5991-8450EN Chiral Columns Appcompendium Compressed

Put InfinityLab Poroshell 120
Chiral innovation to work for

your challenging separations
Application Compendium
Perform fast, high-efficiency chiral
separations like never before
Now you don’t have to compromise on your chiral
separations. InfinityLab Poroshell 120 Chiral columns are
the first columns to combine superficially porous particles
with innovative chiral stationary phases to deliver:
–– H
igher performance and speed, compared to totally
porous chiral stationary phases
–– Ruggedness and reliability with proven Agilent InfinityLab
Poroshell 120 particle technology
–– Sizes to suit any application: 2.1 and 4.6 mm ID
configurations in 50, 100 and 150 mm lengths
–– Fast run times, superior peak shape, and high resolution
–– Increased throughput and lab productivity with more
efficient chiral separations
1 2
Perform chiral separations in less than 5 minutes using your existing LC systems 3
4
Superficially porous particles provide higher efficiency and sharper peak shapes. Throughput is dramatically improved with
shorter runtimes, compared to fully porous particle columns.
0 5 10 15 min
Traditional Chiral Separation— Agilent InfinityLab Poroshell 120 Chiral Separation—

totally porous particle superficially porous particle
Chirobiotic V2 (250 x 4.6 mm, 5 µm) InfinityLab Poroshell 120 Chiral-V (100 x 4.6 mm, 2.7 µm)
1
1 23
2 3
Separation of amphetamines
4 4
in under 5 minutes
0 5 10 15 min
0 5 10 15 min
1. D-(+)-Amphetamine, 2. L-(-)-Amphetamine, 3. D-(+)-Methamphetamine 1. D-(+)-Amphetamine, 2. L-(-)-Amphetamine, 3. D-(+)-Methamphetamine

4. L-(-)-Methamphetamine 100/0.1/0.02 MeOH/HOAc/NH4OH with a 4. L-(-)-Methamphetamine 100/0.1/0.02 MeOH/HOAc/NH4OH with a
1.0 mL/min flow rate at room temperature and UV at 220 nm 1.0 mL/min flow rate at room temperature and UV at 220 nm
1
23
4
0 5 10 15 min
2
Column selection guidelines
Separate nearly any chiral compound:
Four chemistries across four different LC modes offer a wide range of selectivities
Column Chemistry Chiral Selector (bonded chemistry) Typical LC Mode Typical Applications
Polar Organic (PO) Primary amines

InfinityLab Poroshell 120 Chiral-CF Derivatized cyclofructan (CF6)
Normal Phase (NP) Primary amines
Reversed Phase (RP) Stimulants, fungicides, t-boc amino acids

InfinityLab Poroshell 120 Chiral-CD Hydroxypropylated-β-cyclodextrin
Polar Organic (PO) Complex molecules
Polar Ionic (PI) Basic pharmaceuticals (various)
InfinityLab Poroshell 120 Chiral-V Vancomycin (macrolide antibiotic) Reversed Phase (RP) Amines, profens
Polar Organic (PO) Complex neutral molecules
Polar Ionic (PI) Beta blockers, hydroxyl acids
InfinityLab Poroshell 120 Chiral-T Teicoplanin (macrolide antibiotic) Reversed Phase (RP) Amino acids, hydroxyl acids, profens
Polar Organic (PO) Hydantoins, benzodiazepines
RP = Reversed phase PI = Polar iconic PO = Polar organic NP = Normal phase
Ordering Information
Part number Column description Part number Column description
689775-609 InfinityLab Poroshell 120 Chiral-CF, 2.1x50 mm, 2.7 µm 689775-604 InfinityLab Poroshell 120 Chiral-V, 2.1x50 mm, 2.7 µm
689775-607 InfinityLab Poroshell 120 Chiral-CD, 2.1x50 mm, 2.7 µm 689775-603 InfinityLab Poroshell 120 Chiral-T, 2.1x50 mm, 2.7 µm
Learn more about detecting enantiomers quickly when time is short.

www.agilent.com/chem/poroshell-120-chiral
3
Use this general protocol to develop robust chiral screening methods
Chiral screening protocol

Typical run conditions (4.6 x 100 mm)
# Mobile Phase Composition (% v) Type
Adjust for 2.1 mm ID (or other dimension) columns
1 MeOH/20 mM ammonium formate, pH 4.0 90/10 RP Flow Rate: 1 mL/min
2 MeOH/20 mM ammonium formate, pH 4.0 30/70 RP

Detector: UV
Equilibration: 10 column volumes
3 ACN/20 mM ammonium formate, pH 4.0 30/70 RP
Run Time: 10 column volumes
4 ACN/20 mM ammonium formate, pH 4.0 10/90 RP Temperature: Ambient
Concentration: 2-5 mg/mL
5 MeOH/ammonium formate 100/0.2wt% PI
Sample in: MeOH or mobile phase
6 ACN/MeOH/Acetic Acid/TEA 60/40/0.3/0.2 PO
7 EtOH/Heptane/Acetic Acid/TEA 20/80/0.3/0.2 NP
RP = Reversed phase PI = Polar iconic PO = Polar organic NP = Normal phase
Priority of mobile phases for screening Reversed phase mode (RP): Typically
ACN or MeOH combined with aqueous
Column Chemistry First choice Second choice Third choice (salt buffer or modifier) mobile phases
InfinityLab Poroshell Chiral-T #5 (PI) #2 (RP) #1 (RP) Polar organic mode (PO): Polar organic
InfinityLab Poroshell Chiral-V #5 (PI) #3 (RP) #1 (RP) mobile phase solvents (acetonitrile,
methanol) with or without additives
InfinityLab Poroshell Chiral-CD #3 (RP) #6 (PO) #4 (RP)
Polar ionic mode (PI): Salt buffers added
InfinityLab Poroshell Chiral-CF #6 (PO) #7 (NP) -
to polar organic mobile phases to improve
ionization or retention of ionizable
compounds
Normal phase mode (NP): Typically non-

polar solvents are the primary solvent
with no aqueous components
4
Pressure, pH and temperature parameters
Column Chemistry Pressure limit (bar) pH range Temperature limit
InfinityLab Poroshell 120 Chiral-CF 400 bar 3-7 45 °C
InfinityLab Poroshell 120 Chiral-CD 400 bar 3-7 45 °C
InfinityLab Poroshell 120 Chiral-V 400 bar 2.5-7 45 °C
InfinityLab Poroshell 120 Chiral-T 400 bar 2.5-7 45 °C
Care should be taken not to exceed pressure limits when switching mobile phase solvents
Agilent Poroshell 120 Chiral

Cyclofructan Bonded Phase Structures
CF-6 Beta-Cyclodextrin
Underivatized-HILIC OH5 Hydroxypropylated - Chiral-CD
Isopropylated-Chiral-CF
InfinityLab Poroshell 120 Chiral-CF InfinityLab Poroshell 120 Chiral-CD
(Cyclofructan CF-6) (Hydroxypropylated beta-cyclodextrin)
HO CH2OH
O
O
OH O O
HO RO CH2OH
O
OHCH2 OR RO
OH OH
O RO O
HO
O
HO O HO OR RO
O
O
O O
OH
OH
O OR RO
O
O
OH CH2OH
O
OH
O
OH OHCH2 OR RO O
O HO RO
HO
O
O OR OR O
OH RO
HO
O CH2OH
O
OH
CH2OH Teicoplanin
O
Vancomycin Chiral-T
Chiral-V
InfinityLab Poroshell 120 Chiral-V InfinityLab Poroshell 120 Chiral-T
(Vancomycin) (Teicoplanin)
OH
H 3C HO
NHR
NH HO
HO OH
NH2 CH2OH O O Cl
Cl
O NH O O O
NH COOH H
O NH CH2OH O O
NH
O O H H O O
HO O NH H H
O O N N NH2
NH HO HNCOCH3 N N N
Cl
O OH H H O H H O H H H
HN H
HO
O HOOC
Cl O
O
HO O HO HO
OH
HO O
O
HO CH3 OH CH2OH
HO O O
O OH
NH2 OH
OH
CH3
5
Bring new drugs to market
more quickly and confidently
Don’t just produce data… find answers

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costly. You can drive greater efficiency and productivity with Agilent integrated
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From automated systems for early-stage compound identification… to
sophisticated, sensitive LC/MS systems for ADME/PK studies… we can
help you produce accurate, reproducible results. So you can make the right
decisions across your pharmaceutical discovery and development pipeline
www.agilent.com/chem/pharma
Agilent InfinityLab: Maximum efficiency for your LC workflow

InfinityLab is an optimized portfolio of LC systems, columns, and supplies
designed to work together seamlessly for maximum efficiency and
performance. From routine analysis to cutting-edge research, InfinityLab gives
you the ability to:
–– Easily identify products that work with your Agilent InfinityLab LC system
–– Maximize your LC workflow with the latest innovations
–– Increase analytical, instrument, and laboratory efficiency
Together with Agilent OpenLAB software and Agilent CrossLab Services,
Agilent InfinityLab provides the end-to-end solutions and support you need
to ensure the best outcomes.
Agilent InfinityLab Supplies: Save time and minimize troubleshooting

InfinityLab Quick Connect fittings
Now you can be sure you’re getting a perfect column connection, from every
user, every time with simple-to-use, finger-tight connections. Quick-Connect
fittings are reusable for more than 200 reconnections and stable up to
1300 bar.
InfinityLab Quick Turn fittings

For instrument connections that are in confined spaces, you can rely on
spring-loaded, Agilent InfinityLab Quick Turn fittings for a perfect connection,
every time.
www.agilent.com/chem/infinitylab
6
Yes, you can shorten run times—
and improve resolution—for Agilent
chiral separations
InfinityLab Poroshell 120 Chiral columns
Like all InfinityLab Poroshell 120 columns, Poroshell

Challenge
120 Chiral columns are based on superficially porous Resolution of chiral enantiomers
particle technology, which features a solid silica core
and a porous outer layer.
R-form S-form R-form S-form
Compared to traditional totally porous particles of
the same (or similar) size, Agilent Poroshell particles
deliver higher chromatographic efficiencies and
enable fast, high-resolution separations.
Before Now
Innovation
Agilent Poroshell superficially porous particle technology
2.7 μm
Speed
10-20 1-3
minutes minutes
2.7 μm 2.7 μm
2.7 μm
Conventional Fast Poroshell
chiral separation chiral separation
Productivity
Conventional column
10x throughput with
Agilent InfinityLab
Poroshell 120
Chiral columns 2.7
2.7
μmμm
7
Table of Contents by Analyte
Amines Analgesics Depressants

1-benzyl-2,2-diphenylethylamine................... 10 Methadone........................................................... 24 Baclofen................................................................ 41
1,1'-binaphthyl-2,2'-diamine............................. 10 Proglumide........................................................... 24 Octoclothepin...................................................... 41
1-(1-naphthyl)ethylamine................................. 11 Indoprofen............................................................ 25 Tolperisone........................................................... 41
1-(1,1-biphenyl-4-yl)ethanamine..................... 11 Ketorolac............................................................... 25
Diuretics
1-(4-methoxyphenyl)ethylamine.................... 12 Methorphan......................................................... 25
Chlorthalidone..................................................... 42
1-aminotetralin.................................................... 12 Nefopam............................................................... 25
Bendroflumethazide.......................................... 42
6-methoxy-1,2,3,4-tetrahydro-1- Tramadol...............................................................26
naphthalenylamine............................................ 13 Fungicides
Anesthetics
1-(2-methoxyphenyl)ethanamine.................. 13 Benalaxyl...............................................................43
Hexobarbital.........................................................27
1-(4-chlorophenyl)ethylamine......................... 13 Econazole.............................................................43
Mepivacaine.........................................................27
1,1-diphenyl-1-fluoro-2-aminopropane......... 14 Flutriafol................................................................43
Bupivicaine...........................................................28
1,1-diphenyl-2-aminopropane......................... 14 Miconazole...........................................................43
Mephobarbital.....................................................28
1,2,2-triphenylethylamine................................. 14 Praziquantel.........................................................44
Prilocaine..............................................................28
2-amino-4-methyl-1,1-diphenylpentane....... 14 Prothioconazole..................................................44
2-chloro-indan-1-ylamine................................. 15 Anticoagulants Dichloroprop........................................................44
4-methoxy-α-methylbenzylamine Coumachlor..........................................................29 Fenoprop...............................................................44
bis(1-phenylethyl)amine................................... 15 Warfarin.................................................................29 Haloxyfop..............................................................45
bis(α-methylbenzyl)amine............................... 15 Mandipropamid...................................................45
Anticonvulsants
Mexiletine.............................................................. 16 Mecoprop methyl ester....................................45
Phensuximide......................................................30
N-benzyl-α-methylbenzylamine.................... 16 Mecoprop..............................................................45
α-methyl-α-phenylsuccinimide......................30
N,α-dimethylbenzylamine................................ 16 Napropamid.........................................................46
α-methyl-4-nitrobenzylamine......................... 16 Antidepressants Bromacil................................................................46
α-methylbenzylamine....................................... 17 Bupropion............................................................. 31
Miscellaneous
Temazepam......................................................... 31
Amino acid & Derivatives 2'-amino-1,1'-binaphthalen-2-ol...................... 47
Lorazepam............................................................ 31
2-amino-2-pheylbutyric acid........................... 18 Hydratropic acid.................................................. 47
Lormetazepam.................................................... 31
4-nitrophenylalanine.......................................... 18 Tropic acid............................................................ 47
Mianserin..............................................................32
AQC Methionine.................................................. 18 Jacobsen's catalyst........................................... 47
Oxazepam.............................................................32
AQC Phenylalanine............................................. 18 α-N,N-dimethylaminophenyl acetonitrile....48
Fluoxetine.............................................................32
AQC Serine............................................................ 19 2-amino-1,2-diphenylethanol..........................48
Mianserin..............................................................32
AQC Tyrosine....................................................... 19 phenylalaninol............................................... 48, 49
Tranylcypromine.................................................33
Fmoc Alanine....................................................... 19 4-chlorophenylalaninol..................................... 49
Trimipramine........................................................33
Fmoc Asparagine............................................... 19 trans-1-amino-2-indanol................................... 49
Venlafaxine...........................................................33
Fmoc Leucine......................................................20 Tryptophanol........................................................50
Fmoc Phenylalanine..........................................20 Beta blockers Tyrosinol................................................................50
Fmoc Tryptophan...............................................20 Acebutolol.............................................................34 α-(1-aminoethyl)-4-hydroxybenzyl-
Fmoc Valine..........................................................20 Alprenolol..............................................................34 alcohol...................................................................50
Methionine............................................................ 21 Atenolol..................................................................35 2-(2-chlorophenoxy)propionic acid...............50
p-chlorophenylalanine ..................................... 21 Esmolol..................................................................35 2-(3-chlorophenoxy)propionic acid............... 51
p-fluorophenylalanine....................................... 21 Isoproterenol .......................................................36 2-(4-chlorphenoxy)propionic acid................. 51
Phenylalanine...................................................... 21 Metaproterenol....................................................36 2-phenoxypropanoic acid................................ 51
Tryptophan...........................................................22 Metoprolol.............................................................37 2-amino-1,1,-diphenyl1-propanol.................... 51
α-amino-2-thiophenacetic acid......................22 Pindolol..................................................................37 2-amino-1,1,13-triphenyl-1-propanol.............. 52
3-amino-3-phenylpropanoic acid..................22 Propanolol............................................................38 2-(carbobenzyloxyamino)-3-phenyl-
Salbutamol...........................................................38 1-propanol............................................................. 52
Kynurenine............................................................22
Sotalol....................................................................39 cis-1-amino-2-indanol........................................ 52
Tryptophanamide...............................................23
3,4-dihydroxyphenylalanine (DOPA)............. 23 Terbutaline............................................................39
Tulobuterol............................................................40
Carvedilol..............................................................40
8
Neurotransmitters and Hormones Stimulants
Octopamine..........................................................53 3,4-methylenedioxyamphetamine
(MDA).....................................................................67
Oxazolidinones
3,4-methylenedioxymethamphetamine
1,5-dimethyl-4-phenyl-2-imidazolidinone....54 (MDMA).................................................................67
4-benzyl-2-oxazolidinone.................................54 3,4-dimethylmethcathinone............................67
4-methyl-5-phenyl-2-oxazolidinone..............54 4-methylethcathinone.......................................68
4-phenyl-2-oxazolidinone.................................54 Methoxamine.......................................................68
5,5-dimethyl-4-phenyl-2-oxazolidinone....... 55 Pentedrone...........................................................69
5,5-dimethyl-4-benzyl-2-oxazolidinone.......55 Pseudoephedrine........................................ 69, 70
5,5-dimethyl-4-methyl-2-oxazolidinone....... 55 α-pyrrolidinopentiophenone............................70
(3a-cis)-3,3a,8,8a-tetrahydro-2H- Norephedrine.......................................................70
indeno[1,2-d]oxazol-2-one................................55
Normetanephrine...............................................70
4-(1-H-indol-3-yl-methyl)-
Norphenylephrine...............................................71
2-oxazolidinone...................................................56
3,4-dihydroxyphenylalanine (DOPA)............. 71
Pharmaceuticals Clenbuterol...........................................................71
1-indanol................................................................57 Ethylone.................................................................72
Benzoin..................................................................57 Methylphenidate.................................................72
Chlophedianol......................................................57 3-fluoromethcathinone..................................... 73
Homatropine........................................................58 3,4-methylenedioxy-N-ethylamphetamine
Indapamide..........................................................58 (MDEA)..................................................................73
Lofexidine..............................................................58 4-ethylmethcathinone.......................................73
p-chloramphetamine........................................59 4-methylethcathinone.......................................73
Phenylsuccinic anhydride................................59 Aminorex............................................................... 74
Triadimenol...........................................................59 Amphetamine...................................................... 74
5-methyl-5-phenylhydantoin...........................59 Butylone ............................................................... 74
Aminoglutethimide............................................60 Cathinone.............................................................. 74
Bamethan.............................................................60 Flephedrone......................................................... 75
Diperodon...................................................... 60, 61 Mephedrone......................................................... 75
Flurbiprofen.......................................................... 61 Methamphetamine............................................ 75
Mandelic acid....................................................... 61 Methylephedrine................................................. 75
Naftopidil............................................................... 61 Methylone............................................................. 76
p-hydroxymandelic acid................................... 62 p-methoxyamphetamine................................. 76
Amlodipine............................................................62 Pentylone.............................................................. 76
Brompheniramine.............................................. 62 Synephrine........................................................... 76
Carbinoxamine.................................................... 62 α-pyrrolidinopentiophenone............................77
Cetirizine................................................................63
Chlorpheniramine...............................................63
Citalopram............................................................63
Disopyramide.......................................................63
Ketamine...............................................................64
Methoxyphenamine...........................................64
Methoxyverapamil.............................................64
Nicardipidine........................................................64
Orphenadrine.......................................................65
Piperoxan..............................................................65
Promethazine......................................................65
Tetrahydrozoline.................................................65
Thalidomide.........................................................66
Triahexylphenidyl................................................66
Trimebutine..........................................................66
Verapamil..............................................................66
9
Amines
1-benzyl-2,2-diphenylethylamine
Retention (min): 2.61/2.89 (Peak1/Peak 2)
Resolution: 3.51
Method Conditions
NH2 Column: NH2 Poroshell 120 Chiral-CD (15 cm x 4.6 mm, 2.7 μm)
InfinityLab
Mobile phase: 30/70: Acetonitrile/15 mM Ammonium Formate (pH 3.6)
Flow Rate: 1.0 mL/min
0 1 2 3 4 5 Temperature: Ambient (23 °C)
Injection Volume: 1.0 μL
Detection: UV 220 nm
0 1 2 3 4 5
1-benzyl-2,2-diphenylethylamine
Resolution: 9.47
Method Conditions
NH2
Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 1 2 3 4 5 Mobile phase: 100/0.1 wt %: Methanol/Ammonium Trifluoroacetate
Temperature: NH2 (23 °C)
Ambient
0 1 2 3 4 5
1,1'-binaphthyl-2,2'-diamine
Resolution: 2.11
Method Conditions
NH2
0 1 2 3 4 5 6 7
Column: InfinityLab Poroshell 120 Chiral-CD (10 cm x 4.6 mm, 2.7 μm)
Mobile phase: 90/10/0.3/0.2: Acetonitrile/Methanol/Trifluoroacetate/TEA
Temperature: Ambient (23 °C)
NH2 1.0 μL
Injection Volume:
0 1 2 3 4 5 6 7
1,1'-binaphthyl-2,2'-diamine
Resolution: 1.31
NH2
NH2 Method Conditions

NH2
Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2. 7μm)
0 1 2 3 4 5
Mobile phase: 90/10: Methanol/15 mM Ammonium Formate (pH 3.6)
Flow Rate: 1.0 mL/min NH2
0 1 2 3 4 5
10
Amines
1-(1-naphthyl)ethylamine
Resolution: 2.29
NH2
NH2
Method Conditions
Mobile phase: 100/0.1 wt %: Methanol/Ammonium Trifluoroacetate
0 1 2 3 4
0 1 2 3 4
1-(1-naphthyl)ethylamine
NH2 Retention (min): 2.64/2.95 (Peak1/Peak 2)
Resolution: 3.36 NH2
Method Conditions
Column: InfinityLab Poroshell 120 Chiral-CF (4.6 x 100 mm, 2.7 μm)
0 1 2 3 4 5
Mobile phase: 60/40/0.3/0.2: Acetonitrile/Methanol/Acetic Acid/TEA
0 1 2 3 4 5
1-(1,1-biphenyl-4-yl)ethanamine
Resolution: 2.11
Method Conditions
NH2
Column: InfinityLab Poroshell 120 Chiral-CF (10 cm x 4.6 mm, 2.7 μm)
0 1 2 3 4 5 6 7
Mobile phase: 90/10/0.3/0.2: Acetonitrile/Methanol/Trifluoroacetate/TEA
NH2
0 1 2 3 4 5 6 7
1-(1,1-biphenyl-4-yl)ethanamine
Resolution: 2.59
Method Conditions
NH2 Column: InfinityLab Poroshell 120 Chiral-V (4.6 x 100 mm, 2.7 μm)
0 1 2 3 4 Mobile phase: 100/0.1 wt %: Methanol/Ammonium Trifluoroacetate
Time (min) Flow Rate: 1.0 mL/min
NH2
0 1 2 3 4
Time (min)
11
Amines
1-(4-methoxyphenyl)ethylamine
NH2 Retention (min): 2.64/2.95 (Peak1/Peak 2)
Method Conditions
0 1 2 3 4 5 6
Column: InfinityLab Poroshell 120 Chiral-CF (4.6 x 100 mm, 2.7 μm)
0 1 2 3 4 5 6
1-(4-methoxyphenyl)ethylamine
NH2
Resolution: 2.38
NH2
Method Conditions
0 1 2 3 4 5 Mobile phase: 100/0.2/0.05: Methanol/Acetic Acid/Ammonium Hydroxide
0 1 2 3 4 5
1-aminotetralin
Resolution: 2.81
NH2
NH2
Method Conditions
0 1 2 3 4 5 6 Mobile phase: 60/40/0.3/0.2: Acetonitrile/Methanol/Acetic Acid/TEA
0 1 2 3 4 5 6
1-aminotetralin
Resolution: NH2
4.48
NH2
Method Conditions
Column: InfinityLab Poroshell 120 Chiral-V (10 cm x4.6 mm, 2.7 μm)
0 1 2 3 4 5 6
0 1 2 3 4
Time (min)
12
Amines
6-methoxy-1,2,3,4-tetrahydro-1-naphthalenylamine
NH2
O Method Conditions
0 2 4 6 Mobile phase: 60/40/0.3/0.2: Acetonitrile/Methanol/Acetic Acid/TEA
Flow Rate: O 1.0 mL/min
0 2 4 6
6-methoxy-1,2,3,4-tetrahydro-1-naphthalenylamine
NH2
Resolution: 5.26
NH2
O Method Conditions
0 0.5 1 1.5 O
0 0.5 1 1.5
Time (min)
1-(2-methoxyphenyl)ethanamine
NH2 Resolution: 2.18
NH2
Method Conditions
O
Mobile phase: 100/0.2/0.05: Methanol/Acetic Acid/Ammonium Hydroxide
ORate:
0 1 2 3 4
Flow 1.0 mL/min
0 1 2 3 4
1-(4-chlorophenyl)ethylamine
NH2
Resolution: NH 1.31
2
Method Conditions
CI
0 2 4 6 8 10 12
Temperature: Ambient (23 °C) CI
0 2 4 6 8 10 12
13
Amines
1,1-diphenyl-1-fluoro-2-aminopropane
Resolution: 3.20
F
Method Conditions
NH2
0 0.5 1 1.5 2 2.5 3 Mobile phase: 100/0.1 wt %: Methanol/Ammonium Trifluoroacetate
Injection Volume: NH2
1.0 μL
0 1 2 3 4
Time (min)
1,1-diphenyl-2-aminopropane
Resolution: 3.33
Method Conditions
NH2 Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 1 2 3 4 Mobile phase: 100/0.1 wt %: Methanol/Ammonium Trifluoroacetate
Injection Volume: NH2
1.0 μL
0 1 2 3 4
Time (min)
1,2,2-triphenylethylamine
Resolution: 3.88

Mobile phase:
NH
100/0.1 wt %: Methanol/Ammonium Trifluoroacetate
0 0.5 1 1.5 2 2.5 3 3.5
Flow Rate: 1.0 mL/min 2

0 0.5 1 1.5 2 2.5 3 3.5
2-amino-4-methyl-1,1-diphenylpentanepropane
Resolution: 4.49
Method Conditions
NH2
0 0.5 1 1.5
Injection Volume: NH
1.0 μL
2
0 0.5 1 1.5
Time (min)
14
Amines
2-chloro-indan-1-ylamine
NH2
Resolution: 2.81NH2
CI
Method Conditions
CI
0 1 2 3 4 5 Mobile phase: 90/10: Methanol/15 mM Ammonium Formate (pH 3.6)
0 1 2 3 4 5
Time (min)
4-methoxy-α-methylbenzylamine
Resolution:NH2
NH2
5.58
O Method Conditions
O
0 1 2 3 4
0 1 2 3 4
Time (min)
bis(1-phenylethyl)amine
H Resolution: 5.04
N
H
N
Method Conditions
0 0.5 1 1.5
0 0.5 1 1.5
bis(α-methylbenzyl)amine
Resolution: 7.66
N
N
H
H
N
N
Method Conditions H
H
0 0.5 1 1.5 2 Mobile phase: 100/0.2/0.05: Methanol/Acetic Acid/Ammonium Hydroxide
0 0.5 1 1.5 2
15
Amines
Mexiletine
Resolution: 1.54
O Method Conditions
NH 2 O
0 5
Time (min)
10 15
Flow Rate: 0.5 mL/min NH 2
0 5 10 15
Time (min)
N-benzyl-α-methylbenzylamine
H Resolution: 5.71
N H
N
Method Conditions
0 0.5 1 1.5 2 Mobile phase: 100/0.1 wt %: Methanol/Ammonium Trifluoroacetate
0 0.5 1 1.5 2
Time (min)
N,α-dimethylbenzylamine
Resolution: 3.47
N
H
Method Conditions
N
H
0 1 2 3 4 5
Time (min)
0 1 2 3 4 5
Time (min)
α-methyl-4-nitrobenzylamine
O Retention (min): 16.25/17.10 (Peak1/Peak 2)

N
Resolution: O 1.16
O
N
Method O
Conditions
0 10 20 30
Time (min)
Injection Volume: 1.0 μL NH2
0 10 20 30
Time (min)
16
Amines
α-methylbenzylamine
NH2
Resolution: 1.97
NH2
Method Conditions
0 2 4 6
Time (min)
0 2 4 6 Detection: UV 210 nm
Time (min)
17
Amino acid & Derivatives
2-amino-2-pheylbutyric acid
NH2 Resolution: NH2
5.16
COOH
Method Conditions COOH
0 0.5 1 1.5 2 2.5 3
Column: InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
Time (min) Mobile phase: 30/70: Ethanol/50 mM Ammonium Formate (pH 3.6)
Temperature: 45 °C
0 0.5 1 1.5 2 2.5 3
Time (min)
4-nitrophenylalanine
O
Resolution:
O 1.50
O—N +
O—N +
HO
O
Method Conditions
0 2 4 6 8 10 NH2 Mobile phase:
HO
30/70: Methanol/15 mM Ammonium Formate (pH 3.6)
Flow Rate: 0.5 mL/min O
0 2 4 6 8 10 NH2
AQC Methionine
AQC Retention (min): 1.55/2.25 (Peak1/Peak 2)
O
Resolution: AQC4.00
HN O
OH
HN
Method Conditions
Column:
OH
InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
0 1 2 3 4 S Mobile phase: 50/50: Acetonitrile/15 mM Ammonium Formate (pH 4.0)
0 1 2 3 4 S
AQC Phenylalanine
AQC O Retention (min): 1.96/2.26 (Peak1/Peak 2)
HN AQC
Resolution: O
2.32
OH
HN
Method Conditions
OH
0 1 2 3 4 Mobile phase: 50/50: Acetonitrile/15 mM Ammonium Formate (pH 4.0)
0 1 2 3 4
18
AQC Serine
AQC Resolution:AQC 2.14
HN
O
O
OH HN
Method Conditions
OH OH
0 0.5 1 1.5 2 2.5 3 Mobile phase: 50/50: Acetonitrile/15 mM Ammonium Formate (pH 4.0)
Flow Rate: 1.0OH
mL/min
0 0.5 1 1.5 2 2.5 3
AQC Tyrosine
Retention AQC
(min): 3.11/3.46 (Peak1/Peak 2)
AQC O Resolution: O1.77
HN
OH HN
OH
Method Conditions
OH
0 1 2 3 4 5 6 Mobile phase: 50/50: Acetonitrile/15 mM Ammonium Formate (pH 4.0)
Temperature:
OH
Ambient (23 °C)
0 1 2 3 4 5 6 Detection: UV 220 nm
Fmoc Alanine
O
Resolution: O3.68
OH
HN
Fmoc Method Conditions
OH
HN
0 1 2 3 4 5 6
Time (min)
Flow Rate:
Temperature: Fmoc
0.5 mL/min
Ambient (23 °C)
0 1 2 3 4 5 6
Time (min)
Fmoc Asparagine

H Resolution: 1.51 O
Fmoc
N
OH H
NH2 N
MethodFmoc
Conditions OH
0 1 2 3 4 5
Column: NH2 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
InfinityLab Poroshell
Time (min)
0 1 2 3 4 5
Time (min)
19
Fmoc Leucine
H
O
Resolution: 3.20 O
Fmoc
N
OH H
N
MethodFmoc
Conditions OH
0 1 2 3 4 5 6
0 1 2 3 4 5 6
Time (min)
Fmoc Phenylalanine
O Retention (min):
O
2.81/3.55 (Peak1/Peak 2)
H
N Resolution: H
3.71
Fmoc OH
N
Fmoc
Method Conditions
OH
0 1 2 3 4 5 6
Time (min)
0 1 2 3 4 5 6 Detection: UV 220 nm
Time (min)
Fmoc Tryptophan
Fmoc O
Resolution: Fmoc
2.20 O
HN
OH HN
Method Conditions OH
HN
0 2 4 6 Mobile phase: 60/40: Methanol/15 mM Ammonium Formate (pH 3.6)
Temperature: HN Ambient (23 °C)
0 2 4 6
Time (min)
Fmoc Valine
Fmoc O
HN
Fmoc
Resolution: 1.55
O
OH
HN
Method Conditions
0 1 2 3 4 5
Column: OH
Time (min)
0 1 2 3 4 5
Time (min)
20
Methionine
O
S
Resolution: 6.76 O
OH
NH2 S
0 1 2 3 4 5 Mobile phase: NH
60/40: Methanol/Water
2
0 1 2 3 4 5
Time (min)
p-chlorophenylalanine
O O
Resolution: 2.81
OH
Cl
NH 2
OH
Method Conditions
NH 2
Column:Cl InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
0 1 2 3 4 5 6 Mobile phase: 30/70: Ethanol/50 mM Ammonium Formate (pH 3.6)
Temperature: 45 °C
0 1 2 3 4 5 6
Time (min)
p-fluorophenylalanine
O Retention (min): O
3.37/3.70 (Peak1/Peak 2)
Resolution: 1.90
OH
F
NH 2 OH
Method Conditions
NH 2
Column:F InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
0 2 4 6 Mobile phase: 30/70: Methanol/50 mM Ammonium Trifluoroacetate (pH 3.6)
0 2 4 6
Time (min)
Phenylalanine

NH2 Resolution: O 2.75
HO
NH2
MethodHO
Conditions
0 1 2 3 4 5 6
Mobile phase: 30/70: Ethanol/50 mM Ammonium Formate (pH 3.6)
Time (min)
0 1 2 3 4 5 6
Time (min)
21
Tryptophan
O
Resolution: O 3.04
HO
NH2
NH2
Method HO
Conditions
0 1 2 3 4 5 6 Mobile phase: 30/70: Ethanol/50 mM Ammonium Formate (pH 3.6)
NH
Temperature: 45 °C
0 1 2 3 4 5 6
NH
Time (min)
α-amino-2-thiophenacetic acid
Resolution: S 7.35 O
S O
OH
NH2
0 0.5 1 1.5 2 2.5 3
Mobile phase: NH2Methanol/50 mM Ammonium Formate (pH 3.6)
30/70:
Temperature: 45 °C
0 0.5 1 1.5 2 2.5 3 Detection: UV 254 nm
3-amino-3-phenylpropanoic acid
NH2 O Resolution: NH2 O
2.25
OH
0 1 2 3 4 5 6
Temperature: 35 °C
0 1 2 3 4 5 6
Kynurenine
Resolution: NH
3.612
O
NH2
O
OH Column: InfinityLab Poroshell 120 Chiral-V (15 cm x 4.6 mm, 2.7 μm)
O NH2 Mobile phase: NH2
O30/70: Methanol/50 mM Ammonium Formate (pH 3.6)
Temperature: 45 °C
3 4
in) Detection: UV 230 nm
0 1 2 3 4
Time (min)
22
Tryptophanamide
H
N Resolution: H
2.75
N
NH2
Method Conditions
0 1 2 3 4 5
O
Mobile phase: NH 2 mM Ammonium Formate (pH 3.6)
90/10: Methanol/15
Injection Volume: 1.0 μL NH 2
0 1 2 3 4 5
Detection: O
UV 220 nm
Time (min)
3,4-dihydroxyphenylalanine (DOPA)
Resolution: 2.95 O
O
HO
HO
OH OH
Method Conditions
HO
NH2
Column:HO
NH 2
0 0.5 1 1.5 2 2.5 3 Temperature: 45 °C
0 0.5 1 1.5 2 2.5 3
23
Analgesics
Methadone
Resolution: 2.21
O
N
N
Method Conditions
O
0 2 4 6 Mobile phase: 20/80: Acetonitrile/50 mM Ammonium Formate (pH 3.6)
Temperature: 45 °C
0 2 4 6
Time (min)
Methadone
N Resolution: 2.09
O
N
O
Method Conditions
0 5 10 Mobile phase: 90/10: Ethanol/15 mM Ammonium Formate (pH 3.6)
Time (min)
0 5 10
Time (min)
Proglumide
Resolution:
O
2.18
OH
O OH
O O
Method Conditions
N N
N
H
Column: NInfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
O Mobile phase: H30/70: Methanol/50 mM Ammonium Trifluoroacetate (pH 3.6)
Flow Rate:
O
0.3 mL/min
Temperature: 45 °C
0 5 10
Time (min)
Proglumide
Resolution:
O
1.77
OH
O OH
O
O Method Conditions
N
N
N Column: NInfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
H Mobile phase: H60/40/0.3/0.2: Acetonitrile/Methanol/Acetic Acid/TEA
O
Flow Rate:
O
0.5 mL/min
0 1 2 3 4 5
Time (min)
24
Analgesics
Indoprofen
Resolution: 2.10
O O
O O
OH
OH
Method Conditions
N
N Column: InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
Temperature: 15 °C
15 Detection: UV 254 nm
0 5 10 15
Time (min)
O
Ketorolac
Resolution: 2.68 N
O
N Method Conditions
OH
OH
Column:
Mobile phase:
O
O
Temperature: 45 °C
0 0.5 1 1.5 2 2.5 3
Time (min)
0 0.5 1 1.5 2 2.5 3
Time (min)
Methorphan
Retention (min):
O
Resolution:O 2.19/2.51 (Peak1/Peak 2)
3.34
Method Conditions
N
0 1 2 3 4 Mobile phase: 80/20: Methanol/15 mM Ammonium Formate (pH 3.6)
Temperature: N
Ambient (23 °C)
0 1 2 3 4
Time (min)
Nefopam
Resolution: 2.10
O
Method Conditions
0 2 4 6 N
Mobile phase:
O
Time (min)
Temperature: 45 °C
0 2 4 6
Detection:
N
UV 220 nm
Time (min)
25
Analgesics
Tramadol
O
Resolution: 1.90 O
OH
Method Conditions
N
0 5 10
OH
Time (min) Flow Rate:
N
Temperature:
0.3 mL/min
45 °C
0 5 10
Time (min)
26
Anesthetics
Hexobarbital
O Resolution: 1.54
O
H 3C NH
Method Conditions
O N O
H C NH
CH 3 Column: 3 InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
Time (min) Flow Rate: O 0.5NmL/minO
Injection Volume:
CH
1.0 μL 3
0 5 10 15
Time (min)
Hexobarbital
O Resolution: 2.92
NH O
Method Conditions
O N O
Column: InfinityLab NHPoroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 2 4 6 8 10
Time (min)
Temperature:O N (23O°C)
Ambient
0 2 4 6 8 10
Time (min)
Mepivacaine
N
H Resolution: 1.42
N
N
H
O Method ConditionsN
0 2 4
Time (min)
6 8
Mobile phase: O
100/0.2/0.05: Methanol/Acetic Acid/Ammonium Hydroxide
0 2 4 6 8
Time (min)
Mepivacaine
H
N Resolution: 2.25 N
N H
O N
Method Conditions
0 2 4 6 8 10
Mobile phase: O
Time (min)
Temperature: 30 °C
0 2 4 6 8 10
Time (min)
27
Anesthetics
Bupivicaine
H Resolution: 3.76
N
N H
O N
Method Conditions N
Column: O
InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 0.5 1 1.5 2 2.5 3
0 0.5 1 1.5 2 2.5 3
Time (min)
Mephobarbital
Resolution: 2.63
O O
N
Method Conditions
O N
H
O
Column: N InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
Time (min)
O
Temperature: N 45 °CO
Injection Volume: H 1.0 μL
0 1 2 3 4 5
Time (min)
Prilocaine
Resolution: 2.31 O
H
O H
N N
N
H
Method Conditions
N
H
Mobile phase: 100/0.2/0.1: Methanol/Acetic Acid/Triethylamine
0 2 4 6 8 10
Time (min) Temperature: Ambient (23 °C)
0 2 4 6 8 10
Time (min)
28
Anticoagulants
Coumachlor
Resolution:
O 3.06 O Cl
O O Cl
Method Conditions
OH Column: OHInfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
O Mobile phase: 30/70: Acetonitrile/15 mM Ammonium Acetate (pH 3.6)
Flow Rate: O
1.0 mL/min
0 1 2 3 4
Temperature: 45 °C
Time (min) Injection Volume: 1.0 μL
0 1 2 3 4
Time (min)
Warfarin
Retention (min):
Resolution:
O3.38 O
3.75/4.45 (Peak1/Peak 2)
O O
OH Method Conditions
0 1 2 3 4 5 6 7
O Column:
Mobile phase:
OH
Temperature: 45 °C O
0 1 2 3 4 5 6 7
Time (min)
29
Anticonvulsants
Phensuximide
Retention (min): O4.50/4.91 (Peak1/Peak 2)
O
N
Resolution: 2.37 N
O
O
Method Conditions
0 2 4 6 8
Time (min)
Phensuximide
O
N Resolution: O 2.04
O N
Method Conditions O
0 5 10 15
0 5 10 15
Time (min)
α-methyl-α-phenylsuccinimide
O
Resolution: O 1.92
HN Method Conditions
O Column: HN InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
Temperature: 25 °C
O
0 5 10 15
Time (min)
30
Antidepressants
Bupropion
Resolution: 1.54 O H
Cl N
O
H
Cl N Method Conditions
20
0 5 10 15 20
Time (min)
Temazepam
N
O
Resolution: 4.63 O
OH N
CI N
Column:CI N
InfinityLab Poroshell 120 Chiral-CD (15 cm x 4.6 mm, 2.7 μm)
0 1 2 3 4 5 Mobile phase: 20/80: Acetonitrile/50 mM Ammonium Formate (pH 3.6)
Time (min)
0 1 2 3 4 5
Time (min)
Lorazepam
H
N
O Retention (min):
H O
0.69/1.22 (Peak1/Peak 2)
OH
Resolution: 8.65
N
N
CI
Method Conditions
OH
CI
Column:CI
N
0 0.5 1 1.5 2 Mobile phase: 100: Methanol
Time (min) Flow Rate: 2.0 mL/min CI
0 0.5 1 1.5 2
Time (min)
Lormetazepam
Resolution: 2.71
CI
CI N CI
N
O Column:CI InfinityLabNPoroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
0 0.5 1 1.5 2 2.5 3
Mobile phase: 100: Methanol
Time (min) Flow Rate: 1.0 mL/min OH
Temperature: 10 °C
Injection Volume: N
1.0 μL
Detection: UV 220 nm O
0 0.5 1 1.5 2 2.5 3
Time (min)
31
Antidepressants
Mianserin
N Resolution: 1.50
N
N
Method Conditions
Column: NInfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
0 5 10 Mobile phase: 100/0.1 wt %: Methanol/Ammonium Trifluoroacetate
0 5 10
Time (min)
Mianserin
Resolution: 9.77
N
Method Conditions
0 1 2 3
N
Column: N
Time (min)
Temperature: 45 °C
Injection Volume: N1.0 μL
0 1 2 3
Time (min)
Oxazepam
H O Retention (min): H (Peak1/Peak
0.72/1.33 O 2)
N Resolution: 9.49 N
OH
CI N OH
Method Conditions
CI N
0 0.5 1 1.5 2 Mobile phase: 100: Methanol
0 0.5 1 1.5 2
Time (min)
Fluoxetine
Resolution: 3.46 F
F
F
F F
F Method Conditions
N O Column: N O
H H
1 2 3 4
0Time (min) 1 2 3 4
Time (min)
32
Antidepressants
Tranylcypromine
Resolution: 2.53
NH2
NH2
Method Conditions
Temperature: 30 °C
0 2 4 6 8 10
Time (min)
Trimipramine
Resolution: 3.37
N
Method Conditions
N
0 1 2 3 4 N Mobile phase: 100/0.1 wt %: Methanol/Ammonium Trifluoroacetate
0 1 2 3 4 N
Time (min)
Venlafaxine
O
Resolution: O
2.12
N
0 2 4 6 8 10 Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
Time (min) Mobile phase: 100/0.2/0.1: Methanol/Acetic Acid/Triethylamine
Temperature: OH
Ambient (23 °C)
Injection Volume: 1.0 μL N
0 2 4 6 8 10
Time (min)
33
Beta blockers
Acebutolol
Retention (min): 7.08/7.63
O (Peak1/Peak 2)
H
Resolution: N 1.76
O
H
O O N
N Method Conditions H
OH
O Column: InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
O N
H
OH Mobile phase: 100/0.2/0.05: Methanol/Acetic Acid/Ammonium Hydroxide
0 5 10 Detection: UV 220 nm
5 10
Time (min)
Time (min)
Acebutolol
Retention (min): H O (Peak1/Peak 2)
7.01/7.56
Resolution: N 2.12
H
O O O H
N
Method Conditions OH N
O
O N
H
OH
0 2 4 6 8 10 12 Detection: UV 230 nm
Alprenolol
Resolution: 2.78
O N
Method Conditions H
OH
O N Column: InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
H
OH Mobile phase: 60/40/0.3/0.2: Acetonitrile/Methanol/Acetic Acid/TEA
0 1 2 3 4 5 6
0 1 2
Time (min) 3 4 5 6
Time (min)
Alprenolol
Resolution: 2.31
O N
H
OH
O
Method Conditions N
H
Column: OH Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
InfinityLab
0 1 2 3 4 5 6
0 1 2 3 4 5 6
34
Beta blockers
Atenolol
OH
H
N
Retention (min): O
6.56/7.31 (Peak1/Peak 2)
O
Resolution: 2.46
OH NH2
H
N O
O
Method Conditions
NH2
0 2 4 6 8 10 12 Injection Volume: 1.0 μL
Time (min)
Atenolol
Retention (min): OH 7.91/8.49 (Peak1/Peak 2)
H
Resolution: N 1.76
O
O
H
OH
Method Conditions NH2
N O
O
NH2 Mobile phase: 100/0.2/0.05: Methanol/Acetic Acid/Ammonium Hydroxide
0 5 10 15
15
Time (min)
Esmolol
Retention (min): 11.55 /12.66 (Peak1/Peak
O 2)
Resolution: 2.70
O
O N O
Method Conditions
H
OH
O
N O
H
OH
Mobile phase: 100/0.1 wt %: Methanol/Ammonium Formate
0 5 10 15 20
20 Time (min)
Esmolol
Retention (min): O
3.48/3.73 (Peak1/Peak 2)
O
Resolution: 2.02
O O
N O N O
H H
OH
2 4 6 Injection Volume: 1.0 μL
Time0 (min) 2 4 6 Detection: UV 220 nm
Time (min)
35
Beta blockers
Isoproterenol
OH
Resolution: 2.70 H
OH
HO N
H
HO N
Method Conditions
HO
Column: HO
Mobile phase: 100/0.2/0.1: Methanol/Trifluoroacetate/Ammonium Hydroxide
0 5 10 15 Temperature: 45 °C
Time (min)
0 5 10 15
Time (min)
Isoproterenol
OH Retention (min): 4.60/4.96 (Peak1/Peak 2)
H
Resolution: 1.0 OH
HO N
H
HO N
HO
Method Conditions
Column:HO InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 2 4 6 8 10
Time (min)
0 2 4 6 8 10
Time (min)
Metaproterenol
OH Retention (min): 1.62/1.89OH
(Peak1/Peak 2)
Resolution: 3.01
N OH
H
Column:
N OH
H InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
0 0.5 1 1.5
Time (min)
2 2.5 3
Mobile phase: OH
60/40/0.3/0.2: Acetonitrile/Methanol/Acetic Acid/TEA
Temperature: 45 °C
0 0.5 1 1.5 2 2.5 3 Detection: UV 230 nm
Time (min)
Metaproterenol
OH Resolution: 1.89 OH
N OH
H Method Conditions
OH
Column: N OH120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
Mobile phase: H 100/0.2/0.05: Methanol/Acetic Acid/Ammonium Hydroxide
0 2 4 6 8
Flow Rate: OH
0.5 mL/min
Time (min)
0 2 4 6 8
Time (min)
36
Beta blockers
Metoprolol
O Resolution: 3.01 OH
O N
H
Column: N OH120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
Mobile phase: H 60/40/0.3/0.2: Acetonitrile/Methanol/Acetic Acid/TEA
Flow Rate: OH
3.5 mL/min
Temperature: 45 °C
10
0 0.5 1 1.5 2 2.5 3
Time (min)
Metoprolol
O RetentionO(min): 4.80/5.13 (Peak1/Peak 2)
Resolution: 1.99
O N
H O N
OH
H
4 6 8 10 Temperature: Ambient (23 °C)
Time (min) Detection: UV 220 nm
0 2 4 6 8 10
Time (min)
Pindolol
Resolution: 2.56
N O NH
H
OH
N O NH
H
OH
Method Conditions
0 2 4 6 Flow Rate: 1.0 mL/min
0 2 4 6 8
Time (min)
Pindolol
Resolution: 2.33
N O NH
H
OH
N O NH
H
OH
Method Conditions
Time (min)
37
Beta blockers
Propanolol
Resolution: 3.11
N O
H
OH
N O
H
Method Conditions
OH
0 1 2 3 4 5 6
0 2 4 6 8
Time (min)
Propanolol
Resolution: 2.45
N O
H N O
OH H
Method Conditions
OH
0 1 2 3 4 5 6 Temperature: Ambient (23 °C)
0 1 2 3 4 5 6
Time (min)
Salbutamol
OH
Retention (min): OH 2)
5.69/6.34 (Peak1/Peak
Resolution: 2.66
N OH
H
OH
N
Method Conditions
OH
H
Column: OH
0 2 4 6 8 Flow Rate: 1.0 mL/min
Time (min) Temperature: 45 °C
0 2 4 6 8 10 Detection: UV 230 nm
Time (min)
Salbutamol
Resolution: 2.90 OH
OH
N
H
N OH Column: OH
H Mobile phase: 100/0.2/0.05: Methanol/Acetic Acid/Ammonium Hydroxide
OH
0 2 4 6 8 Detection: UV 220 nm
0 2
Time (min) 4 6 8
Time (min)
38
Beta blockers
Sotalol
OH Retention (min): 5.97/6.73 (Peak1/Peak 2)
H
N Resolution: 2.77 OH H
O O N
S
O O
Method Conditions
S
0 1 2 3 4 5 6
0 2 4 6 8 10 12
Time (min)
Sotalol
OH
OH
Resolution: 3.28 H
H N
N
O O O O
S S
Method Conditions
0 1 2 3 4 5 6 Temperature: Ambient (23 °C)
0 1 2 3 4 5 6
Time (min)
Terbutaline
OH
Retention (min): 2.92/3.74 OH
(Peak1/Peak 2)
Resolution: 6.48
N OH
H
OH
Method Conditions
Column:
N OH
0 1 2 3 4
H
Time (min) Mobile phase: OH
100/0.2/0.1: Methanol/Acetic Acid/Triethylamine
0 1 2 3 4 5 6 Detection: UV 220 nm
Time (min)
Terbutaline
OH
Resolution: 2.34 OH
N OH
H
Column: N OH120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 1 2 3 4
Time (min)
Flow Rate: OH
1.0 mL/min
0 1 2 3 4
Time (min)
39
Beta blockers
Tulobuterol
OH
Resolution: 3.51
H
OH
H
N
N
Method Conditions
Cl Column: InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
Mobile phase: Cl
0 1 2 3 4 5
Time (min)
Tulobuterol
Retention (min):
OH
1.65/1.82 (Peak1/Peak 2)
Resolution: 2.94
H
OH
H
N
N Method Conditions
Cl Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
Mobile phase: Cl
100/0.2/0.05: Methanol/Acetic Acid/Ammonium Hydroxide
0 1 2 3
Time (min)
Carvedilol
NH Retention (min): 7.76/8.37 (Peak1/Peak 2)
Resolution: 1.50 NH
O
N O
H
O
OH O
Method Conditions N O
H
Column: InfinityLab
OH Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
O
5 10 15
0 5 10 15
Time (min)
40
Depressants
Baclofen
CI Retention (min): 2.11/2.50 (Peak1/Peak 2)
Resolution: CI
2.87
O
Method Conditions
H2N
OH
0 1 2 3 4 Column: InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
Mobile phase:
O
100/ 0.1 wt %: Methanol/Ammonium Trifluoroacetate
Temperature: 45 °C
H 2N
Injection Volume: 1.0 μL OH
0 1 2 3 4
Baclofen
CI Retention (min): CI
2.05/2.29 (Peak1/Peak 2)
Resolution: 1.83
O
H2N Method Conditions
OH
0 1 2 3 4
Mobile phase: O
H 2N
Injection Volume: 1.0 μL OH
Octoclothepin
N Resolution: 2.23
N
N
Method Conditions
CI N
S
Time (min) Flow Rate: 0.7 mL/min CI
Temperature: 45 °C
Detection: SUV 230 nm
0 2 4 6 8
Time (min)
Tolperisone
Resolution: O
3.05
O
N
Method Conditions
N
0 1
Time (min) 2 3 4
Time (min)
41
Diuretics
Chlorthalidone
OH Cl Resolution: 2.45
OH CI
NH S O
O Method Conditions
O NH2
Column: S O 120 Chiral-CD (15 cm x 4.6 mm, 2.7 μm)
NH InfinityLab Poroshell
O
Mobile phase: NH2 mM Ammonium Formate (pH 3.6)
10/90: Acetonitrile/50
0.7 mL/minO
10 12
Flow Rate:
Temperature: 40 °C
0 2 4 6
Time (min)
Chlorthalidone
OH Cl Resolution: 2.54
OH Cl
NH S O
O
O NH2 Method Conditions
Column: S O 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
NH InfinityLab Poroshell
O
O NH2
0 2 4 6 8 10 12 Mobile phase: 30/70: Methanol/16 mM Ammonium Formate (pH 3.6)
0 2 4 6 8 10 12
Time (min)
Bendroflumethazide
Resolution: 2.79
H2N O O O
H2N O O O
S S
O NH S S
F N O NH
F Method Conditions
F H
F N Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
Column: InfinityLab
F
Mobile phase:F 30/70:HMethanol/50 mM Ammonium Trifluoroacetate (pH 3.6)
8 10
Temperature: 45 °C
0 2 4 6 8 10 Detection: UV 220 nm
Time (min)
42
Fungicides
Benalaxyl
O O Retention (min): 1.64/1.87 (Peak1/Peak 2)
O
Resolution: 3.13 O O
N
O
Method Conditions N
2 2.5 3 Column: InfinityLab Poroshell 120 Chiral-CD (10 cm x 4.6 mm, 2.7 μm)
n) Mobile phase: 30/70: Acetonitrile/15 mM Ammonium Formate (pH 3.6)
0 0.5 1 1.5 2 2.5 3
Time (min)
Econazole
CI
Retention (min): 3.17/3.43CI
(Peak1/Peak 2)
Resolution: 2.15
CI O N
Method Conditions
N
4 5 6 Column: InfinityLab Poroshell 120 Chiral-CD (10 cm x 4.6 mm, 2.7 μm)
min) CI Mobile phase: 30/70: Acetonitrile/15 mM Ammonium Trifluoroacetate (pH 3.6)
Flow Rate: CI O
0.7 mL/min N
Injection Volume: 1.0 μL N
0 1 2 3 4 5 6 Detection: UV 220 nm
Time (min) CI
Flutriafol
F Retention (min):
Resolution: F
2.89 /3.14 (Peak1/Peak 2)
2.54
F N
N N
Method Conditions
3 4 5
OH
me (min) Column: InfinityLab Poroshell 120 Chiral-CD (15 cm x 4.6 mm, 2.7 μm)
F
Mobile phase: N
30/70: Acetonitrile/50 mM Ammonium Formate (pH 3.6)
Flow Rate:
Temperature:
1.0 mL/min
15 °C N N
0 1 2 3 4 5
Detection: OH
UV 230 nm
Time (min)
Miconazole
Resolution: 2.18
N
N N
N
O CI
Method Conditions
CI CI O CI
CI
CI
Flow Rate:
CI
0.7 mL/min
4 5 6
Temperature: 40 °C
Injection Volume: 1.0 μL CI
0 1 2 3 4 5 6
Time (min)
43
Fungicides
Praziquantel
O Resolution: O 1.85
N
N N
Method Conditions
O Column:
N
0 5 10 15 Flow Rate: O
0.7 mL/min
0 5 10 15
Time (min)
Prothioconazole
Retention (min):
S CI 2)
2.86/3.16 (Peak1/Peak
S CI Resolution: 2.59OH
OH
N N
MethodNConditions
N
NH
CI NH
Mobile phase: CI
4 5
0 1 2 3 4 5
Time (min)
Dichloroprop
Resolution: CI 4.91 O
O
CI O OH
Method Conditions
O
OH Column:CI InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
CI Mobile phase: 100/0.3 wt %: Methanol/Ammonium Trifluoroacetate
0 0.5 1 1.5 2 2.5 3 Detection: UV 220 nm
0 0.5 (min) 1
Time 1.5 2 2.5 3
Time (min)
Fenoprop
CI O Resolution: 3.00
O
OH
CI O
CI CI OH
Method Conditions
Column:CI CI Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
InfinityLab
0 2 4 6 8
Mobile phase: 30/70: Methanol/50 mM Ammonium Trifluoroacetate (pH 3.6)
Temperature: 45 °C
0 2 4 6 8
Time (min)
44
Fungicides
Haloxyfop
F O
F Retention (min): 1.12/1.37 (Peak1/Peak 2)
CI O
F OH Resolution:
F 4.48 O
F
N O CI O
F OH
Method Conditions
N O
0.5 1 1.5 2 2.5
0 0.5 1 1.5 2 2.5 Detection: UV 220 nm
Time (min)
Mandipropamid
Cl
Retention (min): Cl 7.65/8.22 (Peak1/Peak 2)
Resolution: 1.87
H
N O
O
O H
O
Method Conditions
O
N O
Column: O InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
O
5 10 15
Temperature: 45 °C
0 5 10 15
Time (min)
Mecoprop methyl ester

Retention (min):
Resolution:
O
12.73/13.39 (Peak1/Peak 2)
1.74
O
O O
Method Conditions
O
Column: O
Cl
0 5 10
Time (min)
15 20
Cl
Temperature: 45 °C
0 5 10 15 20
Time (min)
Mecoprop
O
OH
Resolution: 3.59
OH
O
O
CI
O
Method Conditions
0 0.5 1 1.5
Time (min)
2 2.5
CI
0 0.5 1 1.5 2 2.5
Time (min)
45
Fungicides
Napropamid
Resolution: 2.26 O
O O
O N
N Method Conditions
6 Temperature: 45 °C
0 1 2 3 4 5 6 Detection: UV 220 nm
Time (min)
Napropamid
Resolution: 1.76 O
O
O Method Conditions
N
O
N Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 2 4 6
Time (min)
Bromacil
O Resolution: 2.10 O
Br
N Br
O N
Method Conditions N
H Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 2 4 6 8 Mobile phase:
Flow Rate: O N
0.5 mL/min
H
Time (min)
0 5 10 15
Time (min)
Bromacil
Br Resolution: 2.33 O
N
O
Br
Method Conditions N
N
H
0 2 4 6 8 Column: InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
Mobile phase: 30/70: Methanol/50 mM Ammonium Trifluoroacetate (pH 3.6)
Time (min)
Flow Rate: O N
0.5 mL/min
Temperature: 45 °C H
0 2 4 6 8
Time (min)
46
Miscellaneous
2'-amino-1,1'-binaphthalen-2-ol
Resolution: 1.45
OH
NH2
Method Conditions
OH
0 1 2 3 4 Column: InfinityLab Poroshell 120 Chiral-CD (15 cm x 4.6 mm, 2.7 μm)
NH2
0 1 2 3 4
Hydratropic acid
1.23/1.44 (Peak1/Peak 2)
O
Resolution: 3.36
OH
O
OH
Method Conditions
0 0.5 1 1.5 2 2.5 Mobile phase: 100/0.3 wt %: Methanol/Ammonium Trifluoroacetic Acid
0 0.5 1 1.5 2 2.5 Detection: UV 220 nm
Time (min)
Tropic acid
Resolution: 1.98 O
O
HO OH MethodHO
Conditions OH
2 3 Temperature: Ambient (23 °C)
(min) Injection Volume: 1.0 μL
0 1 2 3
Time (min)
Jacobsen's catalyst
Resolution: 3.23
N N
Mn N N
Mn
O CI O Method ConditionsO CI O
6 Injection Volume: 1.0 μL
0 1 2 3 4 5 6
Time (min)
47
Miscellaneous
α-N,N-dimethylaminophenyl acetonitrile
N
Resolution: 3.59
N
N N
Method Conditions
0 2 4 6 8
2-amino-1,2-diphenylethanol
O
N O
N
Method Conditions
5 6
0 1 2 3 4 5 6 Detection: UV 220 nm
Time (min)
2-amino-1,2-diphenylethanol
NH2 Resolution: NH2
1.58
0 2 4 6 8 Mobile phase: OH Methanol/Acetic Acid/Ammonium Hydroxide
100/0.2/0.05:
0 2 4 6 8
phenylalaninol
Resolution:HO 2.35
HO
NH2 NH2
Method Conditions
Column: InfinityLab Poroshell 120 Chiral-CF(10 cm x 4.6 mm, 2.7 μm)
48
Miscellaneous
phenylalaninol
Resolution: 1.96 NH
2
OH
OH Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
3 4
0 1 2 3 4
4-chlorophenylalaninol
Resolution: 2.34 OH
NH2
OH CI
Method Conditions
NH2
CI Column: InfinityLab Poroshell 120 Chiral-CF (10 cm x 4.6 mm, 2.7 μm)
0 2 4 6
0 2 4 6
trans-1-amino-2-indanol
OH Resolution: 4.38
NH2
OH
Method Conditions
NH
0 2 4 6
Time (min) Mobile phase: 60/40/0.3/0.2:
2 Acetonitrile/Methanol/Acetic Acid/TEA
0 2 4 6
Time (min)
trans-1-amino-2-indanol
Resolution: 3.67
OH
OH
0 1
Time (min)
2 3 4
Mobile phase: NH
100/0.1 wt2 %: Methanol/Ammonium Trifluoroacetate
0 1 2 3 4
Time (min)
49
Miscellaneous
Tryptophanol
HO Retention (min): 3.39/3.74 (Peak1/Peak 2)
Resolution: HO
2.88
NH2
N
Method Conditions
0 2
Time (min)
4 6 H
Column: NH
2 120 Chiral-CF (10 cm x 4.6 mm, 2.7 μm)
Injection Volume: N
1.0 μL
0 2 4 6
Detection: H
UV 280 nm
Time (min)
Tyrosinol
HO
HO Resolution: 2.88 NH2
OH
N
0 2 4 6 H Mobile phase: 60/40/0.3/0.2: Acetonitrile/Methanol/Acetic Acid/TEA
Time (min)
α-(1-aminoethyl)-4-hydroxybenzyl-alcohol
Resolution: 1.91 NH 2
NH2
OH Method Conditions OH
HO Column: InfinityLab Poroshell 120 Chiral-CF (10 cm x 4.6 mm, 2.7 μm)
0 5 10
HO
0 5 10
Time (min)
2-(2-chlorophenoxy)propionic acid
CI O
Resolution:
CI 2.95 O
O
OH
O
0 2 4 6 Column: InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
Time (min) Mobile phase: 100/0.3 wt %: Methanol/Ammonium Trifluoroacetate
0 2 4 6
Time (min)
50
Miscellaneous
2-(3-chlorophenoxy)propionic acid (Metachlorphenprop)
O
Retention (min): O
1.24/1.38 (Peak1/Peak 2)
Resolution: 2.51
CI O CI O
OH
OH
Method Conditions
0 0.5 1 1.5 2 2.5 3 Flow Rate: 1.0 mL/min
0 0.5 1 1.5 2 2.5 3
Time (min)
2-(4-chlorphenoxy)propionic acid
O Resolution: 4.92
O
O
OH O
CI
OH
Method Conditions
Column:CI InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
0 0.5 1 1.5 2 2.5 3 Mobile phase: 100/0.3 wt %: Methanol/Ammonium Trifluoroacetate
0 0.5 1 1.5 2 2.5 3
Time (min)
2-phenoxypropanoic acid
Resolution: 3.36 O
O O
O
OH
Mobile phase: 100/0.3 wt %: Methanol/Ammonium Trifluoroacetic Acid
0 0.5 1 1.5 2 2.5 Temperature: Ambient (23 °C)
0 0.5 1 1.5 2 2.5
Time (min)
2-amino-1,1,-diphenyl-1-propanol
Resolution: 2.66
HO
Method Conditions
NH2
0 1 2 3 4 Column: HO InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
Injection Volume: 1.0 μLNH2
0 1 2 3 4
51
Miscellaneous
2-amino-1,1,13-triphenyl-1-propanol
Resolution: 4.66
OH
NH2
Method Conditions
0 0.5 1 1.5 2 2.5
Mobile phase:
OH
Flow Rate: 1.0NH 2
mL/min
0 0.5 1 1.5 2 2.5 Detection: UV 220 nm
2-(carbobenzyloxyamino)-3-phenyl-1-propanol
Retention (min): 13.85/14.94OH
(Peak1/Peak 2)
OH
O Resolution: O
1.50
O N
H O N
Method Conditions H
0 5 10 15 20 25 Flow Rate: 0.5 mL/min
0 5 10 15 20 25
Time (min)
cis-1-amino-2-indanol
Resolution: NH
2.80 2
NH2
OH
0 1 2 3
Time (min)
52
Neurotransmitters and Hormones
Octopamine
HO HO
Resolution: 2.08
NH2 NH2
Method Conditions
OH Mobile phase: 60/40/0.3/0.2: Acetonitrile/Methanol/Acetic Acid/TEA
Detection: OH
UV 230 nm
0 2 4 6 8
Time (min)
53
Oxazolidinones
1,5-dimethyl-4-phenyl-2-imidazolidinone
N
Retention (min): N(Peak1/Peak 2)
1.81/1.99
Resolution: 2.66
N
O
O
H
Method Conditions N
H
0 1 2 3 4
0 1 2 3 4 Temperature: Ambient (23 °C)
4-benzyl-2-oxazolidinone
H
N Retention (min):
H
5.60/6.08 (Peak1/Peak 2)
O
O Resolution: 1.92
N
O
Method Conditions
0 2 4 6 8 10 Column:
O
Time (min)
0 2 4 6 8 10
Time (min)
4-methyl-5-phenyl-2-oxazolidinone
NH Resolution: 6.51 NH
O
O O
Method Conditions
O
0 0.2 0.4 0.6 0.8 1 1.2 Mobile phase: 100: Methanol
0 0.2 0.4 0.6 0.8 1 1.2
4-phenyl-2-oxazolidinone
Resolution: 2.79 O
O O
O Method Conditions N
N
H Column: H Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
InfinityLab
0 0.5 1 1.5 2 2.5 3
0 0.5 1 1.5 2 2.5 3
54
Oxazolidinones
5,5-dimethyl-4-phenyl-2-oxazolidinone
O
Resolution: 1.96 O
O
N
H O
Method Conditions N
Column: H Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
InfinityLab
0 1 2 3 4 5
Temperature: 35 °C
0 1 2 3 4 5 Detection: UV 220 nm
5,5-dimethyl-4-benzyl-2-oxazolidinone
Resolution: O
3.32 O
O O
NH
Method Conditions NH
0 0.5 1 1.5
Mobile phase: 100: Methanol
0 0.5 1 1.5
5,5-dimethyl-4-methyl-2-oxazolidinone
Resolution: 4.84
O O
NH Method Conditions O O
0 0.5 1 1.5 Mobile phase: NH
100: Methanol
0 0.5 1 1.5
Time (min)
(3a-cis)-3,3a,8,8a-tetrahydro-2H-indeno[1,2]-doxazol-2-one
Resolution: 1.91 H
H
O O
Method Conditions
O
H
N
H
Column: O 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
N
Mobile phase: H Hwt %: Methanol/Ammonium Trifluoroacetate
100/0.1
0 2 4 6
Time (min)
55
Oxazolidinones
4-(1-H-indol-3-yl-methyl)-2-oxazolidinone
H Resolution:
H
1.98
N N
H
N O
Method Conditions H
O
Column: InfinityLabN
PoroshellO120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 1 2 3 4 5 Mobile phase: 100: Methanol
Temperature: O
Ambient (23 °C)
0 1 2 3 4 5
56
Pharmaceuticals
1-indanol
OH
Resolution: 2.26
OH
Method Conditions
0 5 10 15
0 5 10 15
Benzoin
Resolution: O
2.11
O
Method Conditions
OH
Column:
OH
Temperature: 40 °C
6 8
Chlophedianol
Resolution: 2.53
HO N
Method Conditions
CI
HO N
CI
3 4 5 6
me (min) Flow Rate: 0.6 mL/min
0 1 2 3 4 5 6
Time (min)
Chlophedianol
Resolution: 2.53
HO N
Method Conditions
CI
Column:
HO N
0 1 2 3 4 5 6
Time (min)
CI
Flow Rate:
Temperature:
0.6 mL/min
Ambient (23 °C)
0 1 2 3 4 5 6
Time (min)
57
Pharmaceuticals
Homatropine
Resolution: 2.25
O
O
N N
Method Conditions
O O
OH Column: InfinityLab Poroshell 120 Chiral-CD (15 cm x 4.6 mm, 2.7 μm)
OH
4 5 Injection Volume: 1.0 μL
) Detection: UV 230 nm
0 1 2 3 4 5
Time (min)
Indapamide
Resolution: 1.12
O O
NH2
N S
N O O
H O
Method Conditions
NH2
Cl N S
Column:
N O 120 Chiral-CD (15 cm x 4.6 mm, 2.7 μm)
H InfinityLab Poroshell
0 5 10 15 20 25 Flow Rate: 0.3 mL/min Cl
0 5 10 15 20 25
Time (min)
Lofexidine
Retention (min):
Resolution:
CI
7.82/8.29 (Peak1/Peak 2)
1.97
CI
H
H
N Method Conditions N
CI
O
N
O
CI
Mobile phase: N
0 2 4 6 8 10
Time (min)
Lofexidine
Cl
H Resolution: Cl
1.15
N
O
H
Cl N
Method Conditions N
0 5 10 15 Column:
O
InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm) x2
Time (min)
Flow Rate:
Cl
Mobile phase:
N
100/0.2/0.05: Methanol/ Acetic Acid/Ammonium Hydroxide
0.5 mL/min
0 5 10 15
Time (min)
58
Pharmaceuticals
p-chloramphetamine
Cl Resolution: 1.54 Cl
NH 2 NH 2
Method Conditions
0 5 10 15 20 Mobile phase: 15/85: Acetonitrile/15 mM Ammonium Formate (pH 3.6)
0 5 10 15 20
Time (min)
Phenylsuccinic anhydride
O
Resolution: O2.83
O O
O
O
Method Conditions
0 0.5 1 1.5 2 2.5 3
Time (min)
0 0.5 1 1.5 2 2.5 3
Time (min)
Triadimenol
OH
Retention (min): 7.42/9.38 (Peak 1/Peak 2), 9.97/10.42 (Peak 3/Peak 4)
O
Resolution:
OH 7.49 (Peak 1/Peak 2), 1.63 (Peak 3/Peak 4)
N
N CI O
N Method Conditions
NInfinityLab Poroshell 120 Chiral-CD (15 cm x 4.6 mm, 2.7 μm)
0 5 10 15
Column:
N CI
Time (min)
Flow Rate: N 0.5 mL/min
Temperature: 35 °C
0 5 10 15
Time (min)
5-methyl-5-phenylhydantoin
1.26/1.59 (Peak1/Peak 2)
Resolution: 5.83
NH
N
NH
H O Method Conditions
Column: N
InfinityLab
O
Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
0 0.5 1 1.5 2 2.5 3 Mobile phase: 100:HMethanol
0 0.5 1 1.5 2 2.5 3 Detection: UV 220 nm
59
Pharmaceuticals
Aminoglutethimide
NH2 Resolution: 2.50 NH 2
Method Conditions
0 2 4 6 8 O N O Mobile phase: 100/0.5 wt %: Methanol/Ammonium Trifluoroacetate
H
Temperature: 10 °C
0 2 4 6 8 O
Detection: N O
UV 230 nm
H
Time (min)
Bamethan
OH
OH Resolution: 1.52 H
H N
N
HO Method
HOConditions
0 5 10 15
Time (min)
Time (min)
Bamethan
OH (Peak1/Peak 2)
Resolution: 2.24 H
N
OH
H
N MethodHO
Conditions
HO Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
10 0 2 4 6 8 10 Detection: UV 220 nm
Time (min)
Diperodon
N Retention (min): 8.80/9.87 (Peak1/Peak 2)
Resolution: 2.44
O O N
H
N O NH
O Method Conditions O O
H
Column: N InfinityLab
O Poroshell
NH 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
0 5 10 15 Mobile phase: 100/0.2/0.05: Methanol/Acetic Acid/Ammonium Hydroxide
Time (min) Flow Rate: O0.7 mL/min
0 5 10 15
Time (min)
60
Pharmaceuticals
Diperodon
N
O
H Resolution: 1.40
N O N
O N O
O
H H
N O
O N
Method Conditions H
O
5 10 15 Flow Rate: 0.5 mL/min
Time (min)
Time (min)
Flurbiprofen
Resolution: 1.50
OH OH
O
F
Method Conditions O
Mobile phase: F Methanol/15 mM Ammonium Formate (pH 3.6)
30/70:
Time (min)
Mandelic acid
Resolution: 3.56
O
O
OH Column: OH
Mobile phase: 30/70: Methanol/80 mM Ammonium Acetate (pH 5.0)
0 0.5 1
Time (min)
1.5 2
Flow Rate: OH
0.5 mL/min
Temperature: 45 °C
0 0.5 1 1.5 2
Time (min)
Naftopidil
Resolution: 1.91 O
H
O OH N
Method Conditions
O O N
N Column: InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
H H
0 5 10 15
Time (min)
61
Pharmaceuticals
p-hydroxymandelic acid
HO
Resolution: 2.87
O
HO
O
OH
OH Column: InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
Mobile phase: OH
1 1.5 2
e (min) Temperature: 45 °C
0 0.5 1 1.5 2
Time (min)
Amlodipine
Cl Cl
Resolution: 2.06
O O
O O
O O
O Method Conditions
O O
N NH 2
H O Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
Column: InfinityLab
N NH 2
5 10 15 20
0 5 10 15 20 Detection: UV 220 nm
Time (min)
Brompheniramine
Resolution: 2.05
N N
Method Conditions
N
Br N
0 2 4 6 8 10 Flow Rate: 0.7 mL/min
Time (min) Temperature: Ambient (23 °C) Br
0 2 4 6 8 10
Time (min)
Carbinoxamine
N Resolution: 1.92
N
O
N
Cl Method Conditions
N
Column: O
0 5 10 15
Time (min) Flow Rate: 0.5 mL/min Cl
0 5 10 15
Time (min)
62
Pharmaceuticals
Cetirizine
Resolution: 1.12
N N
Cl
N
O
OH Method Conditions N OH
Cl O
O Column: InfinityLab Poroshell
O 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm) x2
30
0 5 10 15 20 25 30 Detection: UV 230 nm
Time (min)
Chlorpheniramine
Resolution: 1.95
N N
Method Conditions
N
CI N
0 2 4 6 8 10 Flow Rate: 0.7 mL/min
Time (min) Temperature: Ambient (23 °C) CI
0 2 4 6 8 10
Time (min)
Citalopram
F
F (Peak1/Peak 2)
Resolution: 2.15
N
O
Method Conditions
N
Column:
N
0 5 10 15
Time (min)
Flow Rate: O
0.7 mL/min
NVolume:
Injection 1.0 μL
0 5 10 15
Time (min)
Disopyramide
Resolution: 2.12
O
O
NH2
N
Method Conditions
N NH2
0 2 4 6 8 10 Mobile phase: 60/40/0.3/0.2: Acetonitrile/Methanol/Acetic Acid/TEA
Time (min) Flow Rate: N
0.5 mL/min
0 2 4 6 8 10
Time (min)
63
Pharmaceuticals
Ketamine
Resolution: 1.22
Cl
Cl
NH
Method Conditions
O
NH
0 5 10 15 20
N
Injection Volume:
Detection: O
1.0 μL
UV 220 nm CI
0 5 10 15 20
CI CI
Time (min) N
CI
CI
CI CI
Methoxyphenamine CI

O
O
Resolution: 2.54
N
H
Method Conditions
0 1
Time (min)
2 3
H
Time (min)
Methoxyverapamil
O O Retention (min):16.03/17.26
O (Peak1/Peak
O 2)
O
Resolution: 1.17
N O
O N N
O
Method Conditions N
O
Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm) x2
O
10 20 30
Time (min)
Time (min)
Nicardipidine
H
H Resolution: 2.78 N
N
O O O O
N N
O O Method Conditions O O
O Column: InfinityLab
O Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
N N wt %: Methanol/Ammonium Trifluoroacetate
Mobile phase: 100/0.1
Temperature: 45 °C
0.5 1 1.5 Detection: UV 230 nm
0 0.5 1 1.5
Time (min)
Time (min)
64
Pharmaceuticals
Orphenadrine
Resolution: 2.92
N
O N
Method Conditions O
0 1 2 3 4
Time (min)
0 1 2 3 4
Time (min)
Piperoxan
O
N Retention (min): O4.72/5.12 (Peak1/Peak 2)
O
Resolution: 2.18 N
O
Method Conditions
0 2 4 6 8 Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
Time (min) Mobile phase: 100/0.2/0.1: Methanol/Acetic Acid/Triethylamine
Time (min)
Promethazine
N
N
Resolution: 7.52
N
Method Conditions
0 1 2 3 4 5
Time (min) S Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
N
Temperature: N NH
Ambient (23 °C)
0 1 2 3 4 5 Detection: UV 254 nm
Time (min) S
Tetrahydrozoline
Resolution: 2.49
N NH
Method Conditions
0 1 2 3
0 1
Time (min)
2
Time (min)
3
65
Pharmaceuticals
Thalidomide
Resolution:
O
8.50
N O
NH
O O
Method Conditions
N O
NH
0 0.5
Time (min)
1 1.5
Mobile phase: O O
100: Methanole
Temperature: 45 °C
0 0.5 1 1.5
Time (min)
Triahexylphenidyl
Resolution: 2.66
N
OH
Method Conditions
0 1 2 3 4
Time (min)
Mobile phase: N 100/0.1 wt %: Methanol/Ammonium Trifluoroacetate
Flow Rate: OH
1.0 mL/min
Time (min)
Trimebutine
O Resolution: O 2.98
O
N O
O
O N
O Method Conditions O
O
0 0.5 1 1.5 2 2.5 3 Mobile phase: O wt %: Methanol/Ammonium Trifluoroacetate
100/0.1
0 0.5 1 1.5 2 2.5 3
Time (min)
Verapamil
O
O Resolution: 1.13
O
O
N N
O N Method O
Conditions N
O Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm) x2
O
10 20 30 40 Injection Volume: 1.0 μL
Time (min) Detection: UV 230 nm
0 10 20 30 40
Time (min)
66
Stimulants
3,4-methylenedioxyamphetamine (MDA)
NH2
Resolution:
NH2 1.71 O
O
Method Conditions O
0 5 10 15 20
Time (min) Mobile phase: 10/90: Acetonitrile/15 mM Ammonium Formate (pH 3.6)
0 5 10 15 20
Time (min)
3,4-methylenedioxyamphetamine (MDA)
O Resolution: 1.16 H
NH2
O N
O
Method Conditions
Column:
O InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm) x2
0 5 10 15 20 Mobile phase: 95/5: Ethanol/15 mM Ammonium Formate (pH 3.6)
0 10 20 30 40 50 60
Time (min)
3,4-methylenedioxymethamphetamine (MDMA)
Resolution: 1.54
H
O
H
N O N
O
Method Conditions
O
0 5 10 Mobile phase: 15/85: Acetonitrile/15 mM Ammonium Formate (pH 3.6)
Time (min)
3,4-dimethylmethcathinone
H
N H
N
Method Conditions
Temperature: 45 °C
0 5 10 15
Time (min)
67
Stimulants
4-methylethcathinone
Resolution: O
1.13
H
O
H
N
N
Method Conditions
Column: InfinityLab Poroshell 120 Chiral-CD (10 cm x 4.6 mm, 2.7 μm) x2
0 5 10 15 20
0 5 10 15 20
H O
N Resolution: 1.52 H
N
Method Conditions
0 10 20 30 40 50
0 10 20 30 40 50
Time (min)
Methoxamine
O
Resolution: 3.62
HO
O O
H2N Method Conditions
0 1 2 3 4
Column: HO InfinityLab Poroshell 120 Chiral-CD (10 cm x 4.6 mm, 2.7 μm)
Time (min)
Mobile phase: O
HN 2
0 1 2 3 4
Time (min)
Methoxamine
Resolution: 1.24
O
O
HO
Method Conditions
O
Column: HO InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 5 10 15
H2N Mobile phase: O
Time (min)
H N 2
0 5 10 15
Time (min)
68
Stimulants
Pentedrone
O
H Retention (min): O
3.54/3.87 (Peak1/Peak 2)
N Resolution: 2.68 H
N
Method Conditions
0 2 4 6 Column: InfinityLab Poroshell 120 Chiral-CD (15 cm x 4.6 mm, 2.7 μm)
Time (min)
Pentedrone
O
H O
N Resolution: 1.80
H
N
Method Conditions
0 5 15 Column: InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
Time (min)
0 2 4 6
Time (min)
Pseudoephedrine
OH
Resolution:
H
8.98
OH
H
N
Method Conditions
N
0 2 4 6 Column: InfinityLab Poroshell 120 Chiral-CD (15 cm x 4.6 mm, 2.7 μm)
0 2 4 6
Time (min)
Pseudoephedrine
OH Resolution: OH
2.04
H
N H
Method Conditions
N
0 5 10 15
Time (min) Mobile phase: 60/40/0.3/0.2: Acetonitrile/Methanol/Acetic Acid/TEA
0 5 10 15
Time (min)
69
Stimulants
Pseudoephedrine
Resolution: OH
1.40
OH H
N
H
N
Method Conditions
0 5 10 15
Time (min)
α-pyrrolidinopentiophenone
Retention (min):
Resolution:
O
14.24/15.21 (Peak1/Peak 2)
1.34
O
N N
Method Conditions
0 5 10 15 20 25 30 Flow Rate: 0.3 mL/min
0 5 10 15 20 25 30 Detection: UV 254 nm
Time (min)
Norephedrine
OH
OH Resolution: 1.96
NH2
Method Conditions
0 2 4 6 8
Time (min)
Mobile phase:
Flow Rate:
NH
0.8 mL/min 2
0 2 4 6 8
Time (min)
Normetanephrine
O
OH
NH2
Retention (min): OH (Peak1/Peak 2)
4.46/4.96
Resolution: 2.88
O NH2
HO
Method Conditions
HO
0 2 4 6 8 Column: InfinityLab Poroshell 120 Chiral-CF (10 cm x 4.6 mm, 2.7 μm)
Time (min)
70
Stimulants
Norphenylephrine
OH
Retention (min):
Resolution: OH
4.30/4.77 (Peak1/Peak 2)
2.84
NH2
NH2
Method Conditions
OH
0 2 4 6 8
OH
Temperature:
Injection Volume:
Ambient (23 °C)
1.0 μL
Time (min)
3,4-dihydroxyphenylalanine (DOPA)
Resolution: 2.95 O
O
HO
HO
OH OH
Method Conditions
HO
NH2
Column:HO
NH 2
0 0.5 1 1.5 2 2.5 3 Temperature: 45 °C
0 0.5 1 1.5 2 2.5 3
Clenbuterol
OH
CI
H
N OH
Resolution: 3.70 H
H2N CI N
CI
Method Conditions
0 1 2 3 4 5
Column:H2N InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
Time (min)
Mobile phase: CI100/0.2/0.05: Methanol/Acetic Acid/Ammonium Hydroxide
0 1 2 3 4 5
Time (min)
Clenbuterol
OH
OH
H Resolution: 2.66 H
CI N
CI N
H2N
CI Method Conditions
HN
Column: 2 InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 1 2 3 4 5 Mobile phase: CI100/0.1/0.02: Methanol/Acetic Acid/Ammonium Hydroxide
0 1 2 3 4 5
Time (min)
71
Stimulants
Ethylone
Retention (min): O (Peak1/Peak 2)
13.38/14.18
O
Resolution: 1.50
O N
O N
O
Method Conditions
Column:
O InfinityLab Poroshell 120 Chiral-T (10 cm x 4.6 mm, 2.7 μm)
0 5 10 15 20 25 Mobile phase: 100/0.2/0.05: Methanol/Acetic Acid/Ammonium Hydroxide
0 5 10 15 20 25 Detection: UV 230 nm
Time (min)
Ethylone
Resolution: 2.61 O
H
O
O
H
N O N
Method Conditions
O
Column:
O
Mobile phase:
0 2 4 6 8
Time (min)
Methylphenidate
Resolution: 1.58
O O
H
O O
N Method Conditions
Column:
H
0 2 4
Time (min)
6 8 10
Mobile phase: N
0 2 4 6 8 10
Time (min)
Methylphenidate
Resolution: 5.18
O O
O O
H
N Method Conditions H
Column: InfinityLabN
Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 2 4 6 8 10 Mobile phase: 100/0.1 wt %: Methanol/Ammonium Formate (pH 3.6)
0 0.5 1 1.5
Time (min)
72
Stimulants
3-fluoromethcathinone
Resolution: 1.50 O
H
F
O
H
N
F N
Method Conditions
0 2 4 6 8 10 12
0 2 4 6 8 10 12
Time (min)
3,4-methylenedioxy-N-ethylamphetamine (MDEA)
Resolution: 1.58 H
H O N
O N
Method Conditions
O
O
0 2 4 6 8 10 12 Flow Rate: 0.5 mL/min
0 2 4 6 8 10 12
4-ethylmethcathinone
H O
N Resolution: 1.74
H
N
Method Conditions
0 5 10 15
0 5 10 15
H
N
Resolution: O
2.23
H
N
Method Conditions
0 5 10
Time (min)
0 5 10
Time (min)
73
Stimulants
Aminorex
Resolution: 1.98 N
N NH2
O
NH2
Method Conditions O
0 2 4 6 8 10
0 2 4 6 8 10
Amphetamine
Resolution: 2.48
NH2 NH2
Method Conditions
Flow Rate:
0 1 2 3 4
Time (min)
1.0 mL/min
0 1 2 3 4
Time (min)
Butylone
Retention (min):
Resolution: NH1.97 O
5.71/6.10 (Peak1/Peak 2)
NH O
O Method Conditions O
O Column:
Mobile phase:
OInfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
8 10 Temperature: Ambient (23 °C)
0 2 4 6 8 10
Time (min)
Cathinone
Retention (min):
Resolution:
O
2.01/2.23 (Peak1/Peak 2)
2.65
O

Column: NH
2
1.5 2 2.5 3
ime (min)
Temperature: 15 °C
0 0.5 1 1.5 2 2.5 3 Detection: UV 220 nm
Time (min)
74
Stimulants
Flephedrone
O
O
H
Resolution: 1.85
H
N N
Method Conditions
F
F
0 2 4 6 8 10 Flow Rate: 0.5 mL/min
0 2 4 6 8 10
Time (min)
Mephedrone
O
O
Resolution: 1.84
H
H
N N
Method Conditions
0 5 10 15
Time (min)
Time (min)
Methamphetamine
Resolution: 2.73
N
H
N
Method Conditions
Column: H InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
0 2 4 6 8 Mobile phase: 100/0.1/0.05: Methanol/Acetic Acid/Triethylamine
0 2 4 6 8
Time (min)
Methylephedrine
Resolution: 1.92
N
OH
N
Method Conditions
0 1 2 3
Time (min)
4 5 6 7
Mobile phase: OH
0 1 2 3 4 5 6 7
Time (min)
75
Stimulants
Methylone
O
H Retention (min): O (Peak1/Peak 2)
6.52/7.03
O N Resolution: 2.08 H
O O N
Method Conditions
4 6 8 10
Column: O InfinityLab Poroshell 120 Chiral-V (10 cm x 4.6 mm, 2.7 μm)
Time (min)
0 2 4 6 8 10 Detection: UV 230 nm
Time (min)
p-methoxyamphetamine
NH2 NH2
Method Conditions
0 1 2 3 4 5 6
Time (min)
0 1 2 3 4 5 6
Time (min)
Pentylone
NH O Retention (min):
NH O
8.88/9.55 (Peak1/Peak 2)
Resolution: 1.72
O
O O
Method Conditions
Column:
O
0 5 10 15 Mobile phase: 90/10: Ethanol/15 mM Ammonium Formate (pH 3.6)
0 5 10 15
Time (min)
Synephrine
OH
Resolution: 1.92
H
OH
H
N
N
Method Conditions
HO
HO
0 5 10 15 20 Flow Rate: 0.5 mL/min
0 5 10 15 20
Time (min)
76
Stimulants
α-pyrrolidinopentiophenone
O Resolution: O
4.33
N
N
Method Conditions
0 0.5 1 1.5 2 2.5 3
0 0.5 1 1.5 2 2.5 3
Time (min)
77
Table of Contents by Column
InfinityLab Poroshell 120 Chiral-CD Tryptophanol........................................................50 Pindolol..................................................................37

1-benzyl-2,2-diphenylethylamine................... 10 Tyrosinol................................................................50 Propanolol............................................................38
1,1'-binaphthyl-2,2'-diamine............................. 10 α-(1-aminoethyl)-4-hydroxybenzyl- Salbutamol...........................................................38
Methadone........................................................... 24 alcohol...................................................................50 Sotalol....................................................................39
Bupropion............................................................. 31 Octopamine..........................................................53 Terbutaline............................................................39
Temazepam......................................................... 31 Norephedrine.......................................................70 Tulobuterol............................................................40
Chlorthalidone..................................................... 42 Normetanephrine...............................................70 Baclofen................................................................ 41
Benalaxyl...............................................................43 Norphenylephrine...............................................71 Bendroflumethazide.......................................... 42
Econazole.............................................................43 InfinityLab Poroshell 120 Chiral-T Chlorthalidone..................................................... 42
Flutriafol................................................................43 2-amino-2-pheylbutyric acid........................... 18 Dichloroprop........................................................44
Miconazole...........................................................43 4-nitrophenylalanine.......................................... 18 Fenoprop...............................................................44
Praziquantel.........................................................44 AQC Methionine.................................................. 18 Haloxyfop..............................................................45
Prothioconazole..................................................44 AQC Phenylalanine............................................. 18 Mecoprop methyl ester....................................45
2'-amino-1,1'-binaphthalen-2-ol...................... 47 AQC Serine............................................................ 19 Mecoprop ...........................................................45
Hydratropic acid.................................................. 47 AQC Tyrosine....................................................... 19 Bromacil................................................................46
Jacobsen's catalyst........................................... 47 Fmoc Alanine....................................................... 19 Napropamid.........................................................46
Tropic acid............................................................ 47 Fmoc Asparagine............................................... 19 2-(2-chlorophenoxy)propionic acid...............50
α-N,N-dimethylaminophenyl acetonitrile....48 Fmoc Leucine......................................................20 2-(3-chlorophenoxy)propionic acid............... 51
1,5-dimethyl-4-phenyl-2-imidazolidinone....54 Fmoc Phenylalanine..........................................20 2-(4-chlorphenoxy)propionic acid................. 51
1-indanol................................................................57 Fmoc Tryptophan...............................................20 2-phenoxypropanoic acid................................ 51
Benzoin..................................................................57 Fmoc Valine..........................................................20 4-benzyl-2-oxazolidinone.................................54
Chlophedianol......................................................57 Methionine............................................................ 21 4-methyl-5-phenyl-2-oxazolidinone..............54
Homatropine........................................................58 p-chlorophenylalanine ..................................... 21 4-phenyl-2-oxazolidinone.................................54
Indapamide..........................................................58 p-fluorophenylalanine....................................... 21 5,5-dimethyl-4-phenyl-2-oxazolidinone....... 55
Lofexidine..............................................................58 Phenylalanine...................................................... 21 5,5-dimethyl-4-benzyl-2-oxazolidinone....... 55
p-chloramphetamine........................................59 Tryptophan...........................................................22 5,5-dimethyl-4-methyl-2-oxazolidinone....... 55
Phenylsuccinic anhydride................................59 α-amino-2-thiophenacetic acid......................22 5-methyl-5-phenylhydantoin...........................59
Triadimenol...........................................................59 3,4-dihydroxyphenylalanine (DOPA) ............ 23 Aminoglutethimide............................................60
3,4-methylenedioxyamphetamine (MDA)... 67 Proglumide........................................................... 24 Bamethan.............................................................60
3,4-methylenedioxymethamphetamine Indoprofen............................................................ 25 Diperodon.............................................................60
(MDMA).................................................................67 Flurbiprofen.......................................................... 61
Ketorolac............................................................... 25
3,4-dimethylmethcathinone............................67 Mandelic acid....................................................... 61
Hexobarbital.........................................................27
4-methylethcathinone.......................................68 Naftopidil............................................................... 61
Mepivacaine.........................................................27
Methoxamine.......................................................68 p-hydroxymandelic acid................................... 62
Coumachlor..........................................................29
Pentedrone...........................................................69 Pseudoephedrine ..............................................69
Warfarin.................................................................29
Pseudoephedrine...............................................69 3,4-dihydroxyphenylalanine (DOPA)............. 71
Phensuximide......................................................30
α-pyrrolidinopentiophenone............................70 Clenbuterol...........................................................71
α-methyl-α-phenylsuccinimide......................30
InfinityLab Poroshell 120 Chiral-CF Lorazepam............................................................ 31 Ethylone.................................................................72
1-(1-naphthyl)ethylamine................................. 11 Lormetazepam.................................................... 31 Methylphenidate.................................................72
1-(1,1-biphenyl-4-yl)ethanamine..................... 11 Mianserin..............................................................32 4-methylethcathinone.......................................73
1-(4-methoxyphenyl)ethylamine.................... 12 Oxazepam.............................................................32 InfinityLab Poroshell 120 Chiral-V
1-aminotetralin.................................................... 12 Acebutolol.............................................................34 -benzyl-2,2-diphenylethylamine................... 10
1
6-methoxy-1,2,3,4-tetrahydro-1- Alprenolol..............................................................34 1,1'-binaphthyl-2,2'-diamine............................. 10
naphthalenylamine............................................ 13 Atenolol..................................................................35 1-(1,1-biphenyl-4-yl)ethanamine..................... 11
2-amino-1,2-diphenylethanol..........................48 Esmolol..................................................................35 1-(1-naphthyl)ethylamine ................................ 11
phenylalaninol......................................................48 Isoproterenol .......................................................36 1-(4-methoxyphenyl)ethylamine.................... 12
4-chlorophenylalaninol..................................... 49 Metaproterenol....................................................36 1-aminotetralin.................................................... 12
trans-1-amino-2-indanol................................... 49 Metoprolol.............................................................37 1-(2-methoxyphenyl)ethanamine.................. 13
78
1-(4-chlorophenyl)ethylamine......................... 13 Mandipropamid...................................................45 Mephedrone......................................................... 75
6-methoxy-1,2,3,4-tetrahydro-1- Bromacil................................................................46 Methamphetamine............................................ 75
naphthalenylamine............................................ 13 Napropamid.........................................................46 Methylephedrine................................................. 75
1,1-diphenyl-1-fluoro-2-aminopropane......... 14 2-amino-1,2-diphenylethanol..........................48 Methylone............................................................. 76
1,1-diphenyl-2-aminopropane......................... 14 phenylalaninol...................................................... 49 p-methoxyamphetamine................................. 76
1,2,2-triphenylethylamine................................. 14 trans-1-amino-2-indanol................................... 49 Pentylone.............................................................. 76
2-amino-4-methyl-1,1-diphenylpentane 2-amino-1,1,-diphenyl-1-propanol................... 51 Synephrine........................................................... 76
propane................................................................. 14 2-amino-1,1,13-triphenyl-1-propanol.............. 52 α-pyrrolidinopentiophenone............................77
2-chloro-indan-1-ylamine................................. 15 2-(carbobenzyloxyamino)-3-phenyl-1-
4-methoxy-α-methylbenzylamine................. 15 propanol................................................................ 52
bis(1-phenylethyl)amine .................................. 15 cis-1-amino-2-indanol........................................ 52
bis(α-methylbenzyl)amine............................... 15 (3a-cis)-3,3a,8,8a-tetrahydro-2H-
Mexiletine.............................................................. 16 indeno[1,2]doxazol2-one...................................55
N-benzyl-α-methylbenzylamine.................... 16 4-(1-H-indol-3-yl-methyl)-2-oxazolidinone....56
N,α-dimethylbenzylamine................................ 16 Chlophedianol......................................................57
α-methyl-4-nitrobenzylamine......................... 16 Lofexidine..............................................................58
α-methylbenzylamine....................................... 17 Bamethan.............................................................60
3-amino-3-phenylpropanoic acid..................22 Diperodon............................................................. 61
Kynurenine............................................................22 Amlodipine............................................................ 62
Tryptophanamide...............................................23 Brompheniramine.............................................. 62
Methadone........................................................... 24 Carbinoxamine.................................................... 62
Proglumide........................................................... 24 Cetirizine................................................................63
Methorphan......................................................... 25 Chlorpheniramine...............................................63
Nefopam............................................................... 25 Citalopram ...........................................................63
Tramadol...............................................................26 Disopyramide.......................................................63
Hexobarbital.........................................................27 Ketamine...............................................................64
Mepivacaine.........................................................27 Methoxyphenamine...........................................64
Bupivicaine...........................................................28 Methoxyverapamil.............................................64
Mephobarbital.....................................................28 Nicardipidine........................................................64
Prilocaine..............................................................28 Orphenadrine.......................................................65
Phensuximide......................................................30 Piperoxan..............................................................65
Fluoxetine.............................................................32 Promethazine......................................................65
Mianserin..............................................................32 Tetrahydrozoline.................................................65
Tranylcypromine.................................................33 Thalidomide.........................................................66
Trimipramine........................................................33 Triahexylphenidyl................................................66
Venlafaxine...........................................................33 Trimebutine..........................................................66
Acebutolol.............................................................34 Verapamil..............................................................66
Alprenolol..............................................................34 3,4-methylenedioxyamphetamine (MDA).... 67
Atenolol..................................................................35 Methoxamine.......................................................68
Esmolol..................................................................35 Pentedrone...........................................................69
Isoproterenol .......................................................36 Pseudoephedrine...............................................70
Metaproterenol....................................................36 Clenbuterol...........................................................71
Metoprolol.............................................................37 Ethylone.................................................................72
Pindolol..................................................................37 Methylphenidate.................................................72
Propanolol............................................................38 3-fluoromethcathinone..................................... 73
Salbutamol...........................................................38 3,4-methylenedioxy-N-ethylamphetamine
Sotalol....................................................................39 (MDEA)..................................................................73
Terbutaline............................................................39 4-ethylmethcathinone.......................................73
Carvedilol..............................................................40 4-methylethcathinone.......................................73
Tulobuterol............................................................40 Aminorex............................................................... 74
Baclofen................................................................ 41 Amphetamine...................................................... 74
Octoclothepin...................................................... 41 Butylone ............................................................... 74
Tolperisone........................................................... 41 Cathinone.............................................................. 74
Flephedrone......................................................... 75
79
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Published in the USA, December 1, 2017
5991-8450EN

5991-8450EN Chiral Columns Appcompendium Compressed

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Traditional Chiral Separation— Agilent InfinityLab Poroshell 120 Chiral Separation—

1. D-(+)-Amphetamine, 2. L-(-)-Amphetamine, 3. D-(+)-Methamphetamine 1. D-(+)-Amphetamine, 2. L-(-)-Amphetamine, 3. D-(+)-Methamphetamine

Polar Organic (PO) Primary amines

Reversed Phase (RP) Stimulants, fungicides, t-boc amino acids

Polar Ionic (PI) Basic pharmaceuticals (various)

Polar Organic (PO) Complex neutral molecules

Polar Ionic (PI) Beta blockers, hydroxyl acids

Polar Organic (PO) Hydantoins, benzodiazepines

RP = Reversed phase PI = Polar iconic PO = Polar organic NP = Normal phase

Part number Column description Part number Column description

Learn more about detecting enantiomers quickly when time is short.

Chiral screening protocol

2 MeOH/20 mM ammonium formate, pH 4.0 30/70 RP

7 EtOH/Heptane/Acetic Acid/TEA 20/80/0.3/0.2 NP

RP = Reversed phase PI = Polar iconic PO = Polar organic NP = Normal phase

Normal phase mode (NP): Typically non-

InfinityLab Poroshell 120 Chiral-CF 400 bar 3-7 45 °C

InfinityLab Poroshell 120 Chiral-CD 400 bar 3-7 45 °C

InfinityLab Poroshell 120 Chiral-V 400 bar 2.5-7 45 °C

InfinityLab Poroshell 120 Chiral-T 400 bar 2.5-7 45 °C

Agilent Poroshell 120 Chiral

Don’t just produce data… find answers

Agilent InfinityLab: Maximum efficiency for your LC workflow

Agilent InfinityLab Supplies: Save time and minimize troubleshooting

InfinityLab Quick Turn fittings

Like all InfinityLab Poroshell 120 columns, Poroshell

Amines Analgesics Depressants

NH2 Method Conditions

NH2 Method Conditions

Flow Rate: 1.0 mL/min 2

O Retention (min): 16.25/17.10 (Peak1/Peak 2)

O Retention (min): 2.62/2.89 (Peak1/Peak 2)

O Retention (min): 2.92/3.29 (Peak1/Peak 2)

Mecoprop methyl ester

2-(3-chlorophenoxy)propionic acid (Metachlorphenprop)

Retention (min): 1.67/1.82 (Peak1/Peak 2)

NH2 Method Conditions

InfinityLab Poroshell 120 Chiral-CD Tryptophanol........................................................50 Pindolol..................................................................37

U.S. and Canada

For Research Use Only. Not for use in diagnostic procedures.

This information is subject to change without notice.

© Agilent Technologies, Inc. 2017

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