide, 47.3 wt.%
insecticide and 7.5 wt.% fungicide. Corn seeds were coated with a slurry of 46.3 wt.%
PPP cocktail, 2.7 wt.% Color Coat Red (pigment concentrate obtained from BASF), 19.4
20 wt.% film coating formulations, and 31.6 wt.% water; the application rate being 16.5
g/kg seeds, such that 3.20 g film coating formulation per kg seed was applied.
Table 2: Composition of film-coat formulations.
Film-Coat D Film-Coat E
(O/owt/wt) (O/owt/wt)
water 18.25 water 47.21
vinyl acetate binder
rheology additive 13.27 emulsion (50 %) 2
styrene acrylate binder
emulsion (500%) 25.9 rheology additive 0.3
defoamer 0.05 defoamer 0.1
additive 0.6 biocide 0.3
effect pigment 16.73 biocide 0.09
biocide 0.2 effect pigment 20
wax emulsion (42
wax emulsion (42%) 25 %) 30
WO 2017/081535 PCT/IB2016/001661
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Table 3: Composition of film-coat formulations.


Film-Coat F Film-Coat G
(%wt/wt) (%wt/wt)
wax emulsion
wax emulsion (42 %) 74.0 (42%) 78.39
defoamer 0.05 defoamer 0.01
additive 0.75 additive 1.0
biocide 0.2 biocide 0.2
effect pigment 25 rheology additive 0.4
effect pigment 20

The release patterns of the hydrophobic insecticide in water from treated


corn seeds were characterized following the same protocol as described in example 1,
5 employing 6 coated corn seeds per test.
Figure 2 shows release patterns of hydrophobic insecticide in water
measured on coated corn seeds. The hydrophobic insecticide release patterns in water
from coated corn seeds displayed in figure 2 show again that the slurry treatment
performed without film-coat formulation enabled a high release rate of the active in
10 waters. When employing a film-coat formulation such as Film-Coat D, based on a
styrene acrylate binder, the release was considerably slowed down. Film-Coat E, based
on a lower binder/wax ratio, accelerated the release of the active in water, while Film
Coat B, based on a high wax content and no binder, enabled to obtain even higher
release rates and residual amounts of active on the seeds similar to those obtained in
15 the absence of film-coat formulation.
Example 3
Soybean bioassays were performed by planting 12 seeds per treatment in a
soil mixture composed of 51 % silt, 29 % clay, and 20 % sand. Plants were grown at
about 20-25 °C, exposed to 12 hours of light per day, watered daily, and fertilised once
20 per week. At each relevant trifoliate (1st through 4th), 8 total leaves were clipped for
each treatment type at the base of the leaf, taking only one leaf per plant. Figure 3
shows the sampling procedure for 1 st trifoliate soybean leaves for a bioassay. Leaves
were bioassayed by placing one soybean looper (Pseudoplusia includens) on one
soybean leaf measuring 22 mm in diameter in a sealed tray at 25 °C, where it was
25 allowed to feed on leaf material for four days. At that time leaf consumption and insect
WO 2017/081535 PCT/IB2016/001661
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mortality was noted for each sample, where each data point is an average of eight
leaves of the same treatment.
Figure 4 shows a comparison of % leaf consumption of the soybean leaf
discs of various film-coats to that of untreated seeds and seeds treated with PPPs only.
5 The positive control of untreated seeds show high leaf consumption and poor insect
control, while the negative control of seeds treated with PPPs only show low leaf
consumption and better insect control. Mirroring the release data, Film-Coat A shows
relatively high leaf consumption, indicating that it is unable to successfully uptake the
active into the plant and protect from insecticidal activity. Film-coats containing a wax
10 (B and F), and also containing starch as a binder (C) all show low leaf consumption
similar to that of the PPP alone seeds. Film-Coat B is clearly the most effective at
uptaking the actives and therefore controlling the insects, keeping leaf consumption
under 10 % up to 4th trifoliate.
Example 4
15 Corn bioassays were performed by planting 4 seeds per treatment in a soil
mixture composed of 51 / silt, 29 / clay, and 20 % sand. Plants were grown at about
20-25 °C, exposed to 12 hours of light per day and watered daily. Typical period of
plant growth consisted of three weeks from sowing to clipping of the leaves for
bioassay. The leaves 3 rd and 4th were clipped at the growth stage wherein, the fifth leaf
20 was still in the whorl. The leaves were numbered as shown in the figure 5. The 3 rd leaf
was clipped at 3 cm from the base and further divided into two sections (Sample 1 & 2)
for bioassay. The 4th leaf was clipped at the base and further sectioned into two parts
as shown in the figure 5. For each treatment, leaves from three plants were collected
totaling 12 samples per treatment. Each of the samples (4.5 cm section) were then
25 placed in separate bioassay trays and one 2 nd instar larvae of fall armyworm
(Spodoptera frugiperda) was introduced to each tray. The trays were then sealed and
placed at 25 °C for four days. After four days, the / leaf consumption for each
treatment (12 samples) were averaged to compared the efficacy of the across various
treatment.
30 Figure 6 shows the 0/ leaf consumption of the leaf tissue from corn treated
with different film-coats. The lower the leaf consumption, higher is the efficacy of the
coating in releasing the actives thereby leading to better insect control. Leaf tissue
from Untreated Corn seeds was tested as the negative control resulting in very high
leaf consumption. The actives without any film-coat was tested as the positive control
35 owing to the fact that having no film-coat could effortlessly release the actives and
improve uptake by the plant. Of the film-coats tested, Film-Coat B provided superior
bioefficacy comparable to the positive control followed by Film-Coat G. The high wax
WO 2017/081535 PCT/IB2016/001661
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content in these film-coats provided faster release of actives from the coating and
resulted in better uptake by plants. In addition, to high wax based coating, coatings
based on water soluble binder Film-Coat C also showed leaf consumption below 10

%
owing to better solubility of Film-Coat C in water resulting in faster release of actives.
5 Example 5
In addition to facilitating the uptake of actives, the new film coats are also
able to reduce the amount of dust-off produced by soybeans. 100 grams of seeds were
submitted to a 2-minute Heubach test in duplicate, averaging the results to a total
amount of dust-off per 100 000 seeds (figure 7). In the case of soybeans, untreated
10 seeds give off about 0.14897 g of dust per 100 000 seeds, which can be reduced about
4-fold when the seeds are treated with PPPs alone or traditional Film-Coat A. Use of the
wax-based Film-Coat B and Film-Coat F, however, result in a further 4.5 and 2 fold
decrease in dust, respectively, from that of PPP alone treated seeds. Starch-based
Film-Coat C also allows for a 1.5 fold decrease in dust over the PPP alone treated
15 seeds.
Example 6
If not carefully treated, seeds coated with actives and other enhancements
result in harmful levels of dust during handling and processing of huge quantities of
seeds. One of the primary objectives of a film-coating is to retain the actives on the
20 seed and reduce dust while in operation. In addition to improving bioefficacy, the film
coats were also tested for dust reduction and abrasion resistance. Dust and abrasion
data for corn treated with film-coats were obtained by following industry standards.
Specific amount of seeds were placed in the Heubach apparatus and tested for 4
minutes. The dust collected on the filter paper was gravimetrically measured to
25 evaluate different film-coat formulation.
Figure 8 shows the dust (g / 100 000 seeds) for the different film-coat
formulations tested on corn. Film-Coat B showed about 75 % lower dust when
compared to PPP alone. Film-Coat D showed similar performance, which was expected
as it contains an acrylate-based binder system that is well-known for containing dust
30 levels. Film-Coats E, F and G are all based on high wax content and showed statistically
similar dust levels reducing dust about 60-65 % than PPP alone.
Abrasion on the corn seed was visually observed after the dust experiment
in the Heubach apparatus. The abrasion score is a visual quantification of the quality of
seeds after subjecting them to 4 minutes in the Heubach apparatus closely simulating
35 handling conditions in the industry. The abrasion score was allocated from 1 (high
abrasion resistance/good quality seeds) to 5 (low abrasion resistance/poor quality
seeds). All of the film-coat formulations showed abrasion score of 2-2.5, while seeds
WO 2017/081535 PCT/IB2016/001661
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with only PPP/no film-coat showed high abrasion and poor quality seeds. Figure 9
shows corn abrasion scores determined after 4 min Heubach tests (1: high abrasion
resistance; 5: poor abrasion resistance).
Example 7
5 Rate of drying of seeds is another important feature that is considered when
choosing film-coat. The rate of drying is checked by using a cotton indicator. The
coated seeds, after exiting the coater is collected in a flat tray and a timer is activated.
The drying is checked every 5-10 s by placing a fresh cotton indicator at a new seeds'
surface in the seed tray each time. The moment there is no sign of colour transfer on
10 the cotton indicator from the seed, the timer is stopped and the time is recorded
Clumping/Bridging of seeds takes place when wet seeds exiting out of the
coater are collected in the storage hopper and compacted by oncoming seeds. This
presents a challenge to the seed treatment facility in terms of equipment blocking,
labor and time. The film-coats described in this embodiment show faster drying times
15 and minimal to no clumping.
New film-coats minimise dry time, tack, and clumping on soybeans. Use of
traditional Film-Coat A and PPP alone treated seeds result in longer dry time, noticeable
tack upon exiting the coater, and clumping after being allowed to sit under 1 kg of
weight for 5 minutes. The new film-coats allow for a noticeably faster dry time,
20 decrease in tackiness upon exiting the coater, and an elimination of clumping all
together (table 4).
Table 4: Comparison of dry time, tack and clumping of film-coats on soybeans.
Dry to touch
Tack Clumping
(seconds)
PPPs only 48 Yes Minimal
Film-Coat A 49 Yes Yes
Film-Coat B 27 Minimal None
None,but
Film-Coat C 38 Yes tacky
Film-Coat F 29 Minimal None

Dry flow of soybeans was measured as the time it took for 1 kg of seeds to
25 flow through a funnel. Typically, the addition of PPPs and traditional film-coats to
soybeans slows down the flow of seeds considerably (figure 10), which is not a desired
characteristic. In fact, the addition of Film-Coat A to soybean seeds decreased flow
more than a full second compared to untreated seeds. New Film-Coats B, C, and F are
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able to effectively decrease flow time considerably, allowing flow almost as fast as
untreated seeds.
Example 8
Rate of drying of seeds is another important feature that is considered when
5 choosing film-coat. The rate of drying is checked by using a cotton indicator. The
coated seeds, after exiting the coater is collected in a flat tray and a timer is activated.
The drying is checked every 5-10 s by placing a fresh cotton indicator at a new seeds
surface in the seed tray each time. The moment there is no sign of colour transfer on
the cotton indicator from the seed, the timer is stopped and the time is recorded.
10 Clumping/Bridging of seeds takes place when wet seeds exiting out of the
coater are collected in the storage hopper and compacted by oncoming seeds. This
presents a challenge to the seed treatment facility in terms of equipment blocking,
labor and time. The film-coats described in this embodiment show faster drying times
and minimal to no clumping.
15 Table 5: Comparison of dry time, tack and clumping of film-coats on corn.
Dry to touch
(seconds)
Only PPP/No
film-coat 24.25 Yes
Film-Coat D 14.4 Yes
Film-Coat B 5.01 None
Film-Coat C 9 None
Film-Coat E 12 Minimal
Film-Coat F 13.53 Minimal
Film-Coat G 24.07 None

The flow of treated/coated seeds is important at the seed treating facility as


well as at the farm whilst going through the planter. Lower the friction between the
seeds, better is the efficiency at various stages. Flow is typically improved by
20 incorporating a flow agent or a slip agent into the fim-coat formulation. A flow agent is
typically a wax-based additive that lowers friction and improves the appearance of the
seed. For the testing the flow of treated seeds, 1 kg of seeds are placed in a funnel
fitted with a stopper. The stopper is opened and timer started simultaneously. The time
taken for the last seed to exit the funnel is recorded as the flow rate in (s/kg). The
25 results are shown in figure 11 and illustrate the improved flow properties of the coated
seeds according to the invention.
19

The claims defining the invention are as follows:

1. A seed coating composition comprising one or more biologically active


ingredients and a wax and/or polymeric carbohydrate, wherein said seed coating
5 composition is an aqueous seed coating composition and has an amount of
polymeric binder excluding wax and polymeric carbohydrate of 4 % or less by
total weight of the wax and polymeric carbohydrate together, wherein:
the wax is in the form of an emulsion;
the wax is selected from the group consisting of polyethylene wax,
10 Fischer-Tropsch wax, and carnauba wax; and
the polymeric carbohydrate is selected from starch, cellulose, and dextrins;
and
said seed coating composition comprises a total of wax and polymeric
carbohydrate in an amount of 20-40 % by total weight of the seed coating
15 composition.

2. The seed coating composition according to claim 1, wherein said wax is anionic
or non-ionic.

20 3. The seed coating composition according to claim 1 or claim 2, wherein said seed
coating composition has an amount of polymeric binder excluding wax and
polymeric carbohydrate of 2 % or less by total weight of the wax and polymeric
carbohydrate together, or 1 % or less.

25 4. The seed coating composition according to any one of claims 1 to 3, wherein


said seed coating composition is essentially free from polymer binder excluding
wax and polymeric carbohydrate.

5. The seed coating composition according to any one of claims 1 to 4, wherein


30 said active ingredients comprise at least one hydrophobic and/or water insoluble
active ingredient.

6. A method for improving the bio-efficacy and/or systemic uptake by plants of one
or more active ingredients in a seed coating composition, comprising preparing
35 a seed coating composition according to any one of claims 1 to 5 and applying
said seed coating composition to seed.

7. A coated seed, comprising a seed coating composition according to any one of


claims 1 to 5.
20

8. The seed coating composition according to any one of claims 1 to 5, that


accelerates drying by an additional 15-25 % based on water content thereby
significantly reducing bridging/clumping of coated seeds.
5
9. The seed coating composition according to any one of claims 1 to 5, that
significantly reduces the coefficient of friction between coated seeds and
improves flowability during processing.

10 10. Use of a seed coating composition comprising one or more biologically active
ingredients and a wax and/or polymeric carbohydrate, wherein said seed coating
composition has an amount of polymeric binder excluding wax and polymeric
carbohydrate of 10 % or less by total weight of the wax and polymeric
carbohydrate together, to reduce the bridging/clumping of the coated seeds
15 and/or reduce the coefficient of friction between the coated seeds and improve
flowability during processing.

11. The use according to claim 10, wherein the coating composition comprises at
least one hydrophobic and/or water insoluble active ingredient.

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