Notes Flamephotometry

FLAME PHOTOMETRY
BASIC CONCEPTS,
INSTRUMENTATION, AND
APPLICATION
CHEM 4138
1
INTRODUCTION:
• Flame photometry (more accurately called Flame Atomic
Emission Spectrometry)is a branch of spectroscopy in which
the species examined in the spectrometer are in the form of
atoms
• A photoelectric flame photometer is an instrument used in
inorganic chemical analysis to determine the concentration
of certain metal ions among them sodium, potassium,
calcium and lithium.
• Flame Photometry is based on measurement of intensity of
the light emitted when a metal is introduced into flame.
– The wavelength of colour tells what the element is (qualitative)
– The colour's intensity tells us how much of the element present
(quantitative)
2
• The basic principle upon which Atomic Spectroscopy
works is based on the fact that "Matter absorbs light
at the same wavelength at which it emits light".
• Atoms of elements  subjected to hot flame 
specific quantum of thermal energy absorbed by
orbital electrons  become unstable at high energy
level  release energy as photons of particular
wavelength  change back to ground state.
• When a metal salt solution is burned, the metal
provides a colored flame and each metal ion gives a
different colored flame.
• Flame tests, therefore, can be used to test for the
absence or presence of a metal ion
3
BASIC CONCEPT:
• Liquid sample contaning metal salt
solution is introduced into a flame,
• Solvent is first vaporized, leaving
particles of solid salt which is then
vaporised into gaseous state
• Gaseous molecule dissociate to
give neutral atoms which can be
excited (made unstable) by thermal
energy of flame
• The unstable excited atoms emit
photons while returning to lower
energy state
• The measurement of emitted
photons forms the basis of flame
photometry. 4
• Under constant and controlled conditions, the light
intensity of the characteristic wavelength produced by
each of the atoms is directly proportional to the
number of atoms that are emitting energy, which in
turn is directly proportional to the concentration of
the substance of interest in the sample.
• Various metals emit a characteristic colour of light
when heated.
5
Structure of Flame:
As seen in the figure, the
flame may be divided into
the following regions or
zones.
– Preheating zones
– Primary reaction zone or
inner zone
– Interconal zone
– Secondary reaction zone
6
• preheating zone- In this, combustion mixture is
heated to the ignition temperature by thermal
conduction from the primary reaction zone.
• primary reaction zone- This zone is about 0.1 mm
thick at atmospheric pressure
– There is no thermodynamic equilibrium in this zone and the
concentration of ions and free radicals is very high.
– This region is not used for flame photometry.
• interconal zone – It can extend up to considerable
height. The maximum temperature is achieved just
above the tip of the inner zone.
– This zone is used for flame photometry.
• secondary reaction zone - In this zone, the products of
the combustion processes are burnt to stable
molecular species by the surrounding air. 7
Flame Types and Temperatures
Fuel Oxidant Temperature 0C

Natural gas Air 1700-1900
Natural gas Oxygen 2700-2800
Hydrogen Air 2000-2100
Hydrogen Oxygen 2550-2700
Acetylene Air 2100-2400
Acetylene Oxygen 3050-3150
Acetylene Nitrous oxide 2600-2800
8
INSTRUMENTATION:
THE FLAME PHOTOMETER

Schematic Representation of the Flame Photometer
Major Components:
1. Sample Delivery
System
2. Source
3. Monochromator
4. Detector
5. Read out device
10
Sample Delivery System:
There are three components for introducing liquid

sample:
• Nebulizer – it breaks up the liquid into small droplets.
– Nebulization is the conversion of a sample to a mist of finely
divided droplets using a jet of compressed gas.
– The flow carries the sample into the atomization region.
– Pneumatic Nebulizers: (most common)
• Aerosol modifier – it removes large droplets from the
stream and allow only smaller droplets than a certain size
to pass
• Flame or Atomizer – it converts the analyte into free
atoms 11
Source:
• A Burner used to spray the sample solution into fine

droplets.
• Several burners and fuel + oxidant combinations have
been used to produce analytical flame including:
Premixed, Mecker, Total consumption, Lundergarh,
Shielded burner, and Nitrous oxide-acetylene flames
• Pre-mixed Burner:
– widely used because uniformity in flame intensity
– In this energy type of burner , aspirated sample , fuel
and oxidant are thoroughly mixed before reaching the
burner opening. 12
• Total Consumption Burner:
– In this fuel and oxidant are hydrogen and oxygen gases

– Sample solution is aspirated through a capillary by high
pressure of fuel and Oxidant and burnt at the tip of burner
– Entire sample is consumed.
Pre-mixed burner Total Consumption Burner

13
Monochromator:
– Prism: Quartz material is used for making prism, as quartz is
transparent over entire region
– Grating: it employs a grating which is essentially a series of
parallel straight lines cut into a plane surface
Detectors:
– Photomultiplier tubes
– Photo emissive cell
– Photo voltaic cell
Photovoltaic cell:
• It has a thin metallic layer coated with silver or gold which
act as electrode, also has metal base plate which act as
another electrode
• Two layers are separated by semiconductor layer of
selenium, when light radiation falls on selenium layer.
• This creates potential diff. between the two electrode and
cause flow of current. 14
Read-out Device:
• It is capable of displaying the absorption spectrum as well
absorbance at specific wavelength
• Nowadays the instruments have microprocessor controlled
electronics that provides outputs compatible with the
printers and computers
• Thereby minimizing the possibility of operator error in
transferring data.
Element wavelength Detection Element wavelength Detection

limit limit
Al 396 0.5 Pb 406 14
Ba 455 3 Li 461 0.067
Ca 423 0.07 Mg 285 1
Cu 325 0.6 Ni 355 1.6
Fe 372 2.5 Hg 254 2.5
Elements, their characteristic emission wavelengths and detection limits
15
INTERFERENCES:
• In determining the amount of a particular element
present, other elements can also affect the result.
Such interference may be of 3 kinds:
• Spectral interferences: occurs when the emission lines
of two elements cannot be resolved or arises from the
background of flame itself.
– They are either too close, or overlap, or occur due to high
concentration of salts in the sample
• Ionic interferences: high temperature flame may cause
ionisation of some of the metal atoms, e.g. sodium.
– The Na+ ion possesses an emission spectrum of its own
with frequencies, which are different from those of atomic
spectrum of the Na atom. 16
• Chemical interferences: The chemical interferences
arise out of the reaction between different
interferents and the analyte. Includes:
• Cation-anaion interference:
– The presence of certain anions, such as oxalate,
phosphate, sulfate, in a solution may affect the
intensity of radiation emitted by an element. E.g.,
– calcium + phosphate ion forms a stable substance, as
Ca3(PO4)2 which does not decompose easily, resulting in
the production of lesser atoms.
• Cation-cation interference:
– These interferences are neither spectral nor ionic in
nature
– E.g. aluminum interferes with calcium and magnesium.
17
APPLICATIONS:
• To estimate sodium, potassium, calcium, lithium etc.
level in sample of serum, urine, CSF and other body
fluids.
• Flame photometry is useful for the determination of
alkali and alkaline earth metals.
• Used in determination of lead in petrol.
• Used in the study of equilibrium constants involving
in ion exchange resins.
• Used in determination of calcium and magnesium in
cement.
18
THANK YOU
19

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FLAME PHOTOMETRY

Fuel Oxidant Temperature 0C

THE FLAME PHOTOMETER

There are three components for introducing liquid

• A Burner used to spray the sample solution into fine

– In this fuel and oxidant are hydrogen and oxygen gases

Pre-mixed burner Total Consumption Burner

Element wavelength Detection Element wavelength Detection

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