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    Lab Report Template - Exp1 SB015 2022 - Student's

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    23005852
    This document outlines an experiment on basic microscopy techniques. The objectives are to obtain accurate images, determine depth of field and field of view, calculate actual magnification, and apply oil immersion with high magnification. Students will observe slides with different lenses, note observations, calculate magnifications and fields of view, compare depth of field between lenses, and use oil immersion with high magnification. Questions at the end test understanding of microscope parts and techniques.

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    Download as DOCX, PDF, TXT or read online from Scribd

    Lab Report Template - Exp1 SB015 2022 - Student's

    Uploaded by

    23005852
    77 views7 pages
    This document outlines an experiment on basic microscopy techniques. The objectives are to obtain accurate images, determine depth of field and field of view, calculate actual magnification, and apply oil immersion with high magnification. Students will observe slides with different lenses, note observations, calculate magnifications and fields of view, compare depth of field between lenses, and use oil immersion with high magnification. Questions at the end test understanding of microscope parts and techniques.

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    This document outlines an experiment on basic microscopy techniques. The objectives are to obtain accurate images, determine depth of field and field of view, calculate actual magnification, and apply oil immersion with high magnification. Students will observe slides with different lenses, note observations, calculate magnifications and fields of view, compare depth of field between lenses, and use oil immersion with high magnification. Questions at the end test understanding of microscope parts and techniques.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    77 views7 pages

    Lab Report Template - Exp1 SB015 2022 - Student's

    Uploaded by

    23005852
    This document outlines an experiment on basic microscopy techniques. The objectives are to obtain accurate images, determine depth of field and field of view, calculate actual magnification, and apply oil immersion with high magnification. Students will observe slides with different lenses, note observations, calculate magnifications and fields of view, compare depth of field between lenses, and use oil immersion with high magnification. Questions at the end test understanding of microscope parts and techniques.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 7

    Student’s name : ______________________ Class: ____________

    Lecturer’s name : ______________________

    EXPERIMENT 1: BASIC TECHNIQUES IN MICROSCOPY

    Objectives

    i. To obtain accurate images


    ii. To determine the depth of field
    iii. To determine the field of view
    iv. To calculate the actual magnification
    v. To apply the use of oil immersion with high magnification (oil immersion lens)

    Exercise 1.1: Images, Depth of Fields and Field of View of The Microscope

    1.1.1 Images under the microscope

    Apparatus
    Compound light microscope

    Materials
    ‘e’ prepared slide

    Procedures
    1. The ‘e’ prepared slide is observed using the 4x objective lens.
    2. Observation under 4x objective lens is drawn.
    3. The position of ‘e’ is determined.

    Observation

    ‘e’ image

    (Take a photo of your observation & paste here)

    Actual magnification: ________

    Conclusion:

    The letter ‘e’ image is ________ and ________.

    Unit Biology KMPk Page 1 of 7


    Student’s name : ______________________ Class: ____________
    Lecturer’s name : ______________________

    1.1.2 The depth of field

    Apparatus
    (List down the apparatus)

    Materials
    (List down the materials)

    Procedures
    (Write in passive sentences)

    Observation

    As for your reference, the above diagram shows the cross thread slide observed with naked
    eyes and using 4x, 10x and 40x objective lens.
    Follow the procedures given in the lab manual and state your observation in the table below.

    Position of the naked eyes Identify the colour of thread


    thread on the under the microscope using
    slide 4x objective 10x objective 40x objective
    lens. lens. lens.
    at the top
    in the middle
    at the bottom

    Conclusion:

    Unit Biology KMPk Page 2 of 7


    Student’s name : ______________________ Class: ____________
    Lecturer’s name : ______________________

    The ________ the magnification, the ________ the depth of field.

    1.1.3 The field of view

    Apparatus
    (List down the apparatus)

    Materials
    (List down the materials)

    Procedures
    (Write in passive sentences)

    Observation

    (Title of your observation)

    (Take a photo of your observation & paste here)

    Actual magnification: 40x

    Determine: (Show your calculation for question 2 & 3)

    1. Diameter of field of view for the 4x objective lens is ______ mm or ______ µm.

    2. Diameter of field of view for the 10x =______ mm = _______ µm.

    3. The diameter of field of view for the 40x = ______mm = ______ µm.

    Conclusion:

    Unit Biology KMPk Page 3 of 7


    Student’s name : ______________________ Class: ____________
    Lecturer’s name : ______________________

    The ________ the magnification, the ________ the size of the field of view.

    Exercise 1.2: Magnification

    Procedure
    (Write in passive sentences)

    Observation

    Table 1.1 Actual magnification of a specimen

    Actual magnification
    Magnification power of Magnification power of objective lens
    ocular lens
    4x 10x 40x 100x
    10x

    Conclusion:

    Using the 10x magnification power of ________ lens, the greater the magnification power of
    ________ lens will cause the actual magnification to ________.

    Unit Biology KMPk Page 4 of 7


    Student’s name : ______________________ Class: ____________
    Lecturer’s name : ______________________

    Exercise 1.3: Oil Immersion Objective Lens

    Apparatus
    (List down the apparatus)

    Materials
    (List down the materials)

    Procedures
    (Write in passive sentences)

    Observation:

    (Title of your observation)

    (Take a photo of your observation & paste here)

    Actual magnification: ________

    Conclusion:

    Oil immersion techniques using higher magnification (________ objective lens), where oils
    ________ light refraction. The ________ the resolution of the microscope, the ________ is
    the ability to distinguish details of the object.

    Unit Biology KMPk Page 5 of 7


    Student’s name : ______________________ Class: ____________
    Lecturer’s name : ______________________

    Questions:

    Part A

    For questions 1 to 7, choose the correct answer from the following list:
    A Scanning objective lens (4x)
    B Low-power objective lens (10x)
    C High-power objective lens (40x)
    D Oil immersion objective lens (100x)

    1. Which is the shortest objective lens? ( _________ )


    2. Which objective lens should you use when you begin to focus a specimen? ( __________ )
    3. Which objective lens should be in position before you store a microscope? ( __________ )
    4. Which objective lens will deliver the highest amount of light? ( __________ )
    5. Which objective lens requires immersion oil to fill up the space between the specimen and
    the lens? ( __________ )
    6. Which objective lens will remain in focus when placed at the longest working distance
    from the specimen? ( __________ )
    7. When using an ocular lens with 10x magnification power, which objective lens should be
    used to obtain the following actual magnification?
    (a) 100 times of its diameter. ( __________ )
    (b) 1000 times of its diameter. ( __________ )

    Part B

    1. Using 40x objective lens, determine the length and width of a cell from a piece of cork
    tissue with approximately 20 cells in horizontal position and 10 cells in vertical position.

    Figure 1.4 Cork tissue

    2. Based on laboratory practices, what do you use to clean the microscope lenses?

    Unit Biology KMPk Page 6 of 7


    Student’s name : ______________________ Class: ____________
    Lecturer’s name : ______________________

    3. While observing a moving microorganism under a microscope, you found that the
    organism has moved out of the field of view to the right. In order to keep observing the
    microorganism, which direction do you move your slide (right/left)?

    4. How do you adjust the slide when the specimen is out of the field of view to the top?

    Part C

    1. Complete the following sentences.

    a. A microscope is called a compound microscope when it consists of more than one set
    of _________________________.

    b. Condenser and iris diaphragm are useful to coordinate ______________________.

    References: (minimum 2- use APA format)

    Unit Biology KMPk Page 7 of 7

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