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    Chapter 6

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    voquocdangkhoa2003
    Here are the steps to solve this problem: 1) Set up two equations with the two absorbance values and the molar absorptivities 2) Solve the two equations simultaneously to find [Cr3+] and [Co2+] Report the concentrations of Cr3+ and Co2+ in the mixture.

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    Chapter 6

    Uploaded by

    voquocdangkhoa2003
    18 views15 pages
    Here are the steps to solve this problem: 1) Set up two equations with the two absorbance values and the molar absorptivities 2) Solve the two equations simultaneously to find [Cr3+] and [Co2+] Report the concentrations of Cr3+ and Co2+ in the mixture.

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    Chapter-6

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    Here are the steps to solve this problem: 1) Set up two equations with the two absorbance values and the molar absorptivities 2) Solve the two equations simultaneously to find [Cr3+] and [Co2+] Report the concentrations of Cr3+ and Co2+ in the mixture.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    18 views15 pages

    Chapter 6

    Uploaded by

    voquocdangkhoa2003
    Here are the steps to solve this problem: 1) Set up two equations with the two absorbance values and the molar absorptivities 2) Solve the two equations simultaneously to find [Cr3+] and [Co2+] Report the concentrations of Cr3+ and Co2+ in the mixture.

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    Chapter 6

    Spectroscopic method-
    UV/Vis
    Energy diagram
    Components of Spectrophotometer
    Sample container (Cuvette)
     For Visible and UV spectroscopy, a liquid sample is usually
    contained in a cell called a cuvette.

     Glass is suitable for visible but not for UV spectroscopy because it


    absorbs UV radiation.

     Quartz can be used in UV as well as in visible


    spectroscopy Long 1 cm pathlength cu
    pathlength
    Opaque
    Face

    Transparent
    Face

    1 cm 1 cm
    Short
    Holding capacity 1.5 mL to 3.5 mL
    pathlength (b)
    Absorbance Spectra

    Visible absorbance spectrum for cranberry juice


    (anthocyanin dyes )
    Transmittance and Absorbance
    Principles
    Beer’s Law
    The amount of light absorbed is proportional
    to the concentration of the absorbing
    substance

    100% light 40% light 100% light 70% light

    60% light absorbed 30% light absorbed


    Rate of Absorption
    Lambert ’s Law

    The amount of light absorbed is proportional to the


    thickness (length) of the absorbing material
    (Cuvette)

    100% light 40% light 100% light 70% light

    Larger path length


    Smaller path length

    60% light absorbed 30% light absorbed

    Rate of Absorption
    Absorbance and Concentration:
    Lambert-Beer’s Law

    a is the analyte’s absorptivity [cm–1.conc–1]


    If the concentration is expressed in molarity:
     a: molar absorptivity,  , [cm–1.M–1), the Beer’s Law is
    rewritten:
    Lambert-Beer’s Law and
    Multicomponent Samples

    For a two-component mixture of analyte’s X and Y, the total


    absorbance, Atot, is

    Generalizing, the absorbance for a mixture of n components,


    Amix, is:
    Applications
     The applications of UV/Vis Spectrometer are quite
    vast.
     In analytical chemistry: quantitative determination
    of different analytes, such as metal ions,
    highly conjugated organic compounds, and
    biological macromolecules.
     Spectroscopic analysis is commonly carried out in
    solutions but solids and gases may also be studied.
    *** if an analyte does not absorb UV/Vis radiation—
    (or too weak): react it with another species that is
    strongly absorbing (EX: Fe2+ with o-phenanthroline)
    Q&A
    Q&A

    The concentration of Cu2+ in a sample is determined by reacting


    it with the ligand cuprizone and measuring its absorbance at 606
    nm in a 1.00-cm cell. When a 5.00-mL sample is treated with
    cuprizone and diluted to 10.00 mL, the resulting solution has an
    absorbance of 0.118. A second 5.00-mL sample is mixed with
    1.00 mL of a 20.00 mg/L standard of Cu2+, treated with cuprizone
    and diluted to 10.00 mL, giving an absorbance of 0.162. Report
    the mg Cu2+/L in the sample. (5.4)
    Q&A

    The absorbance spectra for Cr3+ and Co2+ overlap significantly.


    To determine the concentration of these analytes in a mixture, its
    absorbance is measured at 400 nm and at 505 nm, yielding
    values of 0.336 and 0.187, respectively. The individual molar
    absorptivities (M–1.cm–1) for Cr3+ are 15.2 at 400 nm and 0.533 at
    505 nm; the values for Co2+ are 5.60 at 400 nm and 5.07 at 505
    nm.

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